P97571

PR
Gene name
Capn1
Protein name
Calpain-1 catalytic subunit
Names
Calcium-activated neutral proteinase 1, CANP 1, Calpain mu-type, Calpain-1 large subunit, Micromolar-calpain, muCANP
Species
Rattus norvegicus (Rat)
KEGG Pathway
rno:29153
EC number
3.4.22.52: Cysteine endopeptidases
Protein Class

Descriptions

The autoinhibited protein was predicted that may have potential autoinhibitory elements via cis-regPred.

Autoinhibitory domains (AIDs)

1-51 (Predicted disordered autoinhibitory region) PR

Target domain

Relief mechanism

Assay

cis-regPred

Accessory elements

No accessory elements

References

Autoinhibited structure

Activated structure

11 structures for P97571

Entry ID Method Resolution Chain Position Source
1KXR X-ray 207 A A/B 27-356 PDB
1QXP X-ray 280 A A/B 60-647 PDB
1TL9 X-ray 180 A A 27-356 PDB
1TLO X-ray 190 A A 27-356 PDB
2G8E X-ray 225 A A 27-356 PDB
2G8J X-ray 161 A A 27-356 PDB
2NQG X-ray 204 A A 27-356 PDB
2NQI X-ray 204 A A 27-356 PDB
2R9C X-ray 180 A A 27-356 PDB
2R9F X-ray 160 A A 27-356 PDB
AF-P97571-F1 Predicted AlphaFoldDB

No variants for P97571

Variant ID(s) Position Change Description Diseaes Association Provenance
No variants for P97571

No associated diseases with P97571

8 regional properties for P97571

Type Name Position InterPro Accession
active_site Cysteine peptidase, cysteine active site 109 - 120 IPR000169
domain Peptidase C2, calpain, catalytic domain 37 - 362 IPR001300
domain EF-hand domain 557 - 613 IPR002048-1
domain EF-hand domain 614 - 649 IPR002048-2
binding_site EF-Hand 1, calcium-binding site 627 - 639 IPR018247
domain Peptidase C2, calpain, large subunit, domain III 371 - 513 IPR022682
domain Peptidase C2, calpain, domain III 365 - 521 IPR022683
domain Calpain subdomain III 366 - 523 IPR033883

Functions

Description
EC Number 3.4.22.52 Cysteine endopeptidases
Subcellular Localization
  • Cytoplasm
  • Cell membrane
  • Translocates to the plasma membrane upon Ca(2+) binding
PANTHER Family
PANTHER Subfamily
PANTHER Protein Class
PANTHER Pathway Category No pathway information available

7 GO annotations of cellular component

Name Definition
cornified envelope A type of plasma membrane that has been modified through addition of distinct intracellular and extracellular components, including ceramide, found in cornifying epithelial cells (corneocytes).
cytoplasm The contents of a cell excluding the plasma membrane and nucleus, but including other subcellular structures.
cytosol The part of the cytoplasm that does not contain organelles but which does contain other particulate matter, such as protein complexes.
lysosome A small lytic vacuole that has cell cycle-independent morphology found in most animal cells and that contains a variety of hydrolases, most of which have their maximal activities in the pH range 5-6. The contained enzymes display latency if properly isolated. About 40 different lysosomal hydrolases are known and lysosomes have a great variety of morphologies and functions.
membrane A lipid bilayer along with all the proteins and protein complexes embedded in it an attached to it.
mitochondrion A semiautonomous, self replicating organelle that occurs in varying numbers, shapes, and sizes in the cytoplasm of virtually all eukaryotic cells. It is notably the site of tissue respiration.
plasma membrane The membrane surrounding a cell that separates the cell from its external environment. It consists of a phospholipid bilayer and associated proteins.

5 GO annotations of molecular function

Name Definition
calcium ion binding Binding to a calcium ion (Ca2+).
calcium-dependent cysteine-type endopeptidase activity Catalysis of the hydrolysis of nonterminal peptide bonds in a polypeptide chain by a mechanism using a cysteine residue at the enzyme active center, and requiring the presence of calcium.
cytoskeletal protein binding Binding to a protein component of a cytoskeleton (actin, microtubule, or intermediate filament cytoskeleton).
enzyme binding Binding to an enzyme, a protein with catalytic activity.
peptidase activity Catalysis of the hydrolysis of a peptide bond. A peptide bond is a covalent bond formed when the carbon atom from the carboxyl group of one amino acid shares electrons with the nitrogen atom from the amino group of a second amino acid.

17 GO annotations of biological process

Name Definition
aging A developmental process that is a deterioration and loss of function over time. Aging includes loss of functions such as resistance to disease, homeostasis, and fertility, as well as wear and tear. Aging includes cellular senescence, but is more inclusive. May precede death and may succeed developmental maturation (GO:0021700).
brain development The process whose specific outcome is the progression of the brain over time, from its formation to the mature structure. Brain development begins with patterning events in the neural tube and ends with the mature structure that is the center of thought and emotion. The brain is responsible for the coordination and control of bodily activities and the interpretation of information from the senses (sight, hearing, smell, etc.).
cellular response to hydrogen peroxide Any process that results in a change in state or activity of a cell (in terms of movement, secretion, enzyme production, gene expression, etc.) as a result of a hydrogen peroxide (H2O2) stimulus.
mammary gland involution The tissue remodeling that removes differentiated mammary epithelia during weaning.
negative regulation of actin filament polymerization Any process that stops, prevents, or reduces the frequency, rate or extent of actin polymerization.
negative regulation of NIK/NF-kappaB signaling Any process that stops, prevents or reduces the frequency, rate or extent of NIK/NF-kappaB signaling.
positive regulation of cardiac muscle cell apoptotic process Any process that increases the rate or extent of cardiac cell apoptotic process, a form of programmed cell death induced by external or internal signals that trigger the activity of proteolytic caspases whose actions dismantle a cardiac muscle cell and result in its death.
positive regulation of leukocyte tethering or rolling Any process that activates or increases the frequency, rate or extent of leukocyte tethering or rolling.
positive regulation of vascular permeability Any process that increases the extent to which blood vessels can be pervaded by fluid.
protein autoprocessing Processing which a protein carries out itself. This involves actions such as the autolytic removal of residues to generate the mature form of the protein.
protein catabolic process The chemical reactions and pathways resulting in the breakdown of a protein by the destruction of the native, active configuration, with or without the hydrolysis of peptide bonds.
proteolysis The hydrolysis of proteins into smaller polypeptides and/or amino acids by cleavage of their peptide bonds.
receptor catabolic process The chemical reactions and pathways resulting in the breakdown of a receptor molecule, a macromolecule that undergoes combination with a hormone, neurotransmitter, drug or intracellular messenger to initiate a change in cell function.
regulation of catalytic activity Any process that modulates the activity of an enzyme.
response to angiotensin Any process that results in a change in state or activity of a cell or an organism (in terms of movement, secretion, enzyme production, gene expression, etc.) as a result of an angiotensin stimulus. Angiotensin is any of three physiologically active peptides (angiotensin II, III, or IV) processed from angiotensinogen.
response to arsenic-containing substance Any process that results in a change in state or activity of a cell or an organism (in terms of movement, secretion, enzyme production, gene expression, etc.) as a result of an arsenic stimulus from compounds containing arsenic, including arsenates, arsenites, and arsenides.
self proteolysis The hydrolysis of proteins into smaller polypeptides and/or amino acids by cleavage of their own peptide bonds.

19 homologous proteins in AiPD

UniProt AC Gene Name Protein Name Species Evidence Code
Q27971 CAPN2 Calpain-2 catalytic subunit Bos taurus (Bovine) PR
Q27970 CAPN1 Calpain-1 catalytic subunit Bos taurus (Bovine) PR
P00789 Calpain-1 catalytic subunit Gallus gallus (Chicken) PR
Q9VXH6 CalpC Calpain-C Drosophila melanogaster (Fruit fly) PR
O14815 CAPN9 Calpain-9 Homo sapiens (Human) PR
Q6MZZ7 CAPN13 Calpain-13 Homo sapiens (Human) PR
Q9HC96 CAPN10 Calpain-10 Homo sapiens (Human) PR
P07384 CAPN1 Calpain-1 catalytic subunit Homo sapiens (Human) PR
Q9ESK3 Capn10 Calpain-10 Mus musculus (Mouse) PR
G3UZ78 Adgb Androglobin Mus musculus (Mouse) PR
O08529 Capn2 Calpain-2 catalytic subunit Mus musculus (Mouse) PR
Q9D805 Capn9 Calpain-9 Mus musculus (Mouse) PR
Q3UW68 Capn13 Calpain-13 Mus musculus (Mouse) PR
O35350 Capn1 Calpain-1 catalytic subunit Mus musculus (Mouse) PR
P43367 CAPN2 Calpain-2 catalytic subunit Sus scrofa (Pig) PR
P35750 CAPN1 Calpain-1 catalytic subunit Sus scrofa (Pig) PR
Q07009 Capn2 Calpain-2 catalytic subunit Rattus norvegicus (Rat) PR
O35920 Capn9 Calpain-9 Rattus norvegicus (Rat) PR
Q5BK10 Capn13 Calpain-13 Rattus norvegicus (Rat) PR
10 20 30 40 50 60
MAEELITPVY CTGVSAQVQK QRDKELGLGR HENAIKYLGQ DYENLRARCL QNGVLFQDDA
70 80 90 100 110 120
FPPVSHSLGF KELGPNSSKT YGIKWKRPTE LLSNPQFIVD GATRTDICQG ALGDCWLLAA
130 140 150 160 170 180
IASLTLNETI LHRVVPYGQS FQEGYAGIFH FQLWQFGEWV DVVVDDLLPT KDGKLVFVHS
190 200 210 220 230 240
AQGNEFWSAL LEKAYAKVNG SYEALSGGCT SEAFEDFTGG VTEWYDLQKA PSDLYQIILK
250 260 270 280 290 300
ALERGSLLGC SINISDIRDL EAITFKNLVR GHAYSVTDAK QVTYQGQRVN LIRMRNPWGE
310 320 330 340 350 360
VEWKGPWSDN SYEWNKVDPY EREQLRVKME DGEFWMSFRD FIREFTKLEI CNLTPDALKS
370 380 390 400 410 420
RTLRNWNTTF YEGTWRRGST AGGCRNYPAT FWVNPQFKIR LEEVDDADDY DSRESGCSFL
430 440 450 460 470 480
LALMQKHRRR ERRFGRDMET IGFAVYQVPR ELAGQPVHLK RDFFLANASR AQSEHFINLR
490 500 510 520 530 540
EVSNRIRLPP GEYIVVPSTF EPNKEGDFLL RFFSEKKAGT QELDDQIQAN LPDEKVLSEE
550 560 570 580 590 600
EIDDNFKTLF SKLAGDDMEI SVKELQTILN RIISKHKDLR TNGFSLESCR SMVNLMDRDG
610 620 630 640 650 660
NGKLGLVEFN ILWNRIRNYL TIFRKFDLDK SGSMSAYEMR MAIEAAGFKL NKKLHELIIT
670 680 690 700 710
RYSEPDLAVD FDNFVCCLVR LETMFRFFKI LDTDLDGVVT FDLFKWLQLT MFA