P84091
PR
Gene name |
Ap2m1 (Clapm1) |
Protein name |
AP-2 complex subunit mu |
Names |
AP-2 mu chain, Adaptor protein complex AP-2 subunit mu, Adaptor-related protein complex 2 subunit mu, Clathrin assembly protein complex 2 mu medium chain, Clathrin coat assembly protein AP50, Clathrin coat-associated protein AP50, Mu2-adaptin, Plasma membrane adaptor AP-2 50 kDa protein |
Species |
Mus musculus (Mouse) |
KEGG Pathway |
mmu:11773 |
EC number |
|
Protein Class |
|
Descriptions
The autoinhibited protein was predicted that may have potential autoinhibitory elements via cis-regPred.
Autoinhibitory domains (AIDs)
Target domain |
|
Relief mechanism |
|
Assay |
cis-regPred |
Accessory elements
No accessory elements
Autoinhibited structure
Activated structure
10 structures for P84091
| Entry ID | Method | Resolution | Chain | Position | Source |
|---|---|---|---|---|---|
| 6OWO | EM | 320 A | M | 1-435 | PDB |
| 6OWT | EM | 380 A | M | 1-141 | PDB |
| 6OXL | EM | 350 A | M | 1-435 | PDB |
| 7RW8 | EM | 350 A | M | 1-435 | PDB |
| 7RW9 | EM | 390 A | M | 1-435 | PDB |
| 7RWA | EM | 470 A | M/m | 1-435 | PDB |
| 7RWB | EM | 390 A | M/m | 1-435 | PDB |
| 7RWC | EM | 380 A | M | 1-435 | PDB |
| 8T1O | EM | 330 A | M | 1-435 | PDB |
| AF-P84091-F1 | Predicted | AlphaFoldDB |
22 variants for P84091
| Variant ID(s) | Position | Change | Description | Diseaes Association | Provenance |
|---|---|---|---|---|---|
| rs3389413354 | 19 | V>I | No | EVA | |
| rs3389403599 | 25 | G>R | No | EVA | |
| rs3389401061 | 44 | R>H | No | EVA | |
| rs3389416112 | 53 | T>N | No | EVA | |
| rs3389355963 | 133 | I>V | No | EVA | |
| rs3389355939 | 134 | T>A | No | EVA | |
| rs3389375817 | 138 | I>F | No | EVA | |
| rs3413041604 | 158 | Q>H | No | EVA | |
| rs3389413307 | 223 | E>* | No | EVA | |
| rs3389404728 | 228 | G>D | No | EVA | |
| rs3389387189 | 233 | T>S | No | EVA | |
| rs3389408013 | 244 | D>E | No | EVA | |
| rs3389398188 | 246 | C>G | No | EVA | |
| rs3389403618 | 257 | F>L | No | EVA | |
| rs3389423086 | 258 | D>N | No | EVA | |
| rs3389398224 | 259 | S>Y | No | EVA | |
| rs3389400074 | 320 | I>N | No | EVA | |
| rs3389416157 | 341 | K>E | No | EVA | |
| rs3389409864 | 345 | K>R | No | EVA | |
| rs3389387156 | 366 | I>N | No | EVA | |
| rs3389403686 | 402 | R>H | No | EVA | |
| rs3389416101 | 427 | R>H | No | EVA |
No associated diseases with P84091
1 regional properties for P84091
| Type | Name | Position | InterPro Accession |
|---|---|---|---|
| domain | GPCR, rhodopsin-like, 7TM | 40 - 440 | IPR017452 |
Functions
10 GO annotations of cellular component
| Name | Definition |
|---|---|
| AP-2 adaptor complex | A heterotetrameric AP-type membrane coat adaptor complex that consists of alpha, beta2, mu2 and sigma2 subunits, and links clathrin to the membrane surface of a vesicle, and the cargo receptors during receptor/clathrin mediated endocytosis. Vesicles with AP-2-containing coats are normally found primarily near the plasma membrane, on endocytic vesicles. In at least humans, the AP-2 complex can be heterogeneric due to the existence of multiple subunit isoforms encoded by different alpha genes (alphaA and alphaC). |
| clathrin-coated pit | A part of the endomembrane system in the form of an invagination of a membrane upon which a clathrin coat forms, and that can be converted by vesicle budding into a clathrin-coated vesicle. Coated pits form on the plasma membrane, where they are involved in receptor-mediated selective transport of many proteins and other macromolecules across the cell membrane, in the trans-Golgi network, and on some endosomes. |
| cytoplasmic side of plasma membrane | The leaflet the plasma membrane that faces the cytoplasm and any proteins embedded or anchored in it or attached to its surface. |
| cytoplasmic vesicle | A vesicle found in the cytoplasm of a cell. |
| glutamatergic synapse | A synapse that uses glutamate as a neurotransmitter. |
| mitochondrion | A semiautonomous, self replicating organelle that occurs in varying numbers, shapes, and sizes in the cytoplasm of virtually all eukaryotic cells. It is notably the site of tissue respiration. |
| plasma membrane | The membrane surrounding a cell that separates the cell from its external environment. It consists of a phospholipid bilayer and associated proteins. |
| postsynapse | The part of a synapse that is part of the post-synaptic cell. |
| presynapse | The part of a synapse that is part of the presynaptic cell. |
| secretory granule | A small subcellular vesicle, surrounded by a membrane, that is formed from the Golgi apparatus and contains a highly concentrated protein destined for secretion. Secretory granules move towards the periphery of the cell and upon stimulation, their membranes fuse with the cell membrane, and their protein load is exteriorized. Processing of the contained protein may take place in secretory granules. |
6 GO annotations of molecular function
| Name | Definition |
|---|---|
| clathrin adaptor activity | Bringing together a cargo protein with clathrin, responsible for the formation of endocytic vesicles. |
| disordered domain specific binding | Binding to a disordered domain of a protein. |
| lipid binding | Binding to a lipid. |
| low-density lipoprotein particle receptor binding | Binding to a low-density lipoprotein receptor. |
| signal sequence binding | Binding to a signal sequence, a specific peptide sequence found on protein precursors or mature proteins that dictates where the mature protein is localized. |
| transmembrane transporter binding | Binding to a transmembrane transporter, a protein or protein complex that enables the transfer of a substance, usually a specific substance or a group of related substances, from one side of a membrane to the other. |
13 GO annotations of biological process
| Name | Definition |
|---|---|
| clathrin-dependent endocytosis | An endocytosis process that begins when material is taken up into clathrin-coated pits, which then pinch off to form clathrin-coated endocytic vesicles. |
| endocytosis | A vesicle-mediated transport process in which cells take up external materials or membrane constituents by the invagination of a small region of the plasma membrane to form a new membrane-bounded vesicle. |
| intracellular protein transport | The directed movement of proteins in a cell, including the movement of proteins between specific compartments or structures within a cell, such as organelles of a eukaryotic cell. |
| negative regulation of protein localization to plasma membrane | Any process that stops, prevents or reduces the frequency, rate or extent of protein localization to plasma membrane. |
| positive regulation of receptor internalization | Any process that activates or increases the frequency, rate or extent of receptor internalization. |
| positive regulation of synaptic vesicle endocytosis | Any process that activates or increases the frequency, rate or extent of synaptic vesicle endocytosis. |
| postsynaptic neurotransmitter receptor internalization | A receptor-mediated endocytosis process that results in the internalization of a neurotransmitter receptor from the postsynaptic membrane endocytic zone into an endocytic vesicle. |
| protein-containing complex assembly | The aggregation, arrangement and bonding together of a set of macromolecules to form a protein-containing complex. |
| receptor internalization | A receptor-mediated endocytosis process that results in the movement of receptors from the plasma membrane to the inside of the cell. The process begins when cell surface receptors are monoubiquitinated following ligand-induced activation. Receptors are subsequently taken up into endocytic vesicles from where they are either targeted to the lysosome or vacuole for degradation or recycled back to the plasma membrane. |
| regulation of vesicle size | Any process that modulates the size of a vesicle. |
| synaptic vesicle endocytosis | A vesicle-mediated transport process, in which the synaptic vesicle membrane constituents are retrieved from the presynaptic membrane on the axon terminal after neurotransmitter secretion by exocytosis. Synaptic vesicle endocytosis can occur via clathrin-dependent and clathrin-independent mechanisms. |
| vesicle budding from membrane | The evagination of a membrane, resulting in formation of a vesicle. |
| vesicle-mediated transport | A cellular transport process in which transported substances are moved in membrane-bounded vesicles; transported substances are enclosed in the vesicle lumen or located in the vesicle membrane. The process begins with a step that directs a substance to the forming vesicle, and includes vesicle budding and coating. Vesicles are then targeted to, and fuse with, an acceptor membrane. |
8 homologous proteins in AiPD
| UniProt AC | Gene Name | Protein Name | Species | Evidence Code |
|---|---|---|---|---|
| P38700 | APM2 | Adaptin medium chain homolog APM2 | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) | PR |
| Q99186 | APM4 | AP-2 complex subunit mu | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) | PR |
| Q96CW1 | AP2M1 | AP-2 complex subunit mu | Homo sapiens (Human) | PR |
| Q9WVP1 | Ap1m2 | AP-1 complex subunit mu-2 | Mus musculus (Mouse) | PR |
| P35585 | Ap1m1 | AP-1 complex subunit mu-1 | Mus musculus (Mouse) | PR |
| Q8CDJ8 | Ston1 | Stonin-1 | Mus musculus (Mouse) | PR |
| P35603 | dpy-23 | AP-2 complex subunit mu | Caenorhabditis elegans | PR |
| O23140 | AP2M | AP-2 complex subunit mu | Arabidopsis thaliana (Mouse-ear cress) | PR |
| 10 | 20 | 30 | 40 | 50 | 60 |
| MIGGLFIYNH | KGEVLISRVY | RDDIGRNAVD | AFRVNVIHAR | QQVRSPVTNI | ARTSFFHVKR |
| 70 | 80 | 90 | 100 | 110 | 120 |
| SNIWLAAVTK | QNVNAAMVFE | FLYKMCDVMA | AYFGKISEEN | IKNNFVLIYE | LLDEILDFGY |
| 130 | 140 | 150 | 160 | 170 | 180 |
| PQNSETGALK | TFITQQGIKS | QHQTKEEQSQ | ITSQVTGQIG | WRREGIKYRR | NELFLDVLES |
| 190 | 200 | 210 | 220 | 230 | 240 |
| VNLLMSPQGQ | VLSAHVSGRV | VMKSYLSGMP | ECKFGMNDKI | VIEKQGKGTA | DETSKSGKQS |
| 250 | 260 | 270 | 280 | 290 | 300 |
| IAIDDCTFHQ | CVRLSKFDSE | RSISFIPPDG | EFELMRYRTT | KDIILPFRVI | PLVREVGRTK |
| 310 | 320 | 330 | 340 | 350 | 360 |
| LEVKVVIKSN | FKPSLLAQKI | EVRIPTPLNT | SGVQVICMKG | KAKYKASENA | IVWKIKRMAG |
| 370 | 380 | 390 | 400 | 410 | 420 |
| MKESQISAEI | ELLPTNDKKK | WARPPISMNF | EVPFAPSGLK | VRYLKVFEPK | LNYSDHDVIK |
| 430 | |||||
| WVRYIGRSGI | YETRC |