AiPD: Autoinhibited Protein Database

Gene name	Hars2 (Harsl)
Protein name	Histidine--tRNA ligase, mitochondrial
Names	Histidine--tRNA ligase-like, Histidyl-tRNA synthetase, HisRS
Species	Mus musculus (Mouse)
KEGG Pathway	mmu:70791
EC number	6.1.1.21: Ligases forming aminoacyl-tRNA and related compounds
Protein Class

The autoinhibited protein was predicted that may have potential autoinhibitory elements via cis-regPred.

1-82 (Predicted disordered autoinhibitory region) PR

1-82 (Predicted disordered autoinhibitory region)

cis-regPred

No accessory elements

Entry ID	Method	Resolution	Chain	Position	Source
AF-Q99KK9-F1	Predicted				AlphaFoldDB

Variant ID(s)	Position	Change	Diseaes Association	Provenance
rs252772318	11	A>V	No	EVA
rs3389442722	33	S>I	No	EVA
rs3389491227	60	K>Q	No	EVA
rs3389503463	86	R>G	No	EVA
rs3389475997	97	F>I	No	EVA
rs3389496181	157	R>M	No	EVA
rs3389442655	252	E>K	No	EVA
rs3389475996	267	I>V	No	EVA
rs232437923	270	F>Y	No	EVA
rs3389495803	284	F>Y	No	EVA
rs3389491226	288	R>K	No	EVA
rs258762666	300	G>R	No	EVA
rs3389489403	308	Y>N	No	EVA
rs3389496160	330	Y>H	No	EVA
rs3406542888	333	G>AS*	No	EVA
rs3389499971	436	I>F	No	EVA
rs3389405632	443	K>R	No	EVA
rs3389488505	447	K>R	No	EVA
rs3389489442	454	Y>C	No	EVA
rs3389510723	467	G>C	No	EVA
rs13489285	494	L>F	No	EVA

Type	Name	Position	InterPro Accession
domain	SNF2, N-terminal	243 - 721	IPR000330
domain	Helicase, C-terminal	834 - 996	IPR001650
domain	Zinc finger, RING-type	760 - 801	IPR001841
domain	Helicase superfamily 1/2, ATP-binding domain	236 - 614	IPR014001
domain	HIRAN domain	60 - 154	IPR014905
conserved_site	Zinc finger, RING-type, conserved site	775 - 784	IPR017907

		Description
EC Number	6.1.1.21	Ligases forming aminoacyl-tRNA and related compounds
Subcellular Localization	Mitochondrion
PANTHER Family
PANTHER Subfamily
PANTHER Protein Class
PANTHER Pathway Category	No pathway information available

Name	Definition
cytosol	The part of the cytoplasm that does not contain organelles but which does contain other particulate matter, such as protein complexes.
mitochondrion	A semiautonomous, self replicating organelle that occurs in varying numbers, shapes, and sizes in the cytoplasm of virtually all eukaryotic cells. It is notably the site of tissue respiration.

Name	Definition
ATP binding	Binding to ATP, adenosine 5'-triphosphate, a universally important coenzyme and enzyme regulator.
histidine-tRNA ligase activity	Catalysis of the reaction: ATP + L-histidine + tRNA(His) = AMP + diphosphate + L-histidyl-tRNA(His).
identical protein binding	Binding to an identical protein or proteins.

Name	Definition
histidyl-tRNA aminoacylation	The process of coupling histidine to histidyl-tRNA, catalyzed by histidyl-tRNA synthetase. The histidyl-tRNA synthetase is a class-II synthetase. The activated amino acid is transferred to the 3''-OH group of a histidine-accetping tRNA.

UniProt AC	Gene Name	Protein Name	Species	Evidence Code
Q2KI84	HARS1	Histidine--tRNA ligase, cytoplasmic	Bos taurus (Bovine)	PR
A5D7V9	HARS2	Histidine--tRNA ligase, mitochondrial	Bos taurus (Bovine)	PR
P12081	HARS1	Histidine--tRNA ligase, cytoplasmic	Homo sapiens (Human)	PR
P49590	HARS2	Histidine--tRNA ligase, mitochondrial	Homo sapiens (Human)	PR
Q61035	Hars1	Histidine--tRNA ligase, cytoplasmic	Mus musculus (Mouse)	PR
P93422	Os09g0504400	Histidine--tRNA ligase, cytoplasmic	Oryza sativa subsp japonica (Rice)	PR
P34183	hars-1	Histidine--tRNA ligase	Caenorhabditis elegans	PR
F4IYF8	At3g02760	Histidine--tRNA ligase, cytoplasmic	Arabidopsis thaliana (Mouse-ear cress)	PR
O82413	At3g46100	Histidine--tRNA ligase, chloroplastic/mitochondrial	Arabidopsis thaliana (Mouse-ear cress)	PR