P34183

PR
Gene name
hars-1
Protein name
Histidine--tRNA ligase
Names
Histidyl-tRNA synthetase, HisRS
Species
Caenorhabditis elegans
KEGG Pathway
cel:CELE_T11G6.1
EC number
6.1.1.21: Ligases forming aminoacyl-tRNA and related compounds
Protein Class

Descriptions

The autoinhibited protein was predicted that may have potential autoinhibitory elements via cis-regPred.

Autoinhibitory domains (AIDs)

6-88 (Predicted disordered autoinhibitory region) PR

Target domain

Relief mechanism

Assay

cis-regPred

Accessory elements

No accessory elements

References

Autoinhibited structure

Activated structure

1 structures for P34183

Entry ID Method Resolution Chain Position Source
AF-P34183-F1 Predicted AlphaFoldDB

No variants for P34183

Variant ID(s) Position Change Description Diseaes Association Provenance
No variants for P34183

No associated diseases with P34183

4 regional properties for P34183

Type Name Position InterPro Accession
domain Anticodon-binding 427 - 516 IPR004154
domain Aminoacyl-tRNA synthetase, class II 67 - 445 IPR006195
domain Histidyl-anticodon-binding 424 - 515 IPR033656
domain Class II Histidinyl-tRNA synthetase (HisRS)-like catalytic core domain 67 - 413 IPR041715

Functions

Description
EC Number 6.1.1.21 Ligases forming aminoacyl-tRNA and related compounds
Subcellular Localization
PANTHER Family
PANTHER Subfamily
PANTHER Protein Class
PANTHER Pathway Category No pathway information available

3 GO annotations of cellular component

Name Definition
cytoplasm The contents of a cell excluding the plasma membrane and nucleus, but including other subcellular structures.
cytosol The part of the cytoplasm that does not contain organelles but which does contain other particulate matter, such as protein complexes.
mitochondrion A semiautonomous, self replicating organelle that occurs in varying numbers, shapes, and sizes in the cytoplasm of virtually all eukaryotic cells. It is notably the site of tissue respiration.

3 GO annotations of molecular function

Name Definition
ATP binding Binding to ATP, adenosine 5'-triphosphate, a universally important coenzyme and enzyme regulator.
histidine-tRNA ligase activity Catalysis of the reaction: ATP + L-histidine + tRNA(His) = AMP + diphosphate + L-histidyl-tRNA(His).
identical protein binding Binding to an identical protein or proteins.

5 GO annotations of biological process

Name Definition
germ cell development The process whose specific outcome is the progression of an immature germ cell over time, from its formation to the mature structure (gamete). A germ cell is any reproductive cell in a multicellular organism.
histidyl-tRNA aminoacylation The process of coupling histidine to histidyl-tRNA, catalyzed by histidyl-tRNA synthetase. The histidyl-tRNA synthetase is a class-II synthetase. The activated amino acid is transferred to the 3''-OH group of a histidine-accetping tRNA.
mitochondrial translation The chemical reactions and pathways resulting in the formation of a protein in a mitochondrion. This is a ribosome-mediated process in which the information in messenger RNA (mRNA) is used to specify the sequence of amino acids in the protein; the mitochondrion has its own ribosomes and transfer RNAs, and uses a genetic code that differs from the nuclear code.
nematode larval development The process whose specific outcome is the progression of the nematode larva over time, from its formation to the mature structure. Nematode larval development begins with the newly hatched first-stage larva (L1) and ends with the end of the last larval stage (for example the fourth larval stage (L4) in C. elegans). Each stage of nematode larval development is characterized by proliferation of specific cell lineages and an increase in body size without alteration of the basic body plan. Nematode larval stages are separated by molts in which each stage-specific exoskeleton, or cuticle, is shed and replaced anew.
translation The cellular metabolic process in which a protein is formed, using the sequence of a mature mRNA or circRNA molecule to specify the sequence of amino acids in a polypeptide chain. Translation is mediated by the ribosome, and begins with the formation of a ternary complex between aminoacylated initiator methionine tRNA, GTP, and initiation factor 2, which subsequently associates with the small subunit of the ribosome and an mRNA or circRNA. Translation ends with the release of a polypeptide chain from the ribosome.

9 homologous proteins in AiPD

UniProt AC Gene Name Protein Name Species Evidence Code
Q2KI84 HARS1 Histidine--tRNA ligase, cytoplasmic Bos taurus (Bovine) PR
A5D7V9 HARS2 Histidine--tRNA ligase, mitochondrial Bos taurus (Bovine) PR
P12081 HARS1 Histidine--tRNA ligase, cytoplasmic Homo sapiens (Human) PR
P49590 HARS2 Histidine--tRNA ligase, mitochondrial Homo sapiens (Human) PR
Q99KK9 Hars2 Histidine--tRNA ligase, mitochondrial Mus musculus (Mouse) PR
Q61035 Hars1 Histidine--tRNA ligase, cytoplasmic Mus musculus (Mouse) PR
P93422 Os09g0504400 Histidine--tRNA ligase, cytoplasmic Oryza sativa subsp japonica (Rice) PR
F4IYF8 At3g02760 Histidine--tRNA ligase, cytoplasmic Arabidopsis thaliana (Mouse-ear cress) PR
O82413 At3g46100 Histidine--tRNA ligase, chloroplastic/mitochondrial Arabidopsis thaliana (Mouse-ear cress) PR
10 20 30 40 50 60
MFRTITSRLA FSVRQAEETV KPRIAYAQRS GRRIKEKVAL MQAKRKEAGE TGAPEKPGKF
70 80 90 100 110 120
VLKTGKGTRD YGPAQSALRN SVLQTVTETF NRYGAETIDT PVFELRDVLM GKYGEEGGKL
130 140 150 160 170 180
VYDLQDQGGE LLSLRYDLTV PFARYLAMNK ITNITRYQIA KVYRRDQPVM SRGRYREFYQ
190 200 210 220 230 240
CDFDIAGQYD LMLPEAECLG IVDELLTKLE IGEFFINLNH RLILEGMFAV SGIPAKDFKT
250 260 270 280 290 300
ICSSVDKLDK TPWEDVEQEM INEKFLTKEQ TGKLGELVRF RELNSDLNNL ELLEKMSQLP
310 320 330 340 350 360
DLGQNDKFKK GAEELKVLIE YLNVDGVTTV RYEPSLARGL DYYTGAIYEA VAPKALEGTA
370 380 390 400 410 420
VENSEDTAGQ PVGVGSVAAG GRYDGLVKMF DSKANVPCCG VSFGIERLFA IMEARQKVAI
430 440 450 460 470 480
RTTQTEVYVA SAQKNLVRDR KKLVKMLRSA GIKTEMALKA NPKLLTQFQY AEERRIPLAI
490 500 510 520
VIGEQELKDG VVKLRNVVTR DEQTIKLDQL ITAVRDTLAA L