Q9M2A0

SS
Gene name
AHA8 (At3g42640, T12K4_90)
Protein name
ATPase 8, plasma membrane-type
Names
EC 7.1.2.1 , Proton pump 8
Species
Arabidopsis thaliana (Mouse-ear cress)
KEGG Pathway
ath:AT3G42640
EC number
7.1.2.1: Hydron translocation linked to the hydrolysis of a nucleoside triphosphate
Protein Class

Descriptions

The plasma membrane H+-ATPase belongs to the superfamily of P-type ATPases. The H+-ATPase is responsible for the ATP-fuelled ejection of protons from the cell and is an important component in the generation of the trans-membrane H+ gradient in plant cells. The H+-ATPase AHA2 is controlled by an autoinhibitory C-terminal regulatory domain and activated by 14-3-3 proteins which bind to the part of the enzyme. In two regions of the C-terminus, alteration of the residues leads to increased pump activity. One region stretches from K863 to L885 and includes two residues (Q879 and R880) that are conserved between plant and fungal H+-ATPases. The other region, incorporating S904 to L919, is situated in an extension of the C-terminus unique to plant H+-ATPases.

Autoinhibitory domains (AIDs)

866-888 (Autoinhibitory domain I) SS

Target domain

312-540 (ATPase domain)

Relief mechanism

Partner binding

Assay

903-918 (Autoinhibitory domain II) SS

Target domain

312-540 (ATPase domain)

Relief mechanism

Partner binding

Assay

Accessory elements

No accessory elements

References

Autoinhibited structure

Activated structure

1 structures for Q9M2A0

Entry ID Method Resolution Chain Position Source
AF-Q9M2A0-F1 Predicted AlphaFoldDB

86 variants for Q9M2A0

Variant ID(s) Position Change Description Diseaes Association Provenance
tmp_3_14724325_C_T 6 S>L No 1000Genomes
ENSVATH12070580 13 E>D No 1000Genomes
tmp_3_14724355_A_T 16 D>V No 1000Genomes
tmp_3_14724460_G_A 23 E>K No 1000Genomes
tmp_3_14724465_A_C 24 E>D No 1000Genomes
tmp_3_14724475_C_G 28 Q>E No 1000Genomes
ENSVATH02359141 34 E>Q No 1000Genomes
ENSVATH00384393 45 L>R No 1000Genomes
tmp_3_14724581_A_G 63 K>R No 1000Genomes
tmp_3_14724595_A_G 68 M>V No 1000Genomes
ENSVATH12070583 93 A>T No 1000Genomes
ENSVATH12070626 140 D>N No 1000Genomes
tmp_3_14724903_G_A 144 G>E No 1000Genomes
tmp_3_14724909_A_C 146 Q>P No 1000Genomes
ENSVATH14261221 157 I>V No 1000Genomes
ENSVATH06126558 160 K>N No 1000Genomes
tmp_3_14724989_G_A 173 E>K No 1000Genomes
tmp_3_14725141_T_G 223 F>L No 1000Genomes
ENSVATH00384395 231 D>E No 1000Genomes
ENSVATH12070627 234 N>S No 1000Genomes
ENSVATH12070628 236 V>F No 1000Genomes
tmp_3_14725276_G_A 242 V>I No 1000Genomes
ENSVATH06126563 250 C>S No 1000Genomes
tmp_3_14725444_G_A 298 V>I No 1000Genomes
tmp_3_14725465_C_T 305 H>Y No 1000Genomes
ENSVATH00384396 349 I>V No 1000Genomes
ENSVATH06126566 350 E>D No 1000Genomes
ENSVATH02359146 354 K>Q No 1000Genomes
ENSVATH02359147 362 V>L No 1000Genomes
ENSVATH14261235 366 A>V No 1000Genomes
ENSVATH00384397 371 I>M No 1000Genomes
tmp_3_14725677_T_A 375 D>E No 1000Genomes
ENSVATH06126568 384 M>I No 1000Genomes
tmp_3_14725708_G_A 386 G>S No 1000Genomes
ENSVATH00384400 401 L>S No 1000Genomes
ENSVATH02359149 409 R>C No 1000Genomes
ENSVATH06126573 442 T>N No 1000Genomes
tmp_3_14726033_A_C 442 T>P No 1000Genomes
tmp_3_14726051_G_A 448 E>K No 1000Genomes
ENSVATH02359151 449 V>I No 1000Genomes
ENSVATH00384401 450 I>V No 1000Genomes
tmp_3_14726060_G_A 451 D>N No 1000Genomes
ENSVATH02359152 452 G>C No 1000Genomes
tmp_3_14726099_G_A 464 A>T No 1000Genomes
ENSVATH06126579 475 S>N No 1000Genomes
tmp_3_14726228_A_C 478 S>R No 1000Genomes
tmp_3_14726243_G_T 483 V>F No 1000Genomes
tmp_3_14726261_T_C 489 F>L No 1000Genomes
tmp_3_14726315_G_C 507 G>R No 1000Genomes
ENSVATH12070668 510 V>I No 1000Genomes
tmp_3_14726349_T_A,C 518 L>H No 1000Genomes
tmp_3_14726349_T_A,C 518 L>P No 1000Genomes
tmp_3_14726426_T_G 544 S>A No 1000Genomes
ENSVATH02359158 547 E>Q No 1000Genomes
tmp_3_14726444_G_A 550 V>I No 1000Genomes
tmp_3_14726634_A_T 581 E>V No 1000Genomes
tmp_3_14726720_G_A 610 D>N No 1000Genomes
tmp_3_14726786_A_G 632 I>V No 1000Genomes
tmp_3_14726810_G_T 640 A>S No 1000Genomes
ENSVATH06126587 657 I>L No 1000Genomes
tmp_3_14726936_A_C 682 I>L No 1000Genomes
tmp_3_14726939_G_T 683 A>S No 1000Genomes
tmp_3_14726969_A_G 693 I>V No 1000Genomes
tmp_3_14726977_G_C 695 K>N No 1000Genomes
tmp_3_14726988_A_T 699 K>I No 1000Genomes
ENSVATH06126589 700 P>S No 1000Genomes
ENSVATH14261238 710 N>Y No 1000Genomes
tmp_3_14727032_G_A 714 A>T No 1000Genomes
tmp_3_14727059_A_G 723 M>V No 1000Genomes
tmp_3_14727069_C_T 726 T>I No 1000Genomes
tmp_3_14727196_G_C 746 V>L No 1000Genomes
ENSVATH12070698 747 R>M No 1000Genomes
ENSVATH06126593 753 E>A No 1000Genomes
ENSVATH06126594 755 E>D No 1000Genomes
ENSVATH02359162 758 A>G No 1000Genomes
tmp_3_14727495_G_A 817 G>S No 1000Genomes
ENSVATH00384408 825 G>V No 1000Genomes
tmp_3_14727540_A_G 832 I>V No 1000Genomes
tmp_3_14727551_T_G 835 I>M No 1000Genomes
tmp_3_14727717_A_G 865 T>A No 1000Genomes
tmp_3_14727721_A_G 866 K>R No 1000Genomes
tmp_3_14727739_G_A 872 G>E No 1000Genomes
tmp_3_14727825_G_A 901 E>K No 1000Genomes
tmp_3_14727874_G_A 917 R>K No 1000Genomes
ENSVATH06126612 939 I>V No 1000Genomes
tmp_3_14728034_A_G 940 D>G No 1000Genomes

No associated diseases with Q9M2A0

3 regional properties for Q9M2A0

Type Name Position InterPro Accession
domain Cation-transporting P-type ATPase, N-terminal 16 - 88 IPR004014
ptm P-type ATPase, phosphorylation site 332 - 338 IPR018303
domain P-type ATPase, haloacid dehalogenase domain 312 - 640 IPR044492

Functions

Description
EC Number 7.1.2.1 Hydron translocation linked to the hydrolysis of a nucleoside triphosphate
Subcellular Localization
  • Membrane; Multi-pass membrane protein
PANTHER Family
PANTHER Subfamily
PANTHER Protein Class
PANTHER Pathway Category No pathway information available

1 GO annotations of cellular component

Name Definition
plasma membrane The membrane surrounding a cell that separates the cell from its external environment. It consists of a phospholipid bilayer and associated proteins.

4 GO annotations of molecular function

Name Definition
ATP binding Binding to ATP, adenosine 5'-triphosphate, a universally important coenzyme and enzyme regulator.
ATP hydrolysis activity Catalysis of the reaction
metal ion binding Binding to a metal ion.
P-type proton-exporting transporter activity Enables the transfer of protons from one side of a membrane to the other according to the reaction

2 GO annotations of biological process

Name Definition
proton export across plasma membrane The directed movement of hydrogen ions (protons) from inside a cell, across the plasma membrane and into the extracellular region.
regulation of intracellular pH Any process that modulates the internal pH of a cell, measured by the concentration of the hydrogen ion.

15 homologous proteins in AiPD

UniProt AC Gene Name Protein Name Species Evidence Code
P83970 ha1 Plasma membrane ATPase Triticum aestivum (Wheat) SS
Q80XR2 Atp2c1 Calcium-transporting ATPase type 2C member 1 Mus musculus (Mouse) PR
A7L9Z8 Atp2c2 Calcium-transporting ATPase type 2C member 2 Mus musculus (Mouse) PR
Q7XPY2 Os04g0656100 Plasma membrane ATPase Oryza sativa subsp. japonica (Rice) SS
P20431 AHA3 ATPase 3, plasma membrane-type Arabidopsis thaliana (Mouse-ear cress) SS
P20649 AHA1 ATPase 1, plasma membrane-type Arabidopsis thaliana (Mouse-ear cress) SS
Q43128 AHA10 ATPase 10, plasma membrane-type Arabidopsis thaliana (Mouse-ear cress) SS
Q9LV11 AHA11 ATPase 11, plasma membrane-type Arabidopsis thaliana (Mouse-ear cress) SS
Q9LY32 AHA7 ATPase 7, plasma membrane-type Arabidopsis thaliana (Mouse-ear cress) SS
Q9SH76 AHA6 ATPase 6, plasma membrane-type Arabidopsis thaliana (Mouse-ear cress) SS
Q9SJB3 AHA5 ATPase 5, plasma membrane-type Arabidopsis thaliana (Mouse-ear cress) SS
Q9SU58 AHA4 ATPase 4, plasma membrane-type Arabidopsis thaliana (Mouse-ear cress) SS
P19456 AHA2 ATPase 2, plasma membrane-type Arabidopsis thaliana (Mouse-ear cress) EV
Q42556 AHA9 ATPase 9, plasma membrane-type Arabidopsis thaliana (Mouse-ear cress) SS
P22180 LHA1 Plasma membrane ATPase 1 Solanum lycopersicum (Tomato) (Lycopersicon esculentum) SS
10 20 30 40 50 60
MATEFSWDEI KKENVDLERI PVEEVFEQLK CSKEGLSSDE GAKRLEIFGA NKLEEKSENK
70 80 90 100 110 120
FLKFLGFMWN PLSWVMESAA IMAIVLANGG GKAPDWQDFI GIMVLLIINS TISFIEENNA
130 140 150 160 170 180
GNAAAALMAN LAPKTKVLRD GKWGEQEASI LVPGDLISIK LGDIVPADAR LLEGDPLKID
190 200 210 220 230 240
QSALTGESLP TTKHPGDEVF SGSTCKQGEI EAVVIATGVH TFFGKAAHLV DSTNNVGHFQ
250 260 270 280 290 300
KVLTSIGNFC ICSIGLGMLI EILIMYPIQH RTYRDGIDNL LVLLIGGIPI AMPTVLSVTM
310 320 330 340 350 360
AIGSHRLSQQ GAITKRMTAI EEMAGMDVLC SDKTGTLTLN KLSVDKSLIE VFPKNMDSDS
370 380 390 400 410 420
VVLMAARASR IENQDAIDAS IVGMLGDPKE ARAGITEVHF LPFNPVDKRT AITYIDESGD
430 440 450 460 470 480
WHRSSKGAPE QIIELCNLQG ETKRKAHEVI DGFAERGLRS LGVAQQTVPE KTKESDGSPW
490 500 510 520 530 540
EFVGLLPLFD PPRHDSAETI RRALELGVNV KMITGDQLAI GIETGRRLGM GTNMYPSTSL
550 560 570 580 590 600
LGNSKDESLV GIPIDELIEK ADGFAGVFPE HKYEIVKKLQ ERKHICGMTG DGVNDAPALK
610 620 630 640 650 660
KADIGIAVAD ATDAARSASD IVLTEPGLSV IISAVLTSRA IFQRMKNYTI YAVSITIRIV
670 680 690 700 710 720
LGFMLVALIW RFDFAPFMVL IIAILNDGTI MTISKDRVKP SPVPDSWKLN EIFATGVVLG
730 740 750 760 770 780
TYMALTTVLF FWLAHDTDFF SKTFGVRSIQ GNEEELMAAL YLQVSIISQA LIFVTRSRSW
790 800 810 820 830 840
SFVERPGFLL LIAFVIAQLV ATLIAVYANW GFARIVGCGW GWAGGIWVYS IITYIPLDIL
850 860 870 880 890 900
KFIIRYALTG KAWDNMINQK TAFTTKKDYG KGEREAQWAL AQRTLHGLPP PEAMFNDNKN
910 920 930 940
ELSEIAEQAK RRAEVARLRE LHTLKGHVES VVKLKGLDID TIQQHYTV