P22180

SS
Gene name
LHA1
Protein name
Plasma membrane ATPase 1
Names
EC 7.1.2.1 , Proton pump 1
Species
Rattus norvegicus (Rat)
KEGG Pathway
sly:543982
EC number
Protein Class

Descriptions

Small GTPases couple their GDP/GTP structural cycle to cytosol/membrane alternation to function as versatile molecular switches in the cell. Membrane localization of their active, GTP-bound form is pivotal to their ability to propagate information, and this requires their post-translational modification by lipids.<br>Arf GTPases are modified by a myristate attached to their N-terminus, which is shielded by intramolecular interactions in their inactive state. The myristoylated N-terminus of Arf is autoinhibitory in solution and is displaced by membranes, priming Arf GTPases for activation by their GEFs. Replacement of the N-terminal myristate by a 6xHis-tag preserves autoinhibition, representing that membranes unlock the N-terminal region to facilitate subsequent activation.

Autoinhibitory domains (AIDs)

871-893 (Autoinhibitory domain I) SS

Target domain

313-541 (ATPase domain)

Relief mechanism

Partner binding

Assay

911-926 (Autoinhibitory domain II) SS

Target domain

313-541 (ATPase domain)

Relief mechanism

Partner binding

Assay

Accessory elements

No accessory elements

References

Autoinhibited structure

Activated structure

1 structures for P22180

Entry ID Method Resolution Chain Position Source
AF-P22180-F1 Predicted AlphaFoldDB

No variants for P22180

Variant ID(s) Position Change Description Diseaes Association Provenance
No variants for P22180

No associated diseases with P22180

1 regional properties for P22180

Type Name Position InterPro Accession
domain Small GTP-binding protein domain 12 - 139 IPR005225

Functions

Description
EC Number
Subcellular Localization
  • Golgi apparatus
  • Cytoplasm, perinuclear region
PANTHER Family
PANTHER Subfamily
PANTHER Protein Class
PANTHER Pathway Category No pathway information available

1 GO annotations of cellular component

Name Definition
plasma membrane The membrane surrounding a cell that separates the cell from its external environment. It consists of a phospholipid bilayer and associated proteins.

4 GO annotations of molecular function

Name Definition
ATP binding Binding to ATP, adenosine 5'-triphosphate, a universally important coenzyme and enzyme regulator.
ATP hydrolysis activity Catalysis of the reaction
metal ion binding Binding to a metal ion.
P-type proton-exporting transporter activity Enables the transfer of protons from one side of a membrane to the other according to the reaction

3 GO annotations of biological process

Name Definition
proton export across plasma membrane The directed movement of hydrogen ions (protons) from inside a cell, across the plasma membrane and into the extracellular region.
proton transmembrane transport The directed movement of a proton across a membrane.
regulation of intracellular pH Any process that modulates the internal pH of a cell, measured by the concentration of the hydrogen ion.

13 homologous proteins in AiPD

UniProt AC Gene Name Protein Name Species Evidence Code
P83970 ha1 Plasma membrane ATPase Triticum aestivum (Wheat) SS
Q7XPY2 Os04g0656100 Plasma membrane ATPase Oryza sativa subsp. japonica (Rice) SS
Q43128 AHA10 ATPase 10, plasma membrane-type Arabidopsis thaliana (Mouse-ear cress) SS
Q42556 AHA9 ATPase 9, plasma membrane-type Arabidopsis thaliana (Mouse-ear cress) SS
Q9SH76 AHA6 ATPase 6, plasma membrane-type Arabidopsis thaliana (Mouse-ear cress) SS
P20649 AHA1 ATPase 1, plasma membrane-type Arabidopsis thaliana (Mouse-ear cress) SS
Q9SJB3 AHA5 ATPase 5, plasma membrane-type Arabidopsis thaliana (Mouse-ear cress) SS
Q9M2A0 AHA8 ATPase 8, plasma membrane-type Arabidopsis thaliana (Mouse-ear cress) SS
Q9LY32 AHA7 ATPase 7, plasma membrane-type Arabidopsis thaliana (Mouse-ear cress) SS
P19456 AHA2 ATPase 2, plasma membrane-type Arabidopsis thaliana (Mouse-ear cress) EV
P20431 AHA3 ATPase 3, plasma membrane-type Arabidopsis thaliana (Mouse-ear cress) SS
Q9LV11 AHA11 ATPase 11, plasma membrane-type Arabidopsis thaliana (Mouse-ear cress) SS
Q9SU58 AHA4 ATPase 4, plasma membrane-type Arabidopsis thaliana (Mouse-ear cress) SS
10 20 30 40 50 60
MGNIFGNLLK SLIGKKEMRI LMVGLDAAGK TTILYKLKLG EIVTTIPTIG FNVETVEYKN
70 80 90 100 110 120
ISFTVWDVGG QDKIRPLWRH YFQNTQGLIF VVDSNDRERV NEAREELMRM LAEDELRDAV
130 140 150 160 170 180
LLVFANKQDL PNAMNAAEIT DKLGLHSLRH RNWYIQATCA TSGDGLYEGL DWLANQLKNK
K