P20431

SS
Gene name
AHA3 (At5g57350, MJB24.16)
Protein name
ATPase 3, plasma membrane-type
Names
EC 7.1.2.1 , Proton pump 3
Species
Arabidopsis thaliana (Mouse-ear cress)
KEGG Pathway
ath:AT5G57350
EC number
7.1.2.1: Hydron translocation linked to the hydrolysis of a nucleoside triphosphate
Protein Class

Descriptions

The plasma membrane H+-ATPase belongs to the superfamily of P-type ATPases. The H+-ATPase is responsible for the ATP-fuelled ejection of protons from the cell and is an important component in the generation of the trans-membrane H+ gradient in plant cells. The H+-ATPase AHA2 is controlled by an autoinhibitory C-terminal regulatory domain and activated by 14-3-3 proteins which bind to the part of the enzyme. In two regions of the C-terminus, alteration of the residues leads to increased pump activity. One region stretches from K863 to L885 and includes two residues (Q879 and R880) that are conserved between plant and fungal H+-ATPases. The other region, incorporating S904 to L919, is situated in an extension of the C-terminus unique to plant H+-ATPases.

Autoinhibitory domains (AIDs)

864-886 (Autoinhibitory domain I) SS

Target domain

310-538 (ATPase domain)

Relief mechanism

Partner binding

Assay

905-920 (Autoinhibitory domain II) SS

Target domain

310-538 (ATPase domain)

Relief mechanism

Partner binding

Assay

Accessory elements

No accessory elements

References

Autoinhibited structure

Activated structure

1 structures for P20431

Entry ID Method Resolution Chain Position Source
AF-P20431-F1 Predicted AlphaFoldDB

17 variants for P20431

Variant ID(s) Position Change Description Diseaes Association Provenance
tmp_5_23235319_T_A 18 I>L No 1000Genomes
tmp_5_23235264_C_T 36 G>E No 1000Genomes
ENSVATH07439614 37 A>V No 1000Genomes
ENSVATH03443980 113 V>I No 1000Genomes
ENSVATH14637505 192 G>A No 1000Genomes
tmp_5_23233981_C_A 363 A>S No 1000Genomes
ENSVATH14637503 369 V>I No 1000Genomes
tmp_5_23233390_C_T 466 V>I No 1000Genomes
ENSVATH03443972 472 E>D No 1000Genomes
ENSVATH03443971 475 G>S No 1000Genomes
ENSVATH03443970 476 S>C No 1000Genomes
ENSVATH12826107 557 E>V No 1000Genomes
ENSVATH03443963 732 A>T No 1000Genomes
ENSVATH03443961 790 I>F No 1000Genomes
ENSVATH03443959 798 I>F No 1000Genomes
tmp_5_23231810_G_C 801 A>G No 1000Genomes
tmp_5_23231786_T_C 809 E>G No 1000Genomes

No associated diseases with P20431

6 regional properties for P20431

Type Name Position InterPro Accession
domain Protein kinase domain 98 - 366 IPR000719
domain Serine-threonine/tyrosine-protein kinase, catalytic domain 98 - 357 IPR001245
domain SH3 domain 16 - 81 IPR001452
active_site Serine/threonine-protein kinase, active site 218 - 230 IPR008271
binding_site Protein kinase, ATP binding site 104 - 125 IPR017441
domain MLK1-3, SH3 domain 20 - 76 IPR035779

Functions

Description
EC Number 7.1.2.1 Hydron translocation linked to the hydrolysis of a nucleoside triphosphate
Subcellular Localization
  • Cell membrane ; Multi-pass membrane protein
PANTHER Family
PANTHER Subfamily
PANTHER Protein Class
PANTHER Pathway Category No pathway information available

4 GO annotations of cellular component

Name Definition
membrane A lipid bilayer along with all the proteins and protein complexes embedded in it and attached to it.
plasma membrane The membrane surrounding a cell that separates the cell from its external environment. It consists of a phospholipid bilayer and associated proteins.
plasmodesma A fine cytoplasmic channel, found in all higher plants, that connects the cytoplasm of one cell to that of an adjacent cell.
plastid Any member of a family of organelles found in the cytoplasm of plants and some protists, which are membrane-bounded and contain DNA. Plant plastids develop from a common type, the proplastid.

4 GO annotations of molecular function

Name Definition
ATP binding Binding to ATP, adenosine 5'-triphosphate, a universally important coenzyme and enzyme regulator.
ATP hydrolysis activity Catalysis of the reaction
magnesium ion binding Binding to a magnesium (Mg) ion.
P-type proton-exporting transporter activity Enables the transfer of protons from one side of a membrane to the other according to the reaction

3 GO annotations of biological process

Name Definition
proton export across plasma membrane The directed movement of hydrogen ions (protons) from inside a cell, across the plasma membrane and into the extracellular region.
proton transmembrane transport The directed movement of a proton across a membrane.
regulation of intracellular pH Any process that modulates the internal pH of a cell, measured by the concentration of the hydrogen ion.

15 homologous proteins in AiPD

UniProt AC Gene Name Protein Name Species Evidence Code
P83970 ha1 Plasma membrane ATPase Triticum aestivum (Wheat) SS
A7L9Z8 Atp2c2 Calcium-transporting ATPase type 2C member 2 Mus musculus (Mouse) PR
Q80XR2 Atp2c1 Calcium-transporting ATPase type 2C member 1 Mus musculus (Mouse) PR
Q7XPY2 Os04g0656100 Plasma membrane ATPase Oryza sativa subsp. japonica (Rice) SS
P20649 AHA1 ATPase 1, plasma membrane-type Arabidopsis thaliana (Mouse-ear cress) SS
Q43128 AHA10 ATPase 10, plasma membrane-type Arabidopsis thaliana (Mouse-ear cress) SS
Q9LV11 AHA11 ATPase 11, plasma membrane-type Arabidopsis thaliana (Mouse-ear cress) SS
Q9LY32 AHA7 ATPase 7, plasma membrane-type Arabidopsis thaliana (Mouse-ear cress) SS
Q9M2A0 AHA8 ATPase 8, plasma membrane-type Arabidopsis thaliana (Mouse-ear cress) SS
Q9SH76 AHA6 ATPase 6, plasma membrane-type Arabidopsis thaliana (Mouse-ear cress) SS
Q9SJB3 AHA5 ATPase 5, plasma membrane-type Arabidopsis thaliana (Mouse-ear cress) SS
Q9SU58 AHA4 ATPase 4, plasma membrane-type Arabidopsis thaliana (Mouse-ear cress) SS
P19456 AHA2 ATPase 2, plasma membrane-type Arabidopsis thaliana (Mouse-ear cress) EV
Q42556 AHA9 ATPase 9, plasma membrane-type Arabidopsis thaliana (Mouse-ear cress) SS
P22180 LHA1 Plasma membrane ATPase 1 Solanum lycopersicum (Tomato) (Lycopersicon esculentum) SS
10 20 30 40 50 60
MASGLEDIVN ENVDLEKIPI EEVFQQLKCS REGLSGAEGE NRLQIFGPNK LEEKKESKLL
70 80 90 100 110 120
KFLGFMWNPL SWVMEAAAIM AIALANGGGK PPDWQDFVGI VCLLVINSTI SFVEENNAGN
130 140 150 160 170 180
AAAALMAGLA PKTKVLRDGK WSEQEASILV PGDIVSIKLG DIIPADARLL EGDPLKVDQS
190 200 210 220 230 240
ALTGESLPAT KGPGEEVFSG STCKQGEIEA VVIATGVHTF FGKAAHLVDS TNQVGHFQKV
250 260 270 280 290 300
LTAIGNFCIC SIAVGIAIEI VVMYPIQRRH YRDGIDNLLV LLIGGIPIAM PTVLSVTMAI
310 320 330 340 350 360
GSHKLSQQGA ITKRMTAIEE MAGMDVLCSD KTGTLTLNKL SVDKNLIEVY CKGVEKDEVL
370 380 390 400 410 420
LFAARASRVE NQDAIDAAMV GMLADPKEAR AGIREIHFLP FNPVDKRTAL TFIDSNGNWH
430 440 450 460 470 480
RVSKGAPEQI LDLCNARADL RKRVHSTIDK YAERGLRSLA VSRQTVPEKT KESSGSPWEF
490 500 510 520 530 540
VGVLPLFDPP RHDSAETIRR ALDLGVNVKM ITGDQLAIAK ETGRRLGMGS NMYPSSSLLG
550 560 570 580 590 600
KHKDEAMAHI PVEDLIEKAD GFAGVFPEHK YEIVKKLQER KHICGMTGDG VNDAPALKKA
610 620 630 640 650 660
DIGIAVADAT DAARGASDIV LTEPGLSVII SAVLTSRAIF QRMKNYTIYA VSITIRIVFG
670 680 690 700 710 720
FMLIALIWKF DFSPFMVLII AILNDGTIMT ISKDRVKPSP TPDSWKLKEI FATGVVLGGY
730 740 750 760 770 780
MAIMTVVFFW AAYKTDFFPR TFHVRDLRGS EHEMMSALYL QVSIVSQALI FVTRSRSWSF
790 800 810 820 830 840
TERPGYFLLI AFWVAQLIAT AIAVYGNWEF ARIKGIGWGW AGVIWLYSIV FYFPLDIMKF
850 860 870 880 890 900
AIRYILAGTA WKNIIDNRTA FTTKQNYGIE EREAQWAHAQ RTLHGLQNTE TANVVPERGG
910 920 930 940
YRELSEIANQ AKRRAEIARL RELHTLKGHV ESVVKLKGLD IETAGHYTV