Q9LY32

SS
Gene name
AHA7 (At3g60330, F27H5_120)
Protein name
ATPase 7, plasma membrane-type
Names
EC 7.1.2.1 , Proton pump 7
Species
Arabidopsis thaliana (Mouse-ear cress)
KEGG Pathway
ath:AT3G60330
EC number
7.1.2.1: Hydron translocation linked to the hydrolysis of a nucleoside triphosphate
Protein Class

Descriptions

The plasma membrane H+-ATPase belongs to the superfamily of P-type ATPases. The H+-ATPase is responsible for the ATP-fuelled ejection of protons from the cell and is an important component in the generation of the trans-membrane H+ gradient in plant cells. The H+-ATPase AHA2 is controlled by an autoinhibitory C-terminal regulatory domain and activated by 14-3-3 proteins which bind to the part of the enzyme. In two regions of the C-terminus, alteration of the residues leads to increased pump activity. One region stretches from K863 to L885 and includes two residues (Q879 and R880) that are conserved between plant and fungal H+-ATPases. The other region, incorporating S904 to L919, is situated in an extension of the C-terminus unique to plant H+-ATPases.

Autoinhibitory domains (AIDs)

876-898 (Autoinhibitory domain I) SS

Target domain

312-540 (ATPase domain)

Relief mechanism

Partner binding

Assay

915-930 (Autoinhibitory domain II) SS

Target domain

312-540 (ATPase domain)

Relief mechanism

Partner binding

Assay

Accessory elements

No accessory elements

References

Autoinhibited structure

Activated structure

1 structures for Q9LY32

Entry ID Method Resolution Chain Position Source
AF-Q9LY32-F1 Predicted AlphaFoldDB

18 variants for Q9LY32

Variant ID(s) Position Change Description Diseaes Association Provenance
ENSVATH06348477 20 V>A No 1000Genomes
ENSVATH06348478 21 P>L No 1000Genomes
tmp_3_22299872_G_T 206 K>N No 1000Genomes
ENSVATH06348491 347 N>I No 1000Genomes
ENSVATH06348492 357 D>N No 1000Genomes
ENSVATH14459972 389 K>N No 1000Genomes
ENSVATH06348493 393 A>V No 1000Genomes
tmp_3_22301266_C_T 487 P>S No 1000Genomes
ENSVATH12848877 542 S>F No 1000Genomes
ENSVATH14459974 556 E>Q No 1000Genomes
ENSVATH02529654 752 K>E No 1000Genomes
ENSVATH02529654 752 K>Q No 1000Genomes
ENSVATH02529655 754 K>N No 1000Genomes
ENSVATH06348497 755 D>N No 1000Genomes
tmp_3_22303240_C_A 902 Q>K No 1000Genomes
ENSVATH06348501 910 S>G No 1000Genomes
tmp_3_22303268_C_G 911 A>G No 1000Genomes
tmp_3_22303472_A_C 950 D>A No 1000Genomes

No associated diseases with Q9LY32

14 regional properties for Q9LY32

Type Name Position InterPro Accession
domain Leucine-rich repeat N-terminal domain 31 - 63 IPR000372
domain Cysteine-rich flanking region, C-terminal 160 - 208 IPR000483
domain Protein kinase domain 538 - 814 IPR000719
domain Serine-threonine/tyrosine-protein kinase, catalytic domain 539 - 809 IPR001245
repeat Leucine-rich repeat 104 - 160 IPR001611
conserved_site Tyrosine-protein kinase, receptor class II, conserved site 703 - 711 IPR002011
domain Immunoglobulin subtype 216 - 302 IPR003599
domain Immunoglobulin-like domain 210 - 300 IPR007110
active_site Tyrosine-protein kinase, active site 675 - 687 IPR008266
domain Immunoglobulin I-set 323 - 391 IPR013098
domain Immunoglobulin-like beta-sandwich domain 218 - 296 IPR013151
binding_site Protein kinase, ATP binding site 544 - 572 IPR017441
domain Tyrosine-protein kinase, catalytic domain 538 - 810 IPR020635
domain Growth factor receptor NTRK, leucine rich repeat C-terminal 163 - 208 IPR031635

Functions

Description
EC Number 7.1.2.1 Hydron translocation linked to the hydrolysis of a nucleoside triphosphate
Subcellular Localization
  • Membrane; Multi-pass membrane protein
PANTHER Family
PANTHER Subfamily
PANTHER Protein Class
PANTHER Pathway Category No pathway information available

1 GO annotations of cellular component

Name Definition
plasma membrane The membrane surrounding a cell that separates the cell from its external environment. It consists of a phospholipid bilayer and associated proteins.

4 GO annotations of molecular function

Name Definition
ATP binding Binding to ATP, adenosine 5'-triphosphate, a universally important coenzyme and enzyme regulator.
ATP hydrolysis activity Catalysis of the reaction
metal ion binding Binding to a metal ion.
P-type proton-exporting transporter activity Enables the transfer of protons from one side of a membrane to the other according to the reaction

2 GO annotations of biological process

Name Definition
proton export across plasma membrane The directed movement of hydrogen ions (protons) from inside a cell, across the plasma membrane and into the extracellular region.
regulation of intracellular pH Any process that modulates the internal pH of a cell, measured by the concentration of the hydrogen ion.

15 homologous proteins in AiPD

UniProt AC Gene Name Protein Name Species Evidence Code
P83970 ha1 Plasma membrane ATPase Triticum aestivum (Wheat) SS
A7L9Z8 Atp2c2 Calcium-transporting ATPase type 2C member 2 Mus musculus (Mouse) PR
Q80XR2 Atp2c1 Calcium-transporting ATPase type 2C member 1 Mus musculus (Mouse) PR
Q7XPY2 Os04g0656100 Plasma membrane ATPase Oryza sativa subsp. japonica (Rice) SS
P20431 AHA3 ATPase 3, plasma membrane-type Arabidopsis thaliana (Mouse-ear cress) SS
P20649 AHA1 ATPase 1, plasma membrane-type Arabidopsis thaliana (Mouse-ear cress) SS
Q43128 AHA10 ATPase 10, plasma membrane-type Arabidopsis thaliana (Mouse-ear cress) SS
Q9LV11 AHA11 ATPase 11, plasma membrane-type Arabidopsis thaliana (Mouse-ear cress) SS
Q9M2A0 AHA8 ATPase 8, plasma membrane-type Arabidopsis thaliana (Mouse-ear cress) SS
Q9SH76 AHA6 ATPase 6, plasma membrane-type Arabidopsis thaliana (Mouse-ear cress) SS
Q9SJB3 AHA5 ATPase 5, plasma membrane-type Arabidopsis thaliana (Mouse-ear cress) SS
Q9SU58 AHA4 ATPase 4, plasma membrane-type Arabidopsis thaliana (Mouse-ear cress) SS
P19456 AHA2 ATPase 2, plasma membrane-type Arabidopsis thaliana (Mouse-ear cress) EV
Q42556 AHA9 ATPase 9, plasma membrane-type Arabidopsis thaliana (Mouse-ear cress) SS
P22180 LHA1 Plasma membrane ATPase 1 Solanum lycopersicum (Tomato) (Lycopersicon esculentum) SS
10 20 30 40 50 60
MTDIEALKAI TTESIDLENV PVEEVFQHLK CTKEGLTSNE VQERLTLFGY NKLEEKKESK
70 80 90 100 110 120
ILKFLGFMWN PLSWVMEAAA LMAIGLAHGG GKPADYHDFV GIVVLLLINS TISFVEENNA
130 140 150 160 170 180
GNAAAALMAQ LAPKAKAVRD GKWNEIDAAE LVPGDIVSIK LGDIIPADAR LLEGDPLKID
190 200 210 220 230 240
QATLTGESLP VTKNPGASVY SGSTCKQGEI EAVVIATGVH TFFGKAAHLV DSTTHVGHFQ
250 260 270 280 290 300
KVLTAIGNFC ICSIAVGMAI EIVVIYGLQK RGYRVGIDNL LVLLIGGIPI AMPTVLSVTM
310 320 330 340 350 360
AIGAHRLAQQ GAITKRMTAI EEMAGMDVLC SDKTGTLTLN KLSVDKNLIE VFKRGIDRDM
370 380 390 400 410 420
AVLMAARAAR LENQDAIDTA IVSMLSDPKE ARAGIKELHF LPFSPANRRT ALTYLDGEGK
430 440 450 460 470 480
MHRVSKGAPE EILDMAHNKL EIKEKVHATI DKFAERGLRS LGLAYQEVPD GDVKGEGGPW
490 500 510 520 530 540
DFVALLPLFD PPRHDSAQTI ERALHLGVSV KMITGDQLAI AKETGRRLGM GTNMYPSSSL
550 560 570 580 590 600
LSDNNTEGVS VDELIENADG FAGVFPEHKY EIVKRLQSRK HICGMTGDGV NDAPALKKAD
610 620 630 640 650 660
IGIAVDDATD AARGASDIVL TEPGLSVIIS AVLTSRAIFQ RMKNYTIYAV SITIRIVMGF
670 680 690 700 710 720
MLLCVFWEFD FPPFMVLVIA ILNDGTIMTI SKDRVKPSPT PDCWKLKEIF ATGVVLGAYL
730 740 750 760 770 780
AIMTVVFFWA AYETNFFHNI FHVRNFNQHH FKMKDKKVAA HLNEQMASAV YLQVSTISQA
790 800 810 820 830 840
LIFVTRSRSW SFVERPGFLL VIAFLIAQLV ASVISAMANW PFAGIRSIGW GWTGVIWIFN
850 860 870 880 890 900
IVTYMLLDPI KFLVRYALSG KSWDRMVEGR TALTGKKNFG QEERMAAWAT EKRTQHGLET
910 920 930 940 950 960
GQKPVYERNS ATELNNMAEE AKRRAEIARM RELQTLKGKV ESAAKLKGYD LEDPNSNNYT
I