Q22099
PR
Gene name |
kars-1 |
Protein name |
Lysine--tRNA ligase |
Names |
Lysyl-tRNA synthetase, LysRS |
Species |
Caenorhabditis elegans |
KEGG Pathway |
cel:CELE_T02G5.9 |
EC number |
6.1.1.6: Ligases forming aminoacyl-tRNA and related compounds |
Protein Class |
|
Descriptions
The autoinhibited protein was predicted that may have potential autoinhibitory elements via cis-regPred.
Autoinhibitory domains (AIDs)
Target domain |
|
Relief mechanism |
|
Assay |
cis-regPred |
Accessory elements
No accessory elements
Autoinhibited structure
Activated structure
1 structures for Q22099
| Entry ID | Method | Resolution | Chain | Position | Source |
|---|---|---|---|---|---|
| AF-Q22099-F1 | Predicted | AlphaFoldDB |
No variants for Q22099
| Variant ID(s) | Position | Change | Description | Diseaes Association | Provenance |
|---|---|---|---|---|---|
| No variants for Q22099 | |||||
No associated diseases with Q22099
5 regional properties for Q22099
| Type | Name | Position | InterPro Accession |
|---|---|---|---|
| domain | Aminoacyl-tRNA synthetase, class II (D/K/N) | 201 - 553 | IPR004364 |
| domain | OB-fold nucleic acid binding domain, AA-tRNA synthetase-type | 105 - 182 | IPR004365 |
| domain | Aminoacyl-tRNA synthetase, class II | 223 - 555 | IPR006195 |
| domain | Lysyl-tRNA synthetase, class II, C-terminal | 216 - 555 | IPR018149 |
| domain | Lysine-tRNA ligase, class II, N-terminal | 104 - 213 | IPR044136 |
Functions
| Description | ||
|---|---|---|
| EC Number | 6.1.1.6 | Ligases forming aminoacyl-tRNA and related compounds |
| Subcellular Localization |
|
|
| PANTHER Family | ||
| PANTHER Subfamily | ||
| PANTHER Protein Class | ||
| PANTHER Pathway Category | No pathway information available | |
3 GO annotations of cellular component
| Name | Definition |
|---|---|
| aminoacyl-tRNA synthetase multienzyme complex | A multienzyme complex found in all multicellular eukaryotes composed of eight proteins with aminoacyl-tRNA synthetase activities (abbreviated as: ArgRS, AspRS, GluProRS, GlnRS, IleRS, LeuRS, LysRS, MetRS where RS is the enzyme, preceded by the amino acid it uses as a substrate) as well as three non-synthetase proteins (p43, p38, and p18) with diverse functions. Several of these subunits are known dimers, so the total polypeptide count in the multisynthetase complex is at least fifteen. All of the enzymes in this assembly catalyze the same reaction, the covalent attachment of an amino acid to either the 2'- or 3'-hydroxyl of the 3'-terminal adenosine of tRNA, but using different substrates. |
| cytosol | The part of the cytoplasm that does not contain organelles but which does contain other particulate matter, such as protein complexes. |
| mitochondrion | A semiautonomous, self replicating organelle that occurs in varying numbers, shapes, and sizes in the cytoplasm of virtually all eukaryotic cells. It is notably the site of tissue respiration. |
4 GO annotations of molecular function
| Name | Definition |
|---|---|
| ATP binding | Binding to ATP, adenosine 5'-triphosphate, a universally important coenzyme and enzyme regulator. |
| lysine-tRNA ligase activity | Catalysis of the reaction: ATP + L-lysine + tRNA(Lys) = AMP + diphosphate + L-lysyl-tRNA(Lys). |
| metal ion binding | Binding to a metal ion. |
| tRNA binding | Binding to a transfer RNA. |
6 GO annotations of biological process
| Name | Definition |
|---|---|
| determination of adult lifespan | The pathways that regulate the duration of the adult phase of the life-cycle of an animal. |
| lysyl-tRNA aminoacylation | The process of coupling lysine to lysyl-tRNA, catalyzed by lysyl-tRNA synthetase. The lysyl-tRNA synthetase is a class-II synthetase. The activated amino acid is transferred to the 3'-OH group of a lysine-accetping tRNA. |
| negative regulation of gene expression | Any process that decreases the frequency, rate or extent of gene expression. Gene expression is the process in which a gene's coding sequence is converted into a mature gene product (protein or RNA). |
| positive regulation of nematode larval development | Any process that increases the rate, frequency, or extent of nematode larval development, the process whose specific outcome is the progression of the nematode larva over time, from its formation to the mature structure. Nematode larval development begins with the newly hatched first-stage larva (L1) and ends with the end of the last larval stage (for example the fourth larval stage (L4) in C. elegans). Each stage of nematode larval development is characterized by proliferation of specific cell lineages and an increase in body size without alteration of the basic body plan. Nematode larval stages are separated by molts in which each stage-specific exoskeleton, or cuticle, is shed and replaced anew. |
| positive regulation of protein phosphorylation | Any process that activates or increases the frequency, rate or extent of addition of phosphate groups to amino acids within a protein. |
| regulation of brood size | Any process that modulates brood size. Brood size is the number of progeny that survive embryogenesis and are cared for at one time. |
7 homologous proteins in AiPD
| UniProt AC | Gene Name | Protein Name | Species | Evidence Code |
|---|---|---|---|---|
| P32048 | MSK1 | Lysine--tRNA ligase, mitochondrial | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) | PR |
| P15180 | KRS1 | Lysine--tRNA ligase, cytoplasmic | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) | PR |
| P0A8N5 | lysU | Lysine--tRNA ligase, heat inducible | Escherichia coli (strain K12) | PR |
| P0A8N3 | lysS | Lysine--tRNA ligase | Escherichia coli (strain K12) | PR |
| Q6F2U9 | Os03g0586800 | Lysine--tRNA ligase | Oryza sativa subsp japonica (Rice) | PR |
| Q9ZPI1 | At3g11710 | Lysine--tRNA ligase, cytoplasmic | Arabidopsis thaliana (Mouse-ear cress) | PR |
| Q43776 | LYSRS | Lysine--tRNA ligase | Solanum lycopersicum (Tomato) (Lycopersicon esculentum) | PR |
| 10 | 20 | 30 | 40 | 50 | 60 |
| MSEPEAKLSK | NEQKRLAKQA | KKEQERLEKD | AAKLNVAVAD | APKVVREADP | SDPQEYFNMR |
| 70 | 80 | 90 | 100 | 110 | 120 |
| VRMIEARRAA | GDNPFPHKFN | VTISLTDFIT | KYTPLEKEQV | VEEIVSVAGR | IHSKRESGSK |
| 130 | 140 | 150 | 160 | 170 | 180 |
| LVFYDIHGEG | THIQIMANAK | FHTGDVDFVT | LHDRIKRGDI | VGFTGRATRT | KAGELSLIPN |
| 190 | 200 | 210 | 220 | 230 | 240 |
| EILQLTPCLH | MLPHSHFGLK | DKELRFRKRY | LDLILNPRVK | DNFVIRSKII | TFLRRYLDNL |
| 250 | 260 | 270 | 280 | 290 | 300 |
| GFLEVETPIM | NQIAGGATAK | PFITHHNDLD | MNLFLRVAPE | LYHKMLVVGG | IDRVYEVGRL |
| 310 | 320 | 330 | 340 | 350 | 360 |
| FRNEGIDLTH | NPEFTTCEFY | MAYADYEDVI | QLTEDLLSSM | VMSIKGTYKI | EYHPNGPNTE |
| 370 | 380 | 390 | 400 | 410 | 420 |
| PVYEVDFTPP | FKRVHMYDGL | AEKLGATLPD | PSTLHTEEAR | EVFDKLCRDN | NVDCSAPRTT |
| 430 | 440 | 450 | 460 | 470 | 480 |
| ARLLDKLVGE | YLESTFISPT | FLIGHPQIMS | PLAKWHRSIP | GLTERFELFA | VTREIANAYT |
| 490 | 500 | 510 | 520 | 530 | 540 |
| ELNDPITQRQ | RFEQQAKDKD | AGDDEAQMID | ETFCNALEYG | LPPTGGWGMG | IDRLSMILTD |
| 550 | 560 | 570 | |||
| NNNIKEVLLF | PAMRPEDGTE | KKTDFDAPTT | LA |