P32048

PR
Gene name
MSK1 (YNL073W, N2364)
Protein name
Lysine--tRNA ligase, mitochondrial
Names
Lysyl-tRNA synthetase, LysRS
Species
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
KEGG Pathway
sce:YNL073W
EC number
6.1.1.6: Ligases forming aminoacyl-tRNA and related compounds
Protein Class

Descriptions

The autoinhibited protein was predicted that may have potential autoinhibitory elements via cis-regPred.

Autoinhibitory domains (AIDs)

1-82 (Predicted disordered autoinhibitory region) PR

Target domain

Relief mechanism

Assay

cis-regPred

Accessory elements

No accessory elements

References

Autoinhibited structure

Activated structure

1 structures for P32048

Entry ID Method Resolution Chain Position Source
AF-P32048-F1 Predicted AlphaFoldDB

16 variants for P32048

Variant ID(s) Position Change Description Diseaes Association Provenance
s14-488588 68 L>F No SGRP
s14-488595 70 Q>R No SGRP
s14-488599 71 E>D No SGRP
s14-488654 90 T>A No SGRP
s14-488760 125 G>A No SGRP
s14-488797 137 L>F No SGRP
s14-488976 197 S>L No SGRP
s14-489386 334 T>A No SGRP
s14-489387 334 T>I No SGRP
s14-489398 338 D>Y No SGRP
s14-489588 401 N>S No SGRP
s14-489632 416 K>E No SGRP
s14-489648 421 S>F No SGRP
s14-489672 429 S>F No SGRP
s14-489827 481 V>I No SGRP
s14-489977 531 V>I No SGRP

No associated diseases with P32048

9 regional properties for P32048

Type Name Position InterPro Accession
domain Aminoacyl-tRNA synthetase, class II (D/K/N) 214 - 568 IPR004364
domain OB-fold nucleic acid binding domain, AA-tRNA synthetase-type 102 - 192 IPR004365
domain Aminoacyl-tRNA synthetase, class II 229 - 566 IPR006195
domain Lysyl-tRNA synthetase, class II, C-terminal 242 - 252 IPR018149-1
domain Lysyl-tRNA synthetase, class II, C-terminal 258 - 274 IPR018149-2
domain Lysyl-tRNA synthetase, class II, C-terminal 286 - 299 IPR018149-3
domain Lysyl-tRNA synthetase, class II, C-terminal 304 - 321 IPR018149-4
domain Lysyl-tRNA synthetase, class II, C-terminal 451 - 467 IPR018149-5
domain Lysine-tRNA ligase, class II, N-terminal 101 - 219 IPR044136

Functions

Description
EC Number 6.1.1.6 Ligases forming aminoacyl-tRNA and related compounds
Subcellular Localization
  • Mitochondrion matrix
PANTHER Family
PANTHER Subfamily
PANTHER Protein Class
PANTHER Pathway Category No pathway information available

2 GO annotations of cellular component

Name Definition
mitochondrial matrix The gel-like material, with considerable fine structure, that lies in the matrix space, or lumen, of a mitochondrion. It contains the enzymes of the tricarboxylic acid cycle and, in some organisms, the enzymes concerned with fatty acid oxidation.
mitochondrion A semiautonomous, self replicating organelle that occurs in varying numbers, shapes, and sizes in the cytoplasm of virtually all eukaryotic cells. It is notably the site of tissue respiration.

3 GO annotations of molecular function

Name Definition
ATP binding Binding to ATP, adenosine 5'-triphosphate, a universally important coenzyme and enzyme regulator.
lysine-tRNA ligase activity Catalysis of the reaction: ATP + L-lysine + tRNA(Lys) = AMP + diphosphate + L-lysyl-tRNA(Lys).
tRNA binding Binding to a transfer RNA.

4 GO annotations of biological process

Name Definition
lysyl-tRNA aminoacylation The process of coupling lysine to lysyl-tRNA, catalyzed by lysyl-tRNA synthetase. The lysyl-tRNA synthetase is a class-II synthetase. The activated amino acid is transferred to the 3'-OH group of a lysine-accetping tRNA.
mitochondrial lysyl-tRNA aminoacylation The process of coupling lysine to lysyl-tRNA in a mitochondrion, catalyzed by lysyl-tRNA synthetase. In tRNA aminoacylation, the amino acid is first activated by linkage to AMP and then transferred to either the 2'- or the 3'-hydroxyl group of the 3'-adenosine residue of the tRNA.
mitochondrial translation The chemical reactions and pathways resulting in the formation of a protein in a mitochondrion. This is a ribosome-mediated process in which the information in messenger RNA (mRNA) is used to specify the sequence of amino acids in the protein; the mitochondrion has its own ribosomes and transfer RNAs, and uses a genetic code that differs from the nuclear code.
tRNA processing The process in which a pre-tRNA molecule is converted to a mature tRNA, ready for addition of an aminoacyl group.

7 homologous proteins in AiPD

UniProt AC Gene Name Protein Name Species Evidence Code
P15180 KRS1 Lysine--tRNA ligase, cytoplasmic Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) PR
P0A8N5 lysU Lysine--tRNA ligase, heat inducible Escherichia coli (strain K12) PR
P0A8N3 lysS Lysine--tRNA ligase Escherichia coli (strain K12) PR
Q6F2U9 Os03g0586800 Lysine--tRNA ligase Oryza sativa subsp japonica (Rice) PR
Q22099 kars-1 Lysine--tRNA ligase Caenorhabditis elegans PR
Q9ZPI1 At3g11710 Lysine--tRNA ligase, cytoplasmic Arabidopsis thaliana (Mouse-ear cress) PR
Q43776 LYSRS Lysine--tRNA ligase Solanum lycopersicum (Tomato) (Lycopersicon esculentum) PR
10 20 30 40 50 60
MNVLLKRRSL TFAPRWLWCK CRSSRSRPYS LAHAVDTSKM EATRRNGQIV KDLGRYYPSM
70 80 90 100 110 120
SESALHDLCQ EYKEVTIADF NERFLGNPAT LHHEDNPNLL LSINGRIKSI RFSGQKIVFI
130 140 150 160 170 180
DLYNGSSGLK NDTQLQLIVN YNKIGGSSED KANFSEYMNF LKKGDYIKAL GYPGFSQSRV
190 200 210 220 230 240
KMLSLICNKL PIVLSVSQLP LPSRLNDETK IKSNRVVDYQ LNGTQTLLVR ARIIKLLRKF
250 260 270 280 290 300
LDDRNFVEVE TPILSSKSNG AMAKPFITSS KDFDHLELRI APELWLKRLI ISGLQKVYEI
310 320 330 340 350 360
GKVFRNEGID STHNAEFSTL EFYETYMSMD DIVTRTEDLF KFLITNLQKF FQDTRLPVPK
370 380 390 400 410 420
TFSELHLALS ENNWKFRKVE FLPTLNKELG IDLMNSGLDI NKPSELLKAL PKDIAKKYFP
430 440 450 460 470 480
SADNTGQLSS LQILNKLSDV FLEQRHCQST LPTVIYHQPA ILSPLAKTDP QNKQVTKRFE
490 500 510 520 530 540
VFIKGKEYIN AYEEENCPQL QLQKFLQQKQ INELTGNKTE TLSPVIDYQY VETMKYGMPP
550 560 570
VGGFGLGIDR LCMLFCDKKR IEEVLPFGCV DDVNRQ