P15180

PR
Gene name
KRS1 (GCD5, YDR037W, YD9673.09)
Protein name
Lysine--tRNA ligase, cytoplasmic
Names
Lysyl-tRNA synthetase, LysRS
Species
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
KEGG Pathway
sce:YDR037W
EC number
6.1.1.6: Ligases forming aminoacyl-tRNA and related compounds
Protein Class

Descriptions

The autoinhibited protein was predicted that may have potential autoinhibitory elements via cis-regPred.

Autoinhibitory domains (AIDs)

1-130 (Predicted disordered autoinhibitory region) PR

Target domain

Relief mechanism

Assay

cis-regPred

Accessory elements

No accessory elements

References

Autoinhibited structure

Activated structure

2 structures for P15180

Entry ID Method Resolution Chain Position Source
7F6W X-ray 261 A A 65-581 PDB
AF-P15180-F1 Predicted AlphaFoldDB

14 variants for P15180

Variant ID(s) Position Change Description Diseaes Association Provenance
s04-525474 13 G>D No SGRP
s04-525501 22 A>V No SGRP
s04-525587 51 A>T No SGRP
s04-525631 65 F>L No SGRP
s04-525729 98 H>R No SGRP
s04-525876 147 G>A No SGRP
s04-525920 162 C>R No SGRP
s04-525921 162 C>Y No SGRP
s04-525931 165 D>E No SGRP
s04-526212 259 R>K No SGRP
s04-526534 366 K>N No SGRP
s04-526724 430 I>V No SGRP
s04-526731 432 V>A No SGRP
s04-527207 591 N>H No SGRP

No associated diseases with P15180

5 regional properties for P15180

Type Name Position InterPro Accession
domain Aminoacyl-tRNA synthetase, class II (D/K/N) 225 - 576 IPR004364
domain OB-fold nucleic acid binding domain, AA-tRNA synthetase-type 124 - 209 IPR004365
domain Aminoacyl-tRNA synthetase, class II 250 - 578 IPR006195
domain Lysyl-tRNA synthetase, class II, C-terminal 240 - 578 IPR018149
domain Lysine-tRNA ligase, class II, N-terminal 123 - 237 IPR044136

Functions

Description
EC Number 6.1.1.6 Ligases forming aminoacyl-tRNA and related compounds
Subcellular Localization
  • Cytoplasm
PANTHER Family
PANTHER Subfamily
PANTHER Protein Class
PANTHER Pathway Category No pathway information available

2 GO annotations of cellular component

Name Definition
cytoplasm The contents of a cell excluding the plasma membrane and nucleus, but including other subcellular structures.
cytosol The part of the cytoplasm that does not contain organelles but which does contain other particulate matter, such as protein complexes.

4 GO annotations of molecular function

Name Definition
ATP binding Binding to ATP, adenosine 5'-triphosphate, a universally important coenzyme and enzyme regulator.
lysine-tRNA ligase activity Catalysis of the reaction: ATP + L-lysine + tRNA(Lys) = AMP + diphosphate + L-lysyl-tRNA(Lys).
mRNA binding Binding to messenger RNA (mRNA), an intermediate molecule between DNA and protein. mRNA includes UTR and coding sequences, but does not contain introns.
tRNA binding Binding to a transfer RNA.

1 GO annotations of biological process

Name Definition
lysyl-tRNA aminoacylation The process of coupling lysine to lysyl-tRNA, catalyzed by lysyl-tRNA synthetase. The lysyl-tRNA synthetase is a class-II synthetase. The activated amino acid is transferred to the 3'-OH group of a lysine-accetping tRNA.

7 homologous proteins in AiPD

UniProt AC Gene Name Protein Name Species Evidence Code
P32048 MSK1 Lysine--tRNA ligase, mitochondrial Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) PR
P0A8N5 lysU Lysine--tRNA ligase, heat inducible Escherichia coli (strain K12) PR
P0A8N3 lysS Lysine--tRNA ligase Escherichia coli (strain K12) PR
Q6F2U9 Os03g0586800 Lysine--tRNA ligase Oryza sativa subsp japonica (Rice) PR
Q22099 kars-1 Lysine--tRNA ligase Caenorhabditis elegans PR
Q9ZPI1 At3g11710 Lysine--tRNA ligase, cytoplasmic Arabidopsis thaliana (Mouse-ear cress) PR
Q43776 LYSRS Lysine--tRNA ligase Solanum lycopersicum (Tomato) (Lycopersicon esculentum) PR
10 20 30 40 50 60
MSQQDNVKAA AEGVANLHLD EATGEMVSKS ELKKRIKQRQ VEAKKAAKKA AAQPKPASKK
70 80 90 100 110 120
KTDLFADLDP SQYFETRSRQ IQELRKTHEP NPYPHKFHVS ISNPEFLAKY AHLKKGETLP
130 140 150 160 170 180
EEKVSIAGRI HAKRESGSKL KFYVLHGDGV EVQLMSQLQD YCDPDSYEKD HDLLKRGDIV
190 200 210 220 230 240
GVEGYVGRTQ PKKGGEGEVS VFVSRVQLLT PCLHMLPADH FGFKDQETRY RKRYLDLIMN
250 260 270 280 290 300
KDARNRFITR SEIIRYIRRF LDQRKFIEVE TPMMNVIAGG ATAKPFITHH NDLDMDMYMR
310 320 330 340 350 360
IAPELFLKQL VVGGLDRVYE IGRQFRNEGI DMTHNPEFTT CEFYQAYADV YDLMDMTELM
370 380 390 400 410 420
FSEMVKEITG SYIIKYHPDP ADPAKELELN FSRPWKRINM IEELEKVFNV KFPSGDQLHT
430 440 450 460 470 480
AETGEFLKKI LVDNKLECPP PLTNARMLDK LVGELEDTCI NPTFIFGHPQ MMSPLAKYSR
490 500 510 520 530 540
DQPGLCERFE VFVATKEICN AYTELNDPFD QRARFEEQAR QKDQGDDEAQ LVDETFCNAL
550 560 570 580 590
EYGLPPTGGW GCGIDRLAMF LTDSNTIREV LLFPTLKPDV LREEVKKEEE N