AiPD: Autoinhibited Protein Database

Gene name	CKX6 (CKX7, At3g63440, MAA21_70)
Protein name	Cytokinin dehydrogenase 6
Names	Cytokinin oxidase 6, AtCKX6, AtCKX7, CKO6
Species	Arabidopsis thaliana (Mouse-ear cress)
KEGG Pathway	ath:AT3G63440
EC number	1.5.99.12: With other acceptors
Protein Class

The autoinhibited protein was predicted that may have potential autoinhibitory elements via cis-regPred.

76-145 (Predicted disordered autoinhibitory region) PR

76-145 (Predicted disordered autoinhibitory region)

cis-regPred

No accessory elements

Entry ID	Method	Resolution	Chain	Position	Source
AF-Q9LY71-F1	Predicted				AlphaFoldDB

Variant ID(s)	Position	Change	Diseaes Association	Provenance
ENSVATH12898443	24	L>F	No	1000Genomes
ENSVATH12898465	96	T>S	No	1000Genomes
tmp_3_23424651_A_G	121	I>V	No	1000Genomes
tmp_3_23424743_G_T	151	E>D	No	1000Genomes
ENSVATH02539694	155	N>S	No	1000Genomes
ENSVATH14473807	158	H>R	No	1000Genomes
ENSVATH06365260	161	L>V	No	1000Genomes
ENSVATH06365264	242	R>S	No	1000Genomes
ENSVATH02539695	250	T>N	No	1000Genomes
ENSVATH12898486	276	Q>H	No	1000Genomes
ENSVATH06365266	276	Q>K	No	1000Genomes
ENSVATH00803232	315	F>S	No	1000Genomes
ENSVATH06365268	333	N>S	No	1000Genomes
ENSVATH00803233	339	Q>K	No	1000Genomes
ENSVATH06365270	363	E>K	No	1000Genomes
tmp_3_23425767_A_C	396	S>R	No	1000Genomes
ENSVATH14473810	410	L>V	No	1000Genomes
ENSVATH02539698	414	S>N	No	1000Genomes
ENSVATH02539699	415	N>K	No	1000Genomes
tmp_3_23425972_C_G	437	P>A	No	1000Genomes
tmp_3_23426027_C_T	455	S>L	No	1000Genomes
ENSVATH12898489	504	G>E	No	1000Genomes

Type	Name	Position	InterPro Accession
binding_site	Oxygen oxidoreductase covalent FAD-binding site	59 - 94	IPR006093
domain	FAD linked oxidase, N-terminal	59 - 205	IPR006094
domain	Cytokinin dehydrogenase 1, FAD/cytokinin binding domain	237 - 516	IPR015345
domain	FAD-binding domain, PCMH-type	55 - 236	IPR016166

		Description
EC Number	1.5.99.12	With other acceptors
Subcellular Localization	Secreted, extracellular space
PANTHER Family
PANTHER Subfamily
PANTHER Protein Class
PANTHER Pathway Category	No pathway information available

Name	Definition
extracellular space	That part of a multicellular organism outside the cells proper, usually taken to be outside the plasma membranes, and occupied by fluid.

Name	Definition
cytokinin dehydrogenase activity	Catalysis of the reaction: N6-dimethylallyladenine + acceptor + H2O = adenine + 3-methylbut-2-enal + reduced electron acceptor.
FAD binding	Binding to the oxidized form, FAD, of flavin-adenine dinucleotide, the coenzyme or the prosthetic group of various flavoprotein oxidoreductase enzymes.
oxidoreductase activity	Catalysis of an oxidation-reduction (redox) reaction, a reversible chemical reaction in which the oxidation state of an atom or atoms within a molecule is altered. One substrate acts as a hydrogen or electron donor and becomes oxidized, while the other acts as hydrogen or electron acceptor and becomes reduced.

Name	Definition
cytokinin metabolic process	The chemical reactions and pathways involving cytokinins, a class of adenine-derived compounds that can function in plants as growth regulators.
stomatal complex morphogenesis	The process in which the anatomical structures of the stomatal complex are generated and organized. The stomatal complex is the stomatal guard cells and their associated epidermal cells.

UniProt AC	Gene Name	Protein Name	Species	Evidence Code
Q6YW51	CKX6	Cytokinin dehydrogenase 6	Oryza sativa subsp japonica (Rice)	PR
Q6YW50	CKX7	Cytokinin dehydrogenase 7	Oryza sativa subsp japonica (Rice)	PR
A3AVP1	CKX8	Cytokinin dehydrogenase 8	Oryza sativa subsp japonica (Rice)	PR
Q8LNV6	CKX3	Cytokinin dehydrogenase 3	Oryza sativa subsp japonica (Rice)	PR
Q5JLP4	CKX4	Cytokinin dehydrogenase 4	Oryza sativa subsp japonica (Rice)	PR
Q94AX4	DLD	D-lactate dehydrogenase [cytochrome], mitochondrial	Arabidopsis thaliana (Mouse-ear cress)	PR
Q9LTS3	CKX3	Cytokinin dehydrogenase 3	Arabidopsis thaliana (Mouse-ear cress)	PR
Q9FUJ2	CKX4	Cytokinin dehydrogenase 4	Arabidopsis thaliana (Mouse-ear cress)	PR