AiPD: Autoinhibited Protein Database

Gene name	RPE65
Protein name	Retinoid isomerohydrolase
Names	All-trans-retinyl-palmitate hydrolase, Lutein isomerase, Meso-zeaxanthin isomerase, Retinal pigment epithelium-specific 65 kDa protein, Retinol isomerase
Species	Bos taurus (Bovine)
KEGG Pathway	bta:282043
EC number	3.1.1.64: Carboxylic ester hydrolases
Protein Class

The autoinhibited protein was predicted that may have potential autoinhibitory elements via cis-regPred.

1-46 (Predicted disordered autoinhibitory region) PR

1-46 (Predicted disordered autoinhibitory region)

cis-regPred

No accessory elements

Entry ID	Method	Resolution	Chain	Position	Source
3FSN	X-ray	214 A	A/B	1-533	PDB
3KVC	X-ray	190 A	A/B	1-533	PDB
4F2Z	X-ray	300 A	A/E	1-533	PDB
4F30	X-ray	315 A	A	1-533	PDB
4F3A	X-ray	260 A	A	1-533	PDB
4F3D	X-ray	250 A	A/B	1-533	PDB
4RSC	X-ray	180 A	A/B	1-533	PDB
4RSE	X-ray	239 A	A/B	2-533	PDB
4RYX	X-ray	200 A	A	1-533	PDB
4RYY	X-ray	230 A	A/B	2-533	PDB
4RYZ	X-ray	250 A	A/B	2-533	PDB
4ZHK	X-ray	209 A	A/B	2-533	PDB
5UL5	X-ray	220 A	A/B	1-533	PDB
5ULG	X-ray	210 A	A/B	1-533	PDB
7K88	X-ray	210 A	A/B	2-533	PDB
7K89	X-ray	215 A	A/B	2-533	PDB
7K8G	X-ray	195 A	A/B	2-533	PDB
7L0E	X-ray	190 A	A/B	1-533	PDB
8DOC	X-ray	210 A	A	2-533	PDB
AF-Q28175-F1	Predicted				AlphaFoldDB

Variant ID(s)	Position	Change	Description	Diseaes Association	Provenance
No variants for Q28175

Type	Name	Position	InterPro Accession
domain	Integrase, catalytic core	136 - 312	IPR001584
domain	HTH domain, IS21 transposase-type	13 - 78	IPR017894

		Description
EC Number	3.1.1.64	Carboxylic ester hydrolases
Subcellular Localization	Cytoplasm Cell membrane ; Lipid-anchor Microsome membrane	Undergoes light-dependent intracellular transport to become more concentrated in the central region of the retina pigment epithelium cells (By similarity) Attached to the membrane by a lipid anchor when palmitoylated (membrane form), soluble when unpalmitoylated
PANTHER Family
PANTHER Subfamily
PANTHER Protein Class
PANTHER Pathway Category	No pathway information available

Name	Definition
endoplasmic reticulum membrane	The lipid bilayer surrounding the endoplasmic reticulum.
membrane	A lipid bilayer along with all the proteins and protein complexes embedded in it an attached to it.
plasma membrane	The membrane surrounding a cell that separates the cell from its external environment. It consists of a phospholipid bilayer and associated proteins.

Name	Definition
all-trans-retinyl-ester hydrolase, 11-cis retinol forming activity	Catalysis of the reaction: H(2)O + all-trans-retinyl ester = 11-cis-retinol + fatty acid.
all-trans-retinyl-palmitate hydrolase, 11-cis retinol forming activity	Catalysis of the reaction: H(2)O + all-trans-retinyl palmitate = 11-cis-retinol + H(+) + palmitate.
cardiolipin binding	Binding to cardiolipin.
identical protein binding	Binding to an identical protein or proteins.
metal ion binding	Binding to a metal ion.
oxidoreductase activity, acting on single donors with incorporation of molecular oxygen, incorporation of two atoms of oxygen	Catalysis of an oxidation-reduction (redox) reaction in which hydrogen or electrons are transferred from one donor, and two oxygen atoms is incorporated into a donor.
phosphatidylcholine binding	Binding to a phosphatidylcholine, a glycophospholipid in which a phosphatidyl group is esterified to the hydroxyl group of choline.
phosphatidylserine binding	Binding to phosphatidylserine, a class of glycophospholipids in which a phosphatidyl group is esterified to the hydroxyl group of L-serine.
retinal isomerase activity	Catalysis of the reaction: all-trans-retinal = 11-cis-retinal.

Name	Definition
response to stimulus	Any process that results in a change in state or activity of a cell or an organism (in terms of movement, secretion, enzyme production, gene expression, etc.) as a result of a stimulus. The process begins with detection of the stimulus and ends with a change in state or activity or the cell or organism.
visual perception	The series of events required for an organism to receive a visual stimulus, convert it to a molecular signal, and recognize and characterize the signal. Visual stimuli are detected in the form of photons and are processed to form an image.
zeaxanthin biosynthetic process	The chemical reactions and pathways resulting in the formation of zeaxanthin.

UniProt AC	Gene Name	Protein Name	Species	Evidence Code
Q9YGX2	RPE65	Retinoid isomerohydrolase	Gallus gallus (Chicken)	PR
Q9TVB8	RPE65	Retinoid isomerohydrolase	Canis lupus familiaris (Dog) (Canis familiaris)	PR
Q16518	RPE65	Retinoid isomerohydrolase	Homo sapiens (Human)	PR
O24592	VP14	9-cis-epoxycarotenoid dioxygenase 1, chloroplastic	Zea mays (Maize)	PR
Q91ZQ5	Rpe65	Retinoid isomerohydrolase	Mus musculus (Mouse)	PR
O70276	Rpe65	Retinoid isomerohydrolase	Rattus norvegicus (Rat)	PR
Q8LIY8	CCD8B	Carotenoid cleavage dioxygenase 8 homolog B, chloroplastic	Oryza sativa subsp japonica (Rice)	PR
Q9C6Z1	NCED5	Probable 9-cis-epoxycarotenoid dioxygenase NCED5, chloroplastic	Arabidopsis thaliana (Mouse-ear cress)	PR
Q9LRR7	NCED3	9-cis-epoxycarotenoid dioxygenase NCED3, chloroplastic	Arabidopsis thaliana (Mouse-ear cress)	PR
Q9LRM7	NCED6	9-cis-epoxycarotenoid dioxygenase NCED6, chloroplastic	Arabidopsis thaliana (Mouse-ear cress)	PR