Q23670

PR
Gene name
top-2
Protein name
DNA topoisomerase 2 top-2
Names
DNA topoisomerase II
Species
Caenorhabditis elegans
KEGG Pathway
cel:CELE_K12D12.1
EC number
5.6.2.2: Enzymes altering nucleic acid conformation
Protein Class

Descriptions

The autoinhibited protein was predicted that may have potential autoinhibitory elements via cis-regPred.

Autoinhibitory domains (AIDs)

1-83 (Predicted disordered autoinhibitory region) PR

Target domain

Relief mechanism

Assay

cis-regPred

Accessory elements

No accessory elements

References

Autoinhibited structure

Activated structure

1 structures for Q23670

Entry ID Method Resolution Chain Position Source
AF-Q23670-F1 Predicted AlphaFoldDB

1 variants for Q23670

Variant ID(s) Position Change Description Diseaes Association Provenance
797 Q>M strain: CB4856; increased sensitivity to etoposide, teniposide and slightly increased sensitivity to XK469 and increased resistance to dactinomycin [UniProt] No

No associated diseases with Q23670

7 regional properties for Q23670

Type Name Position InterPro Accession
domain DNA topoisomerase, type IIA, domain A 728 - 1222 IPR002205
domain Histidine kinase/HSP90-like ATPase 114 - 259 IPR003594
domain TOPRIM domain 490 - 607 IPR006171
domain DNA topoisomerase, type IIA, subunit B, domain 2 301 - 462 IPR013506
conserved_site DNA topoisomerase, type IIA, conserved site 494 - 502 IPR018522
domain C-terminal associated domain of TOPRIM 608 - 746 IPR031660
domain DNA topoisomerase 2, TOPRIM domain 490 - 610 IPR034157

Functions

Description
EC Number 5.6.2.2 Enzymes altering nucleic acid conformation
Subcellular Localization
  • Nucleus, nucleoplasm
  • Chromosome
  • Cytoplasm, cytoskeleton, spindle
  • In the hermaphrodite and male germline, localizes to the nucleoplasm in the proliferative mitotic zone except in metaphase where it localizes to the spindle
  • In the transition zone, associates with chromosomes and localizes along the length of chromosomes in pachytene nuclei
  • In diplotene, starts to relocalize to the nucleoplasm
  • In the oocytes, it is completely disassociated from chromosomes in diakinetic nuclei
  • In the male germ line, it is mostly nucleoplasmic in karyosome nuclei
  • In male meiotic metaphase, surrounds chromosomes and a small pool is detected in the cytoplasm
  • In meiotic anaphase, encircles the chromosomes and localizes between the segregating chromosomes
  • May co-localize with nmad-1 on DNA (Probable)
PANTHER Family
PANTHER Subfamily
PANTHER Protein Class
PANTHER Pathway Category No pathway information available

5 GO annotations of cellular component

Name Definition
chromosome A structure composed of a very long molecule of DNA and associated proteins (e.g. histones) that carries hereditary information.
cytoplasm The contents of a cell excluding the plasma membrane and nucleus, but including other subcellular structures.
nucleoplasm That part of the nuclear content other than the chromosomes or the nucleolus.
nucleus A membrane-bounded organelle of eukaryotic cells in which chromosomes are housed and replicated. In most cells, the nucleus contains all of the cell's chromosomes except the organellar chromosomes, and is the site of RNA synthesis and processing. In some species, or in specialized cell types, RNA metabolism or DNA replication may be absent.
spindle The array of microtubules and associated molecules that forms between opposite poles of a eukaryotic cell during mitosis or meiosis and serves to move the duplicated chromosomes apart.

4 GO annotations of molecular function

Name Definition
ATP binding Binding to ATP, adenosine 5'-triphosphate, a universally important coenzyme and enzyme regulator.
DNA binding Any molecular function by which a gene product interacts selectively and non-covalently with DNA (deoxyribonucleic acid).
DNA topoisomerase type II (double strand cut, ATP-hydrolyzing) activity Catalysis of a DNA topological transformation by transiently cleaving a pair of complementary DNA strands to form a gate through which a second double-stranded DNA segment is passed, after which the severed strands in the first DNA segment are rejoined, driven by ATP hydrolysis. The enzyme changes the linking number in multiples of 2.
metal ion binding Binding to a metal ion.

6 GO annotations of biological process

Name Definition
cell division The process resulting in division and partitioning of components of a cell to form more cells; may or may not be accompanied by the physical separation of a cell into distinct, individually membrane-bounded daughter cells.
DNA topological change The process in which a transformation is induced in the topological structure of a double-stranded DNA helix, resulting in a change in linking number.
mitotic sister chromatid segregation The cell cycle process in which replicated homologous chromosomes are organized and then physically separated and apportioned to two sets during the mitotic cell cycle. Each replicated chromosome, composed of two sister chromatids, aligns at the cell equator, paired with its homologous partner. One homolog of each morphologic type goes into each of the resulting chromosome sets.
mitotic sister chromatid separation The process in which sister chromatids are physically detached from each other during mitosis.
resolution of meiotic recombination intermediates The cleavage and rejoining of intermediates, such as Holliday junctions, formed during meiotic recombination to produce two intact molecules in which genetic material has been exchanged.
sister chromatid segregation The cell cycle process in which sister chromatids are organized and then physically separated and apportioned to two or more sets.

6 homologous proteins in AiPD

UniProt AC Gene Name Protein Name Species Evidence Code
Q02880 TOP2B DNA topoisomerase 2-beta Homo sapiens (Human) PR
P11388 TOP2A DNA topoisomerase 2-alpha Homo sapiens (Human) PR
Q01320 Top2a DNA topoisomerase 2-alpha Mus musculus (Mouse) PR
Q64511 Top2b DNA topoisomerase 2-beta Mus musculus (Mouse) PR
O46374 TOP2A DNA topoisomerase 2-alpha Sus scrofa (Pig) PR
P41516 Top2a DNA topoisomerase 2-alpha Rattus norvegicus (Rat) PR
10 20 30 40 50 60
MSDSDSEFSI EDSPKKKTAP KKEKASPKKK KDDANESMVM TEEDRNVFTS IDKKGGGSKQ
70 80 90 100 110 120
MAIEDIYQKK SQLEHILLRP DTYIGSVEHT EKTPMWVYNM EESKLEQRDI SYVPGLYKIY
130 140 150 160 170 180
DEILVNAADN KQRDPKMNTI KITINKEKNE ISVYNNGKGI PVTQHKVEKV YVPELIFGTL
190 200 210 220 230 240
LTSSNYNDDE KKVTGGRNGY GAKLCNIFST KFTLETSSRD YKSAFKQTWI KNMTRDEEPK
250 260 270 280 290 300
IVKSTDEDFT KITFSPDLAK FKMKELDDDI CHLMARRAYD VAGSSKGVAV FLNGKRIPIK
310 320 330 340 350 360
GFEDYVQMYT SQFNNEGEPL KIAYEQVGDR WQVALALSEK GFQQVSFVNS IATTKGGRHV
370 380 390 400 410 420
DYVADQMVAK FIDSIKRKLT KTSMNIKPFQ IKNHMWVFVN CLIENPTFDS QTKETMTLQQ
430 440 450 460 470 480
KQFGSTCVLS EKFSKAASSV GITDAVMSWV RFKQMDDLNK KCSKTKTSKL KGIPKLEDAN
490 500 510 520 530 540
DAGTKNSQQC TLILTEGDSA KTLAVSGLSV VGRDKYGVFP LRGKLLNVRE GNMKQIADNA
550 560 570 580 590 600
EVNAMIKILG LQYKKKYETE DDFKTLRYGK LMVMADQDQD GSHIKGLVIN FIHHFWPSLI
610 620 630 640 650 660
QRNFVEEFIT PIVKATKGKE EVSFYSLPEY SEWRMNTDNW KSYKIKYYKG LGTSTSKEAK
670 680 690 700 710 720
EYFLDMVRHR IRFKYNGADD DNAVDMAFSK KKIEERKDWL SKWMREKKDR KQQGLAEEYL
730 740 750 760 770 780
YNKDTRFVTF KDFVNRELVL FSNLDNERSI PCLVDGFKPG QRKVLFACFK RADKREVKVA
790 800 810 820 830 840
QLAGAVAEIS AYHHGEQSLM GTIVNLAQDY VGSNNINLLL PIGQFGTRLQ GGKDSASARY
850 860 870 880 890 900
IFTQLSPVTR TLFPAHDDNV LRFLYEENQR IEPEWYCPII PMVLVNGAQG IGTGWSTNIP
910 920 930 940 950 960
NYNPRELVKN IKRLIAGEPQ KALAPWYKNF RGKIIQIDPS RFACYGEVAV LDDNTIEITE
970 980 990 1000 1010 1020
LPIKQWTQDY KEKVLEGLME SSDKKSPVIV DYKEYHTDTT VKFVVKLSPG KLRELERGQD
1030 1040 1050 1060 1070 1080
LHQVFKLQAV INTTCMVLFD AAGCLRTYTS PEAITQEFYD SRQEKYVQRK EYLLGVLQAQ
1090 1100 1110 1120 1130 1140
SKRLTNQARF ILAKINNEIV LENKKKAAIV DVLIKMKFDA DPVKKWKEEQ KLKELRESGE
1150 1160 1170 1180 1190 1200
IELDEDDLAA VAVEEDEAIS SAAKAVETKL SDYDYLVGMA LIKLSEEEKN KLIKESEEKM
1210 1220 1230 1240 1250 1260
AEVRVIEKKT WQDLWHEDLD NFVSELDKQE AREKADQDAS IKNAAKKLAA DAKTGRGPKK
1270 1280 1290 1300 1310 1320
NVCTEVLPSK DGQRIEPMLD AATKAKYEKM SQPKKERVKK EPKEPKEPKK VKKEGQDIKK
1330 1340 1350 1360 1370 1380
FMSPAAPKTA KKEKSDGFNS DMSEESDVEF DEGIDFDSDD DGVEREDVVS KPKPRTGKGA
1390 1400 1410 1420 1430 1440
AKAEVIDLSD DDEVPAKKPA PAKKAAPKKK KSEFSDLSGG DSDEEAEKKP STSKKPSPKK
1450 1460 1470 1480 1490 1500
AAPKTAEPKS KAVTDFFGAS KKNGKKAAGS DDEDDESFVV APREKSGRAR KAPPTYDVDS
1510
GSDSDQPKKK RGRVVDSDSD