O46374

PR
Gene name
TOP2A
Protein name
DNA topoisomerase 2-alpha
Names
DNA topoisomerase II, alpha isozyme
Species
Sus scrofa (Pig)
KEGG Pathway
ssc:396917
EC number
5.6.2.2: Enzymes altering nucleic acid conformation
Protein Class

Descriptions

The autoinhibited protein was predicted that may have potential autoinhibitory elements via cis-regPred.

Autoinhibitory domains (AIDs)

1171-1533 (Predicted disordered autoinhibitory region) PR

Target domain

Relief mechanism

Assay

cis-regPred

Accessory elements

No accessory elements

References

Autoinhibited structure

Activated structure

1 structures for O46374

Entry ID Method Resolution Chain Position Source
AF-O46374-F1 Predicted AlphaFoldDB

19 variants for O46374

Variant ID(s) Position Change Description Diseaes Association Provenance
rs694256731 104 C>Y No EVA
rs326109374 193 T>M No EVA
rs711660400 205 E>* No EVA
rs699060378 268 R>H No EVA
rs708828004 296 R>S No EVA
rs711788852 330 A>D No EVA
rs690513634 608 W>C No EVA
rs701635576 624 A>S No EVA
rs704977584 809 M>L No EVA
rs338639498 883 E>K No EVA
rs704645909 889 L>I No EVA
rs698619764 942 P>T No EVA
rs1112716107 1076 V>L No EVA
rs713683842 1274 T>A No EVA
rs320743075 1383 A>T No EVA
rs333654134 1440 P>L No EVA
rs81218293 1449 L>W No EVA
rs707807484 1489 T>I No EVA
rs700520882 1514 S>Y No EVA

No associated diseases with O46374

12 regional properties for O46374

Type Name Position InterPro Accession
domain RNA recognition motif domain 2 - 75 IPR000504-1
domain RNA recognition motif domain 81 - 156 IPR000504-2
domain K Homology domain 194 - 265 IPR004087-1
domain K Homology domain 275 - 348 IPR004087-2
domain K Homology domain 404 - 475 IPR004087-3
domain K Homology domain 486 - 558 IPR004087-4
domain K Homology domain, type 1 198 - 262 IPR004088-1
domain K Homology domain, type 1 280 - 344 IPR004088-2
domain K Homology domain, type 1 409 - 471 IPR004088-3
domain K Homology domain, type 1 490 - 554 IPR004088-4
domain IGF2BP1, RNA recognition motif 1 1 - 77 IPR034837
domain IGF2BP1, RNA recognition motif 2 81 - 156 IPR034842

Functions

Description
EC Number 5.6.2.2 Enzymes altering nucleic acid conformation
Subcellular Localization
  • Cytoplasm
  • Nucleus, nucleoplasm
  • Nucleus
  • Nucleus, nucleolus
PANTHER Family
PANTHER Subfamily
PANTHER Protein Class
PANTHER Pathway Category No pathway information available

11 GO annotations of cellular component

Name Definition
centriole A cellular organelle, found close to the nucleus in many eukaryotic cells, consisting of a small cylinder with microtubular walls, 300-500 nm long and 150-250 nm in diameter. It contains nine short, parallel, peripheral microtubular fibrils, each fibril consisting of one complete microtubule fused to two incomplete microtubules. Cells usually have two centrioles, lying at right angles to each other. At division, each pair of centrioles generates another pair and the twin pairs form the pole of the mitotic spindle.
chromosome, centromeric region The region of a chromosome that includes the centromeric DNA and associated proteins. In monocentric chromosomes, this region corresponds to a single area of the chromosome, whereas in holocentric chromosomes, it is evenly distributed along the chromosome.
condensed chromosome A highly compacted molecule of DNA and associated proteins resulting in a cytologically distinct structure.
cytoplasm The contents of a cell excluding the plasma membrane and nucleus, but including other subcellular structures.
DNA topoisomerase type II (double strand cut, ATP-hydrolyzing) complex Complex that possesses DNA topoisomerase II (double strand cut, ATP-hydrolyzing) activity.
male germ cell nucleus The nucleus of a male germ cell, a reproductive cell in males.
nuclear chromosome A chromosome that encodes the nuclear genome and is found in the nucleus of a eukaryotic cell during the cell cycle phases when the nucleus is intact.
nucleolus A small, dense body one or more of which are present in the nucleus of eukaryotic cells. It is rich in RNA and protein, is not bounded by a limiting membrane, and is not seen during mitosis. Its prime function is the transcription of the nucleolar DNA into 45S ribosomal-precursor RNA, the processing of this RNA into 5.8S, 18S, and 28S components of ribosomal RNA, and the association of these components with 5S RNA and proteins synthesized outside the nucleolus. This association results in the formation of ribonucleoprotein precursors; these pass into the cytoplasm and mature into the 40S and 60S subunits of the ribosome.
nucleoplasm That part of the nuclear content other than the chromosomes or the nucleolus.
nucleus A membrane-bounded organelle of eukaryotic cells in which chromosomes are housed and replicated. In most cells, the nucleus contains all of the cell's chromosomes except the organellar chromosomes, and is the site of RNA synthesis and processing. In some species, or in specialized cell types, RNA metabolism or DNA replication may be absent.
ribonucleoprotein complex A macromolecular complex that contains both RNA and protein molecules.

11 GO annotations of molecular function

Name Definition
ATP binding Binding to ATP, adenosine 5'-triphosphate, a universally important coenzyme and enzyme regulator.
chromatin binding Binding to chromatin, the network of fibers of DNA, protein, and sometimes RNA, that make up the chromosomes of the eukaryotic nucleus during interphase.
DNA binding, bending The activity of binding selectively and non-covalently to and distorting the original structure of DNA, typically a straight helix, into a bend, or increasing the bend if the original structure was intrinsically bent due to its sequence.
DNA topoisomerase type II (double strand cut, ATP-hydrolyzing) activity Catalysis of a DNA topological transformation by transiently cleaving a pair of complementary DNA strands to form a gate through which a second double-stranded DNA segment is passed, after which the severed strands in the first DNA segment are rejoined, driven by ATP hydrolysis. The enzyme changes the linking number in multiples of 2.
histone deacetylase binding Binding to histone deacetylase.
magnesium ion binding Binding to a magnesium (Mg) ion.
protein C-terminus binding Binding to a protein C-terminus, the end of a peptide chain at which the 1-carboxyl function of a constituent amino acid is not attached in peptide linkage to another amino-acid residue.
protein heterodimerization activity Binding to a nonidentical protein to form a heterodimer.
protein homodimerization activity Binding to an identical protein to form a homodimer.
protein kinase C binding Binding to protein kinase C.
ubiquitin binding Binding to ubiquitin, a protein that when covalently bound to other cellular proteins marks them for proteolytic degradation.

15 GO annotations of biological process

Name Definition
apoptotic chromosome condensation The compaction of chromatin during apoptosis.
cellular response to DNA damage stimulus Any process that results in a change in state or activity of a cell (in terms of movement, secretion, enzyme production, gene expression, etc.) as a result of a stimulus indicating damage to its DNA from environmental insults or errors during metabolism.
DNA ligation The re-formation of a broken phosphodiester bond in the DNA backbone, carried out by DNA ligase.
DNA topological change The process in which a transformation is induced in the topological structure of a double-stranded DNA helix, resulting in a change in linking number.
embryonic cleavage The first few specialized divisions of an activated animal egg.
female meiotic nuclear division A cell cycle process by which the cell nucleus divides as part of a meiotic cell cycle in the female germline.
hematopoietic progenitor cell differentiation The process in which precursor cell type acquires the specialized features of a hematopoietic progenitor cell, a class of cell types including myeloid progenitor cells and lymphoid progenitor cells.
negative regulation of DNA duplex unwinding Any process that stops, prevents or reduces the frequency, rate or extent of DNA duplex unwinding.
positive regulation of apoptotic process Any process that activates or increases the frequency, rate or extent of cell death by apoptotic process.
positive regulation of single stranded viral RNA replication via double stranded DNA intermediate Any process that activates or increases the frequency, rate or extent of retroviral genome replication.
positive regulation of transcription by RNA polymerase II Any process that activates or increases the frequency, rate or extent of transcription from an RNA polymerase II promoter.
regulation of circadian rhythm Any process that modulates the frequency, rate or extent of a circadian rhythm. A circadian rhythm is a biological process in an organism that recurs with a regularity of approximately 24 hours.
resolution of meiotic recombination intermediates The cleavage and rejoining of intermediates, such as Holliday junctions, formed during meiotic recombination to produce two intact molecules in which genetic material has been exchanged.
rhythmic process Any process pertinent to the generation and maintenance of rhythms in the physiology of an organism.
sister chromatid segregation The cell cycle process in which sister chromatids are organized and then physically separated and apportioned to two or more sets.

6 homologous proteins in AiPD

UniProt AC Gene Name Protein Name Species Evidence Code
Q02880 TOP2B DNA topoisomerase 2-beta Homo sapiens (Human) PR
P11388 TOP2A DNA topoisomerase 2-alpha Homo sapiens (Human) PR
Q64511 Top2b DNA topoisomerase 2-beta Mus musculus (Mouse) PR
Q01320 Top2a DNA topoisomerase 2-alpha Mus musculus (Mouse) PR
P41516 Top2a DNA topoisomerase 2-alpha Rattus norvegicus (Rat) PR
Q23670 top-2 DNA topoisomerase 2 top-2 Caenorhabditis elegans PR
10 20 30 40 50 60
MEVSPLQPVN ENMQVNKTKK NEEAKKRLSI ERIYQKKTQL EHILLRPDTY IGSVESVTQQ
70 80 90 100 110 120
MWVYDEDIGI NYREVTFVPG LYKIFDEILV NAADNKQRDP KMSCIRVTID PENNLISIWN
130 140 150 160 170 180
NGKGIPVVEH KVEKMYVPAL IFGQLLTSSN YDDEEKKVTG GRNGYGAKLC NIFSTKFTVE
190 200 210 220 230 240
TASREYKKMF KQTWMDNMGR AGEMELKPFN GEDYTCITFH PDLSKFKMQS LDKDIVALMV
250 260 270 280 290 300
RRAYDIAGST KDVKVFLNGN KLPVKGFRSY VDLYLKDKVD ETGNPLKIIH EQVNHRWEVC
310 320 330 340 350 360
LTMSEKGFQQ ISFVNSIATS KGGRHVDYVA DQIVAKLVDV VKKKNKGGVA VKAHQVKNHM
370 380 390 400 410 420
WIFVNALIEN PTFDSQTKEN MTLQVKSFGS TCQLSEKFIK AAIGCGIVES ILNWVKFKAQ
430 440 450 460 470 480
VQLNKKCSAV KHNRIKGIPK LDDANDAGGR NSTECTLILT EGDSAKTLAV SGLGVVGRDK
490 500 510 520 530 540
YGVFPLRGKI LNVREASHKQ IMENAEINNI IKIVGLQYKK NYEDEDSLKT LRYGKIMIMT
550 560 570 580 590 600
DQDQDGSHIK GLLINFIHHN WPSLLRHRFL EEFITPIVKV SKNKQEMAFY SLPEFEEWKS
610 620 630 640 650 660
STPNHKKWKV KYYKGLGTST SKEAKEYFAD MKRHRIQFKY SGPEDDAAIS LAFSKKQIDD
670 680 690 700 710 720
RKEWLTHFME DRRQRKLLGL PEDYLYGQTT TYLTYNDFIN KELILFSNSD NERSIPSMVD
730 740 750 760 770 780
GLKPGQRKVL FTCFKRNDKR EVKVAQLAGS VAEMSSYHHG EMSLMMTIIN LAQNFVGSNN
790 800 810 820 830 840
LNLLQPIGQF GTRLHGGKDS ASPRYIFTML SPLARLLFPP KDDHTLKFLY DDNQRVEPEW
850 860 870 880 890 900
YIPIIPMVLI NGAEGIGTGW SCKIPNFDVR EVVNNIRLLM DGEEPLPMLP SYKNFKGTIE
910 920 930 940 950 960
ELAPNQYVIS GEVAILNSTT IEISELPIRT WTQTYKEQVL EPMLNGTEKT PPLITDYREY
970 980 990 1000 1010 1020
HTDTTVKFVV KMTEEKLAEA ERVGLHKVFK LQTSLTCNSM VLFDHVGCLK KYDTVLDILR
1030 1040 1050 1060 1070 1080
DFFELRLKYY GLRKEWLLGM LGAESAKLNN QARFILEKID GKIIIENKPK KELIKVLIQR
1090 1100 1110 1120 1130 1140
GYDSDPVKAW KEAQQKVPDE EENEESDNEK EADKSDSVAD SGPTFNYLLD MPLWYLTKEK
1150 1160 1170 1180 1190 1200
KDELCKLRNE KEQELETLKR KSPSDLWKED LAAFIEELEA VEAKEKQDEQ IGLPGKGGKA
1210 1220 1230 1240 1250 1260
KGKKTQMAEV LPSPCGKRVI PRVTVEMKAE AEKKIKKKIK SENTEGSPQE DGMEVEGLKQ
1270 1280 1290 1300 1310 1320
RLEKKQKREP GTKTKKQTTL PFKPIKKAKK RNPWSDSESD ISSDESNFNV PPREKEPRRA
1330 1340 1350 1360 1370 1380
AAKTKFTVDL DSDEDFSDAD EKTRDEDFVP SDTSPQKAET SPKHTNKEPK PQKSTPSVSD
1390 1400 1410 1420 1430 1440
FDADDAKDNV PPSPSSPVAD FPAVTETIKP VSKKNVTVKK TAAKSQSSTS TTGAKKRAAP
1450 1460 1470 1480 1490 1500
KGAKKDPDLD SDVSKKPNPP KPKGRRKRKP STSDDSDSNF EKMISKAVTS KKPKGESDDF
1510 1520 1530
HLDLDLAVAS RAKSGRTKKP IKYLEESDED DLF