Q01320

PR
Gene name
Top2a (Top-2, Top2)
Protein name
DNA topoisomerase 2-alpha
Names
DNA topoisomerase II, alpha isozyme
Species
Mus musculus (Mouse)
KEGG Pathway
mmu:21973
EC number
5.6.2.2: Enzymes altering nucleic acid conformation
Protein Class

Descriptions

The autoinhibited protein was predicted that may have potential autoinhibitory elements via cis-regPred.

Autoinhibitory domains (AIDs)

1-60 (Predicted disordered autoinhibitory region) PR

Target domain

Relief mechanism

Assay

cis-regPred

Accessory elements

No accessory elements

References

Autoinhibited structure

Activated structure

1 structures for Q01320

Entry ID Method Resolution Chain Position Source
AF-Q01320-F1 Predicted AlphaFoldDB

87 variants for Q01320

Variant ID(s) Position Change Description Diseaes Association Provenance
rs3389210486 47 D>E No EVA
rs3389210453 77 V>D No EVA
rs3412986317 83 I>N No EVA
rs3389169566 128 E>K No EVA
rs3389208978 141 F>L No EVA
rs3389200014 255 F>S No EVA
rs3389136406 258 G>R No EVA
rs214544679 260 S>R No EVA
rs3389200401 268 S>E* No EVA
rs3389210414 269 Y>* No EVA
rs3389169548 269 Y>D No EVA
rs3389203170 271 D>E No EVA
rs3389200018 272 L>V No EVA
rs3413085460 273 Y>* No EVA
rs3389203123 289 H>Y No EVA
rs3389161682 294 P>S No EVA
rs3402687743 313 V>I No EVA
rs3389136481 340 V>M No EVA
rs3389174988 341 K>N No EVA
rs3402985264 349 A>T No EVA
rs3389200459 357 N>Y No EVA
rs3389203127 362 F>C No EVA
rs3389203090 380 M>L No EVA
rs3389193455 393 L>V No EVA
rs3389202978 396 K>R No EVA
rs3389202977 407 V>M No EVA
rs3389214877 491 N>D No EVA
rs3389195385 554 N>I No EVA
rs3389175015 577 V>L No EVA
rs3389202888 578 K>N No EVA
rs3389210450 659 D>H No EVA
rs3402286533 662 E>A No EVA
rs3389200445 703 I>T No EVA
rs3402677622 723 P>Q No EVA
rs3389199096 789 F>L No EVA
rs3389214920 805 I>N No EVA
rs3389202952 822 D>V No EVA
rs3389195390 842 P>S No EVA
rs3389199055 868 V>L No EVA
rs3389195382 907 V>M No EVA
rs3389169514 911 E>G No EVA
rs3389210409 917 S>Y No EVA
rs3389173651 946 T>I No EVA
rs3389173680 968 V>L No EVA
rs3389193509 983 G>D No EVA
rs3389193484 986 K>E No EVA
rs13461510 1022 F>L No EVA
rs3389169568 1034 E>K No EVA
rs13461511 1057 K>E No EVA
rs13461505 1116 A>V No EVA
rs3402686673 1124 Y>H No EVA
rs3402749986 1128 M>I No EVA
rs3389169521 1143 K>R No EVA
rs13461512 1144 Q>R No EVA
rs3389169576 1159 S>I No EVA
rs3389193479 1169 A>T No EVA
rs3389169557 1171 F>L No EVA
rs3389161752 1177 V>SDG* No EVA
rs3389203140 1180 A>G No EVA
rs3389203162 1182 E>G No EVA
rs237524109 1201 K>R No EVA
rs3389193480 1203 Q>E No EVA
rs3389200394 1254 G>E No EVA
rs251282901 1257 R>Q No EVA
rs13461508 1266 K>R No EVA
rs13461506 1281 V>I No EVA
rs252070217 1285 R>K No EVA
rs3402286485 1290 W>R No EVA
rs3402686624 1290 W>S No EVA
rs227826014 1297 V>M No EVA
rs27025981 1310 Q>E No EVA
rs27025981 1310 Q>K No EVA
rs234509752 1315 S>R No EVA
rs27025985 1327 D>A No EVA
rs250006781 1327 D>N No EVA
rs260878449 1331 D>E No EVA
rs247683655 1368 Q>K No EVA
rs3389208965 1404 K>T No EVA
rs27025989 1409 T>A No EVA
rs27025992 1425 T>A No EVA
rs258006384 1453 P>L No EVA
rs3389199111 1463 K>E No EVA
rs27025994 1466 S>C No EVA
rs239955303 1488 K>R No EVA
rs219798048 1491 E>K No EVA
rs27025996 1493 D>N No EVA
rs234344892 1509 D>E No EVA

No associated diseases with Q01320

8 regional properties for Q01320

Type Name Position InterPro Accession
domain DNA topoisomerase, type IIA, domain A 692 - 1171 IPR002205
domain Histidine kinase/HSP90-like ATPase 78 - 223 IPR003594
domain TOPRIM domain 454 - 571 IPR006171
domain DTHCT 1431 - 1518 IPR012542
domain DNA topoisomerase, type IIA, subunit B, domain 2 265 - 425 IPR013506
conserved_site DNA topoisomerase, type IIA, conserved site 458 - 466 IPR018522
domain C-terminal associated domain of TOPRIM 572 - 710 IPR031660
domain DNA topoisomerase 2, TOPRIM domain 454 - 574 IPR034157

Functions

Description
EC Number 5.6.2.2 Enzymes altering nucleic acid conformation
Subcellular Localization
  • Cytoplasm
  • Nucleus, nucleoplasm
  • Nucleus
  • Nucleus, nucleolus
PANTHER Family
PANTHER Subfamily
PANTHER Protein Class
PANTHER Pathway Category No pathway information available

12 GO annotations of cellular component

Name Definition
centriole A cellular organelle, found close to the nucleus in many eukaryotic cells, consisting of a small cylinder with microtubular walls, 300-500 nm long and 150-250 nm in diameter. It contains nine short, parallel, peripheral microtubular fibrils, each fibril consisting of one complete microtubule fused to two incomplete microtubules. Cells usually have two centrioles, lying at right angles to each other. At division, each pair of centrioles generates another pair and the twin pairs form the pole of the mitotic spindle.
chromosome, centromeric region The region of a chromosome that includes the centromeric DNA and associated proteins. In monocentric chromosomes, this region corresponds to a single area of the chromosome, whereas in holocentric chromosomes, it is evenly distributed along the chromosome.
condensed chromosome A highly compacted molecule of DNA and associated proteins resulting in a cytologically distinct structure.
cytoplasm The contents of a cell excluding the plasma membrane and nucleus, but including other subcellular structures.
DNA topoisomerase type II (double strand cut, ATP-hydrolyzing) complex Complex that possesses DNA topoisomerase II (double strand cut, ATP-hydrolyzing) activity.
male germ cell nucleus The nucleus of a male germ cell, a reproductive cell in males.
nuclear chromosome A chromosome that encodes the nuclear genome and is found in the nucleus of a eukaryotic cell during the cell cycle phases when the nucleus is intact.
nucleolus A small, dense body one or more of which are present in the nucleus of eukaryotic cells. It is rich in RNA and protein, is not bounded by a limiting membrane, and is not seen during mitosis. Its prime function is the transcription of the nucleolar DNA into 45S ribosomal-precursor RNA, the processing of this RNA into 5.8S, 18S, and 28S components of ribosomal RNA, and the association of these components with 5S RNA and proteins synthesized outside the nucleolus. This association results in the formation of ribonucleoprotein precursors; these pass into the cytoplasm and mature into the 40S and 60S subunits of the ribosome.
nucleoplasm That part of the nuclear content other than the chromosomes or the nucleolus.
nucleus A membrane-bounded organelle of eukaryotic cells in which chromosomes are housed and replicated. In most cells, the nucleus contains all of the cell's chromosomes except the organellar chromosomes, and is the site of RNA synthesis and processing. In some species, or in specialized cell types, RNA metabolism or DNA replication may be absent.
protein-containing complex A stable assembly of two or more macromolecules, i.e. proteins, nucleic acids, carbohydrates or lipids, in which at least one component is a protein and the constituent parts function together.
ribonucleoprotein complex A macromolecular complex that contains both RNA and protein molecules.

16 GO annotations of molecular function

Name Definition
ATP binding Binding to ATP, adenosine 5'-triphosphate, a universally important coenzyme and enzyme regulator.
ATP-dependent activity, acting on DNA Catalytic activity that acts to modify DNA, driven by ATP hydrolysis.
chromatin binding Binding to chromatin, the network of fibers of DNA, protein, and sometimes RNA, that make up the chromosomes of the eukaryotic nucleus during interphase.
DNA binding Any molecular function by which a gene product interacts selectively and non-covalently with DNA (deoxyribonucleic acid).
DNA binding, bending The activity of binding selectively and non-covalently to and distorting the original structure of DNA, typically a straight helix, into a bend, or increasing the bend if the original structure was intrinsically bent due to its sequence.
DNA topoisomerase activity Catalysis of the transient cleavage and passage of individual DNA strands or double helices through one another, resulting a topological transformation in double-stranded DNA.
DNA topoisomerase type II (double strand cut, ATP-hydrolyzing) activity Catalysis of a DNA topological transformation by transiently cleaving a pair of complementary DNA strands to form a gate through which a second double-stranded DNA segment is passed, after which the severed strands in the first DNA segment are rejoined, driven by ATP hydrolysis. The enzyme changes the linking number in multiples of 2.
enzyme binding Binding to an enzyme, a protein with catalytic activity.
histone deacetylase binding Binding to histone deacetylase.
magnesium ion binding Binding to a magnesium (Mg) ion.
protein C-terminus binding Binding to a protein C-terminus, the end of a peptide chain at which the 1-carboxyl function of a constituent amino acid is not attached in peptide linkage to another amino-acid residue.
protein heterodimerization activity Binding to a nonidentical protein to form a heterodimer.
protein homodimerization activity Binding to an identical protein to form a homodimer.
protein kinase C binding Binding to protein kinase C.
sequence-specific DNA binding Binding to DNA of a specific nucleotide composition, e.g. GC-rich DNA binding, or with a specific sequence motif or type of DNA e.g. promotor binding or rDNA binding.
ubiquitin binding Binding to ubiquitin, a protein that when covalently bound to other cellular proteins marks them for proteolytic degradation.

18 GO annotations of biological process

Name Definition
apoptotic chromosome condensation The compaction of chromatin during apoptosis.
cellular response to DNA damage stimulus Any process that results in a change in state or activity of a cell (in terms of movement, secretion, enzyme production, gene expression, etc.) as a result of a stimulus indicating damage to its DNA from environmental insults or errors during metabolism.
chromosome condensation The progressive compaction of dispersed interphase chromatin into threadlike chromosomes prior to mitotic or meiotic nuclear division, or during apoptosis, in eukaryotic cells.
chromosome segregation The process in which genetic material, in the form of chromosomes, is organized into specific structures and then physically separated and apportioned to two or more sets. In eukaryotes, chromosome segregation begins with the condensation of chromosomes, includes chromosome separation, and ends when chromosomes have completed movement to the spindle poles.
DNA ligation The re-formation of a broken phosphodiester bond in the DNA backbone, carried out by DNA ligase.
DNA topological change The process in which a transformation is induced in the topological structure of a double-stranded DNA helix, resulting in a change in linking number.
embryonic cleavage The first few specialized divisions of an activated animal egg.
female meiosis chromosome separation The process in which paired chromosomes are physically detached from each other during female meiosis.
female meiotic nuclear division A cell cycle process by which the cell nucleus divides as part of a meiotic cell cycle in the female germline.
hematopoietic progenitor cell differentiation The process in which precursor cell type acquires the specialized features of a hematopoietic progenitor cell, a class of cell types including myeloid progenitor cells and lymphoid progenitor cells.
negative regulation of DNA duplex unwinding Any process that stops, prevents or reduces the frequency, rate or extent of DNA duplex unwinding.
positive regulation of apoptotic process Any process that activates or increases the frequency, rate or extent of cell death by apoptotic process.
positive regulation of single stranded viral RNA replication via double stranded DNA intermediate Any process that activates or increases the frequency, rate or extent of retroviral genome replication.
positive regulation of transcription by RNA polymerase II Any process that activates or increases the frequency, rate or extent of transcription from an RNA polymerase II promoter.
regulation of circadian rhythm Any process that modulates the frequency, rate or extent of a circadian rhythm. A circadian rhythm is a biological process in an organism that recurs with a regularity of approximately 24 hours.
resolution of meiotic recombination intermediates The cleavage and rejoining of intermediates, such as Holliday junctions, formed during meiotic recombination to produce two intact molecules in which genetic material has been exchanged.
rhythmic process Any process pertinent to the generation and maintenance of rhythms in the physiology of an organism.
sister chromatid segregation The cell cycle process in which sister chromatids are organized and then physically separated and apportioned to two or more sets.

6 homologous proteins in AiPD

UniProt AC Gene Name Protein Name Species Evidence Code
Q02880 TOP2B DNA topoisomerase 2-beta Homo sapiens (Human) PR
P11388 TOP2A DNA topoisomerase 2-alpha Homo sapiens (Human) PR
Q64511 Top2b DNA topoisomerase 2-beta Mus musculus (Mouse) PR
O46374 TOP2A DNA topoisomerase 2-alpha Sus scrofa (Pig) PR
P41516 Top2a DNA topoisomerase 2-alpha Rattus norvegicus (Rat) PR
Q23670 top-2 DNA topoisomerase 2 top-2 Caenorhabditis elegans PR
10 20 30 40 50 60
MELSPLQPVN ENMLMNKKKN EDGKKRLSIE RIYQKKTQLE HILLRPDTYI GSVELVTQQM
70 80 90 100 110 120
WVYDEDVGIN YREVTFVPGL YKIFDEILVN AADNKQRDPK MSCIRVTIDP ENNVISIWNN
130 140 150 160 170 180
GKGIPVVEHK VEKIYVPALI FGQLLTSSNY DDDEKKVTGG RNGYGAKLCN IFSTKFTVET
190 200 210 220 230 240
ASREYKKMFK QTWMDNMGRA GDMELKPFSG EDYTCITFQP DLSKFKMQSL DKDIVALMVR
250 260 270 280 290 300
RAYDIAGSTK DVKVFLNGNS LPVKGFRSYV DLYLKDKVDE TGNSLKVIHE QVNPRWEVCL
310 320 330 340 350 360
TMSERGFQQI SFVNSIATSK GGRHVDYVAD QIVSKLVDVV KKKNKGGVAV KAHQVKNHMW
370 380 390 400 410 420
IFVNALIENP TFDSQTKENM TLQAKSFGST CQLSEKFIKA AIGCGIVESI LNWVKFKAQI
430 440 450 460 470 480
QLNKKCSAVK HTKIKGIPKL DDANDAGSRN STECTLILTE GDSAKTLAVS GLGVVGRDKY
490 500 510 520 530 540
GVFPLRGKIL NVREASHKQI MENAEINNII KIVGLQYKKN YEDEDSLKTL RYGKIMIMTD
550 560 570 580 590 600
QDQDGSHIKG LLINFIHHNW PSLLRHRFLE EFITPIVKVS KNKQEIAFYS LPEFEEWKSS
610 620 630 640 650 660
TPNHKKWKVK YYKGLGTSTS KEAKEYFADM KRHRIQFKYS GPEDDAAISL AFSKKQVDDR
670 680 690 700 710 720
KEWLTNFMED RRQRKLLGLP EDYLYGQSTS YLTYNDFINK ELILFSNSDN ERSIPSMVDG
730 740 750 760 770 780
LKPGQRKVLF TCFKRNDKRE VKVAQLAGSV AEMSSYHHGE MSLMMTIINL AQNFVGSNNL
790 800 810 820 830 840
NLLQPIGQFG TRLHGGKDSA SPRYIFTMLS PLARLLFPPK DDHTLRFLYD DNQRVEPEWY
850 860 870 880 890 900
IPIIPMVLIN GAEGIGTGWS CKIPNFDVRE VVNNIRRLLD GEEPLPMLPS YKNFKGTIEE
910 920 930 940 950 960
LASNQYVING EVAILDSTTI EISELPIRTW TQTYKEQVLE PMLNGTEKTP SLITDYREYH
970 980 990 1000 1010 1020
TDTTVKFVIK MTEEKLAEAE RVGLHKVFKL QSSLTCNSMV LFDHVGCLKK YDTVLDILRD
1030 1040 1050 1060 1070 1080
FFELRLKYYG LRKEWLLGML GAESSKLNNQ ARFILEKIDG KIVIENKPKK ELIKVLIQRG
1090 1100 1110 1120 1130 1140
YDSDPVKAWK EAQQKVPDEE ENEESDTETS TSDSAAEAGP TFNYLLDMPL WYLTKEKKDE
1150 1160 1170 1180 1190 1200
LCKQRNEKEQ ELNTLKQKSP SDLWKEDLAV FIEELEVVEA KEKQDEQVGL PGKAGKAKGK
1210 1220 1230 1240 1250 1260
KAQMCADVLP SPRGKRVIPQ VTVEMKAEAE KKIRKKIKSE NVEGTPAEDG AEPGSLRQRI
1270 1280 1290 1300 1310 1320
EKKQKKEPGA KKQTTLPFKP VKKGRKKNPW SDSESDVSSN ESNVDVPPRQ KEQRSAAAKA
1330 1340 1350 1360 1370 1380
KFTVDLDSDE DFSGLDEKDE DEDFLPLDAT PPKAKIPPKN TKKALKTQGS SMSVVDLESD
1390 1400 1410 1420 1430 1440
VKDSVPASPG VPAADFPAET EQSKPSKKTV GVKKTATKSQ SSVSTAGTKK RAAPKGTKSD
1450 1460 1470 1480 1490 1500
SALSARVSEK PAPAKAKNSR KRKPSSSDSS DSDFERAISK GATSKKAKGE EQDFPVDLED
1510 1520
TIAPRAKSDR ARKPIKYLEE SDDDDDLF