Q12380
PR
Gene name |
ATG5 (APG5, YPL149W, P2601) |
Protein name |
Autophagy protein 5 |
Names |
|
Species |
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
KEGG Pathway |
sce:YPL149W |
EC number |
|
Protein Class |
|
Descriptions
The autoinhibited protein was predicted that may have potential autoinhibitory elements via cis-regPred.
Autoinhibitory domains (AIDs)
Target domain |
|
Relief mechanism |
|
Assay |
cis-regPred |
Accessory elements
No accessory elements
Autoinhibited structure
Activated structure
4 structures for Q12380
| Entry ID | Method | Resolution | Chain | Position | Source |
|---|---|---|---|---|---|
| 2DYM | X-ray | 220 A | A/C/E/G | 1-294 | PDB |
| 2DYO | X-ray | 197 A | A | 1-294 | PDB |
| 3W1S | X-ray | 260 A | A | 1-284 | PDB |
| AF-Q12380-F1 | Predicted | AlphaFoldDB |
6 variants for Q12380
| Variant ID(s) | Position | Change | Description | Diseaes Association | Provenance |
|---|---|---|---|---|---|
| s16-271459 | 51 | L>F | No | SGRP | |
| s16-271511 | 68 | S>N | No | SGRP | |
| s16-271943 | 212 | T>I | No | SGRP | |
| s16-271970 | 221 | S>T | No | SGRP | |
| s16-271991 | 228 | T>M | No | SGRP | |
| s16-272092 | 262 | L>V | No | SGRP |
No associated diseases with Q12380
No regional properties for Q12380
| Type | Name | Position | InterPro Accession |
|---|---|---|---|
| No domain, repeats, and functional sites for Q12380 | |||
Functions
10 GO annotations of cellular component
| Name | Definition |
|---|---|
| Atg12-Atg5-Atg16 complex | A protein complex required for the expansion of the autophagosomal membrane. In budding yeast, this complex consists of Atg12p, Atg5p and Atg16p. |
| autophagosome | A double-membrane-bounded compartment that engulfs endogenous cellular material as well as invading microorganisms to target them to the lytic vacuole/lysosome for degradation as part of macroautophagy. |
| cytosol | The part of the cytoplasm that does not contain organelles but which does contain other particulate matter, such as protein complexes. |
| membrane | A lipid bilayer along with all the proteins and protein complexes embedded in it an attached to it. |
| nucleus | A membrane-bounded organelle of eukaryotic cells in which chromosomes are housed and replicated. In most cells, the nucleus contains all of the cell's chromosomes except the organellar chromosomes, and is the site of RNA synthesis and processing. In some species, or in specialized cell types, RNA metabolism or DNA replication may be absent. |
| phagophore | A disk-like structure that expands, rounds up into a cup-shaped structure, and eventually closes around its cargo (for example cytoplasmic components) to become an autophagosome or Cvt vesicle. |
| phagophore assembly site | Punctate structures proximal to the endoplasmic reticulum which are the sites where the Atg machinery assembles upon autophagy induction. |
| phagophore assembly site membrane | A cellular membrane associated with the phagophore assembly site. |
| transferase complex | A protein complex capable of catalyzing the transfer of a group, e.g. a methyl group, glycosyl group, acyl group, phosphorus-containing, or other groups, from one compound (generally regarded as the donor) to another compound (generally regarded as the acceptor). |
| vacuole-isolation membrane contact site | An organelle membrane contact site formed at the junction of the vacuolar membrane and the isolation membrane or phagophore in response to starvation or other stresses, leading to the formation of the autophagosome. |
3 GO annotations of molecular function
| Name | Definition |
|---|---|
| cargo receptor ligand activity | The activity of a gene product that interacts with a cargo receptor and initiates endocytosis. |
| enzyme activator activity | Binds to and increases the activity of an enzyme. |
| hydrolase activity | Catalysis of the hydrolysis of various bonds, e.g. C-O, C-N, C-C, phosphoric anhydride bonds, etc. |
11 GO annotations of biological process
| Name | Definition |
|---|---|
| autophagosome assembly | The formation of a double membrane-bounded structure, the autophagosome, that occurs when a specialized membrane sac, called the isolation membrane, starts to enclose a portion of the cytoplasm. |
| autophagy of mitochondrion | The autophagic process in which mitochondria are delivered to a type of vacuole and degraded in response to changing cellular conditions. |
| autophagy of nucleus | A form of autophagy, by which damaged or non-essential parts of the nucleus, or even an entire nucleus is degraded. |
| C-terminal protein lipidation | The covalent attachment of a lipid group to the carboxy-terminus of a protein. |
| cellular response to nitrogen starvation | Any process that results in a change in state or activity of a cell (in terms of movement, secretion, enzyme production, gene expression, etc.) as a result of deprivation of nitrogen. |
| cytoplasm to vacuole transport by the Cvt pathway | A cytoplasm to vacuole targeting pathway that uses machinery common with autophagy. The Cvt vesicle is formed when the receptor protein, Atg19, binds to the complexes of the target protein (aminopeptidase or alpha-mannosidase homododecamers), forming the Cvt complex. Atg11 binds to Atg9 and transports the Cvt complex to the pre-autophagosome (PAS). The phagophore membrane expands around the Cvt complex (excluding bulk cytoplasm) forming the Cvt vesicle. This pathway is mostly observed in yeast. |
| late nucleophagy | A type of nucleophagy, distinct from piecemeal microautophagy of the nucleus (PNM) where the nuclear material is delivered to the vacuole/lysosome for breakdown and recycling later than observed for PNM. |
| macroautophagy | The major inducible pathway for the general turnover of cytoplasmic constituents in eukaryotic cells, it is also responsible for the degradation of active cytoplasmic enzymes and organelles during nutrient starvation. Macroautophagy involves the formation of double-membrane-bounded autophagosomes which enclose the cytoplasmic constituent targeted for degradation in a membrane-bounded structure. Autophagosomes then fuse with a lysosome (or vacuole) releasing single-membrane-bounded autophagic bodies that are then degraded within the lysosome (or vacuole). Some types of macroautophagy, e.g. pexophagy, mitophagy, involve selective targeting of the targets to be degraded. |
| piecemeal microautophagy of the nucleus | Degradation of a cell nucleus by lysosomal microautophagy. |
| protein lipidation | The covalent attachment of lipid groups to an amino acid in a protein. |
| selective autophagy | The macroautophagy process in which specific structures are targeted by the autophagy process. |
6 homologous proteins in AiPD
| UniProt AC | Gene Name | Protein Name | Species | Evidence Code |
|---|---|---|---|---|
| Q3MQ24 | ATG5 | Autophagy protein 5 | Bos taurus (Bovine) | PR |
| Q9H1Y0 | ATG5 | Autophagy protein 5 | Homo sapiens (Human) | PR |
| Q99J83 | Atg5 | Autophagy protein 5 | Mus musculus (Mouse) | PR |
| Q3MQ04 | ATG5 | Autophagy protein 5 | Sus scrofa (Pig) | PR |
| Q3MQ06 | Atg5 | Autophagy protein 5 | Rattus norvegicus (Rat) | PR |
| Q9FFI2 | ATG5 | Autophagy protein 5 | Arabidopsis thaliana (Mouse-ear cress) | PR |
| 10 | 20 | 30 | 40 | 50 | 60 |
| MNDIKQLLWN | GELNVLVSID | PSFLMKGSPR | EIAVLRIRVP | RETYLVNYMP | LIWNKIKSFL |
| 70 | 80 | 90 | 100 | 110 | 120 |
| SFDPLTDSEK | YFWFEHNKTP | IPWNYPVGVL | FDCLAGKSAT | FTTSFENQVK | DVLTFLRIHL |
| 130 | 140 | 150 | 160 | 170 | 180 |
| VMGDSLPPTI | IPIASSKTQA | EKFWFHQWKQ | VCFILNGSSK | AIMSLSVNEA | RKFWGSVITR |
| 190 | 200 | 210 | 220 | 230 | 240 |
| NFQDFIEISN | KISSSRPRHI | PLIIQTSRTS | GTFRISQPTI | SMTGVNPTLK | DIEGDILDVK |
| 250 | 260 | 270 | 280 | 290 | |
| EGINGNDVMV | ICQGIEIPWH | MLLYDLYSKL | RSFDGFLYIT | LVPIKGGDKA | SSEL |