P28240

PR
Gene name
ICL1
Protein name
Isocitrate lyase
Names
ICL, Methylisocitrate lyase, MICA, Threo-D(S)-isocitrate glyoxylate-lyase
Species
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
KEGG Pathway
sce:YER065C
EC number
4.1.3.1: Oxo-acid-lyases
Protein Class

Descriptions

The autoinhibited protein was predicted that may have potential autoinhibitory elements via cis-regPred.

Autoinhibitory domains (AIDs)

1-78 (Predicted disordered autoinhibitory region) PR

Target domain

Relief mechanism

Assay

cis-regPred

Accessory elements

No accessory elements

References

Autoinhibited structure

Activated structure

2 structures for P28240

Entry ID Method Resolution Chain Position Source
7EBC X-ray 230 A A/B/C/D 1-557 PDB
AF-P28240-F1 Predicted AlphaFoldDB

3 variants for P28240

Variant ID(s) Position Change Description Diseaes Association Provenance
s05-286439 158 K>N No SGRP
s05-286036 293 A>T No SGRP
s05-285736 393 I>V No SGRP

No associated diseases with P28240

2 regional properties for P28240

Type Name Position InterPro Accession
conserved_site Isocitrate lyase/phosphorylmutase, conserved site 215 - 220 IPR018523
domain ICL/PEPM domain 76 - 488 IPR039556

Functions

Description
EC Number 4.1.3.1 Oxo-acid-lyases
Subcellular Localization
  • Cytoplasm
  • Secreted, extracellular space, extracellular matrix
  • Vacuole
  • Localizes in extracellular vesicles during growth on lown glucose
  • When glucose is added to glucose-starved wild-type cells, levels of extracellular ICL1 get reduced
  • Targeted to the vacuole for degradation in 3-day-starved cells
PANTHER Family
PANTHER Subfamily
PANTHER Protein Class
PANTHER Pathway Category No pathway information available

3 GO annotations of cellular component

Name Definition
cytoplasm The contents of a cell excluding the plasma membrane and nucleus, but including other subcellular structures.
extracellular region The space external to the outermost structure of a cell. For cells without external protective or external encapsulating structures this refers to space outside of the plasma membrane. This term covers the host cell environment outside an intracellular parasite.
vacuole A closed structure, found only in eukaryotic cells, that is completely surrounded by unit membrane and contains liquid material. Cells contain one or several vacuoles, that may have different functions from each other. Vacuoles have a diverse array of functions. They can act as a storage organelle for nutrients or waste products, as a degradative compartment, as a cost-effective way of increasing cell size, and as a homeostatic regulator controlling both turgor pressure and pH of the cytosol.

3 GO annotations of molecular function

Name Definition
isocitrate lyase activity Catalysis of the reaction: isocitrate = glyoxylate + succinate.
metal ion binding Binding to a metal ion.
methylisocitrate lyase activity Catalysis of the reaction: (2S,3R)-3-hydroxybutane-1,2,3-tricarboxylate = pyruvate + succinate.

2 GO annotations of biological process

Name Definition
glyoxylate cycle A modification of the TCA cycle occurring in some plants and microorganisms, in which isocitrate is cleaved to glyoxylate and succinate. Glyoxylate can then react with acetyl-CoA to form malate.
tricarboxylic acid cycle A nearly universal metabolic pathway in which the acetyl group of acetyl coenzyme A is effectively oxidized to two CO2 and four pairs of electrons are transferred to coenzymes. The acetyl group combines with oxaloacetate to form citrate, which undergoes successive transformations to isocitrate, 2-oxoglutarate, succinyl-CoA, succinate, fumarate, malate, and oxaloacetate again, thus completing the cycle. In eukaryotes the tricarboxylic acid is confined to the mitochondria. See also glyoxylate cycle.

8 homologous proteins in AiPD

UniProt AC Gene Name Protein Name Species Evidence Code
Q12031 ICL2 Mitochondrial 2-methylisocitrate lyase ICL2 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) PR
P17069 Isocitrate lyase Gossypium hirsutum (Upland cotton) (Gossypium mexicanum) PR
Q6Z6M4 ICL Isocitrate lyase Oryza sativa subsp japonica (Rice) PR
Q10663 icl-1 Bifunctional glyoxylate cycle protein Caenorhabditis elegans PR
P45457 ICL2 Isocitrate lyase 2 Glycine max (Soybean) (Glycine hispida) PR
P45456 ICL1 Isocitrate lyase 1 Glycine max (Soybean) (Glycine hispida) PR
P28297 ICL Isocitrate lyase Arabidopsis thaliana (Mouse-ear cress) PR
P49297 Isocitrate lyase Solanum lycopersicum (Tomato) (Lycopersicon esculentum) PR
10 20 30 40 50 60
MPIPVGNTKN DFAALQAKLD ADAAEIEKWW SDSRWSKTKR NYSARDIAVR RGTFPPIEYP
70 80 90 100 110 120
SSVMARKLFK VLEKHHNEGT VSKTFGALDP VQISQMAKYL DTIYISGWQC SSTASTSNEP
130 140 150 160 170 180
GPDLADYPMD TVPNKVEHLF KAQLFHDRKQ LEARSKAKSQ EELDEMGAPI DYLTPIVADA
190 200 210 220 230 240
DAGHGGLTAV FKLTKMFIER GAAGIHMEDQ TSTNKKCGHM AGRCVIPVQE HVNRLVTIRM
250 260 270 280 290 300
CADIMHSDLI VVARTDSEAA TLISSTIDTR DHYFIVGATN PNIEPFAEVL NDAIMSGASG
310 320 330 340 350 360
QELADIEQKW CRDAGLKLFH EAVIDEIERS ALSNKQELIK KFTSKVGPLT ETSHREAKKL
370 380 390 400 410 420
AKEILGHEIF FDWELPRVRE GLYRYRGGTQ CSIMRARAFA PYADLVWMES NYPDFQQAKE
430 440 450 460 470 480
FAEGVKEKFP DQWLAYNLSP SFNWPKAMSV DEQHTFIQRL GDLGYIWQFI TLAGLHTNAL
490 500 510 520 530 540
AVHNFSRDFA KDGMKAYAQN VQQREMDDGV DVLKHQKWSG AEYIDGLLKL AQGGVSATAA
550
MGTGVTEDQF KENGVKK