P24135

SS
Gene name
Plcg2
Protein name
1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase gamma-2
Names
EC 3.1.4.11 , Phosphoinositide phospholipase C-gamma-2 , Phospholipase C-IV , PLC-IV , Phospholipase C-gamma-2 , PLC-gamma-2
Species
Rattus norvegicus (Rat)
KEGG Pathway
rno:29337
EC number
3.1.4.11: Phosphoric diester hydrolases
Protein Class

Descriptions

Phospholipase C gamma 1 (PLCγ1) is an enzyme that plays a crucial role in intracellular signaling pathways. It hydrolyzes phosphatidylinositol 4,5-bisphosphate (PIP2) to generate second messengers. The autoinhibition of PLCγ1 is mediated by the interaction of its sPH and cSH2 domains, which form extensive autoinhibitory contacts with the core of PLCγ1, thereby blocking membrane engagement and access to the PIP2 substrate. Autoinhibition is relieved by phosphorylation at Y783.

Autoinhibitory domains (AIDs)

475-509 (sPH domain, first part) SS

Target domain

312-456 (Catalytic PLC X domain)

Relief mechanism

PTM, Partner binding

Assay

872-908 (sPH domain, second part) SS

Target domain

312-456 (Catalytic PLC X domain)

Relief mechanism

PTM, Partner binding

Assay

646-735 (cSH2 domain) SS

Target domain

312-456 (Catalytic PLC X domain)

Relief mechanism

PTM, Partner binding

Assay

Accessory elements

No accessory elements

References

Autoinhibited structure

Activated structure

2 structures for P24135

Entry ID Method Resolution Chain Position Source
2EOB NMR - A 633-743 PDB
AF-P24135-F1 Predicted AlphaFoldDB

No variants for P24135

Variant ID(s) Position Change Description Diseaes Association Provenance
No variants for P24135

No associated diseases with P24135

4 regional properties for P24135

Type Name Position InterPro Accession
domain C2 domain 430 - 560 IPR000008
domain Phosphatidylinositol-specific phospholipase C, X domain 112 - 256 IPR000909
domain Phospholipase C, phosphatidylinositol-specific, Y domain 313 - 430 IPR001711
domain Phosphoinositide-specific phospholipase C, EF-hand-like domain 27 - 102 IPR015359

Functions

Description
EC Number 3.1.4.11 Phosphoric diester hydrolases
Subcellular Localization
  • Membrane raft
PANTHER Family
PANTHER Subfamily
PANTHER Protein Class
PANTHER Pathway Category No pathway information available

7 GO annotations of cellular component

Name Definition
cytoplasm The contents of a cell excluding the plasma membrane and nucleus, but including other subcellular structures.
cytosol The part of the cytoplasm that does not contain organelles but which does contain other particulate matter, such as protein complexes.
intracellular vesicle Any vesicle that is part of the intracellular region.
membrane raft Any of the small (10-200 nm), heterogeneous, highly dynamic, sterol- and sphingolipid-enriched membrane domains that compartmentalize cellular processes. Small rafts can sometimes be stabilized to form larger platforms through protein-protein and protein-lipid interactions.
perinuclear region of cytoplasm Cytoplasm situated near, or occurring around, the nucleus.
plasma membrane The membrane surrounding a cell that separates the cell from its external environment. It consists of a phospholipid bilayer and associated proteins.
ruffle membrane The portion of the plasma membrane surrounding a ruffle.

6 GO annotations of molecular function

Name Definition
phosphatidylinositol phospholipase C activity Catalysis of the reaction
phosphorylation-dependent protein binding Binding to a protein upon phosphorylation of the target protein.
phosphotyrosine residue binding Binding to a phosphorylated tyrosine residue within a protein.
protein kinase binding Binding to a protein kinase, any enzyme that catalyzes the transfer of a phosphate group, usually from ATP, to a protein substrate.
protein tyrosine kinase binding Binding to protein tyrosine kinase.
scaffold protein binding Binding to a scaffold protein. Scaffold proteins are crucial regulators of many key signaling pathways. Although not strictly defined in function, they are known to interact and/or bind with multiple members of a signaling pathway, tethering them into complexes.

55 GO annotations of biological process

Name Definition
antifungal innate immune response An defense response against a fungus mediated through an innate immune response. An innate immune response is mediated by germline encoded components that directly recognize components of potential pathogens.
B cell differentiation The process in which a precursor cell type acquires the specialized features of a B cell. A B cell is a lymphocyte of B lineage with the phenotype CD19-positive and capable of B cell mediated immunity.
B cell receptor signaling pathway The series of molecular signals initiated by the cross-linking of an antigen receptor on a B cell.
calcium-mediated signaling Any intracellular signal transduction in which the signal is passed on within the cell via calcium ions.
cell activation A multicellular organismal process by which exposure to an activating factor such as a cellular or soluble ligand results in a change in the morphology or behavior of a cell.
cellular response to calcium ion Any process that results in a change in state or activity of a cell (in terms of movement, secretion, enzyme production, gene expression, etc.) as a result of a calcium ion stimulus.
cellular response to lectin Any process that results in a change in state or activity of a cell (in terms of movement, secretion, enzyme production, gene expression, etc.) as a result of a lectin stimulus. A lectin is a carbohydrate-binding protein, highly specific for binding sugar moieties.
cellular response to lipid Any process that results in a change in state or activity of a cell (in terms of movement, secretion, enzyme production, gene expression, etc.) as a result of a lipid stimulus.
follicular B cell differentiation The process in which a B cell in the spleen acquires the specialized features of a follicular B cell. Follicular B cells are major population of mature recirculating B cells in the spleen and are located in the B-cell follicle region.
inositol trisphosphate biosynthetic process The chemical reactions and pathways resulting in the formation of inositol trisphosphate, 1,2,3,4,5,6-cyclohexanehexol, with three phosphate groups attached.
intracellular signal transduction The process in which a signal is passed on to downstream components within the cell, which become activated themselves to further propagate the signal and finally trigger a change in the function or state of the cell.
lipopolysaccharide-mediated signaling pathway The series of molecular signals initiated by the binding of a lipopolysaccharide (LPS) to a receptor on the surface of a target cell, and ending with the regulation of a downstream cellular process, e.g. transcription. Lipopolysaccharides are major components of the outer membrane of Gram-negative bacteria, making them prime targets for recognition by the immune system.
macrophage activation involved in immune response A change in morphology and behavior of a macrophage resulting from exposure to a cytokine, chemokine, cellular ligand, or soluble factor, leading to the initiation or perpetuation of an immune response.
negative regulation of programmed cell death Any process that stops, prevents, or reduces the frequency, rate or extent of programmed cell death, cell death resulting from activation of endogenous cellular processes.
phosphatidylinositol biosynthetic process The chemical reactions and pathways resulting in the formation of phosphatidylinositol, any glycophospholipid in which the sn-glycerol 3-phosphate residue is esterified to the 1-hydroxyl group of 1D-myo-inositol.
phosphatidylinositol metabolic process The chemical reactions and pathways involving phosphatidylinositol, any glycophospholipid in which a sn-glycerol 3-phosphate residue is esterified to the 1-hydroxyl group of 1D-myo-inositol.
phosphatidylinositol-mediated signaling The series of molecular signals in which a cell uses a phosphatidylinositol-mediated signaling to convert a signal into a response. Phosphatidylinositols include phosphatidylinositol (PtdIns) and its phosphorylated derivatives.
phospholipid catabolic process The chemical reactions and pathways resulting in the breakdown of phospholipids, any lipid containing phosphoric acid as a mono- or diester.
positive regulation of calcium-mediated signaling Any process that activates or increases the frequency, rate or extent of calcium-mediated signaling.
positive regulation of cell cycle G1/S phase transition Any signalling pathway that activates or increases the activity of a cell cycle cyclin-dependent protein kinase to modulate the switch from G1 phase to S phase of the cell cycle.
positive regulation of cytosolic calcium ion concentration Any process that increases the concentration of calcium ions in the cytosol.
positive regulation of dendritic cell cytokine production Any process that activates or increases the frequency, rate, or extent of dendritic cell cytokine production.
positive regulation of DNA-binding transcription factor activity Any process that activates or increases the frequency, rate or extent of activity of a transcription factor, any factor involved in the initiation or regulation of transcription.
positive regulation of epithelial cell migration Any process that activates or increases the frequency, rate or extent of epithelial cell migration.
positive regulation of gene expression Any process that increases the frequency, rate or extent of gene expression. Gene expression is the process in which a gene's coding sequence is converted into a mature gene product (protein or RNA).
positive regulation of I-kappaB phosphorylation Any process that activates or increases the frequency, rate or extent of I-kappaB phosphorylation.
positive regulation of interleukin-10 production Any process that activates or increases the frequency, rate, or extent of interleukin-10 production.
positive regulation of interleukin-12 production Any process that activates or increases the frequency, rate, or extent of interleukin-12 production.
positive regulation of interleukin-2 production Any process that activates or increases the frequency, rate, or extent of interleukin-2 production.
positive regulation of interleukin-23 production Any process that activates or increases the frequency, rate, or extent of interleukin-23 production.
positive regulation of interleukin-6 production Any process that activates or increases the frequency, rate, or extent of interleukin-6 production.
positive regulation of macrophage cytokine production Any process that increases the rate, frequency or extent of macrophage cytokine production. Macrophage cytokine production is the appearance of a chemokine due to biosynthesis or secretion following a cellular stimulus, resulting in an increase in its intracellular or extracellular levels.
positive regulation of MAPK cascade Any process that activates or increases the frequency, rate or extent of signal transduction mediated by the MAPK cascade.
positive regulation of neuroinflammatory response Any process that activates or increases the frequency, rate or extent of neuroinflammatory response.
positive regulation of NLRP3 inflammasome complex assembly Any process that activates or increases the frequency, rate or extent of NLRP3 inflammasome complex assembly.
positive regulation of peptidyl-tyrosine phosphorylation Any process that activates or increases the frequency, rate or extent of the phosphorylation of peptidyl-tyrosine.
positive regulation of phagocytosis, engulfment Any process that activates or increases the frequency, rate or extent of the internalization of bacteria, immune complexes and other particulate matter or of an apoptotic cell by phagocytosis.
positive regulation of reactive oxygen species biosynthetic process Any process that activates or increases the frequency, rate or extent of reactive oxygen species biosynthetic process.
positive regulation of receptor internalization Any process that activates or increases the frequency, rate or extent of receptor internalization.
positive regulation of tumor necrosis factor production Any process that activates or increases the frequency, rate or extent of tumor necrosis factor production.
positive regulation of type I interferon production Any process that activates or increases the frequency, rate, or extent of type I interferon production. Type I interferons include the interferon-alpha, beta, delta, episilon, zeta, kappa, tau, and omega gene families.
programmed cell death A process which begins when a cell receives an internal or external signal and activates a series of biochemical events (signaling pathway). The process ends with the death of the cell.
regulation of calcineurin-NFAT signaling cascade Any process that modulates the frequency, rate or extent of the calcineurin-NFAT signaling cascade.
regulation of canonical NF-kappaB signal transduction Any process that modulates I-kappaB kinase/NF-kappaB signaling.
regulation of gene expression Any process that modulates the frequency, rate or extent of gene expression. Gene expression is the process in which a gene's coding sequence is converted into a mature gene product (protein or RNA).
regulation of lipid metabolic process Any process that modulates the frequency, rate or extent of the chemical reactions and pathways involving lipids.
release of sequestered calcium ion into cytosol The process in which calcium ions sequestered in the endoplasmic reticulum, Golgi apparatus or mitochondria are released into the cytosolic compartment.
response to ATP Any process that results in a change in state or activity of a cell or an organism (in terms of movement, secretion, enzyme production, gene expression, etc.) as a result of an ATP (adenosine 5'-triphosphate) stimulus.
response to axon injury Any process that results in a change in state or activity of a cell or an organism (in terms of movement, secretion, enzyme production, gene expression, etc.) as a result of an axon injury stimulus.
response to lipopolysaccharide Any process that results in a change in state or activity of an organism (in terms of movement, secretion, enzyme production, gene expression, etc.) as a result of a lipopolysaccharide stimulus; lipopolysaccharide is a major component of the cell wall of gram-negative bacteria.
response to magnesium ion Any process that results in a change in state or activity of a cell or an organism (in terms of movement, secretion, enzyme production, gene expression, etc.) as a result of a magnesium ion stimulus.
response to yeast Any process that results in a change in state or activity of a cell or an organism (in terms of movement, secretion, enzyme production, gene expression, etc.) as a result of a stimulus from a yeast species.
stimulatory C-type lectin receptor signaling pathway The series of molecular signals initiated by the binding of C-type lectin to its receptor on the surface of a target cell, and resulting in cellular activation.
T cell receptor signaling pathway The series of molecular signals initiated by the cross-linking of an antigen receptor on a T cell.
toll-like receptor signaling pathway The series of molecular signals initiated by a ligand binding to a toll-like receptor of a target cell. Toll-like receptors directly bind pattern motifs from a variety of microbial sources to initiate an innate immune response.

17 homologous proteins in AiPD

UniProt AC Gene Name Protein Name Species Evidence Code
P08487 PLCG1 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase gamma-1 Bos taurus (Bovine) EV SS
P19174 PLCG1 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase gamma-1 Homo sapiens (Human) EV
P16885 PLCG2 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase gamma-2 Homo sapiens (Human) SS
Q62077 Plcg1 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase gamma-1 Mus musculus (Mouse) SS
P10686 Plcg1 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase gamma-1 Rattus norvegicus (Rat) EV
O89040 Plcb2 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-2 Rattus norvegicus (Rat) PR
P10687 Plcb1 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-1 Rattus norvegicus (Rat) SS
Q99P84 Plce1 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase epsilon-1 Rattus norvegicus (Rat) EV
P10688 Plcd1 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase delta-1 Rattus norvegicus (Rat) SS
Q99JE6 Plcb3 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-3 Rattus norvegicus (Rat) SS
Q39032 PLC1 Phosphoinositide phospholipase C 1 Arabidopsis thaliana (Mouse-ear cress) PR
Q56W08 PLC3 Phosphoinositide phospholipase C 3 Arabidopsis thaliana (Mouse-ear cress) PR
Q6NMA7 PLC9 Phosphoinositide phospholipase C 9 Arabidopsis thaliana (Mouse-ear cress) PR
Q8GV43 PLC6 Phosphoinositide phospholipase C 6 Arabidopsis thaliana (Mouse-ear cress) PR
Q944C2 PLC5 Phosphoinositide phospholipase C 5 Arabidopsis thaliana (Mouse-ear cress) PR
Q9STZ3 PLC8 Phosphoinositide phospholipase C 8 Arabidopsis thaliana (Mouse-ear cress) PR
Q944C1 PLC4 Phosphoinositide phospholipase C 4 Arabidopsis thaliana (Mouse-ear cress) PR
10 20 30 40 50 60
MTTMVNVDTL PEYEKSQIKR ALELGTVMTV FSARKSTPER RTVQMIMETR QVAWSKTADK
70 80 90 100 110 120
IEGFLDIMEI KEIRPGKNSK DFERAKAVRH KADCCFTIFY GTQFVLSTLS LATDSKEDAV
130 140 150 160 170 180
KWLSGLKILH QEAMNASTPT MIESWLRKQI YSVDQTRRNS ISLRELKTIL PLVNFKVSGI
190 200 210 220 230 240
KFLKDKLVEI GAQKDELSFE QFHLFYKKLM FEQQKSILDE FKKDSSVFIL GNTDRPDASA
250 260 270 280 290 300
VYLQDFQRFL LHEQQELWAQ DLNKVRERMT KFIDDTMRET AEPFLFVDEF LTYLFSRENS
310 320 330 340 350 360
IWDEKYDAVD MQDMNNPLSH YWISSSHNTY LTGDQLRSES STEAYIRCLR AGCRCIELDC
370 380 390 400 410 420
WDGPDGKPII YHGWTRTTKI KFDDVVQAIR DHAFVTSSFP VILSIEEHCS VEQQRHMAKV
430 440 450 460 470 480
FKEVLGDLLL TKPTEASADQ LPSPSQLREK IIIKHKKLGP RGDVDVNVED KKDEHKTQGE
490 500 510 520 530 540
LYMWDSIDQK WTRHYCAIAD AKLSFSDDIE QTVEEDPVQD TPPTELHFGE KWFHKKVESR
550 560 570 580 590 600
TSAEKLLQEY CAETGAKDGT FLVRESETFP NDYTLSFWRS GRVQHCRIRS TMEGGVMKYY
610 620 630 640 650 660
LTDNLTFNSI YALIQHYREA HLRCAEFELR LTDPVPNPNP HESKPWYYDR LSRGEAEDML
670 680 690 700 710 720
MRIPRDGAFL IRKREGTDSY AITFRARGKV KHCRINRDGR HFVLGTSAYF ESLVELVSYY
730 740 750 760 770 780
EKHALYRKMR LRYPVTPELL ERYNMERDIN SLYDVSRMYV DPSEINPSMP QRTVKALYDY
790 800 810 820 830 840
KAKRSDELTF CRGALIHNVS KEPGGWWKGD YGTRIQQYFP SNYVEDISAG DAEEMEKQII
850 860 870 880 890 900
EDNPLGSLCR GILDLNTYNV VKAPQGKNQK AFVFILEPKK QGDPPVEFAT DRVEELFEWF
910 920 930 940 950 960
QSIREITWKI DTKENNMKYW ERNQSIAIEL SDLVVYCKPT SKTKDHLENP DFREIRSFVE
970 980 990 1000 1010 1020
TKADSIVRQK PVDLLRYNQK GLTRVYPKGQ RVDSSNYDPF RLWLCGSQMV ALNFQTPDKY
1030 1040 1050 1060 1070 1080
MQMNHALFSL NGRTGYVLQP ESMRSEKYDP MPPESQRKIL MTLTVKVLGA RHLPKLGRSI
1090 1100 1110 1120 1130 1140
ACPFVEVEIC GAEYDSNKFK TTVVNDNGLS PVWAPTQEKV TFEIYDPNLA FLRFLVYEED
1150 1160 1170 1180 1190 1200
MFSDPNFLAH ATYPIKGIKS GFRSVPLKNG YSEDIELASL LVFCEMRPVL ESEEELYSSC
1210 1220 1230 1240 1250 1260
RQLRRRQEEL NNQLFLYDTH QNLRGANRDA LVKEFNVNEN QLRLYQEKCN RRLREKRVSN
SRFYS