P15938

PR
Gene name
PRP16 (YKR086W, YKR406)
Protein name
Pre-mRNA-splicing factor ATP-dependent RNA helicase PRP16
Names
Species
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
KEGG Pathway
sce:YKR086W
EC number
3.6.4.13: Acting on ATP; involved in cellular and subcellular movement
Protein Class

Descriptions

The autoinhibited protein was predicted that may have potential autoinhibitory elements via cis-regPred.

Autoinhibitory domains (AIDs)

88-181 (Predicted disordered autoinhibitory region) PR

Target domain

Relief mechanism

Assay

cis-regPred

Accessory elements

No accessory elements

References

Autoinhibited structure

Activated structure

3 structures for P15938

Entry ID Method Resolution Chain Position Source
5LJ5 EM 380 A Q 1-1071 PDB
5WSG EM 400 A e 1-1071 PDB
AF-P15938-F1 Predicted AlphaFoldDB

15 variants for P15938

Variant ID(s) Position Change Description Diseaes Association Provenance
s11-599542 15 K>R No SGRP
s11-599550 18 T>S No SGRP
s11-599595 33 L>V No SGRP
s11-600048 184 L>V No SGRP
s11-600178 227 E>G No SGRP
s11-600567 357 S>P No SGRP
s11-600731 411 I>M No SGRP
s11-600795 433 I>V No SGRP
s11-600819 441 S>A No SGRP
s11-600906 470 V>I No SGRP
s11-601799 767 I>M No SGRP
s11-601808 770 S>R No SGRP
s11-601957 820 R>K No SGRP
s11-602568 1024 D>N No SGRP
s11-602662 1055 N>T No SGRP

No associated diseases with P15938

6 regional properties for P15938

Type Name Position InterPro Accession
domain Helicase, C-terminal 540 - 735 IPR001650
conserved_site DNA/RNA helicase, ATP-dependent, DEAH-box type, conserved site 468 - 477 IPR002464
domain Helicase-associated domain 756 - 846 IPR007502
domain DEAD/DEAH box helicase domain 357 - 511 IPR011545
domain DEAD-box helicase, OB fold 903 - 980 IPR011709
domain Helicase superfamily 1/2, ATP-binding domain 348 - 535 IPR014001

Functions

Description
EC Number 3.6.4.13 Acting on ATP; involved in cellular and subcellular movement
Subcellular Localization
  • Nucleus
  • Binds to the spliceosome
PANTHER Family
PANTHER Subfamily
PANTHER Protein Class
PANTHER Pathway Category No pathway information available

2 GO annotations of cellular component

Name Definition
spliceosomal complex Any of a series of ribonucleoprotein complexes that contain snRNA(s) and small nuclear ribonucleoproteins (snRNPs), and are formed sequentially during the spliceosomal splicing of one or more substrate RNAs, and which also contain the RNA substrate(s) from the initial target RNAs of splicing, the splicing intermediate RNA(s), to the final RNA products. During cis-splicing, the initial target RNA is a single, contiguous RNA transcript, whether mRNA, snoRNA, etc., and the released products are a spliced RNA and an excised intron, generally as a lariat structure. During trans-splicing, there are two initial substrate RNAs, the spliced leader RNA and a pre-mRNA.
U2-type catalytic step 2 spliceosome A spliceosomal complex that contains the U2, U5 and U6 snRNPs bound to a splicing intermediate in which the first catalytic cleavage of the 5' splice site has occurred. The precise subunit composition differs significantly from that of the catalytic step 1, or activated, spliceosome, and includes many proteins in addition to those found in the U2, U5 and U6 snRNPs.

5 GO annotations of molecular function

Name Definition
ATP binding Binding to ATP, adenosine 5'-triphosphate, a universally important coenzyme and enzyme regulator.
ATP hydrolysis activity Catalysis of the reaction: ATP + H2O = ADP + H+ phosphate. ATP hydrolysis is used in some reactions as an energy source, for example to catalyze a reaction or drive transport against a concentration gradient.
ATP-dependent activity, acting on RNA Catalysis of the reaction: ATP + H2O = ADP + phosphate; this reaction requires the presence of RNA, and it drives another reaction.
RNA binding Binding to an RNA molecule or a portion thereof.
RNA helicase activity Unwinding of an RNA helix, driven by ATP hydrolysis.

5 GO annotations of biological process

Name Definition
generation of catalytic spliceosome for second transesterification step Conformational rearrangement of the spliceosomal complex containing the RNA products from the 1st step of splicing to form the catalytic site for the second step of splicing.
mRNA splicing, via spliceosome The joining together of exons from one or more primary transcripts of messenger RNA (mRNA) and the excision of intron sequences, via a spliceosomal mechanism, so that mRNA consisting only of the joined exons is produced.
RNA exon ligation The RNA metabolic process that joins two exons, each of which has free ends that were generated by endonucleolytic cleavages, by a ligation reaction.
snRNA metabolic process The chemical reactions and pathways involving snRNA, small nuclear RNA, any of various low-molecular-mass RNA molecules found in the eukaryotic nucleus as components of the small nuclear ribonucleoprotein.
snRNA modification The covalent alteration of one or more nucleotides within snRNA, resulting in a change in the properties of the snRNA.

5 homologous proteins in AiPD

UniProt AC Gene Name Protein Name Species Evidence Code
P36009 DHR2 Probable ATP-dependent RNA helicase DHR2 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) PR
P20095 PRP2 Pre-mRNA-splicing factor ATP-dependent RNA helicase-like protein PRP2 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) PR
P43329 hrpA ATP-dependent RNA helicase HrpA Escherichia coli (strain K12) PR
Q92620 DHX38 Pre-mRNA-splicing factor ATP-dependent RNA helicase PRP16 Homo sapiens (Human) PR
P34498 mog-1 Probable pre-mRNA-splicing factor ATP-dependent RNA helicase mog-1 Caenorhabditis elegans PR
10 20 30 40 50 60
MGHSGREERI KDIFKELTSK ELTPGLLLTL QKLAQKPNTN LEQFIASCKA LTKLSSNNPI
70 80 90 100 110 120
IFNELLELLK NKSEEDSTGP KKIAPSINKR KKFKIQLDLD DNEDELDSPV QKKPAPTRTL
130 140 150 160 170 180
FKRIDKLKAK QLRQYSPTVK DPSPNSEQQT QNGHAETKDY EPTRSEVVEE DREWYDNDDD
190 200 210 220 230 240
YGNLVPEPLS ELPEEAKLLP VIRNIDNDDA LRNTVQLYPI PLKQRMEWIP PFLSKFALEN
250 260 270 280 290 300
KVPTSIIIGS ISETSSQVSA LSMVNPFRNP DSEFSANAKR GSKLVALRRI NMEHIQQSRD
310 320 330 340 350 360
NTTVLNTAMG EVLGLENNNK AKDKSNQKIC DDTALFTPSK DDIKHTKEQL PVFRCRSQLL
370 380 390 400 410 420
SLIRENQVVV IIGETGSGKT TQLAQYLYEE GYANDRGKSI VVTQPRRVAA ISVAKRVAME
430 440 450 460 470 480
MQVPLGKEVG YSIRFEDVTD SECTKLKFVT DGILLRETLL DDTLDKYSCV IIDEAHERSL
490 500 510 520 530 540
NTDILLGFFK ILLARRRDLK LIITSATMNA KKFSAFFGNA PQFTIPGRTF PVQTIYTSNP
550 560 570 580 590 600
VQDYVEAAVS QAVKIHLAND CSSGDILIFM TGQEDIETTF DTLQEKFLQV YSKKFGTANF
610 620 630 640 650 660
EEINDIEILP IYSALPADLQ FKIFQDLHGT KRKIIIATNI AETSLTIKGI RYVIDCGYSK
670 680 690 700 710 720
LKVYNPKIGL DSLVITPISK ANADQRSGRA GRTAPGTAYR LYTEDTFKED MYLQTIPEIQ
730 740 750 760 770 780
RTNLSNTLLL LKSLDVTDEL SKFPFIDKPP LQTFLSSLYE LWFIGAIDTS GQLTPLGLQM
790 800 810 820 830 840
AKFPLQPSLS KILLIAVRNG CSDEMLTIVS MLSVPQVFYR PKERQKEADI ARNKFFIAKS
850 860 870 880 890 900
DHLTLLNVFE QWRANNFSSH WCNKHFVQYK SLVRARDIRD QLLTILKSQK IPVISSGKDW
910 920 930 940 950 960
DIIKKCICSG FAHQAAKITG LRNYVHLKTG VSVQLHPTSA LHGLGDLPPY VVYHELLMTS
970 980 990 1000 1010 1020
KEYICCVTSV DPFWLMEYGG LLYDIKRIKN DQEATTTGLF GEHYEHTLDK VEDDIDINIR
1030 1040 1050 1060 1070
RCKDMRDSVI QELKMTDNSN KEDKKQKTKK QNILNGKENS MKPFKRRKPF F