P20095

PR
Gene name
PRP2 (RNA2, YNR011C, N2048)
Protein name
Pre-mRNA-splicing factor ATP-dependent RNA helicase-like protein PRP2
Names
Pre-mRNA-processing protein 2
Species
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
KEGG Pathway
sce:YNR011C
EC number
3.6.4.13: Acting on ATP; involved in cellular and subcellular movement
Protein Class

Descriptions

The autoinhibited protein was predicted that may have potential autoinhibitory elements via cis-regPred.

Autoinhibitory domains (AIDs)

1-186 (Predicted disordered autoinhibitory region) PR

Target domain

Relief mechanism

Assay

cis-regPred

Accessory elements

No accessory elements

References

Autoinhibited structure

Activated structure

8 structures for P20095

Entry ID Method Resolution Chain Position Source
5GM6 EM 350 A Y 1-876 PDB
5LQW EM 580 A O 1-876 PDB
7DCO EM 250 A x 1-876 PDB
7DCP EM 315 A x 12-867 PDB
7DCQ EM 290 A x 1-876 PDB
7DCR EM 315 A x 1-876 PDB
7DD3 EM 320 A x 1-876 PDB
AF-P20095-F1 Predicted AlphaFoldDB

11 variants for P20095

Variant ID(s) Position Change Description Diseaes Association Provenance
s14-646937 6 S>P No SGRP
s14-646801 51 L>P No SGRP
s14-646757 66 N>D No SGRP
s14-646719 78 Q>H No SGRP
s14-646696 86 S>L No SGRP
s14-646549 135 I>S No SGRP
s14-646159 265 F>Y No SGRP
s14-645664 430 T>I No SGRP
s14-645371 528 V>I No SGRP
s14-644729 742 N>Y No SGRP
s14-644582 791 G>C No SGRP

No associated diseases with P20095

6 regional properties for P20095

Type Name Position InterPro Accession
domain Helicase, C-terminal 540 - 735 IPR001650
conserved_site DNA/RNA helicase, ATP-dependent, DEAH-box type, conserved site 468 - 477 IPR002464
domain Helicase-associated domain 756 - 846 IPR007502
domain DEAD/DEAH box helicase domain 357 - 511 IPR011545
domain DEAD-box helicase, OB fold 903 - 980 IPR011709
domain Helicase superfamily 1/2, ATP-binding domain 348 - 535 IPR014001

Functions

Description
EC Number 3.6.4.13 Acting on ATP; involved in cellular and subcellular movement
Subcellular Localization
  • Nucleus
PANTHER Family
PANTHER Subfamily
PANTHER Protein Class
PANTHER Pathway Category No pathway information available

1 GO annotations of cellular component

Name Definition
U2-type catalytic step 1 spliceosome A spliceosomal complex that is formed by the displacement of the U1 and U4 snRNPs from the precatalytic spliceosome; the U2, U5 and U6 snRNPs remain associated with the mRNA. This complex, sometimes called the activated spliceosome, is the catalytically active form of the spliceosome, and includes many proteins in addition to those found in the U2, and U5 and U6 snRNPs.

5 GO annotations of molecular function

Name Definition
ATP binding Binding to ATP, adenosine 5'-triphosphate, a universally important coenzyme and enzyme regulator.
ATP hydrolysis activity Catalysis of the reaction: ATP + H2O = ADP + H+ phosphate. ATP hydrolysis is used in some reactions as an energy source, for example to catalyze a reaction or drive transport against a concentration gradient.
ATP-dependent activity, acting on RNA Catalysis of the reaction: ATP + H2O = ADP + phosphate; this reaction requires the presence of RNA, and it drives another reaction.
RNA binding Binding to an RNA molecule or a portion thereof.
RNA helicase activity Unwinding of an RNA helix, driven by ATP hydrolysis.

2 GO annotations of biological process

Name Definition
generation of catalytic spliceosome for first transesterification step Formation of a catalytic spliceosome complex ready to perform the first splicing reaction. This occurs by an ATP-dependent conformational change of the pre-catalytic spliceosome.
snoRNA splicing The process of removing sections of a primary snoRNA transcript to remove sequences not present in the mature form of the snoRNA and joining the remaining sections to form the mature form of the snoRNA.

4 homologous proteins in AiPD

UniProt AC Gene Name Protein Name Species Evidence Code
P36009 DHR2 Probable ATP-dependent RNA helicase DHR2 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) PR
P15938 PRP16 Pre-mRNA-splicing factor ATP-dependent RNA helicase PRP16 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) PR
P43329 hrpA ATP-dependent RNA helicase HrpA Escherichia coli (strain K12) PR
O60231 DHX16 Pre-mRNA-splicing factor ATP-dependent RNA helicase DHX16 Homo sapiens (Human) PR
10 20 30 40 50 60
MSSITSETGK RRVKRTYEVT RQNDNAVRIE PSSLGEEEDK EAKDKNSALQ LKRSRYDPNK
70 80 90 100 110 120
VFSNTNQGPE KNNLKGEQLG SQKKSSKYDE KITSNNELTT KKGLLGDSEN ETKYASSNSK
130 140 150 160 170 180
FNVEVTHKIK NAKEIDKINR QRMWEEQQLR NAMAGQSDHP DDITLEGSDK YDYVFDTDAM
190 200 210 220 230 240
IDYTNEEDDL LPEEKLQYEA RLAQALETEE KRILTIQEAR KLLPVHQYKD ELLQEIKKNQ
250 260 270 280 290 300
VLIIMGETGS GKTTQLPQYL VEDGFTDQGK LQIAITQPRR VAATSVAARV ADEMNVVLGK
310 320 330 340 350 360
EVGYQIRFED KTTPNKTVLK YMTDGMLLRE FLTDSKLSKY SCIMIDEAHE RTLATDILIG
370 380 390 400 410 420
LLKDILPQRP TLKLLISSAT MNAKKFSEFF DNCPIFNVPG RRYPVDIHYT LQPEANYIHA
430 440 450 460 470 480
AITTIFQIHT TQSLPGDILV FLTGQEEIER TKTKLEEIMS KLGSRTKQMI ITPIYANLPQ
490 500 510 520 530 540
EQQLKIFQPT PENCRKVVLA TNIAETSLTI DGIRYVIDPG FVKENSYVPS TGMTQLLTVP
550 560 570 580 590 600
CSRASVDQRA GRAGRVGPGK CFRIFTKWSY LHELELMPKP EITRTNLSNT VLLLLSLGVT
610 620 630 640 650 660
DLIKFPLMDK PSIPTLRKSL ENLYILGALN SKGTITRLGK MMCEFPCEPE FAKVLYTAAT
670 680 690 700 710 720
HEQCQGVLEE CLTIVSMLHE TPSLFIGQKR DAAASVLSEV ESDHILYLEI FNQWRNSKFS
730 740 750 760 770 780
RSWCQDHKIQ FKTMLRVRNI RNQLFRCSEK VGLVEKNDQA RMKIGNIAGY INARITRCFI
790 800 810 820 830 840
SGFPMNIVQL GPTGYQTMGR SSGGLNVSVH PTSILFVNHK EKAQRPSKYV LYQQLMLTSK
850 860 870
EFIRDCLVIP KEEWLIDMVP QIFKDLIDDK TNRGRR