AiPD: Autoinhibited Protein Database

Gene name	Uba2 (Sae2, Uble1b)
Protein name	SUMO-activating enzyme subunit 2
Names	Anthracycline-associated resistance ARX, Ubiquitin-like 1-activating enzyme E1B, Ubiquitin-like modifier-activating enzyme 2
Species	Mus musculus (Mouse)
KEGG Pathway	mmu:50995
EC number
Protein Class

The autoinhibited protein was predicted that may have potential autoinhibitory elements via cis-regPred.

174-333 (Predicted disordered autoinhibitory region) PR

174-333 (Predicted disordered autoinhibitory region)

cis-regPred

No accessory elements

Entry ID	Method	Resolution	Chain	Position	Source
AF-Q9Z1F9-F1	Predicted				AlphaFoldDB

Variant ID(s)	Position	Change	Diseaes Association	Provenance
rs3388903733	65	K>R	No	EVA
rs3397814172	67	H>Q	No	EVA
rs3388891426	113	M>I	No	EVA
rs3388897289	119	R>I	No	EVA
rs3388873235	141	T>S	No	EVA
rs3388903048	158	C>Y	No	EVA
rs3388873290	174	T>M	No	EVA
rs3388903738	197	F>*	No	EVA
rs3388888043	214	E>*	No	EVA
rs223332811	220	T>A	No	EVA
rs3388901337	220	T>K	No	EVA
rs3397911847	257	K>*	No	EVA
rs3388873247	259	F>L	No	EVA
rs3388897749	264	R>I	No	EVA
rs3388900407	370	S>C	No	EVA
rs3388859933	448	V>M	No	EVA
rs3388904651	480	I>F	No	EVA
rs3388893257	516	Q>R	No	EVA
rs3388897711	519	D>V	No	EVA
rs3388903763	563	S>C	No	EVA

Type	Name	Position	InterPro Accession
domain	THIF-type NAD/FAD binding fold	9 - 440	IPR000594
conserved_site	Ubiquitin-activating enzyme E1, conserved site	402 - 410	IPR018074
domain	Ubiquitin/SUMO-activating enzyme ubiquitin-like domain	450 - 535	IPR028077
domain	SUMO-activating enzyme subunit 2, C-terminal domain	547 - 628	IPR032426
active_site	Ubiquitin-activating enzyme E1, Cys active site	171 - 179	IPR033127

		Description
EC Number
Subcellular Localization	Cytoplasm Nucleus	Shuttles between the cytoplasm and the nucleus, sumoylation is required either for nuclear translocation or nuclear retention
PANTHER Family
PANTHER Subfamily
PANTHER Protein Class
PANTHER Pathway Category	No pathway information available

Name	Definition
cytoplasm	The contents of a cell excluding the plasma membrane and nucleus, but including other subcellular structures.
nucleoplasm	That part of the nuclear content other than the chromosomes or the nucleolus.
SUMO activating enzyme complex	A conserved heterodimeric complex with SUMO activating enzyme activity.

Name	Definition
ATP binding	Binding to ATP, adenosine 5'-triphosphate, a universally important coenzyme and enzyme regulator.
magnesium ion binding	Binding to a magnesium (Mg) ion.
protein heterodimerization activity	Binding to a nonidentical protein to form a heterodimer.
small protein activating enzyme binding	Binding to a small protein activating enzyme, such as ubiquitin-activating enzyme.
SUMO activating enzyme activity	Catalysis of the activation of the proteolytically processed small ubiquitin-related modifier SUMO, through the formation of an ATP-dependent high-energy thiolester bond.
SUMO binding	Binding to the small ubiquitin-like protein SUMO.
transferase activity	Catalysis of the transfer of a group, e.g. a methyl group, glycosyl group, acyl group, phosphorus-containing, or other groups, from one compound (generally regarded as the donor) to another compound (generally regarded as the acceptor). Transferase is the systematic name for any enzyme of EC class 2.
ubiquitin-like protein conjugating enzyme binding	Binding to a ubiquitin-like protein conjugating enzyme such as ubiquitin conjugating enzyme.

Name	Definition
positive regulation of protein sumoylation	Any process that activates or increases the frequency, rate or extent of the addition of SUMO groups to a protein.
protein modification by small protein conjugation	A protein modification process in which one or more groups of a small protein, such as ubiquitin or a ubiquitin-like protein, are covalently attached to a target protein.
protein sumoylation	The process in which a SUMO protein (small ubiquitin-related modifier) is conjugated to a target protein via an isopeptide bond between the carboxy-terminus of SUMO with an epsilon-amino group of a lysine residue of the target protein.

UniProt AC	Gene Name	Protein Name	Species	Evidence Code
Q9UBT2	UBA2	SUMO-activating enzyme subunit 2	Homo sapiens (Human)	PR
Q02053	Uba1	Ubiquitin-like modifier-activating enzyme 1	Mus musculus (Mouse)	PR
Q8VE47	Uba5	Ubiquitin-like modifier-activating enzyme 5	Mus musculus (Mouse)	PR
P31254	Uba1y	Ubiquitin-like modifier-activating enzyme 1 Y	Mus musculus (Mouse)	PR
Q8C878	Uba3	NEDD8-activating enzyme E1 catalytic subunit	Mus musculus (Mouse)	PR
Q7SXG4	uba2	SUMO-activating enzyme subunit 2	Danio rerio (Zebrafish) (Brachydanio rerio)	PR