Q05763

PR
Gene name
THY-2 (At4g34570, T4L20.150)
Protein name
Bifunctional dihydrofolate reductase-thymidylate synthase 2
Names
DHFR-TS 2
Species
Arabidopsis thaliana (Mouse-ear cress)
KEGG Pathway
ath:AT4G34570
EC number
1.5.1.3: With NAD(+) or NADP(+) as acceptor
Protein Class

Descriptions

The autoinhibited protein was predicted that may have potential autoinhibitory elements via cis-regPred.

Autoinhibitory domains (AIDs)

1-48 (Predicted disordered autoinhibitory region) PR

Target domain

Relief mechanism

Assay

cis-regPred

Accessory elements

No accessory elements

References

Autoinhibited structure

Activated structure

1 structures for Q05763

Entry ID Method Resolution Chain Position Source
AF-Q05763-F1 Predicted AlphaFoldDB

46 variants for Q05763

Variant ID(s) Position Change Description Diseaes Association Provenance
ENSVATH06824030 14 A>E No 1000Genomes
tmp_4_16513530_C_A 26 W>L No 1000Genomes
tmp_4_16513497_A_G 37 L>S No 1000Genomes
tmp_4_16513438_T_A 57 I>F No 1000Genomes
ENSVATH06824025 81 D>N No 1000Genomes
tmp_4_16513353_G_A 85 P>L No 1000Genomes
ENSVATH00552508 90 T>I No 1000Genomes
tmp_4_16513339_T_A 90 T>S No 1000Genomes
ENSVATH06824023 97 D>G No 1000Genomes
ENSVATH12390659 128 L>F No 1000Genomes
ENSVATH00552506 137 S>T No 1000Genomes
tmp_4_16513187_G_C 140 S>R No 1000Genomes
ENSVATH06824021 141 G>D No 1000Genomes
tmp_4_16513174_C_T 145 A>T No 1000Genomes
tmp_4_16513159_C_T 150 V>I No 1000Genomes
tmp_4_16512923_C_G 202 D>H No 1000Genomes
tmp_4_16512911_C_T 206 D>N No 1000Genomes
ENSVATH02957485 218 A>G No 1000Genomes
ENSVATH00552505 221 P>S No 1000Genomes
ENSVATH06824018 245 S>R No 1000Genomes
ENSVATH06824017 246 S>T No 1000Genomes
ENSVATH12390656 253 D>A No 1000Genomes
tmp_4_16512761_C_A 256 D>Y No 1000Genomes
ENSVATH06824016 277 F>S No 1000Genomes
tmp_4_16512638_T_G 297 N>H No 1000Genomes
ENSVATH02957484 299 K>T No 1000Genomes
tmp_4_16512628_T_G 300 D>A No 1000Genomes
ENSVATH06824015 314 Q>H No 1000Genomes
ENSVATH12390594 346 G>C No 1000Genomes
ENSVATH12390592 365 A>V No 1000Genomes
ENSVATH14336559 368 A>V No 1000Genomes
ENSVATH06824010 379 E>G No 1000Genomes
tmp_4_16511983_T_A 380 E>D No 1000Genomes
ENSVATH02957478 400 D>N No 1000Genomes
tmp_4_16511794_A_G 413 L>P No 1000Genomes
ENSVATH06824008 432 S>L No 1000Genomes
ENSVATH02957477 445 P>H No 1000Genomes
tmp_4_16511685_C_T 449 M>I No 1000Genomes
tmp_4_16511569_A_G 488 I>T No 1000Genomes
ENSVATH06824007 491 H>Q No 1000Genomes
ENSVATH02957475 496 V>A No 1000Genomes
ENSVATH06824003 511 K>I No 1000Genomes
ENSVATH06824003 511 K>T No 1000Genomes
ENSVATH06823999 539 D>H No 1000Genomes
ENSVATH06823996 560 D>N No 1000Genomes
tmp_4_16511133_A_C 565 V>G No 1000Genomes

No associated diseases with Q05763

4 regional properties for Q05763

Type Name Position InterPro Accession
domain Dihydrofolate reductase domain 65 - 242 IPR001796
conserved_site Dihydrofolate reductase conserved site 77 - 99 IPR017925
active_site Thymidylate synthase, active site 427 - 455 IPR020940
domain Thymidylate synthase/dCMP hydroxymethylase domain 285 - 565 IPR023451

Functions

Description
EC Number 1.5.1.3 With NAD(+) or NADP(+) as acceptor
Subcellular Localization
PANTHER Family
PANTHER Subfamily
PANTHER Protein Class
PANTHER Pathway Category No pathway information available

2 GO annotations of cellular component

Name Definition
cytosol The part of the cytoplasm that does not contain organelles but which does contain other particulate matter, such as protein complexes.
mitochondrion A semiautonomous, self replicating organelle that occurs in varying numbers, shapes, and sizes in the cytoplasm of virtually all eukaryotic cells. It is notably the site of tissue respiration.

2 GO annotations of molecular function

Name Definition
dihydrofolate reductase activity Catalysis of the reaction: 5,6,7,8-tetrahydrofolate + NADP+ = 7,8-dihydrofolate + NADPH + H+.
thymidylate synthase activity Catalysis of the reaction: 5,10-methylenetetrahydrofolate + dUMP = 7,8-dihydrofolate + thymidylate.

4 GO annotations of biological process

Name Definition
10-formyltetrahydrofolate biosynthetic process The chemical reactions and pathways resulting in the formation of 10-formyltetrahydrofolate, the formylated derivative of tetrahydrofolate.
dTMP biosynthetic process The chemical reactions and pathways resulting in the formation of dTMP, deoxyribosylthymine monophosphate (2'-deoxyribosylthymine 5'-phosphate).
methylation The process in which a methyl group is covalently attached to a molecule.
one-carbon metabolic process The chemical reactions and pathways involving the transfer of one-carbon units in various oxidation states.

5 homologous proteins in AiPD

UniProt AC Gene Name Protein Name Species Evidence Code
O76511 Ts Thymidylate synthase Drosophila melanogaster (Fruit fly) PR
P04818 TYMS Thymidylate synthase Homo sapiens (Human) PR
O81395 DRTS Bifunctional dihydrofolate reductase-thymidylate synthase Zea mays (Maize) PR
P07607 Tyms Thymidylate synthase Mus musculus (Mouse) PR
P45352 Tyms Thymidylate synthase Rattus norvegicus (Rat) PR
10 20 30 40 50 60
MRCLQNSAKT LPLAFKSALL PLSQRWFCKF SPKPSSLTNI FKVSISTMAN TLNGNVIMTS
70 80 90 100 110 120
KPQSTYQVVV AATKEMGIGK DGKLPWNLPT DLKFFKDLTL STSDSAKKNA VVMGRKTWES
130 140 150 160 170 180
IPKKYRPLSG RLNVVLSRSS GFDIANTENV VTCSSIDSAL DLLAAPPFSL SIEKVFVIGG
190 200 210 220 230 240
GDILREALNK PSCEAIHITE IDTSIDCDTF IPTVDTSAYQ PWCSSFPICE NGLRFSFTTH
250 260 270 280 290 300
VRVKSSSAGE ASDESDGSKV LQVDWKKFSS VLPKMIFDRH EEYLYLNLVK EIISNGNLKD
310 320 330 340 350 360
DRTGTGTLSK FGCQMKFNLR RNFPLLTTKR VFWRGVVEEL LWFISGSTNA KVLQEKGIRI
370 380 390 400 410 420
WDGNASRAYL DGIGLTEREE GDLGPVYGFQ WRHFGAKYTD MHADYTGQGF DQLLDVINKI
430 440 450 460 470 480
KNNPDDRRII MSAWNPSDLK LMALPPCHMF AQFYVANGEL SCQMYQRSAD MGLGVPFNIA
490 500 510 520 530 540
SYSLLTCILA HVCDLVPGDF IHVIGDAHVY KNHVRPLQEQ LENPPKPFPV LKINPEKKDI
550 560
DSFVADDFEL IGYDPHKKID MKMAV