O81395

PR
Gene name
DRTS
Protein name
Bifunctional dihydrofolate reductase-thymidylate synthase
Names
DHFR-TS
Species
Zea mays (Maize)
KEGG Pathway
zma:541707
EC number
1.5.1.3: With NAD(+) or NADP(+) as acceptor
Protein Class

Descriptions

The autoinhibited protein was predicted that may have potential autoinhibitory elements via cis-regPred.

Autoinhibitory domains (AIDs)

1-42 (Predicted disordered autoinhibitory region) PR

Target domain

Relief mechanism

Assay

cis-regPred

Accessory elements

No accessory elements

References

Autoinhibited structure

Activated structure

1 structures for O81395

Entry ID Method Resolution Chain Position Source
AF-O81395-F1 Predicted AlphaFoldDB

No variants for O81395

Variant ID(s) Position Change Description Diseaes Association Provenance
No variants for O81395

No associated diseases with O81395

4 regional properties for O81395

Type Name Position InterPro Accession
domain Dihydrofolate reductase domain 17 - 194 IPR001796
conserved_site Dihydrofolate reductase conserved site 29 - 51 IPR017925
active_site Thymidylate synthase, active site 383 - 411 IPR020940
domain Thymidylate synthase/dCMP hydroxymethylase domain 240 - 521 IPR023451

Functions

Description
EC Number 1.5.1.3 With NAD(+) or NADP(+) as acceptor
Subcellular Localization
PANTHER Family
PANTHER Subfamily
PANTHER Protein Class
PANTHER Pathway Category No pathway information available

2 GO annotations of cellular component

Name Definition
cytosol The part of the cytoplasm that does not contain organelles but which does contain other particulate matter, such as protein complexes.
mitochondrion A semiautonomous, self replicating organelle that occurs in varying numbers, shapes, and sizes in the cytoplasm of virtually all eukaryotic cells. It is notably the site of tissue respiration.

2 GO annotations of molecular function

Name Definition
dihydrofolate reductase activity Catalysis of the reaction: 5,6,7,8-tetrahydrofolate + NADP+ = 7,8-dihydrofolate + NADPH + H+.
thymidylate synthase activity Catalysis of the reaction: 5,10-methylenetetrahydrofolate + dUMP = 7,8-dihydrofolate + thymidylate.

4 GO annotations of biological process

Name Definition
dTMP biosynthetic process The chemical reactions and pathways resulting in the formation of dTMP, deoxyribosylthymine monophosphate (2'-deoxyribosylthymine 5'-phosphate).
methylation The process in which a methyl group is covalently attached to a molecule.
one-carbon metabolic process The chemical reactions and pathways involving the transfer of one-carbon units in various oxidation states.
tetrahydrofolate biosynthetic process The chemical reactions and pathways resulting in the formation of tetrahydrofolate, 5,6,7,8-tetrahydrofolic acid, a folate derivative bearing additional hydrogens on the pterin group.

5 homologous proteins in AiPD

UniProt AC Gene Name Protein Name Species Evidence Code
O76511 Ts Thymidylate synthase Drosophila melanogaster (Fruit fly) PR
P04818 TYMS Thymidylate synthase Homo sapiens (Human) PR
P07607 Tyms Thymidylate synthase Mus musculus (Mouse) PR
P45352 Tyms Thymidylate synthase Rattus norvegicus (Rat) PR
Q05763 THY-2 Bifunctional dihydrofolate reductase-thymidylate synthase 2 Arabidopsis thaliana (Mouse-ear cress) PR
10 20 30 40 50 60
MAAVLANGDS QGRPQRNYQV VVAGTRDMGI GKDGVLPWKL PGDLKFFKEL TLTTSDPVKK
70 80 90 100 110 120
NAVIMGRKTW ESIPVKSRPL PGRLNVILTR SGSFDFATVE NVVICGSMES ALELLASTPY
130 140 150 160 170 180
CLSIEKVFVI GGGQVLREYL KGPACEAIHL TDIQSSIECD TFIPPVDFSV FQPWYSSFPV
190 200 210 220 230 240
IESNIRHSFV SFVRVRKSVA ETHESNGKES TEVDTKNDKF ETENFSFLPK MVYDRHEEYQ
250 260 270 280 290 300
YLNLVEDIIR SGAQKNDRTG TGTLSKFGCQ MRFNLRKNFP LLTTKRVFWR GVVEELLWFI
310 320 330 340 350 360
SGSTNAKVLQ EKGIHIWDGN ASREYLNSVG LAHREEGDLG PIYGFQWRHF GAEYTDMHAD
370 380 390 400 410 420
YTGKGFDQLM DVIDKIKNDP EDRRIILSAW NPSDLKKMAL PPCHMFAQFY VENGELSCQM
430 440 450 460 470 480
YQRSADMGLG VPFNIASYSL LTYMIAQVCD LSPGDFVHVI GDAHVYRNHV RALEEQIQKM
490 500 510 520
PKPFPILKIN PSKKDIDSFM ASDFKLVGYD PHQKIEMKMA V