AiPD: Autoinhibited Protein Database

P04818

Gene name	TYMS
Protein name	Thymidylate synthase
Names	TS, TSase
Species	Homo sapiens (Human)
KEGG Pathway	hsa:7298
EC number	2.1.1.45: Methyltransferases
Protein Class

Descriptions

The autoinhibited protein was predicted that may have potential autoinhibitory elements via cis-regPred.

Autoinhibitory domains (AIDs)

1-57 (Predicted disordered autoinhibitory region) PR

Target domain
Relief mechanism
Assay	cis-regPred

AID

1-57 (Predicted disordered autoinhibitory region)

Target domain

Relief mechanism

Assay

cis-regPred

Accessory elements

No accessory elements

References

Yeon JH et al. (2016) "Systems-wide Identification of cis-Regulatory Elements in Proteins", Cell Systems, 2, 89-100

Autoinhibited structure

Activated structure

62 structures for P04818

Entry ID	Method	Resolution	Chain	Position	Source
1HVY	X-ray	190 A	A/B/C/D	26-313	PDB
1HW3	X-ray	200 A	A	1-313	PDB
1HW4	X-ray	206 A	A	1-313	PDB
1HZW	X-ray	200 A	A/B	1-313	PDB
1I00	X-ray	250 A	A/B	1-313	PDB
1JU6	X-ray	289 A	A/B/C/D	1-313	PDB
1JUJ	X-ray	300 A	A/B/C/D	1-313	PDB
1YPV	X-ray	180 A	A	1-313	PDB
2ONB	X-ray	270 A	A	1-313	PDB
2RD8	X-ray	250 A	A/B	1-313	PDB
2RDA	X-ray	267 A	A/B/C/D/E/F	1-313	PDB
3EAW	X-ray	186 A	X	1-313	PDB
3EBU	X-ray	205 A	A	1-313	PDB
3ED7	X-ray	156 A	A	26-313	PDB
3EDW	X-ray	175 A	X	1-313	PDB
3EF9	X-ray	320 A	A	1-313	PDB
3EGY	X-ray	218 A	X	1-313	PDB
3EHI	X-ray	200 A	X	1-313	PDB
3EJL	X-ray	320 A	A/B/C/D	1-313	PDB
3GG5	X-ray	277 A	A/B/C/D	1-313	PDB
3GH0	X-ray	156 A	A	1-313	PDB
3GH2	X-ray	175 A	X	1-313	PDB
3H9K	X-ray	265 A	A/B/C/D/E	1-313	PDB
3HB8	X-ray	274 A	A/B/C/D/E	1-313	PDB
3N5E	X-ray	226 A	A/B	1-313	PDB
3N5G	X-ray	227 A	A	1-313	PDB
3OB7	X-ray	275 A	A/B/C/D/E	1-313	PDB
4E28	X-ray	230 A	A	1-313	PDB
4FGT	X-ray	200 A	A	1-311	PDB
4G2O	X-ray	225 A	X	1-313	PDB
4G6W	X-ray	230 A	X	1-313	PDB
4GD7	X-ray	229 A	A	1-313	PDB
4GYH	X-ray	300 A	A	1-313	PDB
4H1I	X-ray	310 A	A/B/C/D	1-313	PDB
4JEF	X-ray	231 A	A	26-311	PDB
4KPW	X-ray	203 A	A	1-313	PDB
4O1U	X-ray	226 A	A/B	1-313	PDB
4O1X	X-ray	232 A	A/B/C/D	1-313	PDB
4UP1	X-ray	299 A	A/B/C/D	1-313	PDB
5HS3	X-ray	310 A	A/B/C/D/E/F	26-313	PDB
5WRN	X-ray	239 A	A/B/C/D/E/F	26-313	PDB
5X4W	X-ray	210 A	A	1-313	PDB
5X4X	X-ray	231 A	A	1-313	PDB
5X4Y	X-ray	220 A	A	1-313	PDB
5X5A	X-ray	239 A	A/B/C/D/E/F	26-313	PDB
5X5D	X-ray	200 A	A/B/C/D/E/F	26-313	PDB
5X5Q	X-ray	279 A	A/B/C/D/E/F	26-313	PDB
5X66	X-ray	199 A	A/B/C/D/E/F	26-313	PDB
5X67	X-ray	213 A	A/B	26-313	PDB
5X69	X-ray	269 A	A/B/C/D/E/F	26-313	PDB
6OJU	X-ray	288 A	A/B/C/D	26-313	PDB
6OJV	X-ray	259 A	A/B/C/D	26-313	PDB
6PF3	X-ray	239 A	A/B/C/D	26-313	PDB
6PF4	X-ray	285 A	A/B/C/D	26-313	PDB
6PF5	X-ray	239 A	A/B/C/D	26-313	PDB
6PF6	X-ray	250 A	A/B/C/D	26-313	PDB
6QXG	X-ray	208 A	A/B/C	1-313	PDB
6QXH	X-ray	204 A	A/B/C	1-313	PDB
6QYQ	X-ray	225 A	A/B/C/D	1-313	PDB
6R2E	X-ray	255 A	A/B/C/D/E/F/G/H	1-313	PDB
6ZXO	X-ray	260 A	A/B/C/D/E/F	1-313	PDB
AF-P04818-F1	Predicted				AlphaFoldDB

193 variants for P04818

Variant ID(s)	Position	Change	Description	Diseaes Association	Provenance
VAR_087693	87	E>K	DKCD; unknown pathological significance [UniProt]	Yes	UniProt
VAR_087694	115	R>del	DKCD; reduced TYMS levels in patient cells [UniProt]	Yes	UniProt
VAR_087695	160	Q>H	DKCD; unknown pathological significance [UniProt]	Yes	UniProt
VAR_087696	271	R>del	DKCD [UniProt]	Yes	UniProt
rs1276365455 CA401668642	2	P>H		No	ClinGen gnomAD
CA295416132 rs940303227	4	A>G		No	ClinGen Ensembl
rs910134246 CA295416117	4	A>S		No	ClinGen TOPMed gnomAD
rs765119393 CA8867698	5	G>S		No	ClinGen ExAC TOPMed gnomAD
rs1337198820 CA401668661	6	S>A		No	ClinGen TOPMed gnomAD
CA401668665 rs1257230944	6	S>L		No	ClinGen TOPMed gnomAD
CA401668664 rs1257230944	6	S>W		No	ClinGen TOPMed gnomAD
rs1190217127 CA401668682	9	P>L		No	ClinGen gnomAD
CA401668687 rs1350884240	10	R>C		No	ClinGen Ensembl
rs985633324 CA295416167	10	R>H		No	ClinGen TOPMed gnomAD
rs985633324 CA401668689	10	R>L		No	ClinGen TOPMed gnomAD
CA401668696 rs1475132959	12	P>A		No	ClinGen gnomAD
CA8867700 rs758379914	13	L>S		No	ClinGen ExAC gnomAD
CA401668710 rs1172829435	14	P>H		No	ClinGen TOPMed gnomAD
CA401668712 rs1172829435	14	P>L		No	ClinGen TOPMed gnomAD
rs1172829435 CA401668711	14	P>R		No	ClinGen TOPMed gnomAD
CA295416180 rs188268314	15	P>A		No	ClinGen 1000Genomes ESP ExAC TOPMed gnomAD
CA8867702 rs746824953	15	P>H		No	ClinGen ExAC TOPMed gnomAD
CA401668714 rs746824953	15	P>R		No	ClinGen ExAC TOPMed gnomAD
CA8867701 rs188268314	15	P>S		No	ClinGen 1000Genomes ESP ExAC TOPMed gnomAD
CA401668735 rs1432304752	18	Q>H		No	ClinGen gnomAD
CA401668732 rs1244183309	18	Q>R		No	ClinGen TOPMed gnomAD
CA295416208 rs891810079	19	E>K		No	ClinGen Ensembl
CA295416209 rs914193673	22	A>T		No	ClinGen TOPMed
rs1365538416 CA401668771	24	P>R		No	ClinGen gnomAD
CA8867705 rs781495898	24	P>S		No	ClinGen ExAC TOPMed gnomAD
rs781495898 CA8867704	24	P>T		No	ClinGen ExAC TOPMed gnomAD
CA295416221 rs971608783	25	R>H		No	ClinGen gnomAD
rs971608783 CA401668777	25	R>L		No	ClinGen gnomAD
CA401668780 rs1280151022	26	P>S		No	ClinGen TOPMed gnomAD
rs945359619 CA295416224	27	P>L		No	ClinGen TOPMed gnomAD
CA401668785 rs1343318609	27	P>S		No	ClinGen gnomAD
rs556984495 CA8867706	28	H>Q		No	ClinGen 1000Genomes ExAC TOPMed gnomAD
CA401668796 rs1275814898	29	G>R		No	ClinGen TOPMed
CA295416246 rs1034044510	32	Q>E		No	ClinGen TOPMed gnomAD
CA401668840 rs1186795888	35	G>E		No	ClinGen TOPMed gnomAD
CA295416254 rs764376630 CA295416255	36	Q>H		No	ClinGen TOPMed
CA8867707 rs780327998	42	R>C		No	ClinGen ExAC gnomAD
COSM1242972 rs749802693 CA8867708	42	R>L	oesophagus [Cosmic]	No	ClinGen cosmic curated ExAC
rs1376079761 CA401668958	46	R>G		No	ClinGen gnomAD
rs1339068086 CA401668963	46	R>K		No	ClinGen TOPMed
CA401668998 rs1470616694	48	D>N		No	ClinGen TOPMed
TCGA novel	49	D>N	Variant assessed as Somatic; impact. [NCI-TCGA]	No	NCI-TCGA
rs774517504 CA8867710	50	R>L		No	ClinGen ExAC TOPMed gnomAD
CA8867711 rs762256027	52	G>S		No	ClinGen ExAC TOPMed gnomAD
CA401669075 rs1373657786	53	T>A		No	ClinGen TOPMed
rs1388727424 CA401669093	54	G>S		No	ClinGen gnomAD
rs1422282745 CA401669129	56	L>P		No	ClinGen TOPMed
CA401669144 rs1416155914	57	S>W		No	ClinGen gnomAD
rs1332414425 CA401669215	61	M>I		No	ClinGen gnomAD
CA295416277 rs1013467217	61	M>L		No	ClinGen TOPMed
rs1235927523 CA401669230	62	Q>R		No	ClinGen gnomAD
CA8867714 rs569127419	63	A>T		No	ClinGen 1000Genomes ExAC TOPMed gnomAD
rs1350646379 CA401669249	63	A>V		No	ClinGen gnomAD
rs1270163632 CA401669261	64	R>H		No	ClinGen TOPMed gnomAD
CA401669265 rs1270163632	64	R>L		No	ClinGen TOPMed gnomAD
rs1598460899 CA401669278	65	Y>F		No	ClinGen Ensembl
CA8867716 rs752269816	66	S>R		No	ClinGen ExAC gnomAD
rs1386139292 CA401669585	71	F>L		No	ClinGen gnomAD
rs998190755 CA295418116	72	P>L		No	ClinGen TOPMed
rs139713318 CA8867797	78	R>C		No	ClinGen ESP ExAC TOPMed gnomAD
CA401669623 rs1338576592	78	R>H	Variant assessed as Somatic; 0.0 impact. [NCI-TCGA]	No	ClinGen NCI-TCGA TOPMed gnomAD
CA401669655 rs1449621034	82	K>N		No	ClinGen gnomAD
CA295418174 rs954740566	82	K>T		No	ClinGen Ensembl
rs1182954109 CA401669686	87	E>A		No	ClinGen TOPMed
CA401669690 rs1459906556	87	E>D		No	ClinGen TOPMed
CA401669703 rs1258120815	90	W>R		No	ClinGen TOPMed
rs1200567277 CA401669715	91	F>Y		No	ClinGen TOPMed
CA401669859 rs1432195599	95	S>C		No	ClinGen gnomAD
rs59755869 CA8867817	100	E>Q		No	ClinGen ESP ExAC TOPMed gnomAD
rs761906575 CA8867820	106	V>M		No	ClinGen ExAC gnomAD
rs1198531715 CA401670139	111	A>S		No	ClinGen TOPMed
CA401670191 rs1255400378	116	D>G		No	ClinGen TOPMed
rs11540152 CA295420473	117	F>L		No	ClinGen Ensembl
rs1464510968 CA401670220	120	S>N		No	ClinGen gnomAD
CA401670230 rs1250166783	122	G>R		No	ClinGen gnomAD
TCGA novel	123	F>L	Variant assessed as Somatic; impact. [NCI-TCGA]	No	NCI-TCGA
rs1598464504 CA401670253	125	T>N		No	ClinGen Ensembl
rs1197981170 CA401670258	126	R>K		No	ClinGen gnomAD
rs1057376810 CA295420519	128	E>G		No	ClinGen TOPMed
rs1386677048 CA401670282	129	G>A		No	ClinGen TOPMed gnomAD
CA401670306 rs1598464536	133	P>A		No	ClinGen Ensembl
CA401670310 rs1567987421	133	P>L		No	ClinGen Ensembl
CA8867827 rs201415512	134	V>D		No	ClinGen 1000Genomes ExAC gnomAD
CA8867828 rs751072286	136	G>S		No	ClinGen ExAC gnomAD
CA401670362 rs550446568	141	H>D		No	ClinGen 1000Genomes ExAC TOPMed gnomAD
CA8867829 rs550446568	141	H>Y		No	ClinGen 1000Genomes ExAC TOPMed gnomAD
rs1323389786 CA401670375	142	F>L		No	ClinGen gnomAD
CA401670379 rs1329714595	143	G>E		No	ClinGen gnomAD
rs780803284 CA8867830	146	Y>*		No	ClinGen ExAC TOPMed gnomAD
rs200442141 CA8867831	147	R>K		No	ClinGen 1000Genomes ExAC gnomAD
rs1329031476 CA401670412	148	D>A		No	ClinGen gnomAD
CA8867832 rs756138720	148	D>E		No	ClinGen ExAC TOPMed gnomAD
rs1329031476 CA401670413	148	D>G		No	ClinGen gnomAD
CA295420617 rs940952547	149	M>I		No	ClinGen TOPMed gnomAD
rs1351759366 CA401670420	149	M>K		No	ClinGen gnomAD
CA8867833 rs779644557 COSM1523497	149	M>V	lung [Cosmic]	No	ClinGen cosmic curated ExAC gnomAD
rs376793375 CA8867836	150	E>D		No	ClinGen ESP ExAC TOPMed gnomAD
CA8867834 rs749119392	150	E>K		No	ClinGen ExAC gnomAD
CA8867835 rs749119392	150	E>Q		No	ClinGen ExAC gnomAD
rs1025008996 CA295420658	151	S>A		No	ClinGen gnomAD
rs1255807993 CA401670463	154	S>A		No	ClinGen TOPMed
rs596909 CA295425221	157	G>V		No	ClinGen Ensembl
CA8867859 rs773099801	162	Q>R		No	ClinGen ExAC gnomAD
CA401670522 rs1300447329	163	R>G		No	ClinGen gnomAD
TCGA novel	163	R>S	Variant assessed as Somatic; impact. [NCI-TCGA]	No	NCI-TCGA
rs747127849 CA8867860	164	V>A		No	ClinGen ExAC gnomAD
rs771296240 CA8867861 COSM1726448	165	I>T	liver [Cosmic]	No	ClinGen cosmic curated ExAC gnomAD
rs777163457 CA8867862	166	D>Y		No	ClinGen ExAC gnomAD
rs11540153 CA295425236	167	T>I		No	ClinGen Ensembl
CA401670550 rs11540153	167	T>N		No	ClinGen Ensembl
CA401670560 rs1385336277	169	K>E		No	ClinGen TOPMed gnomAD
CA8867863 rs147052596	170	T>S		No	ClinGen ESP ExAC TOPMed gnomAD
CA401670579 rs1296735999	171	N>K		No	ClinGen TOPMed
CA401670592 rs770269195	173	D>E		No	ClinGen ExAC TOPMed gnomAD
CA8867865 rs776063973	174	D>N		No	ClinGen ExAC gnomAD
rs761487013 CA8867866	176	R>I		No	ClinGen ExAC TOPMed gnomAD
rs767209304 CA8867867	176	R>S		No	ClinGen ExAC TOPMed gnomAD
CA401670611 rs761487013	176	R>T		No	ClinGen ExAC TOPMed gnomAD
CA401670618 rs1453521294	177	I>T		No	ClinGen TOPMed
rs1244429666 CA401670644	181	A>T		No	ClinGen TOPMed gnomAD
rs760265884 CA8867869	183	N>D		No	ClinGen ExAC gnomAD
rs753757524 CA8867871	185	R>K		No	ClinGen ExAC TOPMed gnomAD
TCGA novel	187	L>V	Variant assessed as Somatic; impact. [NCI-TCGA]	No	NCI-TCGA
CA401658342 rs1442205470	190	M>T		No	ClinGen TOPMed
CA8867892 rs376500183 COSM1611375	191	A>V	liver [Cosmic]	No	ClinGen cosmic curated ESP ExAC TOPMed gnomAD
TCGA novel	192	L>M	Variant assessed as Somatic; impact. [NCI-TCGA]	No	NCI-TCGA
rs1324146404 CA401658432	195	C>S		No	ClinGen gnomAD
rs746052476 CA295393307	202	Y>C		No	ClinGen Ensembl
rs1019504442 CA295393318	211	Q>H		No	ClinGen TOPMed
CA8867896 rs373096647	211	Q>L		No	ClinGen ESP ExAC TOPMed gnomAD
rs201232749 CA295393338	216	S>L		No	ClinGen gnomAD
rs201232749 CA401658851	216	S>W		No	ClinGen gnomAD
rs1598479109 CA401658909	219	M>I		No	ClinGen Ensembl
rs764943124 CA295393346	221	L>F		No	ClinGen gnomAD
CA401658961 rs1396826934	222	G>S		No	ClinGen TOPMed
rs745957549 CA8867901	227	I>M		No	ClinGen ExAC gnomAD
rs1162827398 CA401659346	227	I>V		No	ClinGen TOPMed
CA401659360 rs559346114	228	A>S		No	ClinGen 1000Genomes ExAC TOPMed gnomAD
CA8867902 rs559346114	228	A>T		No	ClinGen 1000Genomes ExAC TOPMed gnomAD
CA401659367 rs1369690699	229	S>C		No	ClinGen TOPMed
rs772325743 CA295393355	229	S>N		No	ClinGen TOPMed
CA401659372 rs772325743	229	S>T		No	ClinGen TOPMed
rs1304708715 CA401659397	231	A>T		No	ClinGen gnomAD
CA401659439 rs1374898824	235	Y>F		No	ClinGen gnomAD
rs1267313145 CA401659442	236	M>L		No	ClinGen TOPMed
CA401659446 rs1245548601	236	M>T		No	ClinGen TOPMed
rs1267313145 CA401659443	236	M>V		No	ClinGen TOPMed
CA401659451 rs1223804477	237	I>L		No	ClinGen gnomAD
rs959180600 CA295393384	237	I>M		No	ClinGen TOPMed
CA295393387 rs866542441	238	A>V		No	ClinGen gnomAD
rs1220777084 CA401659471	240	I>V		No	ClinGen gnomAD
rs750248338 CA295393398	241	T>M		No	ClinGen TOPMed gnomAD
CA401659523 rs1448674651	246	G>A		No	ClinGen gnomAD
CA401659522 rs1448674651	246	G>D		No	ClinGen gnomAD
rs1350693342 CA401659598	257	I>V		No	ClinGen TOPMed
rs1598481046 CA401659609	258	Y>S		No	ClinGen Ensembl
CA8867939 rs143415597	262	I>M		No	ClinGen 1000Genomes ESP ExAC TOPMed gnomAD
CA8867938 rs761730468	262	I>S		No	ClinGen ExAC gnomAD
rs780321607 CA295393628	263	E>K		No	ClinGen gnomAD
rs780321607 CA401659638	263	E>Q		No	ClinGen gnomAD
rs1390986435 CA401659655	265	L>R		No	ClinGen TOPMed
rs766563822	268	Q>=	Variant assessed as Somatic; 0.0 impact. [NCI-TCGA]	No	NCI-TCGA
CA295394076 rs1024764626 COSM1240594	271	R>Q	oesophagus [Cosmic]	No	ClinGen cosmic curated TOPMed
CA401660074 rs1216783286	274	R>K		No	ClinGen Ensembl
CA295394078 rs970583280	278	K>N		No	ClinGen TOPMed
CA401660137 rs1467164009	279	L>V		No	ClinGen TOPMed
rs751931801 CA8867967	281	I>T		No	ClinGen ExAC gnomAD
TCGA novel	283	R>Q	Variant assessed as Somatic; impact. [NCI-TCGA]	No	NCI-TCGA
rs779604534 CA8867969	285	V>I		No	ClinGen ExAC gnomAD
CA8867970 rs369187265	286	E>K		No	ClinGen ESP ExAC gnomAD
CA401660248 rs1328401362	287	K>T		No	ClinGen TOPMed
CA8867972 rs778541361	288	I>T		No	ClinGen ExAC gnomAD
rs748095422 CA8867973	289	D>G		No	ClinGen ExAC TOPMed gnomAD
CA8867974 rs375413487	293	A>T		No	ClinGen ESP ExAC TOPMed gnomAD
rs771147687 CA8867977	298	I>T		No	ClinGen ExAC gnomAD
CA295394101 rs765591852	299	E>K	Variant assessed as Somatic; impact. [NCI-TCGA]	No	ClinGen NCI-TCGA TOPMed
rs776689763 CA8867978	302	N>K		No	ClinGen ExAC gnomAD
CA8867979 rs759853185	303	P>L	Variant assessed as Somatic; 0.0 impact. [NCI-TCGA]	No	ClinGen ExAC NCI-TCGA TOPMed gnomAD
rs1442195189 CA401660466	304	H>Y		No	ClinGen TOPMed
CA8867981 rs752774827	305	P>R		No	ClinGen ExAC gnomAD
TCGA novel	307	I>S	Variant assessed as Somatic; impact. [NCI-TCGA]	No	NCI-TCGA
CA8867982 rs763389486	310	E>V		No	ClinGen ExAC gnomAD
rs764603167 CA8867983	311	M>I		No	ClinGen ExAC TOPMed gnomAD
rs1567998376 CA401660571	311	M>T		No	ClinGen Ensembl
CA401660587 rs1289319018	312	A>V		No	ClinGen gnomAD
rs1598485906 CA401660596	313	V>G		No	ClinGen Ensembl
rs751880836 CA8867984	313	V>I		No	ClinGen ExAC TOPMed gnomAD
CA401660602 rs1598485915	314	V>L		No	ClinGen Ensembl

No associated diseases with P04818

2 regional properties for P04818

Type	Name	Position	InterPro Accession
active_site	Thymidylate synthase, active site	175 - 203	IPR020940
domain	Thymidylate synthase/dCMP hydroxymethylase domain	32 - 313	IPR023451

Functions

		Description
EC Number	2.1.1.45	Methyltransferases
Subcellular Localization	Nucleus Cytoplasm Mitochondrion Mitochondrion matrix Mitochondrion inner membrane
PANTHER Family
PANTHER Subfamily
PANTHER Protein Class
PANTHER Pathway Category	No pathway information available

7 GO annotations of cellular component

Name	Definition
cytoplasm	The contents of a cell excluding the plasma membrane and nucleus, but including other subcellular structures.
cytosol	The part of the cytoplasm that does not contain organelles but which does contain other particulate matter, such as protein complexes.
mitochondrial inner membrane	The inner, i.e. lumen-facing, lipid bilayer of the mitochondrial envelope. It is highly folded to form cristae.
mitochondrial matrix	The gel-like material, with considerable fine structure, that lies in the matrix space, or lumen, of a mitochondrion. It contains the enzymes of the tricarboxylic acid cycle and, in some organisms, the enzymes concerned with fatty acid oxidation.
mitochondrion	A semiautonomous, self replicating organelle that occurs in varying numbers, shapes, and sizes in the cytoplasm of virtually all eukaryotic cells. It is notably the site of tissue respiration.
nucleolus	A small, dense body one or more of which are present in the nucleus of eukaryotic cells. It is rich in RNA and protein, is not bounded by a limiting membrane, and is not seen during mitosis. Its prime function is the transcription of the nucleolar DNA into 45S ribosomal-precursor RNA, the processing of this RNA into 5.8S, 18S, and 28S components of ribosomal RNA, and the association of these components with 5S RNA and proteins synthesized outside the nucleolus. This association results in the formation of ribonucleoprotein precursors; these pass into the cytoplasm and mature into the 40S and 60S subunits of the ribosome.
nucleus	A membrane-bounded organelle of eukaryotic cells in which chromosomes are housed and replicated. In most cells, the nucleus contains all of the cell's chromosomes except the organellar chromosomes, and is the site of RNA synthesis and processing. In some species, or in specialized cell types, RNA metabolism or DNA replication may be absent.

5 GO annotations of molecular function

Name	Definition
folic acid binding	Binding to folic acid, pteroylglutamic acid. Folic acid is widely distributed as a member of the vitamin B complex and is essential for the synthesis of purine and pyrimidines.
mRNA regulatory element binding translation repressor activity	Antagonizes the ribosome-mediated translation of mRNA into a polypeptide via direct binding (through a selective and non-covalent interaction) to nucleic acid.
protein homodimerization activity	Binding to an identical protein to form a homodimer.
sequence-specific mRNA binding	Binding to messenger RNA (mRNA) of a specific nucleotide composition or a specific sequence motif.
thymidylate synthase activity	Catalysis of the reaction: 5,10-methylenetetrahydrofolate + dUMP = 7,8-dihydrofolate + thymidylate.

22 GO annotations of biological process

Name	Definition
aging	A developmental process that is a deterioration and loss of function over time. Aging includes loss of functions such as resistance to disease, homeostasis, and fertility, as well as wear and tear. Aging includes cellular senescence, but is more inclusive. May precede death and may succeed developmental maturation (GO:0021700).
cartilage development	The process whose specific outcome is the progression of a cartilage element over time, from its formation to the mature structure. Cartilage elements are skeletal elements that consist of connective tissue dominated by extracellular matrix containing collagen type II and large amounts of proteoglycan, particularly chondroitin sulfate.
circadian rhythm	Any biological process in an organism that recurs with a regularity of approximately 24 hours.
developmental growth	The increase in size or mass of an entire organism, a part of an organism or a cell, where the increase in size or mass has the specific outcome of the progression of the organism over time from one condition to another.
DNA biosynthetic process	The biosynthetic process resulting in the formation of DNA.
dTMP biosynthetic process	The chemical reactions and pathways resulting in the formation of dTMP, deoxyribosylthymine monophosphate (2'-deoxyribosylthymine 5'-phosphate).
dTTP biosynthetic process	The chemical reactions and pathways resulting in the formation of dTTP, deoxyribosylthymine triphosphate.
intestinal epithelial cell maturation	The developmental process, independent of morphogenetic (shape) change, that is required for a columna/cuboidal epithelial cell of the intestine to attain its fully functional state. A columnar/cuboidal epithelial cell of the intestine mature as they migrate from the intestinal crypt to the villus.
liver regeneration	The regrowth of lost or destroyed liver.
methylation	The process in which a methyl group is covalently attached to a molecule.
negative regulation of translation	Any process that stops, prevents, or reduces the frequency, rate or extent of the chemical reactions and pathways resulting in the formation of proteins by the translation of mRNA or circRNA.
response to cytokine	Any process that results in a change in state or activity of a cell or an organism (in terms of movement, secretion, enzyme production, gene expression, etc.) as a result of a cytokine stimulus.
response to ethanol	Any process that results in a change in state or activity of a cell or an organism (in terms of movement, secretion, enzyme production, gene expression, etc.) as a result of an ethanol stimulus.
response to folic acid	Any process that results in a change in state or activity of a cell or an organism (in terms of movement, secretion, enzyme production, gene expression, etc.) as a result of a folic acid stimulus.
response to glucocorticoid	Any process that results in a change in state or activity of a cell or an organism (in terms of movement, secretion, enzyme production, gene expression, etc.) as a result of a glucocorticoid stimulus. Glucocorticoids are hormonal C21 corticosteroids synthesized from cholesterol with the ability to bind with the cortisol receptor and trigger similar effects. Glucocorticoids act primarily on carbohydrate and protein metabolism, and have anti-inflammatory effects.
response to organophosphorus	Any process that results in a change in state or activity of a cell or an organism (in terms of movement, secretion, enzyme production, gene expression, etc.) as a result of an organophosphorus stimulus. Organophosphorus is a compound containing phosphorus bound to an organic molecule; several organophosphorus compounds are used as insecticides, and they are highly toxic cholinesterase inhibitors.
response to progesterone	Any process that results in a change in state or activity of a cell or an organism (in terms of movement, secretion, enzyme production, gene expression, etc.) as a result of a progesterone stimulus.
response to toxic substance	Any process that results in a change in state or activity of a cell or an organism (in terms of movement, secretion, enzyme production, gene expression, etc.) as a result of a toxic stimulus.
response to vitamin A	Any process that results in a change in state or activity of a cell or an organism (in terms of movement, secretion, enzyme production, gene expression, etc.) as a result of a vitamin A stimulus.
response to xenobiotic stimulus	Any process that results in a change in state or activity of a cell or an organism (in terms of movement, secretion, enzyme production, gene expression, etc.) as a result of a stimulus from a xenobiotic, a compound foreign to the organim exposed to it. It may be synthesized by another organism (like ampicilin) or it can be a synthetic chemical.
tetrahydrofolate interconversion	The chemical reactions and pathways by which one-carbon (C1) units are transferred between tetrahydrofolate molecules, to synthesise other tetrahydrofolate molecules.
uracil metabolic process	The chemical reactions and pathways involving uracil, 2,4-dioxopyrimidine, one of the pyrimidine bases occurring in RNA, but not in DNA.

5 homologous proteins in AiPD

UniProt AC	Gene Name	Protein Name	Species	Evidence Code
O76511	Ts	Thymidylate synthase	Drosophila melanogaster (Fruit fly)	PR
O81395	DRTS	Bifunctional dihydrofolate reductase-thymidylate synthase	Zea mays (Maize)	PR
P07607	Tyms	Thymidylate synthase	Mus musculus (Mouse)	PR
P45352	Tyms	Thymidylate synthase	Rattus norvegicus (Rat)	PR
Q05763	THY-2	Bifunctional dihydrofolate reductase-thymidylate synthase 2	Arabidopsis thaliana (Mouse-ear cress)	PR

10	20	30	40	50	60
MPVAGSELPR	RPLPPAAQER	DAEPRPPHGE	LQYLGQIQHI	LRCGVRKDDR	TGTGTLSVFG
70	80	90	100	110	120
MQARYSLRDE	FPLLTTKRVF	WKGVLEELLW	FIKGSTNAKE	LSSKGVKIWD	ANGSRDFLDS
130	140	150	160	170	180
LGFSTREEGD	LGPVYGFQWR	HFGAEYRDME	SDYSGQGVDQ	LQRVIDTIKT	NPDDRRIIMC
190	200	210	220	230	240
AWNPRDLPLM	ALPPCHALCQ	FYVVNSELSC	QLYQRSGDMG	LGVPFNIASY	ALLTYMIAHI
250	260	270	280	290	300
TGLKPGDFIH	TLGDAHIYLN	HIEPLKIQLQ	REPRPFPKLR	ILRKVEKIDD	FKAEDFQIEG
310
YNPHPTIKME	MAV