P70266

PR
Gene name
Pfkfb1
Protein name
6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 1
Names
6PF-2-K/Fru-2,6-P2ase 1, PFK/FBPase 1, 6PF-2-K/Fru-2,6-P2ase liver isozyme
Species
Mus musculus (Mouse)
KEGG Pathway
mmu:18639
EC number
2.7.1.105: Phosphotransferases with an alcohol group as acceptor
Protein Class

Descriptions

The autoinhibited protein was predicted that may have potential autoinhibitory elements via cis-regPred.

Autoinhibitory domains (AIDs)

1-65 (Predicted disordered autoinhibitory region) PR

Target domain

Relief mechanism

Assay

cis-regPred

Accessory elements

No accessory elements

References

Autoinhibited structure

Activated structure

1 structures for P70266

Entry ID Method Resolution Chain Position Source
AF-P70266-F1 Predicted AlphaFoldDB

23 variants for P70266

Variant ID(s) Position Change Description Diseaes Association Provenance
rs3389578867 4 E>G No EVA
rs3389591110 23 S>N No EVA
rs3389572113 60 T>K No EVA
rs3389545860 81 Y>F No EVA
rs3389588552 147 A>T No EVA
rs3412267645 156 F>I No EVA
rs3389574318 163 D>N No EVA
rs3412132890 192 D>E No EVA
rs3412331019 203 N>Y No EVA
rs3389545889 208 D>N No EVA
rs3412720487 211 L>S No EVA
rs3389572045 212 D>H No EVA
rs3389574365 262 S>N No EVA
rs3410995816 269 R>H No EVA
rs3412267657 304 S>C No EVA
rs3389572086 321 E>K No EVA
rs3389578859 351 A>V No EVA
rs3409590579 392 C>* No EVA
rs3412232702 392 C>S No EVA
rs3412444653 403 Y>P No EVA
rs3389578848 429 A>V No EVA
rs3389528828 461 E>* No EVA
rs3389528828 461 E>K No EVA

No associated diseases with P70266

2 regional properties for P70266

Type Name Position InterPro Accession
active_site Phosphoglycerate/bisphosphoglycerate mutase, active site 256 - 265 IPR001345
domain 6-phosphofructo-2-kinase 31 - 251 IPR013079

Functions

Description
EC Number 2.7.1.105 Phosphotransferases with an alcohol group as acceptor
Subcellular Localization
PANTHER Family
PANTHER Subfamily
PANTHER Protein Class
PANTHER Pathway Category No pathway information available

2 GO annotations of cellular component

Name Definition
6-phosphofructo-2-kinase/fructose-2,6-biphosphatase complex A homodimeric, bifunctional enzyme complex which catalyzes the synthesis and degradation of fructose 2,6-bisphosphate, and is required for both glycolysis and gluconeogenesis.
cytosol The part of the cytoplasm that does not contain organelles but which does contain other particulate matter, such as protein complexes.

6 GO annotations of molecular function

Name Definition
6-phosphofructo-2-kinase activity Catalysis of the reaction: beta-D-fructose 6-phosphate + ATP = beta-D-fructose 2,6-bisphosphate + ADP + 2 H(+).
ATP binding Binding to ATP, adenosine 5'-triphosphate, a universally important coenzyme and enzyme regulator.
fructose-2,6-bisphosphate 2-phosphatase activity Catalysis of the reaction: D-fructose 2,6-bisphosphate + H2O = D-fructose-6-phosphate + phosphate.
fructose-6-phosphate binding Binding to fructose 6-phosphate.
identical protein binding Binding to an identical protein or proteins.
kinase binding Binding to a kinase, any enzyme that catalyzes the transfer of a phosphate group.

10 GO annotations of biological process

Name Definition
animal organ regeneration The regrowth of a lost or destroyed animal organ.
carbohydrate phosphorylation The process of introducing a phosphate group into a carbohydrate, any organic compound based on the general formula Cx(H2O)y.
fructose 2,6-bisphosphate metabolic process The chemical reactions and pathways involving fructose 2,6-bisphosphate. The D enantiomer is an important regulator of the glycolytic and gluconeogenic pathways. It inhibits fructose 1,6-bisphosphatase and activates phosphofructokinase.
fructose metabolic process The chemical reactions and pathways involving fructose, the ketohexose arabino-2-hexulose. Fructose exists in a open chain form or as a ring compound. D-fructose is the sweetest of the sugars and is found free in a large number of fruits and honey.
positive regulation of glucokinase activity Any process that activates or increases the frequency, rate or extent of glucokinase activity, the catalysis of the transfer of a phosphate group, usually from ATP, to a glucose molecule.
response to cAMP Any process that results in a change in state or activity of a cell or an organism (in terms of movement, secretion, enzyme production, gene expression, etc.) as a result of a cAMP (cyclic AMP, adenosine 3',5'-cyclophosphate) stimulus.
response to glucagon Any process that results in a change in state or activity of a cell or an organism (in terms of movement, secretion, enzyme production, gene expression, etc.) as a result of a glucagon stimulus.
response to glucocorticoid Any process that results in a change in state or activity of a cell or an organism (in terms of movement, secretion, enzyme production, gene expression, etc.) as a result of a glucocorticoid stimulus. Glucocorticoids are hormonal C21 corticosteroids synthesized from cholesterol with the ability to bind with the cortisol receptor and trigger similar effects. Glucocorticoids act primarily on carbohydrate and protein metabolism, and have anti-inflammatory effects.
response to insulin Any process that results in a change in state or activity of a cell or an organism (in terms of movement, secretion, enzyme production, gene expression, etc.) as a result of an insulin stimulus. Insulin is a polypeptide hormone produced by the islets of Langerhans of the pancreas in mammals, and by the homologous organs of other organisms.
response to starvation Any process that results in a change in state or activity of a cell or an organism (in terms of movement, secretion, enzyme production, gene expression, etc.) as a result of a starvation stimulus, deprivation of nourishment.

13 homologous proteins in AiPD

UniProt AC Gene Name Protein Name Species Evidence Code
P32604 FBP26 Fructose-2,6-bisphosphatase Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) PR
P26285 PFKFB2 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 2 Bos taurus (Bovine) PR
P49872 PFKFB1 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 1 Bos taurus (Bovine) PR
O60825 PFKFB2 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 2 Homo sapiens (Human) PR
Q16875 PFKFB3 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 3 Homo sapiens (Human) PR
Q16877 PFKFB4 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 4 Homo sapiens (Human) PR
P16118 PFKFB1 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 1 Homo sapiens (Human) PR
P70265 Pfkfb2 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 2 Mus musculus (Mouse) PR
Q6DTY7 Pfkfb4 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 4 Mus musculus (Mouse) PR
O35552 Pfkfb3 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 3 Rattus norvegicus (Rat) PR
P25114 Pfkfb4 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 4 Rattus norvegicus (Rat) PR
Q9JJH5 Pfkfb2 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 2 Rattus norvegicus (Rat) PR
P07953 Pfkfb1 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 1 Rattus norvegicus (Rat) PR
10 20 30 40 50 60
MSREMGELTQ TRLQKIWIPH SSSSSLLQRR RGSSIPQFTN SPTMVIMVGL PARGKTYIST
70 80 90 100 110 120
KLTRYLNWIG TPTKVFNLGQ YRREAVSYRN YEFFRPDNME AQLIRKQCAL AALKDVHKYL
130 140 150 160 170 180
SREEGHVAVF DATNTTRERR SLILQFAKEH GYKVFFIESI CNDPDIIAEN IKQVKLGSPD
190 200 210 220 230 240
YIDCDQEKVL EDFLKRIECY EINYQPLDEE LDSHLSYIKI FDVGTRYMVN RVQDHVQSRT
250 260 270 280 290 300
AYYLMNIHVT PRSIYLCRHG ESELNLRGRI GGDSGLSARG KQYAYALANF IRSQSISSLK
310 320 330 340 350 360
VWTSHMKRTI QTAEALGVPY EQWKALNEID AGVCEEMTYE EIQEHYPEEF ALRDQDKYRY
370 380 390 400 410 420
RYPKGESYED LVQRLEPVIM ELERQENVLV ICHQAVMRCL LAYFLDKSSD ELPYLKCPLH
430 440 450 460 470
TVLKLTPVAY GCRVESIYLN VEAVNTHRDK PENVDITREP EEALDTVPAH Y