AiPD: Autoinhibited Protein Database

P28676

Gene name	GCA (GCL)
Protein name	Grancalcin
Names
Species	Homo sapiens (Human)
KEGG Pathway	hsa:25801
EC number
Protein Class

Descriptions

The autoinhibited protein was predicted that may have potential autoinhibitory elements via cis-regPred.

Autoinhibitory domains (AIDs)

1-70 (Predicted disordered autoinhibitory region) PR

Target domain
Relief mechanism
Assay	cis-regPred

AID

1-70 (Predicted disordered autoinhibitory region)

Target domain

Relief mechanism

Assay

cis-regPred

Accessory elements

No accessory elements

References

Yeon JH et al. (2016) "Systems-wide Identification of cis-Regulatory Elements in Proteins", Cell Systems, 2, 89-100

Autoinhibited structure

Activated structure

5 structures for P28676

Entry ID	Method	Resolution	Chain	Position	Source
1F4O	X-ray	250 A	A/B	53-217	PDB
1F4Q	X-ray	190 A	A/B	53-217	PDB
1K94	X-ray	170 A	A/B	53-217	PDB
1K95	X-ray	190 A	A	53-217	PDB
AF-P28676-F1	Predicted				AlphaFoldDB

186 variants for P28676

Variant ID(s)	Position	Change	Description	Diseaes Association	Provenance
CA348989227 rs1207831687	2	A>S		No	ClinGen TOPMed
rs1441631066 CA348989230	2	A>V		No	ClinGen TOPMed
rs1380680973 CA348989253	3	Y>*		No	ClinGen TOPMed gnomAD
CA348989249 rs1384365254	3	Y>C		No	ClinGen TOPMed gnomAD
rs1326490816 CA348989266	4	P>A		No	ClinGen TOPMed
rs377430052 CA1934953	4	P>L		No	ClinGen ESP ExAC gnomAD
rs1243075701 CA348989297	5	G>*		No	ClinGen TOPMed gnomAD
CA348989298 rs1283916066	5	G>E		No	ClinGen gnomAD
rs969544407 CA59664107	6	Y>*		No	ClinGen TOPMed
rs1284468160 CA348989364	8	G>E		No	ClinGen gnomAD
rs768158188 CA1934955	8	G>R		No	ClinGen ExAC TOPMed gnomAD
rs981859315 CA59664132	9	G>V		No	ClinGen TOPMed
TCGA novel	11	G>R	Variant assessed as Somatic; impact. [NCI-TCGA]	No	NCI-TCGA
rs774079196 CA1934979	13	F>L		No	ClinGen ExAC TOPMed gnomAD
rs759501870 CA1934980	15	I>L		No	ClinGen ExAC gnomAD
CA348990521 rs759501870	15	I>V		No	ClinGen ExAC gnomAD
TCGA novel	16	Q>*	Variant assessed as Somatic; impact. [NCI-TCGA]	No	NCI-TCGA
rs767400119 CA1934981	16	Q>R		No	ClinGen ExAC TOPMed gnomAD
rs776817268 CA1934982	18	P>S		No	ClinGen ExAC gnomAD
CA348990615 rs1255868355	20	M>K		No	ClinGen gnomAD
rs761940724 CA1934983	21	Q>R		No	ClinGen ExAC gnomAD
rs765433325 CA1934984	22	M>V		No	ClinGen ExAC TOPMed gnomAD
CA1934985 rs750619966	23	G>E		No	ClinGen ExAC gnomAD
rs751929215 CA1934989	29	T>A		No	ClinGen ExAC gnomAD
rs1576283957 CA348990775	29	T>I		No	ClinGen Ensembl
CA59667114 rs903383504	30	G>D		No	ClinGen TOPMed gnomAD
COSM353038 rs903383504 CA348990797	30	G>V	lung [Cosmic]	No	ClinGen cosmic curated TOPMed gnomAD
rs748685566 CA1934992	31	P>R		No	ClinGen ExAC TOPMed gnomAD
CA348990806 rs1458703522	31	P>S		No	ClinGen gnomAD
CA348990823 rs1244793237	32	A>G		No	ClinGen TOPMed
rs1188863191 CA348990836	33	I>T		No	ClinGen gnomAD
CA348990828 rs1282026320	33	I>V		No	ClinGen gnomAD
CA59667148 rs1033141972	34	L>V		No	ClinGen Ensembl
rs1222017638 CA348990880	36	D>E		No	ClinGen gnomAD
rs1340258525 CA348990874	36	D>G		No	ClinGen TOPMed
rs774170639 CA1934997	36	D>N		No	ClinGen ExAC TOPMed gnomAD
rs201297392 CA1934998	38	Y>*		No	ClinGen 1000Genomes ExAC TOPMed gnomAD
CA59667185 rs140882466	39	S>P		No	ClinGen ESP TOPMed
CA348990940 rs1209328161	41	P>S		No	ClinGen TOPMed gnomAD
rs1293084545 CA348990960	42	A>P		No	ClinGen TOPMed
rs138275925 CA59667201	45	D>G		No	ClinGen ESP TOPMed
TCGA novel	49	S>L	Variant assessed as Somatic; impact. [NCI-TCGA]	No	NCI-TCGA
rs755258895 CA1935007	54	V>A		No	ClinGen ExAC gnomAD
rs766560685 CA1935005	54	V>L		No	ClinGen ExAC TOPMed gnomAD
rs766560685 CA1935006	54	V>M		No	ClinGen ExAC TOPMed gnomAD
rs767881330 CA1935008	56	T>A		No	ClinGen ExAC TOPMed gnomAD
TCGA novel	56	T>N	Variant assessed as Somatic; impact. [NCI-TCGA]	No	NCI-TCGA
CA1935009 rs773905255	57	Y>*		No	ClinGen ExAC gnomAD
rs756679204 CA1935011	61	V>F		No	ClinGen ExAC gnomAD
CA348991500 rs1194986306	62	A>T		No	ClinGen TOPMed
CA348991534 rs1558895320	63	G>E		No	ClinGen Ensembl
rs1558895341 CA348991562	64	Q>R		No	ClinGen Ensembl
CA348992787 rs1309960649	65	D>G		No	ClinGen gnomAD
CA59671181 rs903849548	65	D>Y		No	ClinGen TOPMed gnomAD
rs1355759760 CA348992831	67	E>D		No	ClinGen gnomAD
rs759809790 CA348992834	68	V>L		No	ClinGen ExAC gnomAD
rs759809790 CA1935026	68	V>M		No	ClinGen ExAC gnomAD
rs767862968 CA1935027	69	D>V		No	ClinGen ExAC gnomAD
rs1576290965 CA348992863	70	A>G		No	ClinGen Ensembl
rs79565841 CA1935030	74	Q>H		No	ClinGen 1000Genomes ESP ExAC TOPMed gnomAD
CA1935029 rs373720297	74	Q>R		No	ClinGen 1000Genomes ESP ExAC TOPMed gnomAD
CA348992934 rs1461195511	75	R>K		No	ClinGen TOPMed gnomAD
CA1935031 rs754396914	76	C>R		No	ClinGen ExAC TOPMed gnomAD
CA1935032 rs756668151	77	L>M		No	ClinGen ExAC gnomAD
CA348992973 rs1287713877	78	T>A		No	ClinGen gnomAD
rs1156325552 CA348992975	78	T>K		No	ClinGen gnomAD
rs1406553414 CA537098455	78	T>LME*		No	ClinGen gnomAD
CA1935033 rs778502564	79	Q>E		No	ClinGen ExAC gnomAD
rs17783344 CA1935034 VAR_048657	80	S>A		No	ClinGen UniProt 1000Genomes ESP ExAC TOPMed dbSNP gnomAD
rs1469160548 CA348993011	81	G>E		No	ClinGen gnomAD
rs1440950061 CA348993047	84	G>R		No	ClinGen TOPMed
rs746851226 CA1935038	86	Y>*		No	ClinGen ExAC gnomAD
CA1935037 rs758193923	86	Y>C		No	ClinGen ExAC TOPMed gnomAD
rs758193923 CA1935036	86	Y>F		No	ClinGen ExAC TOPMed gnomAD
rs1204493424 CA348993091	87	S>P		No	ClinGen TOPMed
CA1935039 rs768684664	88	P>S		No	ClinGen ExAC gnomAD
rs777124789 CA1935068	90	S>N		No	ClinGen ExAC TOPMed gnomAD
rs1223158704 CA348993927	93	T>I		No	ClinGen gnomAD
CA348993925 rs1223158704	93	T>S		No	ClinGen gnomAD
CA1935070 rs765825935	97	M>I		No	ClinGen ExAC gnomAD
COSM3961381 CA1935069 rs762216281	97	M>L	lung [Cosmic]	No	ClinGen cosmic curated ExAC gnomAD
rs1193997371 CA348993981	98	I>T		No	ClinGen TOPMed gnomAD
rs1158441479 CA348993998	100	M>V		No	ClinGen TOPMed
rs1474344000 CA348994025	102	D>H		No	ClinGen gnomAD
CA348994081 rs1274926193	104	D>A		No	ClinGen TOPMed
TCGA novel	104	D>N	Variant assessed as Somatic; impact. [NCI-TCGA]	No	NCI-TCGA
rs780108109 CA348994111	106	T>I		No	ClinGen ExAC gnomAD
rs780108109 CA1935082	106	T>K		No	ClinGen ExAC gnomAD
CA1935083 rs747231579	109	M>V		No	ClinGen ExAC gnomAD
CA348994150 rs1233059959 CA348994151	110	G>R		No	ClinGen TOPMed gnomAD
CA1935084 rs768984156	112	N>K		No	ClinGen ExAC gnomAD
rs376239356 CA59675008	115	K>I		No	ClinGen ESP ExAC TOPMed gnomAD
CA1935085 rs376239356	115	K>R		No	ClinGen ESP ExAC TOPMed gnomAD
rs748589021 CA1935086	117	L>V		No	ClinGen ExAC TOPMed gnomAD
CA348994252 rs1314290993	118	W>*		No	ClinGen gnomAD
rs1381824423 COSM302293 CA348994259	119	A>E	central_nervous_system [Cosmic]	No	ClinGen cosmic curated gnomAD
rs1446263061 CA348994312	124	W>C		No	ClinGen gnomAD
rs763567637 CA1935089	125	K>E		No	ClinGen ExAC TOPMed gnomAD
CA1935090 rs765921170	127	N>D		No	ClinGen ExAC gnomAD
CA1935092 rs574274580	129	M>I		No	ClinGen 1000Genomes ExAC TOPMed gnomAD
CA1935091 rs774061805	129	M>V		No	ClinGen ExAC gnomAD
CA1935093 rs767237823	131	V>L		No	ClinGen ExAC gnomAD
CA1935095 rs755886900	132	D>V		No	ClinGen ExAC gnomAD
rs1181518605 CA348994432	134	D>E		No	ClinGen TOPMed gnomAD
CA1935096 rs763887378	134	D>G		No	ClinGen ExAC TOPMed gnomAD
CA1935098 rs753704592	135	G>*		No	ClinGen ExAC gnomAD
CA1935097 rs753704592	135	G>R		No	ClinGen ExAC gnomAD
CA1935099 rs748003453	136	S>G		No	ClinGen ExAC gnomAD
rs1464188201 CA348994447	136	S>N		No	ClinGen TOPMed
CA1935100 rs751690544	138	T>A		No	ClinGen ExAC gnomAD
rs1353408961 CA348994483	140	E>K		No	ClinGen TOPMed
rs748342688 CA1935105	141	H>L		No	ClinGen ExAC
CA1935103 rs781436722	141	H>Y		No	ClinGen ExAC gnomAD
rs770137996 CA1935106	142	H>Q		No	ClinGen ExAC gnomAD
CA348994519 rs1336422978	142	H>R		No	ClinGen gnomAD
rs976980582 CA59675110	143	E>D		No	ClinGen gnomAD
rs957923900 CA59675103	143	E>Q		No	ClinGen TOPMed
CA348994559 rs1305447331	144	L>S		No	ClinGen gnomAD
rs563127943 CA1935108	145	R>C	Variant assessed as Somatic; 0.0 impact. [NCI-TCGA]	No	ClinGen 1000Genomes ExAC NCI-TCGA TOPMed gnomAD
CA1935109 rs374402814	145	R>H		No	ClinGen 1000Genomes ESP ExAC TOPMed gnomAD
CA348994579 rs374402814	145	R>L	Variant assessed as Somatic; 0.0 impact. [NCI-TCGA]	No	ClinGen 1000Genomes ESP ExAC NCI-TCGA TOPMed gnomAD
rs935167342 CA59675123	147	A>T		No	ClinGen Ensembl
CA348994637 rs1201551043	149	G>A		No	ClinGen gnomAD
rs773966108 CA1935110	149	G>C		No	ClinGen ExAC gnomAD
CA1935111 rs759045455	151	M>I		No	ClinGen ExAC gnomAD
CA59675130 rs776471360	151	M>T		No	ClinGen TOPMed
CA348995044 rs1199029177	152	G>V		No	ClinGen gnomAD
CA348995054 rs1424586458	153	Y>C		No	ClinGen gnomAD
CA348995053 rs1424586458	153	Y>F		No	ClinGen gnomAD
CA59676639 rs904134185	154	R>K		No	ClinGen TOPMed gnomAD
rs760359625 CA59676643	154	R>S		No	ClinGen ExAC TOPMed gnomAD
TCGA novel	154	R>W	Variant assessed as Somatic; impact. [NCI-TCGA]	No	NCI-TCGA
rs776380970 CA1935134	155	L>S		No	ClinGen ExAC gnomAD
CA348995095 rs1366951115	157	P>A		No	ClinGen gnomAD
rs548241509 CA1935135	159	T>I		No	ClinGen 1000Genomes ExAC gnomAD
rs750318325 CA1935139	161	T>A		No	ClinGen ExAC gnomAD
rs184430160 CA1935140	162	T>A		No	ClinGen 1000Genomes ExAC gnomAD
rs1006089442 CA59676658	162	T>I		No	ClinGen Ensembl
TCGA novel	162	T>N	Variant assessed as Somatic; impact. [NCI-TCGA]	No	NCI-TCGA
rs1558903683 CA348995161	163	I>V		No	ClinGen Ensembl
CA59676684 rs767630618	164	V>I		No	ClinGen ExAC TOPMed gnomAD
rs767630618 CA1935142	164	V>L		No	ClinGen ExAC TOPMed gnomAD
rs377112025 COSM1008382 CA1935143	166	R>C	Variant assessed as Somatic; 0.0 impact. endometrium [NCI-TCGA, Cosmic]	No	ClinGen cosmic curated 1000Genomes ESP ExAC NCI-TCGA TOPMed gnomAD
rs1576300319 CA348995195	166	R>H		No	ClinGen Ensembl
rs146161584 CA1935145	168	S>G		No	ClinGen 1000Genomes ESP ExAC TOPMed gnomAD
rs1221833922 CA348995228	169	K>E		No	ClinGen gnomAD
CA59676700 rs974996717	169	K>R		No	ClinGen Ensembl
rs754134384 CA1935146	171	G>D		No	ClinGen ExAC gnomAD
rs746379441 CA1935149	175	F>L		No	ClinGen ExAC gnomAD
rs955648964 CA59676732	176	D>G		No	ClinGen TOPMed
rs955648964 CA59676725	176	D>V		No	ClinGen TOPMed
CA59676752 rs896884022	177	D>H		No	ClinGen TOPMed gnomAD
CA348995330 rs896884022	177	D>N		No	ClinGen TOPMed gnomAD
CA1935151 rs779597767	178	Y>C		No	ClinGen ExAC gnomAD
CA348995347 rs779597767	178	Y>F		No	ClinGen ExAC gnomAD
rs1162317604 CA348995401	183	V>L		No	ClinGen gnomAD
rs1286729125 CA348995418	184	K>N		No	ClinGen gnomAD
rs367777675 CA1935154	184	K>T		No	ClinGen ESP ExAC TOPMed gnomAD
rs141572054 CA1935155	186	R>*		No	ClinGen 1000Genomes ESP ExAC TOPMed gnomAD
CA1935156 rs769512674	186	R>Q	Variant assessed as Somatic; 0.0 impact. [NCI-TCGA]	No	ClinGen ExAC NCI-TCGA TOPMed gnomAD
rs772948593 CA1935157	187	A>V		No	ClinGen ExAC gnomAD
CA1935162 rs760787628	189	T>I		No	ClinGen ExAC gnomAD
CA1935163 rs764280974	190	D>N		No	ClinGen ExAC gnomAD
rs1406813775 CA348995554	193	R>W		No	ClinGen gnomAD
rs1433361774 CA348995565	194	K>E		No	ClinGen gnomAD
CA1935180 rs558963969	194	K>R		No	ClinGen 1000Genomes ExAC TOPMed gnomAD
CA348995599 rs1185253678	196	D>E		No	ClinGen TOPMed
rs774097597 CA1935182	198	L>S		No	ClinGen ExAC gnomAD
CA348995615 rs1332036724	199	Q>K		No	ClinGen gnomAD
rs1356138320 CA348995623	200	Q>K		No	ClinGen TOPMed gnomAD
CA59677135 rs889443052	200	Q>R		No	ClinGen TOPMed
CA348995631 rs1332971486	201	G>R		No	ClinGen TOPMed
rs558346557 CA348995651	203	A>G		No	ClinGen ExAC TOPMed gnomAD
rs558346557 CA1935183	203	A>V		No	ClinGen ExAC TOPMed gnomAD
rs764260531 CA1935184	206	I>L		No	ClinGen ExAC TOPMed gnomAD
CA1935186 rs368854743	206	I>M		No	ClinGen ExAC TOPMed gnomAD
rs764260531 CA1935185	206	I>V		No	ClinGen ExAC TOPMed gnomAD
rs765421255 CA1935187	207	Y>H		No	ClinGen ExAC gnomAD
rs1219634675 CA348995704	208	D>G		No	ClinGen gnomAD
CA1935188 rs146185329	208	D>N		No	ClinGen ESP ExAC TOPMed gnomAD
CA1935190 COSM1008383 rs766783966	209	D>N	Variant assessed as Somatic; 0.0 impact. endometrium [NCI-TCGA, Cosmic]	No	ClinGen cosmic curated ExAC NCI-TCGA gnomAD
rs1161692599 CA348995801	212	Q>H		No	ClinGen gnomAD
rs1365214851 CA348995805	213	G>C		No	ClinGen gnomAD
CA348995809 rs1401465572	213	G>D		No	ClinGen gnomAD
rs767971090 CA1935212	214	T>A		No	ClinGen ExAC gnomAD
CA1935213 rs753222449	215	M>V		No	ClinGen ExAC gnomAD

No associated diseases with P28676

3 regional properties for P28676

Type	Name	Position	InterPro Accession
domain	EF-hand domain	64 - 121	IPR002048-1
domain	EF-hand domain	119 - 154	IPR002048-2
binding_site	EF-Hand 1, calcium-binding site	132 - 144	IPR018247

Functions

		Description
EC Number
Subcellular Localization	Cytoplasm Cytoplasmic granule membrane ; Peripheral membrane protein; Cytoplasmic side	Primarily cytosolic in the absence of calcium or magnesium ions Relocates to granules and other membranes in response to elevated calcium and magnesium levels
PANTHER Family
PANTHER Subfamily
PANTHER Protein Class
PANTHER Pathway Category	No pathway information available

6 GO annotations of cellular component

Name	Definition
azurophil granule lumen	The volume enclosed by the membrane of an azurophil granule, a primary lysosomal granule found in neutrophil granulocytes that contains a wide range of hydrolytic enzymes and is released into the extracellular fluid.
cytoplasm	The contents of a cell excluding the plasma membrane and nucleus, but including other subcellular structures.
cytosol	The part of the cytoplasm that does not contain organelles but which does contain other particulate matter, such as protein complexes.
extracellular exosome	A vesicle that is released into the extracellular region by fusion of the limiting endosomal membrane of a multivesicular body with the plasma membrane. Extracellular exosomes, also simply called exosomes, have a diameter of about 40-100 nm.
extracellular region	The space external to the outermost structure of a cell. For cells without external protective or external encapsulating structures this refers to space outside of the plasma membrane. This term covers the host cell environment outside an intracellular parasite.
plasma membrane	The membrane surrounding a cell that separates the cell from its external environment. It consists of a phospholipid bilayer and associated proteins.

3 GO annotations of molecular function

Name	Definition
calcium ion binding	Binding to a calcium ion (Ca2+).
protein heterodimerization activity	Binding to a nonidentical protein to form a heterodimer.
protein homodimerization activity	Binding to an identical protein to form a homodimer.

1 GO annotations of biological process

Name	Definition
membrane fusion	The membrane organization process that joins two lipid bilayers to form a single membrane.

6 homologous proteins in AiPD

UniProt AC	Gene Name	Protein Name	Species	Evidence Code
Q6MZZ7	CAPN13	Calpain-13	Homo sapiens (Human)	PR
Q9UBV8	PEF1	Peflin	Homo sapiens (Human)	PR
Q3UW68	Capn13	Calpain-13	Mus musculus (Mouse)	PR
Q8BFY6	Pef1	Peflin	Mus musculus (Mouse)	PR
Q5BK10	Capn13	Calpain-13	Rattus norvegicus (Rat)	PR
Q641Z8	Pef1	Peflin	Rattus norvegicus (Rat)	PR

10	20	30	40	50	60
MAYPGYGGGF	GNFSIQVPGM	QMGQPVPETG	PAILLDGYSG	PAYSDTYSSA	GDSVYTYFSA
70	80	90	100	110	120
VAGQDGEVDA	EELQRCLTQS	GINGTYSPFS	LETCRIMIAM	LDRDHTGKMG	FNAFKELWAA
130	140	150	160	170	180
LNAWKENFMT	VDQDGSGTVE	HHELRQAIGL	MGYRLSPQTL	TTIVKRYSKN	GRIFFDDYVA
190	200	210
CCVKLRALTD	FFRKRDHLQQ	GSANFIYDDF	LQGTMAI