O70157
PR
Gene name |
Top3a (Top3) |
Protein name |
DNA topoisomerase 3-alpha |
Names |
DNA topoisomerase III alpha |
Species |
Mus musculus (Mouse) |
KEGG Pathway |
mmu:21975 |
EC number |
5.6.2.1: Enzymes altering nucleic acid conformation |
Protein Class |
|
Descriptions
The autoinhibited protein was predicted that may have potential autoinhibitory elements via cis-regPred.
Autoinhibitory domains (AIDs)
Target domain |
|
Relief mechanism |
|
Assay |
cis-regPred |
Accessory elements
No accessory elements
Autoinhibited structure
Activated structure
1 structures for O70157
| Entry ID | Method | Resolution | Chain | Position | Source |
|---|---|---|---|---|---|
| AF-O70157-F1 | Predicted | AlphaFoldDB |
57 variants for O70157
| Variant ID(s) | Position | Change | Description | Diseaes Association | Provenance |
|---|---|---|---|---|---|
| rs241887321 | 13 | H>Q | No | EVA | |
| rs3389157721 | 37 | L>V | No | EVA | |
| rs3389102653 | 44 | D>E | No | EVA | |
| rs3389162818 | 77 | L>P | No | EVA | |
| rs3402300201 | 111 | L>P | No | EVA | |
| rs3389165001 | 132 | T>N | No | EVA | |
| rs3389166106 | 136 | E>D | No | EVA | |
| rs240593895 | 166 | A>T | No | EVA | |
| rs3402356764 | 190 | C>R | No | EVA | |
| rs3389177168 | 196 | P>R | No | EVA | |
| rs3389171273 | 214 | I>V | No | EVA | |
| rs3389171287 | 250 | G>* | No | EVA | |
| rs3389173400 | 280 | T>S | No | EVA | |
| rs3401072766 | 304 | M>I | No | EVA | |
| rs3389177139 | 379 | L>S | No | EVA | |
| rs3389166101 | 391 | A>P | No | EVA | |
| rs3389151199 | 408 | S>I | No | EVA | |
| rs3389177181 | 410 | S>Y | No | EVA | |
| rs259071846 | 425 | G>A | No | EVA | |
| rs47853877 | 425 | G>S | No | EVA | |
| rs3389175672 | 457 | E>* | No | EVA | |
| rs3389161340 | 467 | A>T | No | EVA | |
| rs3389173439 | 470 | L>R | No | EVA | |
| rs3389138745 | 476 | N>S | No | EVA | |
| rs3389126623 | 530 | H>Y | No | EVA | |
| rs3402356740 | 548 | R>W | No | EVA | |
| rs3402293024 | 549 | M>L | No | EVA | |
| rs3389173483 | 571 | G>S | No | EVA | |
| rs3389171300 | 583 | D>E | No | EVA | |
| rs3389151133 | 614 | K>M | No | EVA | |
| rs3389165021 | 664 | D>E | No | EVA | |
| rs3389102718 | 664 | D>G | No | EVA | |
| rs232465818 | 680 | M>V | No | EVA | |
| rs51299671 | 728 | A>T | No | EVA | |
| rs3389134744 | 737 | I>T | No | EVA | |
| rs48135735 | 761 | Q>H | No | EVA | |
| rs26971987 | 781 | N>S | No | EVA | |
| rs3389175714 | 788 | N>Y | No | EVA | |
| rs244858664 | 792 | R>G | No | EVA | |
| rs215120009 | 798 | A>S | No | EVA | |
| rs252813547 | 800 | A>T | No | EVA | |
| rs233622691 | 810 | S>N | No | EVA | |
| rs3411059825 | 829 | Q>L | No | EVA | |
| rs243774875 | 842 | N>S | No | EVA | |
| rs46144596 | 855 | H>N | No | EVA | |
| rs3389138800 | 865 | A>V | No | EVA | |
| rs3402275431 | 881 | S>C | No | EVA | |
| rs3389157674 | 888 | S>I | No | EVA | |
| rs46779599 | 897 | T>A | No | EVA | |
| rs47051744 | 898 | P>S | No | EVA | |
| rs3389162543 | 915 | P>S | No | EVA | |
| rs3389173437 | 930 | Q>E | No | EVA | |
| rs3389161298 | 937 | W>C | No | EVA | |
| rs3389175704 | 950 | P>R | No | EVA | |
| rs3389161283 | 957 | G>* | No | EVA | |
| rs263288118 | 998 | F>L | No | EVA | |
| rs3389162550 | 999 | C>G | No | EVA |
No associated diseases with O70157
10 regional properties for O70157
| Type | Name | Position | InterPro Accession |
|---|---|---|---|
| domain | DNA topoisomerase, type IA, domain 2 | 172 - 269 | IPR003601 |
| domain | DNA topoisomerase, type IA, DNA-binding domain | 315 - 569 | IPR003602 |
| domain | TOPRIM domain | 35 - 181 | IPR006171 |
| domain | Zinc finger, GRF-type | 813 - 854 | IPR010666-1 |
| domain | Zinc finger, GRF-type | 898 - 941 | IPR010666-2 |
| domain | DNA topoisomerase, type IA, central | 143 - 156 | IPR013497-1 |
| domain | DNA topoisomerase, type IA, central | 196 - 617 | IPR013497-2 |
| domain | DNA topoisomerase, type IA, zn finger | 655 - 693 | IPR013498 |
| active_site | DNA topoisomerase, type IA, active site | 352 - 366 | IPR023406 |
| domain | DNA topoisomerase 3-like, TOPRIM domain | 35 - 193 | IPR034144 |
Functions
| Description | ||
|---|---|---|
| EC Number | 5.6.2.1 | Enzymes altering nucleic acid conformation |
| Subcellular Localization |
|
|
| PANTHER Family | ||
| PANTHER Subfamily | ||
| PANTHER Protein Class | ||
| PANTHER Pathway Category | No pathway information available | |
5 GO annotations of cellular component
| Name | Definition |
|---|---|
| mitochondrial matrix | The gel-like material, with considerable fine structure, that lies in the matrix space, or lumen, of a mitochondrion. It contains the enzymes of the tricarboxylic acid cycle and, in some organisms, the enzymes concerned with fatty acid oxidation. |
| nucleoplasm | That part of the nuclear content other than the chromosomes or the nucleolus. |
| nucleus | A membrane-bounded organelle of eukaryotic cells in which chromosomes are housed and replicated. In most cells, the nucleus contains all of the cell's chromosomes except the organellar chromosomes, and is the site of RNA synthesis and processing. In some species, or in specialized cell types, RNA metabolism or DNA replication may be absent. |
| PML body | A class of nuclear body; they react against SP100 auto-antibodies (PML, promyelocytic leukemia); cells typically contain 10-30 PML bodies per nucleus; alterations in the localization of PML bodies occurs after viral infection. |
| RecQ family helicase-topoisomerase III complex | A complex containing a RecQ family helicase and a topoisomerase III homologue (a member of the topoisomerase type IA subfamily); may also include one or more additional proteins; conserved from E. coli to human. |
4 GO annotations of molecular function
| Name | Definition |
|---|---|
| DNA topoisomerase activity | Catalysis of the transient cleavage and passage of individual DNA strands or double helices through one another, resulting a topological transformation in double-stranded DNA. |
| DNA topoisomerase type I (single strand cut, ATP-independent) activity | Catalysis of a DNA topological transformation by transiently cleaving one DNA strand at a time to allow passage of another strand; changes the linking number by +1 per catalytic cycle. |
| single-stranded DNA binding | Binding to single-stranded DNA. |
| zinc ion binding | Binding to a zinc ion (Zn). |
5 GO annotations of biological process
| Name | Definition |
|---|---|
| chromosome separation | The cell cycle process in which paired chromosomes are detached from each other. Chromosome separation begins with the release of cohesin complexes from chromosomes; in budding yeast, this includes the cleavage of cohesin complexes along the chromosome arms, followed by the separation of the centromeric regions. Chromosome separation also includes formation of chromatid axes mediated by condensins, and ends with the disentangling of inter-sister catenation catalyzed by topoisomerase II (topo II). |
| DNA topological change | The process in which a transformation is induced in the topological structure of a double-stranded DNA helix, resulting in a change in linking number. |
| double-strand break repair via homologous recombination | The error-free repair of a double-strand break in DNA in which the broken DNA molecule is repaired using homologous sequences. A strand in the broken DNA searches for a homologous region in an intact chromosome to serve as the template for DNA synthesis. The restoration of two intact DNA molecules results in the exchange, reciprocal or nonreciprocal, of genetic material between the intact DNA molecule and the broken DNA molecule. |
| mitochondrial DNA metabolic process | The chemical reactions and pathways involving mitochondrial DNA. |
| resolution of recombination intermediates | The cleavage and rejoining of intermediates, such as Holliday junctions, formed during DNA recombination to produce two intact molecules in which genetic material has been exchanged. |
5 homologous proteins in AiPD
| UniProt AC | Gene Name | Protein Name | Species | Evidence Code |
|---|---|---|---|---|
| P13099 | TOP3 | DNA topoisomerase 3 | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) | PR |
| Q13472 | TOP3A | DNA topoisomerase 3-alpha | Homo sapiens (Human) | PR |
| Q9Z321 | Top3b | DNA topoisomerase 3-beta-1 | Mus musculus (Mouse) | PR |
| C7J0A2 | TOP3A | DNA topoisomerase 3-alpha | Oryza sativa subsp japonica (Rice) | PR |
| Q9LVP1 | TOP3A | DNA topoisomerase 3-alpha | Arabidopsis thaliana (Mouse-ear cress) | PR |
| 10 | 20 | 30 | 40 | 50 | 60 |
| MIFPVTLLAF | QWHRRPGGRA | LSRAAMEVAF | RGVRKVLCVA | EKNDAAKGIA | DLLSNGRMRR |
| 70 | 80 | 90 | 100 | 110 | 120 |
| KEGLSKFNKI | YEFDYHLYGQ | NVTMIMTSVS | GHLLAHDFQM | QFRKWQSCNP | LVLFEAEIEK |
| 130 | 140 | 150 | 160 | 170 | 180 |
| YCPENFIDIK | KTLERETHHC | QALVIWTDCD | REGENIGFEI | IHVCKAVKPN | LRVLRARFSE |
| 190 | 200 | 210 | 220 | 230 | 240 |
| ITPHAVRTAC | ENLTEPDQRV | SDAVDVRQEL | DLRIGAAFTR | FQTLRLQRIF | PEVLAEQLIS |
| 250 | 260 | 270 | 280 | 290 | 300 |
| YGSCQFPTLG | FVVERFKAIQ | AFVPEVFHKI | KVTHDHKDGT | VEFNWKRYRL | FNHTACLVLY |
| 310 | 320 | 330 | 340 | 350 | 360 |
| QLCMEDPMAT | VVEVRSKPKS | KWRPQALDTV | ELEKLASRKL | RINAKETMRI | AEKLYTQGYI |
| 370 | 380 | 390 | 400 | 410 | 420 |
| SYPRTETNIF | PKDLNLVALV | EQQTVDPHWG | AFAQTILERG | GPTPRNGSKS | DQAHPPIHPT |
| 430 | 440 | 450 | 460 | 470 | 480 |
| KYTSGLQGDD | RRLYEFIVRH | FLACCSQDAQ | GQETTVEIDI | AQERFVAHGL | IILARNYLDV |
| 490 | 500 | 510 | 520 | 530 | 540 |
| YPYDHWSDKL | LPVYEQGSHF | QPSTVEMVDG | ETSPPQLLTE | ADLIALMEKH | GIGTDATHAE |
| 550 | 560 | 570 | 580 | 590 | 600 |
| HIETIKARMY | VGLTSDKRFL | PGHLGMGLVE | GYDSMGYEMS | KPDLRAELEA | DLKLICEGKK |
| 610 | 620 | 630 | 640 | 650 | 660 |
| DKFQVLRQQV | QKYKQVFIEA | VAKAKKLDEA | LSQYLGERTE | MAQQEEIYPA | MPEPVRKCPQ |
| 670 | 680 | 690 | 700 | 710 | 720 |
| CNKDMVLKTK | KSGGFYLSCM | GFPECRSAVW | FPDSVLEASR | DNSVCSVCQP | PPVYRLKLKF |
| 730 | 740 | 750 | 760 | 770 | 780 |
| KRGSLPPAMP | LEFVGCIGGC | DETLKEIFGL | RFPRALPRAS | QPSGHLQASQ | ALNRMDSSQH |
| 790 | 800 | 810 | 820 | 830 | 840 |
| NLSQPLVNRH | TRPSKTVAQA | LLPPTTAGES | NSVTCNCGRE | AVLLTVRKQG | PNQGRHFYKC |
| 850 | 860 | 870 | 880 | 890 | 900 |
| SNGDCNFFLW | ADSSHSTGGG | TPTSASGPPG | SSVGCPSSVG | SHMDGFGSLG | SDSDGGTPCL |
| 910 | 920 | 930 | 940 | 950 | 960 |
| CGQPAVTRTV | QKDGPNKGRQ | FHTCAKPREQ | QCGFFQWVDE | NVAPGSFAAP | AWPGGRGKAQ |
| 970 | 980 | 990 | 1000 | ||
| RPEAASKRPR | AGSSDAGSTV | KKPRKCSLCH | QPGHTRTFCP | QNR |