E1BGN7

PR
Gene name
CGAS
Protein name
Cyclic GMP-AMP synthase
Names
cGAMP synthase, cGAS, 2'3'-cGAMP synthase
Species
Bos taurus (Bovine)
KEGG Pathway
EC number
2.7.7.86: Nucleotidyltransferases
Protein Class

Descriptions

The autoinhibited protein was predicted that may have potential autoinhibitory elements via cis-regPred.

Autoinhibitory domains (AIDs)

53-168 (Predicted disordered autoinhibitory region) PR

Target domain

Relief mechanism

Assay

cis-regPred

Accessory elements

No accessory elements

References

Autoinhibited structure

Activated structure

1 structures for E1BGN7

Entry ID Method Resolution Chain Position Source
AF-E1BGN7-F1 Predicted AlphaFoldDB

No variants for E1BGN7

Variant ID(s) Position Change Description Diseaes Association Provenance
No variants for E1BGN7

No associated diseases with E1BGN7

2 regional properties for E1BGN7

Type Name Position InterPro Accession
domain Mab-21-like, nucleotidyltransferase domain 203 - 380 IPR046903
domain Mab-21-like, HhH/H2TH-like domain 396 - 478 IPR046906

Functions

Description
EC Number 2.7.7.86 Nucleotidyltransferases
Subcellular Localization
  • Nucleus
  • Chromosome
  • Cell membrane ; Peripheral membrane protein
  • Cytoplasm, cytosol
  • Mainly localizes in the nucleus, and at low level in the cytosol (By similarity)
  • On chromosomes, enriched on centromeric satellite and LINE DNA repeat elements
  • Exported from the nucleus to the cytosol in a XPO1/CRM1 via the nuclear export signal in response to DNA stimulation (By similarity)
  • Outside the nucleus, localizes at the cell membrane as a peripheral membrane protein in resting conditions: association to the cell membrane is mediated via binding to phosphatidylinositol 4,5-bisphosphate (PtdIns(4,5)P2) (By similarity)
  • Localization at the cell membrane is required to limit the recognition of self-DNA
  • Following detection of double-stranded DNA (dsDNA), released from the cell membrane into the cytosol in order to signal
  • Upon transfection with dsDNA forms punctate structures that co-localize with DNA and Beclin-1 (BECN1)
  • Phosphorylation at Tyr-204 promotes cytosolic retention
  • In response to translation stress, translocates to the cytosol and associates with collided ribosomes (By similarity)
PANTHER Family
PANTHER Subfamily
PANTHER Protein Class
PANTHER Pathway Category No pathway information available

4 GO annotations of cellular component

Name Definition
cytosol The part of the cytoplasm that does not contain organelles but which does contain other particulate matter, such as protein complexes.
nucleus A membrane-bounded organelle of eukaryotic cells in which chromosomes are housed and replicated. In most cells, the nucleus contains all of the cell's chromosomes except the organellar chromosomes, and is the site of RNA synthesis and processing. In some species, or in specialized cell types, RNA metabolism or DNA replication may be absent.
plasma membrane The membrane surrounding a cell that separates the cell from its external environment. It consists of a phospholipid bilayer and associated proteins.
site of double-strand break A region of a chromosome at which a DNA double-strand break has occurred. DNA damage signaling and repair proteins accumulate at the lesion to respond to the damage and repair the DNA to form a continuous DNA helix.

12 GO annotations of molecular function

Name Definition
2',3'-cyclic GMP-AMP synthase activity Catalysis of the reaction: ATP + GTP = 2 diphosphate + cyclic G-P(2'-5')A-P(3'-5') (cyclic 2',3' GAMP).
ATP binding Binding to ATP, adenosine 5'-triphosphate, a universally important coenzyme and enzyme regulator.
chromatin binding Binding to chromatin, the network of fibers of DNA, protein, and sometimes RNA, that make up the chromosomes of the eukaryotic nucleus during interphase.
DNA binding Any molecular function by which a gene product interacts selectively and non-covalently with DNA (deoxyribonucleic acid).
double-stranded DNA binding Binding to double-stranded DNA.
GTP binding Binding to GTP, guanosine triphosphate.
metal ion binding Binding to a metal ion.
molecular condensate scaffold activity Binding and bringing together two or more macromolecules in contact, permitting those molecules to organize as a molecular condensate.
nucleosome binding Binding to a nucleosome, a complex comprised of DNA wound around a multisubunit core and associated proteins, which forms the primary packing unit of DNA into higher order structures.
phosphatidylinositol-4,5-bisphosphate binding Binding to phosphatidylinositol-4,5-bisphosphate, a derivative of phosphatidylinositol in which the inositol ring is phosphorylated at the 4' and 5' positions.
poly-ADP-D-ribose modification-dependent protein binding Binding to a protein upon poly-ADP-ribosylation of the target protein.
protein homodimerization activity Binding to an identical protein to form a homodimer.

12 GO annotations of biological process

Name Definition
activation of innate immune response Any process that initiates an innate immune response. Innate immune responses are defense responses mediated by germline encoded components that directly recognize components of potential pathogens. Examples of this process include activation of the hypersensitive response of Arabidopsis thaliana and activation of any NOD or TLR signaling pathway in vertebrate species.
cAMP-mediated signaling Any intracellular signal transduction in which the signal is passed on within the cell via cyclic AMP (cAMP). Includes production of cAMP, and downstream effectors that further transmit the signal within the cell.
cellular response to DNA damage stimulus Any process that results in a change in state or activity of a cell (in terms of movement, secretion, enzyme production, gene expression, etc.) as a result of a stimulus indicating damage to its DNA from environmental insults or errors during metabolism.
cellular response to exogenous dsRNA Any process that results in a change in state or activity of a cell (in terms of movement, secretion, enzyme production, gene expression, etc.) as a result of an exogenous double-stranded RNA stimulus.
defense response to virus Reactions triggered in response to the presence of a virus that act to protect the cell or organism.
DNA repair The process of restoring DNA after damage. Genomes are subject to damage by chemical and physical agents in the environment (e.g. UV and ionizing radiations, chemical mutagens, fungal and bacterial toxins, etc.) and by free radicals or alkylating agents endogenously generated in metabolism. DNA is also damaged because of errors during its replication. A variety of different DNA repair pathways have been reported that include direct reversal, base excision repair, nucleotide excision repair, photoreactivation, bypass, double-strand break repair pathway, and mismatch repair pathway.
innate immune response Innate immune responses are defense responses mediated by germline encoded components that directly recognize components of potential pathogens.
negative regulation of double-strand break repair via homologous recombination Any process that stops, prevents, or reduces the frequency, rate or extent of double-strand break repair via homologous recombination.
paracrine signaling The transfer of information from one cell to another, where the signal travels from the signal-producing cell to the receiving cell by passive diffusion or bulk flow in intercellular fluid. The signaling cell and the receiving cell are usually in the vicinity of each other.
positive regulation of cellular senescence Any process that activates or increases the frequency, rate or extent of cellular senescence.
positive regulation of defense response to virus by host Any host process that results in the promotion of antiviral immune response mechanisms, thereby limiting viral replication.
positive regulation of type I interferon production Any process that activates or increases the frequency, rate, or extent of type I interferon production. Type I interferons include the interferon-alpha, beta, delta, episilon, zeta, kappa, tau, and omega gene families.

4 homologous proteins in AiPD

UniProt AC Gene Name Protein Name Species Evidence Code
Q8N884 CGAS Cyclic GMP-AMP synthase Homo sapiens (Human) PR
Q8C525 Mb21d2 Nucleotidyltransferase MB21D2 Mus musculus (Mouse) PR
Q8C6L5 Cgas Cyclic GMP-AMP synthase Mus musculus (Mouse) PR
I3LM39 CGAS Cyclic GMP-AMP synthase Sus scrofa (Pig) PR
10 20 30 40 50 60
MAPPRRKATR KASETASGVS APCVEGGLSA EPSEPAAVPE APRPGARRCG AAGASGSRRE
70 80 90 100 110 120
KSRLDPREKP QVRARAARAE DQAEGPAAPT ADAEPPAAPG HSLPRASTRS RGTASSARAR
130 140 150 160 170 180
RPQSGPPEGP GLGPRAPSPH LGRREEAPGA WKPRAVLEKL KLSRQEISVA AEVVNRLGDH
190 200 210 220 230 240
LLRRLNSRES EFKGVDLLRT GSYYERVKIS APNEFDLMFT LEVPRIQLEE YCNSSAHYFV
250 260 270 280 290 300
KFKRNPKGSP LDQFLEGGIL SASKMLFKFR KIIKEEIKHI EDTDVIMERK KRGSPAVTLL
310 320 330 340 350 360
IRKPREISVD IILALESKSS WPASTQKGLP ISNWLGTKVK DNLKRQPFYL VPKHAKEGSL
370 380 390 400 410 420
FQEETWRLSF SHIEKAILTN HGQTKTCCET EGVKCCRKEC LKLMKYLLEQ LKKKFGKQRG
430 440 450 460 470 480
LDKFCSYHVK TAFLHVCTQN PHDSWWLYKD LELCFDNCVT YFLQCLKTEH LEHYFIPDVL
490
SKQIEYEQNN GFPVFDEF