AiPD: Autoinhibited Protein Database

Gene name	At3g13930 (MDC16.5)
Protein name	Dihydrolipoyllysine-residue acetyltransferase component 2 of pyruvate dehydrogenase complex, mitochondrial
Names	Dihydrolipoamide S-acetyltransferase component 2 of pyruvate dehydrogenase complex, Pyruvate dehydrogenase complex component E2 2, PDC-E2 2, PDCE2 2
Species	Arabidopsis thaliana (Mouse-ear cress)
KEGG Pathway	ath:AT3G13930
EC number	2.3.1.12: Transferring groups other than amino-acyl groups
Protein Class

The autoinhibited protein was predicted that may have potential autoinhibitory elements via cis-regPred.

43-349 (Predicted disordered autoinhibitory region) PR

43-349 (Predicted disordered autoinhibitory region)

cis-regPred

No accessory elements

Entry ID	Method	Resolution	Chain	Position	Source
AF-Q8RWN9-F1	Predicted				AlphaFoldDB

Variant ID(s)	Position	Change	Diseaes Association	Provenance
tmp_3_4596731_A_C	35	H>P	No	1000Genomes
ENSVATH02141860	46	K>E	No	1000Genomes
ENSVATH05825981	46	K>R	No	1000Genomes
ENSVATH05825982	63	G>D	No	1000Genomes
ENSVATH10663839	69	S>I	No	1000Genomes
ENSVATH00322060	73	T>K	No	1000Genomes
ENSVATH10663840	82	M>L	No	1000Genomes
tmp_3_4597255_T_A	88	F>Y	No	1000Genomes
ENSVATH05825983	89	K>I	No	1000Genomes
ENSVATH05825988	172	E>D	No	1000Genomes
tmp_3_4598210_A_C	199	Y>S	No	1000Genomes
ENSVATH00322069	201	P>A	No	1000Genomes
ENSVATH10663861	210	P>S	No	1000Genomes
ENSVATH05825990	214	P>L	No	1000Genomes
tmp_3_4598287_G_C	225	E>Q	No	1000Genomes
ENSVATH13914235	229	S>T	No	1000Genomes
tmp_3_4598324_A_T	237	K>M	No	1000Genomes
ENSVATH00322070	242	P>H	No	1000Genomes
ENSVATH10663862	246	R>C	No	1000Genomes
tmp_3_4598360_C_T	249	A>V	No	1000Genomes
tmp_3_4598533_G_A	277	V>M	No	1000Genomes
ENSVATH02141865	327	F>L	No	1000Genomes
ENSVATH05825993	341	T>S	No	1000Genomes
tmp_3_4598975_A_C	346	M>L	No	1000Genomes
ENSVATH02141867	365	I>V	No	1000Genomes
tmp_3_4599935_A_G	499	N>D	No	1000Genomes
tmp_3_4600100_C_T	526	A>V	No	1000Genomes
tmp_3_4600138_C_T	539	L>F	No	1000Genomes

Type	Name	Position	InterPro Accession
domain	Biotin/lipoyl attachment	111 - 187	IPR000089
domain	2-oxoacid dehydrogenase acyltransferase, catalytic domain	309 - 539	IPR001078
binding_site	2-oxo acid dehydrogenase, lipoyl-binding site	136 - 165	IPR003016
domain	Peripheral subunit-binding domain	247 - 285	IPR004167

		Description
EC Number	2.3.1.12	Transferring groups other than amino-acyl groups
Subcellular Localization	Mitochondrion matrix
PANTHER Family
PANTHER Subfamily
PANTHER Protein Class
PANTHER Pathway Category	No pathway information available

Name	Definition
chloroplast envelope	The double lipid bilayer enclosing the chloroplast and separating its contents from the rest of the cytoplasm; includes the intermembrane space.
cytoplasm	The contents of a cell excluding the plasma membrane and nucleus, but including other subcellular structures.
cytosol	The part of the cytoplasm that does not contain organelles but which does contain other particulate matter, such as protein complexes.
mitochondrial matrix	The gel-like material, with considerable fine structure, that lies in the matrix space, or lumen, of a mitochondrion. It contains the enzymes of the tricarboxylic acid cycle and, in some organisms, the enzymes concerned with fatty acid oxidation.
mitochondrion	A semiautonomous, self replicating organelle that occurs in varying numbers, shapes, and sizes in the cytoplasm of virtually all eukaryotic cells. It is notably the site of tissue respiration.
pyruvate dehydrogenase complex	Complex that carries out the oxidative decarboxylation of pyruvate to form acetyl-CoA; comprises subunits possessing three catalytic activities: pyruvate dehydrogenase (E1), dihydrolipoamide S-acetyltransferase (E2), and dihydrolipoamide dehydrogenase (E3).

Name	Definition
acetyltransferase activity	Catalysis of the transfer of an acetyl group to an acceptor molecule.
copper ion binding	Binding to a copper (Cu) ion.
dihydrolipoyllysine-residue acetyltransferase activity	Catalysis of the reaction: acetyl-CoA + dihydrolipoamide = CoA + S-acetyldihydrolipoamide.
lipoic acid binding	Binding to lipoic acid, 1,2-dithiolane-3-pentanoic acid.

Name	Definition
glycolytic process	The chemical reactions and pathways resulting in the breakdown of a carbohydrate into pyruvate, with the concomitant production of a small amount of ATP and the reduction of NAD(P) to NAD(P)H. Glycolysis begins with the metabolism of a carbohydrate to generate products that can enter the pathway and ends with the production of pyruvate. Pyruvate may be converted to acetyl-coenzyme A, ethanol, lactate, or other small molecules.

UniProt AC	Gene Name	Protein Name	Species	Evidence Code
P11181	DBT	Lipoamide acyltransferase component of branched-chain alpha-keto acid dehydrogenase complex, mitochondrial	Bos taurus (Bovine)	PR
P11182	DBT	Lipoamide acyltransferase component of branched-chain alpha-keto acid dehydrogenase complex, mitochondrial	Homo sapiens (Human)	PR
P53395	Dbt	Lipoamide acyltransferase component of branched-chain alpha-keto acid dehydrogenase complex, mitochondrial	Mus musculus (Mouse)	PR
Q23571	dbt-1	Lipoamide acyltransferase component of branched-chain alpha-keto acid dehydrogenase complex, mitochondrial	Caenorhabditis elegans	PR
Q5M729	At1g54220	Dihydrolipoyllysine-residue acetyltransferase component 3 of pyruvate dehydrogenase complex, mitochondrial	Arabidopsis thaliana (Mouse-ear cress)	PR
Q9M7Z1	BCE2	Lipoamide acyltransferase component of branched-chain alpha-keto acid dehydrogenase complex, mitochondrial	Arabidopsis thaliana (Mouse-ear cress)	PR