AiPD: Autoinhibited Protein Database

Q8IWE4

Gene name	DCUN1D3
Protein name	DCN1-like protein 3
Names	DCNL3, DCUN1 domain-containing protein 3, Defective in cullin neddylation protein 1-like protein 3, Squamous cell carcinoma-related oncogene 3
Species	Homo sapiens (Human)
KEGG Pathway	hsa:123879
EC number
Protein Class

Descriptions

The autoinhibited protein was predicted that may have potential autoinhibitory elements via cis-regPred.

Autoinhibitory domains (AIDs)

1-117 (Predicted disordered autoinhibitory region) PR

Target domain
Relief mechanism
Assay	cis-regPred

AID

1-117 (Predicted disordered autoinhibitory region)

Target domain

Relief mechanism

Assay

cis-regPred

Accessory elements

No accessory elements

References

Yeon JH et al. (2016) "Systems-wide Identification of cis-Regulatory Elements in Proteins", Cell Systems, 2, 89-100

Autoinhibited structure

Activated structure

2 structures for Q8IWE4

Entry ID	Method	Resolution	Chain	Position	Source
4GBA	X-ray	240 A	A/B	86-304	PDB
AF-Q8IWE4-F1	Predicted				AlphaFoldDB

184 variants for Q8IWE4

Variant ID(s)	Position	Change	Description	Diseaes Association	Provenance
TCGA novel	1	M>?	Variant assessed as Somatic; impact. [NCI-TCGA]	No	NCI-TCGA
VAR_072689	2	G>S	a cancer; unknown pathological significance [UniProt]	No	UniProt
CA395015854 rs1261700256	3	Q>R		No	ClinGen gnomAD
CA7945332 rs760147404	5	V>I		No	ClinGen ExAC gnomAD
CA395015832 rs1340653940	6	T>I		No	ClinGen gnomAD
CA395015819 rs1225245805	8	C>Y		No	ClinGen gnomAD
rs1385099458 CA395015811	9	K>R		No	ClinGen gnomAD
CA395015783 rs774666903	13	S>L	Variant assessed as Somatic; 0.0 impact. [NCI-TCGA]	No	ClinGen ExAC NCI-TCGA gnomAD
rs774666903 CA7945331	13	S>W		No	ClinGen ExAC gnomAD
CA7945329 rs763189938	15	L>V		No	ClinGen ExAC gnomAD
TCGA novel	19	N>K	Variant assessed as Somatic; impact. [NCI-TCGA]	No	NCI-TCGA
rs770179050 CA7945327	22	R>C		No	ClinGen ExAC TOPMed gnomAD
CA7945326 rs549170536	22	R>H		No	ClinGen 1000Genomes ExAC gnomAD
rs1355144583 CA395015720	23	E>G		No	ClinGen TOPMed
CA395015714 rs1386584391	24	P>S		No	ClinGen gnomAD
rs1163787576 COSM968181 CA395015678	29	H>Y	Variant assessed as Somatic; 0.0 impact. endometrium [NCI-TCGA, Cosmic]	No	ClinGen cosmic curated NCI-TCGA gnomAD
rs1443602562 CA395015671	30	S>G		No	ClinGen gnomAD
rs1184418335 CA395015654	32	R>K		No	ClinGen gnomAD
CA7945324 rs7187522	32	R>W		No	ClinGen 1000Genomes ESP ExAC TOPMed gnomAD
CA395015650 rs1458173506	33	G>R		No	ClinGen TOPMed
rs1177907045 CA395015646	33	G>V		No	ClinGen TOPMed
CA395015641 rs1188578747	34	A>G		No	ClinGen TOPMed
rs369289640 CA7945321	34	A>T		No	ClinGen ESP ExAC TOPMed gnomAD
TCGA novel	34	A>V	Variant assessed as Somatic; impact. [NCI-TCGA]	No	NCI-TCGA
rs1361627621 CA395015630	36	H>R		No	ClinGen gnomAD
CA7945319 rs374769393	37	R>C		No	ClinGen ESP ExAC TOPMed gnomAD
CA395015624 rs1245399298 COSM1203177	37	R>H	large_intestine [Cosmic]	No	ClinGen cosmic curated gnomAD
CA7945315 rs754383342	41	V>A		No	ClinGen ExAC TOPMed gnomAD
rs754383342 CA395015595	41	V>E		No	ClinGen ExAC TOPMed gnomAD
CA7945316 rs767028379	41	V>L		No	ClinGen ExAC gnomAD
CA7945314 rs752142562	42	P>L		No	ClinGen ExAC gnomAD
rs766750785 CA7945313	43	P>L		No	ClinGen ExAC gnomAD
CA7945312 rs763483895	44	C>R		No	ClinGen ExAC gnomAD
CA7945311 rs371819424	47	P>L		No	ClinGen 1000Genomes ESP ExAC TOPMed gnomAD
rs371819424 CA7945310	47	P>Q		No	ClinGen 1000Genomes ESP ExAC TOPMed gnomAD
rs149292226 CA7945308	50	D>N		No	ClinGen ESP ExAC TOPMed gnomAD
CA395015542 rs1341340122	50	D>V		No	ClinGen gnomAD
rs201398772 CA7945306	51	I>T		No	ClinGen ESP ExAC TOPMed gnomAD
COSM968180 CA7945304 rs770815934	53	V>I	lung Variant assessed as Somatic; 0.0 impact. endometrium [Cosmic, NCI-TCGA]	No	ClinGen cosmic curated ExAC NCI-TCGA TOPMed gnomAD
CA7945302 rs777603187	55	G>R		No	ClinGen ExAC gnomAD
CA395015501 rs1270309613	57	K>Q		No	ClinGen gnomAD
rs755845816 CA7945301	59	A>S		No	ClinGen ExAC gnomAD
rs1596627979 CA395015479	60	E>G		No	ClinGen Ensembl
CA7945299 rs150849224	60	E>K		No	ClinGen ESP ExAC TOPMed gnomAD
rs1596627978 CA395015475	61	A>P		No	ClinGen Ensembl
CA395015446 rs1224566273	65	A>D		No	ClinGen gnomAD
CA395015444 rs1224566273	65	A>V		No	ClinGen gnomAD
CA395015415 rs754541715	70	T>A		No	ClinGen ExAC TOPMed gnomAD
rs200201954 CA7945297	70	T>M		No	ClinGen 1000Genomes ESP ExAC TOPMed gnomAD
CA7945298 rs754541715	70	T>S		No	ClinGen ExAC TOPMed gnomAD
rs758883322 CA7945295	71	S>F		No	ClinGen ExAC TOPMed gnomAD
rs750898376 CA7945294	72	S>L		No	ClinGen ExAC TOPMed gnomAD
CA395015395 rs1347633456	74	D>H		No	ClinGen gnomAD
rs1031088486 CA279341199	77	R>G		No	ClinGen TOPMed
CA395015353 rs1308959527	80	K>R		No	ClinGen TOPMed gnomAD
rs1168345295 CA395015344	81	S>F	Variant assessed as Somatic; impact. [NCI-TCGA]	No	ClinGen NCI-TCGA TOPMed
rs764271471 CA7945290	84	E>K		No	ClinGen ExAC TOPMed gnomAD
rs760918785 CA395015314	85	E>D		No	ClinGen ExAC TOPMed gnomAD
rs1430526649 CA395015300	88	L>V		No	ClinGen gnomAD
rs775501439 CA7945288	89	Q>K		No	ClinGen ExAC
rs772180031 CA7945287	89	Q>P		No	ClinGen ExAC TOPMed gnomAD
CA7945286 rs762892022	90	R>I		No	ClinGen ExAC gnomAD
CA7945285 rs138789031	90	R>S		No	ClinGen 1000Genomes ESP ExAC TOPMed gnomAD
TCGA novel	90	R>T	Variant assessed as Somatic; impact. [NCI-TCGA]	No	NCI-TCGA
rs1379654649 CA395015262	93	E>D		No	ClinGen TOPMed gnomAD
rs747893830 CA7945283	96	R>G		No	ClinGen ExAC gnomAD
CA7945282 rs145000131	96	R>M		No	ClinGen ESP ExAC TOPMed gnomAD
CA7945281 rs768236347	97	R>C		No	ClinGen ExAC TOPMed gnomAD
CA7945280 rs368022474	97	R>H		No	ClinGen ESP ExAC TOPMed gnomAD
CA279341099 rs938518202	98	Y>C		No	ClinGen TOPMed
rs529534017 CA7945279	99	K>R		No	ClinGen 1000Genomes ExAC gnomAD
rs141394610 CA7945277	102	R>Q		No	ClinGen ESP ExAC TOPMed gnomAD
rs373669655 CA7945278	102	R>W		No	ClinGen ESP ExAC TOPMed gnomAD
TCGA novel	105	A>T	Variant assessed as Somatic; impact. [NCI-TCGA]	No	NCI-TCGA
TCGA novel	108	E>K	Variant assessed as Somatic; impact. [NCI-TCGA]	No	NCI-TCGA
rs1318128909 CA395015121	111	M>L		No	ClinGen gnomAD
rs757815463 CA7945275	112	E>A		No	ClinGen ExAC gnomAD
TCGA novel	112	E>V	Variant assessed as Somatic; impact. [NCI-TCGA]	No	NCI-TCGA
COSM182803 CA279341066 rs200217368	113	R>C	large_intestine [Cosmic]	No	ClinGen cosmic curated gnomAD
rs200217368 CA395015090	113	R>G		No	ClinGen gnomAD
rs754293777 CA7945274	113	R>H		No	ClinGen ExAC TOPMed gnomAD
CA7945273 rs764516441	114	F>I		No	ClinGen ExAC gnomAD
CA7945272 rs760866653	116	N>S		No	ClinGen ExAC TOPMed gnomAD
CA7945271 rs752906928	119	C>S		No	ClinGen ExAC gnomAD
CA395014969 rs1427292595	121	D>E		No	ClinGen TOPMed
CA395014974 rs1475167245	121	D>G		No	ClinGen gnomAD
TCGA novel	121	D>N	Variant assessed as Somatic; impact. [NCI-TCGA]	No	NCI-TCGA
rs759576387 CA395014902	126	R>P		No	ClinGen ExAC gnomAD
CA7945269 rs759576387	126	R>Q		No	ClinGen ExAC gnomAD
rs1243493536 CA395014897	127	V>M		No	ClinGen gnomAD
rs776344865 CA7945265	131	A>V		No	ClinGen ExAC gnomAD
rs768438103 CA395014816	133	K>R		No	ClinGen ExAC TOPMed gnomAD
CA7945264 rs768438103	133	K>T		No	ClinGen ExAC TOPMed gnomAD
rs1340860611 CA395014770	136	A>P		No	ClinGen gnomAD
CA395014761 rs746642001	137	A>S		No	ClinGen ExAC TOPMed gnomAD
CA7945263 rs746642001	137	A>T		No	ClinGen ExAC TOPMed gnomAD
rs779713076 CA7945262	138	T>A		No	ClinGen ExAC gnomAD
CA7945261 rs771728742	138	T>S		No	ClinGen ExAC gnomAD
CA7945260 rs745419737	141	K>R		No	ClinGen ExAC TOPMed gnomAD
rs1299200520 CA395013859	146	E>K		No	ClinGen gnomAD
rs77432343 CA279340104	148	F>L		No	ClinGen Ensembl
rs1453586218 CA395013813	149	D>N		No	ClinGen TOPMed
rs776561494 CA7945247	153	A>T		No	ClinGen ExAC gnomAD
CA395013742 rs1425453128	153	A>V		No	ClinGen gnomAD
CA7945246 rs368597287	155	S>G		No	ClinGen ESP ExAC gnomAD
CA7945245 rs199765506	155	S>R		No	ClinGen ESP ExAC TOPMed gnomAD
rs1177612698 CA395013645	160	D>G		No	ClinGen gnomAD
rs202156477 CA7945241	161	G>R	Variant assessed as Somatic; 0.0 impact. [NCI-TCGA]	No	ClinGen 1000Genomes ExAC NCI-TCGA gnomAD
CA395013623 rs1158122023	162	I>N		No	ClinGen TOPMed
rs770510257 CA7945240	163	C>S	Variant assessed as Somatic; 0.0 impact. [NCI-TCGA]	No	ClinGen ExAC NCI-TCGA TOPMed gnomAD
TCGA novel	163	C>W	Variant assessed as Somatic; impact. [NCI-TCGA]	No	NCI-TCGA
rs770510257 CA395013611	163	C>Y		No	ClinGen ExAC TOPMed gnomAD
rs143835944 CA395013586	165	R>L		No	ClinGen ESP ExAC gnomAD
CA7945239 rs143835944	165	R>Q		No	ClinGen ESP ExAC gnomAD
CA279340069 rs199941022	167	P>S		No	ClinGen 1000Genomes
rs374366221 CA7945237	168	S>R		No	ClinGen ESP ExAC TOPMed gnomAD
CA7945236 rs748599193	171	T>A		No	ClinGen ExAC gnomAD
TCGA novel	173	A>P	Variant assessed as Somatic; impact. [NCI-TCGA]	No	NCI-TCGA
rs755262727 CA7945234	173	A>V		No	ClinGen ExAC TOPMed gnomAD
rs766449301 CA7945233	174	K>R		No	ClinGen ExAC TOPMed gnomAD
CA7945232 rs766449301	174	K>T		No	ClinGen ExAC TOPMed gnomAD
CA395013454 rs1405570212	175	Q>R		No	ClinGen gnomAD
TCGA novel	179	F>V	Variant assessed as Somatic; impact. [NCI-TCGA]	No	NCI-TCGA
rs758460882 CA7945230	181	D>A		No	ClinGen ExAC gnomAD
rs1346065603 CA395013373	181	D>E		No	ClinGen gnomAD
CA279340004 rs940141790	181	D>Y		No	ClinGen TOPMed
CA7945229 rs753737940	184	R>Q		No	ClinGen ExAC gnomAD
rs764140413 CA7945228	186	T>A		No	ClinGen ExAC gnomAD
rs775421022 CA7945226	192	D>Y		No	ClinGen ExAC gnomAD
TCGA novel	197	Q>*	Variant assessed as Somatic; impact. [NCI-TCGA]	No	NCI-TCGA
CA7945225 rs767406827	197	Q>K		No	ClinGen ExAC gnomAD
rs1339285105 CA395013149	198	R>Q	Variant assessed as Somatic; impact. [NCI-TCGA]	No	ClinGen NCI-TCGA gnomAD
CA279339967 rs909974055	198	R>W		No	ClinGen TOPMed gnomAD
CA395013076 rs1265490547	204	I>T		No	ClinGen gnomAD
CA7945224 rs558043046	204	I>V		No	ClinGen 1000Genomes ExAC gnomAD
CA279339942 rs148978111	211	L>P		No	ClinGen ESP
rs145837764 CA7945222	215	Q>H		No	ClinGen ESP ExAC TOPMed gnomAD
TCGA novel	216	N>Q	Variant assessed as Somatic; impact. [NCI-TCGA]	No	NCI-TCGA
rs1384055824 CA395012892	217	N>S		No	ClinGen TOPMed gnomAD
CA7945221 rs748783398	219	P>L		No	ClinGen ExAC TOPMed gnomAD
rs748783398 CA395012865	219	P>Q		No	ClinGen ExAC TOPMed gnomAD
CA395012857 rs1456457634	220	V>I		No	ClinGen TOPMed gnomAD
TCGA novel	223	Q>R	Variant assessed as Somatic; impact. [NCI-TCGA]	No	NCI-TCGA
CA7945219 rs770377087	225	L>V		No	ClinGen ExAC gnomAD
rs149680095 CA7945218	229	T>I	Variant assessed as Somatic; 0.0 impact. [NCI-TCGA]	No	ClinGen ESP ExAC NCI-TCGA TOPMed gnomAD
CA7945217 rs781598413	233	S>L		No	ClinGen ExAC TOPMed gnomAD
rs1429777844 CA395012616 CA395012617	236	K>N		No	ClinGen TOPMed gnomAD
rs1165661912 CA395012626	236	K>Q		No	ClinGen TOPMed
rs1318578612 CA395012621	236	K>R		No	ClinGen TOPMed gnomAD
rs780197306 CA7945214	237	G>R		No	ClinGen ExAC gnomAD
VAR_072690	239	S>F	a cancer; unknown pathological significance [UniProt]	No	UniProt
CA279339888 rs921234573	240	R>W		No	ClinGen TOPMed
TCGA novel	242	T>S	Variant assessed as Somatic; impact. [NCI-TCGA]	No	NCI-TCGA
CA395012486 rs1463793529	245	M>I		No	ClinGen TOPMed gnomAD
rs750556211 CA7945212	248	N>K		No	ClinGen ExAC gnomAD
CA395012432 rs1204768239	249	F>L		No	ClinGen gnomAD
rs539392630 CA279339877	258	S>R		No	ClinGen 1000Genomes
CA395012147 rs1229622091	267	P>L		No	ClinGen gnomAD
CA395012123 rs1297084435	269	L>H		No	ClinGen gnomAD
CA7945210 rs756132549	269	L>I	Variant assessed as Somatic; 0.0 impact. [NCI-TCGA]	No	ClinGen ExAC NCI-TCGA gnomAD
rs752625678 CA7945209	270	F>S		No	ClinGen ExAC
rs201159661 CA279339848	271	D>G		No	ClinGen 1000Genomes
CA395012082 rs1234624249	272	T>S		No	ClinGen TOPMed
CA7945207 rs767425680	274	V>M		No	ClinGen ExAC TOPMed gnomAD
rs911752536 COSM3936962 CA279339839	280	R>*	oesophagus [Cosmic]	No	ClinGen cosmic curated TOPMed gnomAD
CA279339838 rs142074017	280	R>Q	Variant assessed as Somatic; 0.0 impact. [NCI-TCGA]	No	ClinGen ESP NCI-TCGA gnomAD
rs1252928312 CA395011954	281	R>G		No	ClinGen TOPMed
CA395011921 rs1339819017	283	R>K		No	ClinGen TOPMed
TCGA novel	284	E>K	Variant assessed as Somatic; impact. [NCI-TCGA]	No	NCI-TCGA
rs1170755803 CA395011888	285	G>W		No	ClinGen gnomAD
TCGA novel	286	E>G	Variant assessed as Somatic; impact. [NCI-TCGA]	No	NCI-TCGA
CA7945205 rs751280783	287	G>E		No	ClinGen ExAC TOPMed gnomAD
CA395011859 rs1249895641	287	G>R		No	ClinGen TOPMed
TCGA novel	288	R>E	Variant assessed as Somatic; impact. [NCI-TCGA]	No	NCI-TCGA
rs372534815 CA279339832	291	L>F		No	ClinGen ESP TOPMed gnomAD
CA395011772 rs1173145534	293	S>L	Variant assessed as Somatic; impact. [NCI-TCGA]	No	ClinGen NCI-TCGA TOPMed
CA395011764 rs1366434232	294	G>E		No	ClinGen TOPMed gnomAD
CA7945202 rs772847778	295	P>L		No	ClinGen ExAC gnomAD
CA279339811 rs1022048304	296	E>A		No	ClinGen TOPMed gnomAD
CA395011726 rs1442214298	297	G>V		No	ClinGen gnomAD
rs1167051721 CA395011712	298	L>F		No	ClinGen TOPMed
rs777080283 CA7945199	301	E>K		No	ClinGen ExAC gnomAD
CA395011629 rs1442887626	304	T>A		No	ClinGen gnomAD
rs62033352 CA279339792	304	T>I		No	ClinGen Ensembl

No associated diseases with Q8IWE4

1 regional properties for Q8IWE4

Type	Name	Position	InterPro Accession
domain	Potentiating neddylation domain	86 - 278	IPR005176

Functions

		Description
EC Number
Subcellular Localization	Cell membrane Cytoplasm Nucleus Cytoplasm, perinuclear region	After UVC treatment, the protein enters to the nucleus gradually (PubMed:18823379) Cell membrane localization is essential for CUL3 neddylation (PubMed:19617556)
PANTHER Family
PANTHER Subfamily
PANTHER Protein Class
PANTHER Pathway Category	No pathway information available

5 GO annotations of cellular component

Name	Definition
cytoplasm	The contents of a cell excluding the plasma membrane and nucleus, but including other subcellular structures.
nucleus	A membrane-bounded organelle of eukaryotic cells in which chromosomes are housed and replicated. In most cells, the nucleus contains all of the cell's chromosomes except the organellar chromosomes, and is the site of RNA synthesis and processing. In some species, or in specialized cell types, RNA metabolism or DNA replication may be absent.
perinuclear region of cytoplasm	Cytoplasm situated near, or occurring around, the nucleus.
plasma membrane	The membrane surrounding a cell that separates the cell from its external environment. It consists of a phospholipid bilayer and associated proteins.
ubiquitin ligase complex	A protein complex that includes a ubiquitin-protein ligase and enables ubiquitin protein ligase activity. The complex also contains other proteins that may confer substrate specificity on the complex.

3 GO annotations of molecular function

Name	Definition
cullin family protein binding	Binding to a member of the cullin family, hydrophobic proteins that act as scaffolds for ubiquitin ligases (E3).
ubiquitin conjugating enzyme binding	Binding to a ubiquitin conjugating enzyme, any of the E2 proteins.
ubiquitin-like protein binding	Binding to a small conjugating protein such as ubiquitin or a ubiquitin-like protein.

11 GO annotations of biological process

Name	Definition
negative regulation of cell growth	Any process that stops, prevents, or reduces the frequency, rate, extent or direction of cell growth.
negative regulation of G1/S transition of mitotic cell cycle	Any signalling pathway that decreases or inhibits the activity of a cell cycle cyclin-dependent protein kinase to modulate the switch from G1 phase to S phase of the mitotic cell cycle.
negative regulation of protein neddylation	Any process that stops, prevents or reduces the frequency, rate or extent of protein neddylation.
positive regulation of apoptotic process	Any process that activates or increases the frequency, rate or extent of cell death by apoptotic process.
positive regulation of protein neddylation	Any process that activates or increases the frequency, rate or extent of protein neddylation.
positive regulation of ubiquitin-protein transferase activity	Any process that activates, maintains or increases the rate of ubiquitin transferase activity.
protein neddylation	Covalent attachment of the ubiquitin-like protein NEDD8 (RUB1) to another protein.
regulation of cell cycle process	Any process that modulates a cellular process that is involved in the progression of biochemical and morphological phases and events that occur in a cell during successive cell replication or nuclear replication events.
regulation of protein neddylation	Any process that modulates the frequency, rate or extent of protein neddylation.
response to gamma radiation	Any process that results in a change in state or activity of a cell or an organism (in terms of movement, secretion, enzyme production, gene expression, etc.) as a result of a gamma radiation stimulus. Gamma radiation is a form of electromagnetic radiation (EMR) or light emission of a specific frequency produced from sub-atomic particle interaction, such as electron-positron annihilation and radioactive decay. Gamma rays are generally characterized as EMR having the highest frequency and energy, and also the shortest wavelength, within the electromagnetic radiation spectrum.
response to UV-C	Any process that results in a change in state or activity of a cell or an organism (in terms of movement, secretion, enzyme production, gene expression, etc.) as a result of a UV-C radiation stimulus. UV-C radiation (UV-C light) spans the wavelengths 100 to 280 nm.

5 homologous proteins in AiPD

UniProt AC	Gene Name	Protein Name	Species	Evidence Code
Q5E9V1	DCUN1D3	DCN1-like protein 3	Bos taurus (Bovine)	PR
Q8K0V2	Dcun1d3	DCN1-like protein 3	Mus musculus (Mouse)	PR
Q4V8B2	Dcun1d3	DCN1-like protein 3	Rattus norvegicus (Rat)	PR
Q9U3C8	dcn-1	Defective in cullin neddylation protein 1	Caenorhabditis elegans	PR
A4IHK8	dcun1d3	DCN1-like protein 3	Xenopus tropicalis (Western clawed frog) (Silurana tropicalis)	PR

10	20	30	40	50	60
MGQCVTKCKN	PSSTLGSKNG	DREPSNKSHS	RRGAGHREEQ	VPPCGKPGGD	ILVNGTKKAE
70	80	90	100	110	120
AATEACQLPT	SSGDAGRESK	SNAEESSLQR	LEELFRRYKD	EREDAILEEG	MERFCNDLCV
130	140	150	160	170	180
DPTEFRVLLL	AWKFQAATMC	KFTRKEFFDG	CKAISADSID	GICARFPSLL	TEAKQEDKFK
190	200	210	220	230	240
DLYRFTFQFG	LDSEEGQRSL	HREIAIALWK	LVFTQNNPPV	LDQWLNFLTE	NPSGIKGISR
250	260	270	280	290	300
DTWNMFLNFT	QVIGPDLSNY	SEDEAWPSLF	DTFVEWEMER	RKREGEGRGA	LSSGPEGLCP

EEQT