AiPD: Autoinhibited Protein Database

Q86VN1

Gene name	VPS36 (C13orf9, EAP45, CGI-145)
Protein name	Vacuolar protein-sorting-associated protein 36
Names	ELL-associated protein of 45 kDa, ESCRT-II complex subunit VPS36
Species	Homo sapiens (Human)
KEGG Pathway	hsa:51028
EC number
Protein Class

Descriptions

The autoinhibited protein was predicted that may have potential autoinhibitory elements via cis-regPred.

Autoinhibitory domains (AIDs)

46-241 (Predicted disordered autoinhibitory region) PR

Target domain
Relief mechanism
Assay	cis-regPred

AID

46-241 (Predicted disordered autoinhibitory region)

Target domain

Relief mechanism

Assay

cis-regPred

Accessory elements

No accessory elements

References

Yeon JH et al. (2016) "Systems-wide Identification of cis-Regulatory Elements in Proteins", Cell Systems, 2, 89-100

Autoinhibited structure

Activated structure

4 structures for Q86VN1

Entry ID	Method	Resolution	Chain	Position	Source
2HTH	X-ray	270 A	B	1-138	PDB
2ZME	X-ray	290 A	B	149-386	PDB
3CUQ	X-ray	261 A	B	169-386	PDB
AF-Q86VN1-F1	Predicted				AlphaFoldDB

237 variants for Q86VN1

Variant ID(s)	Position	Change	Description	Diseaes Association	Provenance
rs1263446947 CA388047106	2	D>G		No	ClinGen gnomAD
CA6992677 rs577147750	3	R>C		No	ClinGen 1000Genomes ExAC gnomAD
CA6992676 rs577147750	3	R>G		No	ClinGen 1000Genomes ExAC gnomAD
CA6992675 rs761266589	6	W>R		No	ClinGen ExAC gnomAD
rs1273724103 CA388047072	7	T>S		No	ClinGen gnomAD
CA6992674 rs773815112	8	S>N	Variant assessed as Somatic; 0.0 impact. [NCI-TCGA]	No	ClinGen ExAC NCI-TCGA gnomAD
CA6992672 rs373489248	10	L>F		No	ClinGen ESP ExAC TOPMed gnomAD
CA6992670 rs769324003	11	L>Q		No	ClinGen ExAC gnomAD
CA6992669 rs745439453	14	N>S		No	ClinGen ExAC gnomAD
CA6992668 rs780687417	16	T>S		No	ClinGen ExAC gnomAD
CA250077887 rs986422035	18	V>E		No	ClinGen TOPMed
rs953628242 CA250077882	19	I>F		No	ClinGen TOPMed
TCGA novel	19	I>T	Variant assessed as Somatic; impact. [NCI-TCGA]	No	NCI-TCGA
rs11545440 CA250077881	20	Q>K		No	ClinGen Ensembl
CA388046972 rs1167635526	21	Q>L		No	ClinGen gnomAD
CA388046956 rs1364480857	22	R>L		No	ClinGen TOPMed
rs913841674 CA250077864	22	R>S		No	ClinGen Ensembl
CA388046954 rs1391579919 CA388046953	23	G>R		No	ClinGen TOPMed gnomAD
rs1053525221 CA250077861	23	G>V		No	ClinGen Ensembl
rs1566093053 CA388046944	24	V>M		No	ClinGen Ensembl
CA388046925 rs1566093047	25	R>P		No	ClinGen Ensembl
rs1566093047 CA388046927	25	R>Q		No	ClinGen Ensembl
CA388046912 rs1453233117	26	I>S		No	ClinGen gnomAD
CA388046897 rs1195410170	27	Y>C		No	ClinGen gnomAD
rs1249733810 CA388046865	29	G>D	Variant assessed as Somatic; 0.0 impact. [NCI-TCGA]	No	ClinGen NCI-TCGA gnomAD
CA388046874 rs1435945921	29	G>S		No	ClinGen gnomAD
CA6992663 rs752432200	30	E>D		No	ClinGen ExAC TOPMed gnomAD
CA388046862 rs1248178739	30	E>K		No	ClinGen TOPMed
CA6992662 rs568175027	31	E>Q		No	ClinGen 1000Genomes ExAC gnomAD
TCGA novel	31	E>missing	Variant assessed as Somatic; impact. [NCI-TCGA]	No	NCI-TCGA
CA388046828 rs1161272051	32	K>R		No	ClinGen gnomAD
CA6992633 rs763285959	33	I>M		No	ClinGen ExAC gnomAD
CA6992634 rs764392240	33	I>T		No	ClinGen ExAC gnomAD
rs764636096 CA250072993	35	F>V		No	ClinGen Ensembl
CA388046448 rs1594123821	36	D>V		No	ClinGen Ensembl
CA388046428 rs1238247404	39	T>I		No	ClinGen gnomAD
TCGA novel	40	L>F	Variant assessed as Somatic; impact. [NCI-TCGA]	No	NCI-TCGA
rs1465760486 CA388046421	41	L>F		No	ClinGen TOPMed
rs763216059	42	L>missing	Variant assessed as Somatic; 4.625e-05 impact. [NCI-TCGA]	No	NCI-TCGA
CA250072973 rs949189663	44	T>I		No	ClinGen Ensembl
rs772613576 CA6992629	45	H>Q		No	ClinGen ExAC gnomAD
rs1056739271 CA250072953	46	R>*		No	ClinGen TOPMed
CA6992628 rs567352617	46	R>Q		No	ClinGen 1000Genomes ExAC gnomAD
CA388046377 rs1233000698	48	I>T		No	ClinGen gnomAD
CA388046344 rs1356552755	52	Q>H		No	ClinGen gnomAD
CA388046328 rs1236090178	54	N>K		No	ClinGen TOPMed
rs1211602507 CA388046304	56	E>G		No	ClinGen TOPMed
rs1325133741 CA388046306	56	E>Q		No	ClinGen gnomAD
CA388046274 rs1594122071	60	A>S		No	ClinGen Ensembl
CA6992609 rs760326692	61	I>V		No	ClinGen ExAC gnomAD
rs1167973976 CA388046257	62	L>P		No	ClinGen gnomAD
rs1268936974 CA388046254	63	L>F	Variant assessed as Somatic; impact. [NCI-TCGA]	No	ClinGen NCI-TCGA TOPMed
rs1409185275 CA388046248	64	S>A		No	ClinGen gnomAD
rs1419009084 CA388046228	67	V>M		No	ClinGen gnomAD
CA6992607 rs771502892	69	I>T		No	ClinGen ExAC TOPMed gnomAD
rs1028369384 CA250068439	69	I>V		No	ClinGen gnomAD
CA388046204 rs1566088985	70	E>D		No	ClinGen Ensembl
CA388046210 rs1193104384	70	E>Q		No	ClinGen gnomAD
rs564047614 CA6992606	71	E>K		No	ClinGen 1000Genomes ExAC gnomAD
rs768575745 CA388046191	72	Q>L		No	ClinGen gnomAD
rs768575745 CA250068431	72	Q>R		No	ClinGen gnomAD
CA388046185 rs185573531	73	A>E		No	ClinGen 1000Genomes ExAC TOPMed gnomAD
rs185573531 CA6992605	73	A>V		No	ClinGen 1000Genomes ExAC TOPMed gnomAD
CA6992603 rs749149596	74	A>V		No	ClinGen ExAC gnomAD
TCGA novel	75	G>E	Variant assessed as Somatic; impact. [NCI-TCGA]	No	NCI-TCGA
CA6992602 rs201611503	75	G>R		No	ClinGen ESP ExAC TOPMed gnomAD
CA388046164 rs1173441150	77	G>W		No	ClinGen TOPMed
CA6992582 rs762986715	80	A>T		No	ClinGen ExAC TOPMed gnomAD
rs1037480527 CA388046124	81	K>I		No	ClinGen TOPMed gnomAD
rs1037480527 CA250066979	81	K>R		No	ClinGen TOPMed gnomAD
CA388046108 rs1413345451	84	V>F		No	ClinGen gnomAD
rs1184734095 CA388046098	85	H>R		No	ClinGen gnomAD
rs1256335544 CA388046078	88	P>L		No	ClinGen TOPMed gnomAD
CA388046077 rs1256335544	88	P>Q		No	ClinGen TOPMed gnomAD
rs865991502 CA250066974	88	P>S		No	ClinGen Ensembl
CA388046056 rs1483942214	92	N>D		No	ClinGen gnomAD
CA250066963 rs926534991	92	N>K		No	ClinGen Ensembl
CA250066960 rs940562696	95	P>H		No	ClinGen TOPMed
rs776789902 CA6992577	99	Q>R		No	ClinGen ExAC TOPMed gnomAD
rs371106659 CA6992576	100	S>C		No	ClinGen ESP ExAC TOPMed gnomAD
TCGA novel	101	S>C	Variant assessed as Somatic; impact. [NCI-TCGA]	No	NCI-TCGA
CA388045986 rs747142932	102	K>*		No	ClinGen ExAC gnomAD
rs747142932 CA6992575	102	K>E		No	ClinGen ExAC gnomAD
rs758434923 CA6992573	104	S>A		No	ClinGen ExAC gnomAD
rs748675244 CA6992572	105	Y>C		No	ClinGen ExAC gnomAD
rs779368783 CA6992571	106	I>T		No	ClinGen ExAC gnomAD
CA388045943 rs1381632972 COSM1232380	108	L>P	large_intestine [Cosmic]	No	ClinGen cosmic curated gnomAD
CA388045936 rs1324803332	109	S>C		No	ClinGen TOPMed
rs755336970 CA6992570	111	K>R		No	ClinGen ExAC gnomAD
rs767129941 CA6992568	113	H>R		No	ClinGen ExAC TOPMed gnomAD
CA6992569 rs377668244	113	H>Y		No	ClinGen ESP ExAC gnomAD
CA6992566 rs751102773	114	G>C		No	ClinGen ExAC
CA6992540 rs751307637	118	F>V		No	ClinGen ExAC gnomAD
rs752241758 CA388045842	121	R>C	Variant assessed as Somatic; 0.0 impact. [NCI-TCGA]	No	ClinGen ExAC NCI-TCGA TOPMed gnomAD
rs140039960 CA6992536	121	R>H		No	ClinGen ESP ExAC TOPMed gnomAD
CA6992537 rs752241758	121	R>S		No	ClinGen ExAC TOPMed gnomAD
CA388045838 rs1373077735	122	L>V		No	ClinGen gnomAD
rs759526314 CA6992535	126	M>V		No	ClinGen ExAC gnomAD
TCGA novel	128	Q>*	Variant assessed as Somatic; impact. [NCI-TCGA]	No	NCI-TCGA
CA388045779 rs1444501222	130	R>K		No	ClinGen TOPMed gnomAD
rs778764463 CA6992533	131	W>R		No	ClinGen ExAC TOPMed gnomAD
CA388045757 rs1388857874	133	N>D		No	ClinGen gnomAD
rs1271665869 CA388045746	134	M>T		No	ClinGen TOPMed
rs1429931246 CA388045723	138	Q>K		No	ClinGen gnomAD
rs150589000 CA6992531	138	Q>P		No	ClinGen ESP ExAC TOPMed gnomAD
rs772273059 CA6992530	141	Q>K		No	ClinGen ExAC gnomAD
rs1378110757 CA388045686	143	N>S		No	ClinGen gnomAD
CA388045667 rs1417073429	146	P>T		No	ClinGen gnomAD
rs1203707334 CA388045642	148	P>S		No	ClinGen TOPMed
rs767241247 CA6992508	150	R>G		No	ClinGen ExAC gnomAD
CA6992507 rs762027049	151	I>L		No	ClinGen ExAC gnomAD
rs933736917 CA388045617	152	R>K		No	ClinGen TOPMed gnomAD
rs933736917 CA250065809	152	R>M		No	ClinGen TOPMed gnomAD
CA388045608 rs1340708870	154	V>I		No	ClinGen gnomAD
rs1277140565 CA388045595	156	I>V		No	ClinGen TOPMed
CA388045587 rs1221058057	157	V>L		No	ClinGen TOPMed
CA388045555 rs764254757	162	K>E		No	ClinGen ExAC gnomAD
CA6992505 rs764254757	162	K>Q		No	ClinGen ExAC gnomAD
rs762913176 CA6992504	167	R>G		No	ClinGen ExAC TOPMed gnomAD
rs777713470	167	R>K	Variant assessed as Somatic; 0.0 impact. [NCI-TCGA]	No	NCI-TCGA
CA6992502 rs531572536	168	K>R		No	ClinGen 1000Genomes ExAC TOPMed gnomAD
rs144817587 COSM108425 CA250065800	169	E>K	skin [Cosmic]	No	ClinGen cosmic curated Ensembl
CA388045494 rs1162446592	170	T>I		No	ClinGen gnomAD
CA6992501 rs201878072	172	K>R		No	ClinGen 1000Genomes ExAC gnomAD
rs1189648968 CA388045471	173	N>K		No	ClinGen gnomAD
CA388045475 rs1238301507	173	N>T		No	ClinGen gnomAD
CA388045196 rs1221269957	177	A>S		No	ClinGen gnomAD
CA6992477 rs774117351	181	L>F		No	ClinGen ExAC gnomAD
rs889215243 CA250064000	183	K>N		No	ClinGen TOPMed gnomAD
rs1178981124 CA388045130	183	K>Q		No	ClinGen TOPMed
CA388045096 CA6992475 rs748909695	185	M>I		No	ClinGen ExAC gnomAD
rs1341038841 CA388045105	185	M>L		No	ClinGen TOPMed gnomAD
rs1341038841 CA388045107	185	M>V		No	ClinGen TOPMed gnomAD
CA6992474 rs779434828	186	I>F		No	ClinGen ExAC gnomAD
CA388044985 rs1369651793	192	V>M		No	ClinGen TOPMed gnomAD
CA6992459 rs773458066	195	S>A		No	ClinGen ExAC TOPMed gnomAD
rs1409019088 CA388044914	198	I>V		No	ClinGen gnomAD
rs772410580 CA6992458	204	D>H	Variant assessed as Somatic; 0.0 impact. [NCI-TCGA]	No	ClinGen ExAC NCI-TCGA gnomAD
CA250063961 rs976576823	205	K>Q		No	ClinGen TOPMed
CA388044800 rs1431704845	207	G>D		No	ClinGen gnomAD
TCGA novel	211	E>K	Variant assessed as Somatic; impact. [NCI-TCGA]	No	NCI-TCGA
rs563700906 CA250063956	212	D>E		No	ClinGen Ensembl
rs1427906865 CA388044738	212	D>G		No	ClinGen gnomAD
rs1415478424 CA388044745	212	D>Y		No	ClinGen gnomAD
rs769341330 CA6992454	213	E>K		No	ClinGen ExAC gnomAD
rs1228902577 CA388044646	214	T>N		No	ClinGen TOPMed
rs1012947506 CA250063664	217	F>I		No	ClinGen TOPMed
rs1246112588 CA388044596	218	K>N		No	ClinGen gnomAD
CA388044589 rs1208096691	219	S>Y		No	ClinGen gnomAD
rs759133031 CA6992432	222	L>P		No	ClinGen ExAC TOPMed gnomAD
rs759133031 CA6992431	222	L>Q		No	ClinGen ExAC TOPMed gnomAD
rs894811249 CA250063661	222	L>V		No	ClinGen Ensembl
rs1269718136 CA388044551	223	S>C		No	ClinGen gnomAD
CA388044467 rs1256158607	230	V>A		No	ClinGen TOPMed
rs1480115640 CA388044454	232	R>G		No	ClinGen TOPMed
rs1320092151 CA388044447	232	R>K		No	ClinGen gnomAD
CA388044435 rs1193528513	233	E>G		No	ClinGen TOPMed
CA6992429 rs770436208	235	Y>C		No	ClinGen ExAC TOPMed gnomAD
CA6992426 rs771805336	236	G>R		No	ClinGen ExAC TOPMed gnomAD
CA6992427 rs771805336	236	G>S		No	ClinGen ExAC TOPMed gnomAD
rs747955028 COSM948087 CA6992425	239	T>A	Variant assessed as Somatic; 0.0 impact. endometrium [NCI-TCGA, Cosmic]	No	ClinGen cosmic curated ExAC NCI-TCGA TOPMed gnomAD
rs1393005925 CA388044371	239	T>I		No	ClinGen gnomAD
rs1390024952 CA388044362	240	Q>L		No	ClinGen TOPMed gnomAD
rs1390024952 CA388044363	240	Q>R		No	ClinGen TOPMed gnomAD
CA6992424 rs779332765	241	Y>H		No	ClinGen ExAC
rs1023958760 CA250063632	242	H>R		No	ClinGen TOPMed
CA250063629 rs148021124	243	M>I		No	ClinGen ESP
CA6992421 rs780307997	246	A>G		No	ClinGen ExAC gnomAD
rs750941087 CA6992419	247	K>I		No	ClinGen ExAC gnomAD
rs1387727498 CA388044252	250	A>T		No	ClinGen gnomAD
rs768035586 CA6992418	252	I>M		No	ClinGen ExAC gnomAD
rs1490889314 CA388043585	260	R>Q		No	ClinGen TOPMed gnomAD
CA250062989 rs937872451	261	G>A		No	ClinGen TOPMed
rs979318014 CA250062985	263	I>M		No	ClinGen TOPMed
rs144558806 CA6992394	263	I>V		No	ClinGen 1000Genomes ESP ExAC TOPMed gnomAD
rs758744494 CA6992393	265	S>P		No	ClinGen ExAC gnomAD
CA6992391 rs138713397	267	T>M		No	ClinGen ESP ExAC TOPMed gnomAD
rs867642870 CA388043493	268	E>D		No	ClinGen TOPMed gnomAD
rs766868664 CA6992388	275	R>*		No	ClinGen ExAC TOPMed
rs1330972941 COSM297920 CA388043404	277	R>*	large_intestine Variant assessed as Somatic; impact. [Cosmic, NCI-TCGA]	No	ClinGen cosmic curated NCI-TCGA gnomAD
CA6992387 rs761869362	277	R>Q		No	ClinGen ExAC TOPMed gnomAD
CA388043380 rs1348220905	279	M>I		No	ClinGen gnomAD
CA388042667 rs753095007	282	L>I		No	ClinGen ExAC gnomAD
rs753095007 CA6992372	282	L>V		No	ClinGen ExAC gnomAD
rs1303208194 CA388042656	283	S>P		No	ClinGen TOPMed
CA388042643 rs1372427100	284	P>S		No	ClinGen TOPMed
CA388042638 rs1337164881	285	E>Q		No	ClinGen gnomAD
CA388042551 rs1331574053	289	N>K		No	ClinGen gnomAD
rs779092607 CA6992371 COSM300129	290	A>V	large_intestine [Cosmic]	No	ClinGen cosmic curated ExAC TOPMed gnomAD
rs750000143 CA6992369	293	M>I		No	ClinGen ExAC gnomAD
rs767072520 CA6992368	295	E>A		No	ClinGen ExAC TOPMed gnomAD
CA388042442 rs1411030861	296	A>V		No	ClinGen gnomAD
CA388042435 rs1325852589	297	L>Q		No	ClinGen TOPMed gnomAD
rs761384772 CA6992367	298	K>T		No	ClinGen ExAC gnomAD
rs957856347 CA250061297	300	P>R		No	ClinGen gnomAD
CA388042367 rs1234837327	301	L>P		No	ClinGen gnomAD
CA388042245 rs1240260591	303	L>F		No	ClinGen gnomAD
rs778313209 CA6992357	304	R>C		No	ClinGen ExAC gnomAD
CA6992356 rs145896285	304	R>H		No	ClinGen ESP ExAC TOPMed gnomAD
rs145896285 CA388042230	304	R>P		No	ClinGen ESP ExAC TOPMed gnomAD
CA388042222 rs1449138233	305	V>L		No	ClinGen gnomAD
CA6992354 rs779363980	310	V>I		No	ClinGen ExAC gnomAD
CA6992353 rs755244819	311	M>T		No	ClinGen ExAC TOPMed gnomAD
rs750136185 CA6992352	313	I>V		No	ClinGen ExAC TOPMed gnomAD
rs756894284 CA6992350	316	Q>P		No	ClinGen ExAC TOPMed gnomAD
rs943817245 CA250061108	317	S>F		No	ClinGen Ensembl
rs1298293742 CA388042001	318	H>Y		No	ClinGen TOPMed gnomAD
rs912383022 CA250061106	319	K>E		No	ClinGen TOPMed
rs1040812952 CA250061104	319	K>R		No	ClinGen TOPMed
rs868577151 CA250061102	321	E>G		No	ClinGen Ensembl
rs763638585 CA6992348	322	E>G		No	ClinGen ExAC gnomAD
rs763017466 COSM130171 CA6992347	326	S>L	upper_aerodigestive_tract Variant assessed as Somatic; 0.0 impact. [Cosmic, NCI-TCGA]	No	ClinGen cosmic curated ExAC NCI-TCGA TOPMed gnomAD
TCGA novel	327	A>T	Variant assessed as Somatic; impact. [NCI-TCGA]	No	NCI-TCGA
rs1367291453 CA388041002	329	E>K		No	ClinGen TOPMed
CA6992345 rs765128734	330	T>A		No	ClinGen ExAC TOPMed gnomAD
CA388040771 rs1270589967	331	V>L		No	ClinGen gnomAD
rs1179647843 CA388040691	334	K>E		No	ClinGen gnomAD
rs757826427 CA6992328	336	S>T		No	ClinGen ExAC gnomAD
CA250060895 rs369735041	343	A>V		No	ClinGen ESP
CA6992324 rs753651402	345	L>P		No	ClinGen ExAC gnomAD
CA6992323 rs766099499	348	M>I		No	ClinGen ExAC gnomAD
rs1360048118 CA388040359	350	V>A		No	ClinGen gnomAD
rs1594110507 CA388040227	356	R>T		No	ClinGen Ensembl
rs574568412 CA250060877	358	L>V		No	ClinGen 1000Genomes ExAC gnomAD
CA388040054 rs1295141591	359	L>R		No	ClinGen TOPMed
rs747600868 CA6992301	365	H>R		No	ClinGen ExAC TOPMed gnomAD
rs1227765659 CA388039913	366	L>F		No	ClinGen TOPMed
rs778431031 CA6992300	368	R>C	Variant assessed as Somatic; 0.0 impact. [NCI-TCGA]	No	ClinGen ExAC NCI-TCGA TOPMed gnomAD
rs368445598 CA6992299	368	R>H		No	ClinGen ESP ExAC TOPMed gnomAD
CA388039794 rs1453931504	371	S>*		No	ClinGen gnomAD
CA6992296 rs374745105	376	R>C		No	ClinGen ESP ExAC gnomAD
CA388039699 rs750681798 COSM3376574	376	R>H	pancreas [Cosmic]	No	ClinGen cosmic curated ExAC TOPMed gnomAD
rs750681798 CA6992295	376	R>L		No	ClinGen ExAC TOPMed gnomAD
rs767628596 CA6992294	378	Y>C		No	ClinGen ExAC gnomAD
TCGA novel	378	Y>T	Variant assessed as Somatic; impact. [NCI-TCGA]	No	NCI-TCGA
rs762009281 CA6992293	379	P>S		No	ClinGen ExAC gnomAD
rs942861593 CA250060876	387	S>L		No	ClinGen Ensembl

No associated diseases with Q86VN1

1 regional properties for Q86VN1

Type	Name	Position	InterPro Accession
domain	Vacuolar protein sorting protein 36, GLUE domain	1 - 138	IPR021648

Functions

		Description
EC Number
Subcellular Localization	Cytoplasm Endosome Late endosome Membrane Nucleus	Colocalizes with ubiquitinated proteins on late endosomes Recruited to the endosome membrane to participate in vesicle formation
PANTHER Family
PANTHER Subfamily
PANTHER Protein Class
PANTHER Pathway Category	No pathway information available

7 GO annotations of cellular component

Name	Definition
cytosol	The part of the cytoplasm that does not contain organelles but which does contain other particulate matter, such as protein complexes.
endosome	A vacuole to which materials ingested by endocytosis are delivered.
ESCRT II complex	An endosomal sorting complex required for transport and functions downstream of ESCRT I complex. It consists of the class E vacuolar protein sorting (Vps) proteins and is required for the membrane recruitment of ESCRT III complex and binds to ubiquitinated cargoes.
extracellular exosome	A vesicle that is released into the extracellular region by fusion of the limiting endosomal membrane of a multivesicular body with the plasma membrane. Extracellular exosomes, also simply called exosomes, have a diameter of about 40-100 nm.
late endosome membrane	The lipid bilayer surrounding a late endosome.
lysosome	A small lytic vacuole that has cell cycle-independent morphology found in most animal cells and that contains a variety of hydrolases, most of which have their maximal activities in the pH range 5-6. The contained enzymes display latency if properly isolated. About 40 different lysosomal hydrolases are known and lysosomes have a great variety of morphologies and functions.
nucleus	A membrane-bounded organelle of eukaryotic cells in which chromosomes are housed and replicated. In most cells, the nucleus contains all of the cell's chromosomes except the organellar chromosomes, and is the site of RNA synthesis and processing. In some species, or in specialized cell types, RNA metabolism or DNA replication may be absent.

3 GO annotations of molecular function

Name	Definition
phosphatidylinositol-3-phosphate binding	Binding to phosphatidylinositol-3-phosphate, a derivative of phosphatidylinositol in which the inositol ring is phosphorylated at the 3' position.
protein C-terminus binding	Binding to a protein C-terminus, the end of a peptide chain at which the 1-carboxyl function of a constituent amino acid is not attached in peptide linkage to another amino-acid residue.
ubiquitin binding	Binding to ubiquitin, a protein that when covalently bound to other cellular proteins marks them for proteolytic degradation.

4 GO annotations of biological process

Name	Definition
macroautophagy	The major inducible pathway for the general turnover of cytoplasmic constituents in eukaryotic cells, it is also responsible for the degradation of active cytoplasmic enzymes and organelles during nutrient starvation. Macroautophagy involves the formation of double-membrane-bounded autophagosomes which enclose the cytoplasmic constituent targeted for degradation in a membrane-bounded structure. Autophagosomes then fuse with a lysosome (or vacuole) releasing single-membrane-bounded autophagic bodies that are then degraded within the lysosome (or vacuole). Some types of macroautophagy, e.g. pexophagy, mitophagy, involve selective targeting of the targets to be degraded.
membrane fission	A process that is carried out at the cellular level which results in the separation of a single continuous membrane into two membranes.
multivesicular body assembly	The aggregation, arrangement and bonding together of a set of components to form a multivesicular body, a type of late endosome in which regions of the limiting endosomal membrane invaginate to form internal vesicles; membrane proteins that enter the internal vesicles are sequestered from the cytoplasm.
protein transport to vacuole involved in ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway	The process of directing proteins towards the vacuole that contributes to protein catabolism via the multivesicular body (MVB) pathway.

3 homologous proteins in AiPD

UniProt AC	Gene Name	Protein Name	Species	Evidence Code
A5PK00	VPS36	Vacuolar protein-sorting-associated protein 36	Bos taurus (Bovine)	PR
Q9VU87	Vps36	Vacuolar protein-sorting-associated protein 36	Drosophila melanogaster (Fruit fly)	PR
Q7ZVK4	vps36	Vacuolar protein-sorting-associated protein 36	Danio rerio (Zebrafish) (Brachydanio rerio)	PR

10	20	30	40	50	60
MDRFVWTSGL	LEINETLVIQ	QRGVRIYDGE	EKIKFDAGTL	LLSTHRLIWR	DQKNHECCMA
70	80	90	100	110	120
ILLSQIVFIE	EQAAGIGKSA	KIVVHLHPAP	PNKEPGPFQS	SKNSYIKLSF	KEHGQIEFYR
130	140	150	160	170	180
RLSEEMTQRR	WENMPVSQSL	QTNRGPQPGR	IRAVGIVGIE	RKLEEKRKET	DKNISEAFED
190	200	210	220	230	240
LSKLMIKAKE	MVELSKSIAN	KIKDKQGDIT	EDETIRFKSY	LLSMGIANPV	TRETYGSGTQ
250	260	270	280	290	300
YHMQLAKQLA	GILQVPLEER	GGIMSLTEVY	CLVNRARGME	LLSPEDLVNA	CKMLEALKLP
310	320	330	340	350	360
LRLRVFDSGV	MVIELQSHKE	EEMVASALET	VSEKGSLTSE	EFAKLVGMSV	LLAKERLLLA
370	380
EKMGHLCRDD	SVEGLRFYPN	LFMTQS