Q61187

PR
Gene name
Tsg101
Protein name
Tumor susceptibility gene 101 protein
Names
ESCRT-I complex subunit TSG101
Species
Mus musculus (Mouse)
KEGG Pathway
mmu:22088
EC number
Protein Class

Descriptions

The autoinhibited protein was predicted that may have potential autoinhibitory elements via cis-regPred.

Autoinhibitory domains (AIDs)

116-343 (Predicted disordered autoinhibitory region) PR

Target domain

Relief mechanism

Assay

cis-regPred

Accessory elements

No accessory elements

References

Autoinhibited structure

Activated structure

1 structures for Q61187

Entry ID Method Resolution Chain Position Source
AF-Q61187-F1 Predicted AlphaFoldDB

28 variants for Q61187

Variant ID(s) Position Change Description Diseaes Association Provenance
rs3388911936 16 K>Q No EVA
rs3388899784 17 Y>H No EVA
rs3388911916 43 V>D No EVA
rs3388895345 58 T>R No EVA
rs3397807058 62 R>S No EVA
rs3397463741 68 Y>* No EVA
rs3388911997 81 P>L No EVA
rs3388886417 85 P>T No EVA
rs3398029796 174 A>G No EVA
rs3398029254 177 S>A No EVA
rs3398029301 177 S>Y No EVA
rs3388904412 221 T>I No EVA
rs3397847748 231 L>P No EVA
rs3397101133 231 L>V No EVA
rs3388916040 234 A>T No EVA
rs3397727382 236 S>T No EVA
rs3388904458 288 N>Y No EVA
rs3388899709 292 L>V No EVA
rs3398029322 293 K>Q No EVA
rs3388909892 303 L>M No EVA
rs3388911932 306 M>V No EVA
rs3388895250 309 Q>* No EVA
rs3388912289 311 E>D No EVA
rs3388904465 337 N>I No EVA
rs3388912414 344 F>L No EVA
rs3388915113 347 G>R No EVA
rs3388871572 350 L>* No EVA
rs3388899743 370 K>E No EVA

No associated diseases with Q61187

2 regional properties for Q61187

Type Name Position InterPro Accession
domain Ubiquitin E2 variant, N-terminal 2 - 145 IPR008883
domain Steadiness box (SB) domain 317 - 391 IPR017916

Functions

Description
EC Number
Subcellular Localization
  • Cytoplasm
  • Early endosome membrane ; Peripheral membrane protein ; Cytoplasmic side
  • Late endosome membrane ; Peripheral membrane protein
  • Cytoplasm, cytoskeleton, microtubule organizing center, centrosome
  • Midbody, Midbody ring
  • Nucleus
  • Mainly cytoplasmic
  • Membrane-associated when active and soluble when inactive
  • Nuclear localization is cell cycle-dependent
  • Interaction with CEP55 is required for localization to the midbody during cytokinesis
PANTHER Family
PANTHER Subfamily
PANTHER Protein Class
PANTHER Pathway Category No pathway information available

15 GO annotations of cellular component

Name Definition
cytoplasm The contents of a cell excluding the plasma membrane and nucleus, but including other subcellular structures.
cytosol The part of the cytoplasm that does not contain organelles but which does contain other particulate matter, such as protein complexes.
early endosome A membrane-bounded organelle that receives incoming material from primary endocytic vesicles that have been generated by clathrin-dependent and clathrin-independent endocytosis; vesicles fuse with the early endosome to deliver cargo for sorting into recycling or degradation pathways.
early endosome membrane The lipid bilayer surrounding an early endosome.
endosome A vacuole to which materials ingested by endocytosis are delivered.
endosome membrane The lipid bilayer surrounding an endosome.
ESCRT I complex An endosomal sorting complex required for transport. It consists of the class E vacuolar protein sorting (Vps) proteins and interacts with ubiquitinated cargoes.
extracellular exosome A vesicle that is released into the extracellular region by fusion of the limiting endosomal membrane of a multivesicular body with the plasma membrane. Extracellular exosomes, also simply called exosomes, have a diameter of about 40-100 nm.
Flemming body A cell part that is the central region of the midbody characterized by a gap in alpha-tubulin staining. It is a dense structure of antiparallel microtubules from the central spindle in the middle of the intercellular bridge.
late endosome A prelysosomal endocytic organelle differentiated from early endosomes by lower lumenal pH and different protein composition. Late endosomes are more spherical than early endosomes and are mostly juxtanuclear, being concentrated near the microtubule organizing center.
late endosome membrane The lipid bilayer surrounding a late endosome.
microtubule organizing center An intracellular structure that can catalyze gamma-tubulin-dependent microtubule nucleation and that can anchor microtubules by interacting with their minus ends, plus ends or sides.
nucleolus A small, dense body one or more of which are present in the nucleus of eukaryotic cells. It is rich in RNA and protein, is not bounded by a limiting membrane, and is not seen during mitosis. Its prime function is the transcription of the nucleolar DNA into 45S ribosomal-precursor RNA, the processing of this RNA into 5.8S, 18S, and 28S components of ribosomal RNA, and the association of these components with 5S RNA and proteins synthesized outside the nucleolus. This association results in the formation of ribonucleoprotein precursors; these pass into the cytoplasm and mature into the 40S and 60S subunits of the ribosome.
nucleus A membrane-bounded organelle of eukaryotic cells in which chromosomes are housed and replicated. In most cells, the nucleus contains all of the cell's chromosomes except the organellar chromosomes, and is the site of RNA synthesis and processing. In some species, or in specialized cell types, RNA metabolism or DNA replication may be absent.
plasma membrane The membrane surrounding a cell that separates the cell from its external environment. It consists of a phospholipid bilayer and associated proteins.

8 GO annotations of molecular function

Name Definition
calcium-dependent protein binding Binding to a protein or protein complex in the presence of calcium.
nuclear receptor coactivator activity A transcription coactivator activity that activates or increases the transcription of specific gene sets via binding to a DNA-bound nuclear receptor, either on its own or as part of a complex. Coactivators often act by altering chromatin structure and modifications. For example, one class of transcription coregulators modifies chromatin structure through covalent modification of histones. A second class remodels the conformation of chromatin in an ATP-dependent fashion. A third class modulates interactions of DNA-bound DNA-binding transcription factors with other transcription coregulators. A fourth class of coactivator activity is the bridging of a DNA-binding transcription factor to the general (basal) transcription machinery. The Mediator complex, which bridges sequence-specific DNA binding transcription factors and RNA polymerase, is also a transcription coactivator.
protein homodimerization activity Binding to an identical protein to form a homodimer.
protein-containing complex binding Binding to a macromolecular complex.
transcription corepressor activity A transcription coregulator activity that represses or decreases the transcription of specific gene sets via binding to a DNA-bound DNA-binding transcription factor, either on its own or as part of a complex. Corepressors often act by altering chromatin structure and modifications. For example, one class of transcription corepressors modifies chromatin structure through covalent modification of histones. A second class remodels the conformation of chromatin in an ATP-dependent fashion. A third class modulates interactions of DNA-bound DNA-binding transcription factors with other transcription coregulators.
ubiquitin binding Binding to ubiquitin, a protein that when covalently bound to other cellular proteins marks them for proteolytic degradation.
ubiquitin protein ligase binding Binding to a ubiquitin protein ligase enzyme, any of the E3 proteins.
virion binding Binding to a virion, either by binding to components of the capsid or the viral envelope.

23 GO annotations of biological process

Name Definition
cell cycle The progression of biochemical and morphological phases and events that occur in a cell during successive cell replication or nuclear replication events. Canonically, the cell cycle comprises the replication and segregation of genetic material followed by the division of the cell, but in endocycles or syncytial cells nuclear replication or nuclear division may not be followed by cell division.
cell differentiation The process in which relatively unspecialized cells, e.g. embryonic or regenerative cells, acquire specialized structural and/or functional features that characterize the cells, tissues, or organs of the mature organism or some other relatively stable phase of the organism's life history. Differentiation includes the processes involved in commitment of a cell to a specific fate and its subsequent development to the mature state.
cell division The process resulting in division and partitioning of components of a cell to form more cells; may or may not be accompanied by the physical separation of a cell into distinct, individually membrane-bounded daughter cells.
endosome to lysosome transport The directed movement of substances from endosomes to lysosomes.
exosomal secretion The process whereby a membrane-bounded vesicle is released into the extracellular region by fusion of the limiting endosomal membrane of a multivesicular body with the plasma membrane.
extracellular transport The transport of substances that occurs outside cells.
keratinocyte differentiation The process in which a relatively unspecialized cell acquires specialized features of a keratinocyte.
negative regulation of cell population proliferation Any process that stops, prevents or reduces the rate or extent of cell proliferation.
negative regulation of epidermal growth factor receptor signaling pathway Any process that stops, prevents, or reduces the frequency, rate or extent of epidermal growth factor receptor signaling pathway activity.
negative regulation of epidermal growth factor-activated receptor activity Any process that stops, prevents, or reduces the frequency, rate or extent of EGF-activated receptor activity.
negative regulation of transcription by RNA polymerase II Any process that stops, prevents, or reduces the frequency, rate or extent of transcription mediated by RNA polymerase II.
positive regulation of exosomal secretion Any process that activates or increases the frequency, rate or extent of exosomal secretion.
positive regulation of ubiquitin-dependent endocytosis Any process that activates or increases the frequency, rate or extent of ubiquitin-dependent endocytosis.
positive regulation of viral budding via host ESCRT complex Any process that activates or increases the frequency, rate or extent of viral budding via host ESCRT complex.
protein monoubiquitination Addition of a single ubiquitin group to a protein.
protein transport The directed movement of proteins into, out of or within a cell, or between cells, by means of some agent such as a transporter or pore.
regulation of cell cycle Any process that modulates the rate or extent of progression through the cell cycle.
regulation of cell growth Any process that modulates the frequency, rate, extent or direction of cell growth.
regulation of extracellular exosome assembly Any process that modulates the frequency, rate or extent of extracellular vesicular exosome assembly.
regulation of MAP kinase activity Any process that modulates the frequency, rate or extent of MAP kinase activity.
ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway The chemical reactions and pathways resulting in the breakdown of a protein or peptide covalently tagged with ubiquitin, via the multivesicular body (MVB) sorting pathway; ubiquitin-tagged proteins are sorted into MVBs, and delivered to a lysosome/vacuole for degradation.
viral budding A viral process by which enveloped viruses acquire a host-derived membrane enriched in viral proteins to form their external envelope. The process starts when nucleocapsids, assembled or in the process of being built, induce formation of a membrane curvature in the host plasma or organelle membrane and wrap up in the forming bud. The process ends when the bud is eventually pinched off by membrane scission to release the enveloped particle into the lumenal or extracellular space.
viral release from host cell The dissemination of mature viral particles from the host cell, e.g. by cell lysis or the budding of virus particles from the cell membrane.

5 homologous proteins in AiPD

UniProt AC Gene Name Protein Name Species Evidence Code
Q99816 TSG101 Tumor susceptibility gene 101 protein Homo sapiens (Human) PR
Q6IRE4 Tsg101 Tumor susceptibility gene 101 protein Rattus norvegicus (Rat) PR
Q9FFY6 ELCL Protein ELC-like Arabidopsis thaliana (Mouse-ear cress) PR
Q66KB7 uevld Ubiquitin-conjugating enzyme E2 variant 3 Xenopus tropicalis (Western clawed frog) (Silurana tropicalis) PR
Q6DBY5 uevld Ubiquitin-conjugating enzyme E2 variant 3 Danio rerio (Zebrafish) (Brachydanio rerio) PR
10 20 30 40 50 60
MAVSESQLKK MMSKYKYRDL TVRQTVNVIA MYKDLKPVLD SYVFNDGSSR ELVNLTGTIP
70 80 90 100 110 120
VRYRGNIYNI PICLWLLDTY PYNPPICFVK PTSSMTIKTG KHVDANGKIY LPYLHDWKHP
130 140 150 160 170 180
RSELLELIQI MIVIFGEEPP VFSRPTVSAS YPPYTATGPP NTSYMPGMPS GISAYPSGYP
190 200 210 220 230 240
PNPSGYPGCP YPPAGPYPAT TSSQYPSQPP VTTVGPSRDG TISEDTIRAS LISAVSDKLR
250 260 270 280 290 300
WRMKEEMDGA QAELNALKRT EEDLKKGHQK LEEMVTRLDQ EVAEVDKNIE LLKKKDEELS
310 320 330 340 350 360
SALEKMENQS ENNDIDEVII PTAPLYKQIL NLYAEENAIE DTIFYLGEAL RRGVIDLDVF
370 380 390
LKHVRLLSRK QFQLRALMQK ARKTAGLSDL Y