P28271

PR
Gene name
Aco1 (Ireb1, Irebp)
Protein name
Cytoplasmic aconitate hydratase
Names
Aconitase, Citrate hydro-lyase, Iron regulatory protein 1, IRP1, Iron-responsive element-binding protein 1, IRE-BP 1
Species
Mus musculus (Mouse)
KEGG Pathway
mmu:11428
EC number
4.2.1.3: Hydro-lyases
Protein Class

Descriptions

The autoinhibited protein was predicted that may have potential autoinhibitory elements via cis-regPred.

Autoinhibitory domains (AIDs)

595-666 (Predicted disordered autoinhibitory region) PR

Target domain

Relief mechanism

Assay

cis-regPred

Accessory elements

No accessory elements

References

Autoinhibited structure

Activated structure

1 structures for P28271

Entry ID Method Resolution Chain Position Source
AF-P28271-F1 Predicted AlphaFoldDB

45 variants for P28271

Variant ID(s) Position Change Description Diseaes Association Provenance
rs235474752 14 A>P No EVA
rs3388675015 21 F>I No EVA
rs3394151710 33 G>D No EVA
rs3388677441 33 G>R No EVA
rs3388676323 103 A>V No EVA
rs3388677501 104 V>M No EVA
rs3388678765 164 F>L No EVA
rs3388681530 225 E>V No EVA
rs3388672049 226 A>S No EVA
rs3388671709 228 A>D No EVA
rs3388674096 231 L>P No EVA
rs3388680949 243 I>N No EVA
rs3388681550 318 A>V No EVA
rs3388680953 335 K>* No EVA
rs261217103 347 N>S No EVA
rs3388678740 350 S>F No EVA
rs3388681547 355 F>S No EVA
rs3412992073 372 G>E No EVA
rs3388657159 375 R>G No EVA
rs3388670704 377 Q>* No EVA
rs3388675087 392 C>S No EVA
rs3388666653 400 K>T No EVA
rs256994865 406 P>S No EVA
rs3388670715 407 D>Y No EVA
rs222974563 412 R>C No EVA
rs108076042 412 R>H No EVA
rs27761549 418 S>N No EVA
rs3388675076 423 T>S No EVA
rs3388677461 432 A>V No EVA
rs3388680937 449 A>T No EVA
rs243212523 467 I>V No EVA
rs3388677500 468 K>* No EVA
rs3388678105 492 S>Y No EVA
rs248641798 603 H>Y No EVA
rs27761518 607 G>R No EVA
rs3388681603 621 K>Q No EVA
rs3388678761 661 S>P No EVA
rs3388675407 692 A>E No EVA
rs3388678055 736 K>N No EVA
rs3388674123 814 I>V No EVA
rs3388666685 826 S>F No EVA
rs3388675047 828 G>E No EVA
rs3388681020 832 R>W No EVA
rs242006767 846 R>H No EVA
rs3388675033 888 A>S No EVA

No associated diseases with P28271

5 regional properties for P28271

Type Name Position InterPro Accession
domain Aconitase A/isopropylmalate dehydratase small subunit, swivel domain 693 - 818 IPR000573
domain Aconitase/3-isopropylmalate dehydratase large subunit, alpha/beta/alpha domain 63 - 564 IPR001030
binding_site Aconitase family, 4Fe-4S cluster binding site 429 - 445 IPR018136-1
binding_site Aconitase family, 4Fe-4S cluster binding site 495 - 508 IPR018136-2
domain Aconitase A, swivel domain 670 - 837 IPR044137

Functions

Description
EC Number 4.2.1.3 Hydro-lyases
Subcellular Localization
  • Cytoplasm, cytosol
PANTHER Family
PANTHER Subfamily
PANTHER Protein Class
PANTHER Pathway Category No pathway information available

6 GO annotations of cellular component

Name Definition
cytoplasm The contents of a cell excluding the plasma membrane and nucleus, but including other subcellular structures.
cytosol The part of the cytoplasm that does not contain organelles but which does contain other particulate matter, such as protein complexes.
endoplasmic reticulum The irregular network of unit membranes, visible only by electron microscopy, that occurs in the cytoplasm of many eukaryotic cells. The membranes form a complex meshwork of tubular channels, which are often expanded into slitlike cavities called cisternae. The ER takes two forms, rough (or granular), with ribosomes adhering to the outer surface, and smooth (with no ribosomes attached).
Golgi apparatus A membrane-bound cytoplasmic organelle of the endomembrane system that further processes the core oligosaccharides (e.g. N-glycans) added to proteins in the endoplasmic reticulum and packages them into membrane-bound vesicles. The Golgi apparatus operates at the intersection of the secretory, lysosomal, and endocytic pathways.
intracellular membrane-bounded organelle Organized structure of distinctive morphology and function, bounded by a single or double lipid bilayer membrane and occurring within the cell. Includes the nucleus, mitochondria, plastids, vacuoles, and vesicles. Excludes the plasma membrane.
mitochondrion A semiautonomous, self replicating organelle that occurs in varying numbers, shapes, and sizes in the cytoplasm of virtually all eukaryotic cells. It is notably the site of tissue respiration.

9 GO annotations of molecular function

Name Definition
3 iron, 4 sulfur cluster binding Binding to a 3 iron, 4 sulfur (3Fe-4S) cluster; this cluster consists of three iron atoms, with the inorganic sulfur atoms found between the irons and acting as bridging ligands. It is essentially a 4Fe-4S cluster with one iron missing.
4 iron, 4 sulfur cluster binding Binding to a 4 iron, 4 sulfur (4Fe-4S) cluster; this cluster consists of four iron atoms, with the inorganic sulfur atoms found between the irons and acting as bridging ligands.
aconitate hydratase activity Catalysis of the reaction: citrate = isocitrate. The reaction occurs in two steps: (1) citrate = cis-aconitate + H2O, (2) cis-aconitate + H2O = isocitrate. This reaction is the interconversion of citrate and isocitrate via the labile, enzyme-bound intermediate cis-aconitate. Water is removed from one part of the citrate molecule and added back to a different atom to form isocitrate.
citrate dehydratase activity Catalysis of the reaction: citrate = cis-aconitate + H2O.
iron-responsive element binding Binding to an iron-responsive element, a regulatory sequence found in the 5'- and 3'-untranslated regions of mRNAs encoding many iron-binding proteins.
iron-sulfur cluster binding Binding to an iron-sulfur cluster, a combination of iron and sulfur atoms.
metal ion binding Binding to a metal ion.
mRNA 5'-UTR binding Binding to an mRNA molecule at its 5' untranslated region.
RNA binding Binding to an RNA molecule or a portion thereof.

8 GO annotations of biological process

Name Definition
cellular iron ion homeostasis Any process involved in the maintenance of an internal steady state of iron ions at the level of a cell.
citrate metabolic process The chemical reactions and pathways involving citrate, 2-hydroxy-1,2,3-propanetricarboyxlate. Citrate is widely distributed in nature and is an important intermediate in the TCA cycle and the glyoxylate cycle.
intestinal absorption Any process in which nutrients are taken up from the contents of the intestine.
post-embryonic development The process whose specific outcome is the progression of the organism over time, from the completion of embryonic development to the mature structure. See embryonic development.
regulation of gene expression Any process that modulates the frequency, rate or extent of gene expression. Gene expression is the process in which a gene's coding sequence is converted into a mature gene product (protein or RNA).
regulation of translation Any process that modulates the frequency, rate or extent of the chemical reactions and pathways resulting in the formation of proteins by the translation of mRNA or circRNA.
response to iron(II) ion Any process that results in a change in state or activity of a cell or an organism (in terms of movement, secretion, enzyme production, gene expression, etc.) as a result of an iron(II) ion stimulus.
tricarboxylic acid cycle A nearly universal metabolic pathway in which the acetyl group of acetyl coenzyme A is effectively oxidized to two CO2 and four pairs of electrons are transferred to coenzymes. The acetyl group combines with oxaloacetate to form citrate, which undergoes successive transformations to isocitrate, 2-oxoglutarate, succinyl-CoA, succinate, fumarate, malate, and oxaloacetate again, thus completing the cycle. In eukaryotes the tricarboxylic acid is confined to the mitochondria. See also glyoxylate cycle.

3 homologous proteins in AiPD

UniProt AC Gene Name Protein Name Species Evidence Code
P21399 ACO1 Cytoplasmic aconitate hydratase Homo sapiens (Human) PR
Q23500 aco-1 Cytoplasmic aconitate hydratase Caenorhabditis elegans PR
Q94A28 ACO2 Aconitate hydratase 2, mitochondrial Arabidopsis thaliana (Mouse-ear cress) PR
10 20 30 40 50 60
MKNPFAHLAE PLDAAQPGKR FFNLNKLEDS RYGRLPFSIR VLLEAAVRNC DEFLVKKNDI
70 80 90 100 110 120
ENILNWNVMQ HKNIEVPFKP ARVILQDFTG VPAVVDFAAM RDAVKKLGGN PEKINPVCPA
130 140 150 160 170 180
DLVIDHSIQV DFNRRADSLQ KNQDLEFERN KERFEFLKWG SQAFCNMRII PPGSGIIHQV
190 200 210 220 230 240
NLEYLARVVF DQDGCYYPDS LVGTDSHTTM IDGLGVLGWG VGGIEAEAVM LGQPISMVLP
250 260 270 280 290 300
QVIGYKLMGK PHPLVTSTDI VLTITKHLRQ VGVVGKFVEF FGPGVAQLSI ADRATIANMC
310 320 330 340 350 360
PEYGATAAFF PVDEVSIAYL LQTGREEDKV KHIQKYLQAV GMFRDFNDTS QDPDFTQVVE
370 380 390 400 410 420
LDLKTVVPCC SGPKRPQDKV AVSEMKKDFE SCLGAKQGFK GFQVAPDRHN DRKTFLYSNS
430 440 450 460 470 480
EFTLAHGSVV IAAITSCTNT SNPSVMLGAG LLAKKAVEAG LSVKPYIKTS LSPGSGVVTY
490 500 510 520 530 540
YLRESGVMPY LSQLGFDVVG YGCMTCIGNS GPLPEPVVEA ITQGDLVAVG VLSGNRNFEG
550 560 570 580 590 600
RVHPNTRANY LASPPLVIAY AIAGTVRIDF EKEPLGVNAQ GRQVFLKDIW PTRDEIQAVE
610 620 630 640 650 660
RQHVIPGMFK EVYQKIETVN KSWNALAAPS EKLYAWNPKS TYIKSPPFFE SLTLDLQPPK
670 680 690 700 710 720
SIVDAYVLLN LGDSVTTDHI SPAGNIARNS PAARYLTNRG LTPREFNSYG SRRGNDAIMA
730 740 750 760 770 780
RGTFANIRLL NKFLNKQAPQ TVHLPSGETL DVFDAAERYQ QAGLPLIVLA GKEYGSGSSR
790 800 810 820 830 840
DWAAKGPFLL GIKAVLAESY ERIHRSNLVG MGVIPLEYLP GETADSLGLT GRERYTINIP
850 860 870 880
EDLKPRMTVQ IKLDTGKTFQ AVMRFDTDVE LTYFHNGGIL NYMIRKMAQ