O08581

PR
Gene name
Kcnk1
Protein name
Potassium channel subfamily K member 1
Names
Inward rectifying potassium channel protein TWIK-1
Species
Mus musculus (Mouse)
KEGG Pathway
mmu:16525
EC number
Protein Class

Descriptions

The autoinhibited protein was predicted that may have potential autoinhibitory elements via cis-regPred.

Autoinhibitory domains (AIDs)

288-336 (Predicted disordered autoinhibitory region) PR

Target domain

Relief mechanism

Assay

cis-regPred

Accessory elements

No accessory elements

References

Autoinhibited structure

Activated structure

1 structures for O08581

Entry ID Method Resolution Chain Position Source
AF-O08581-F1 Predicted AlphaFoldDB

16 variants for O08581

Variant ID(s) Position Change Description Diseaes Association Provenance
rs3388989266 3 Q>* No EVA
rs3389027159 3 Q>H No EVA
rs3399777662 71 S>T No EVA
rs3399221370 88 Y>* No EVA
rs3399986979 88 Y>H No EVA
rs3389024354 106 S>* No EVA
rs3388971498 151 A>S No EVA
rs3389027166 163 R>I No EVA
rs3389020180 180 A>G No EVA
rs3389008322 183 H>Q No EVA
rs3389021467 209 D>Y No EVA
rs32063899 290 L>Q No EVA
rs32063902 297 D>H No EVA
rs3399848379 306 E>* No EVA
rs3388971577 323 A>P No EVA
rs234409038 334 A>V No EVA

No associated diseases with O08581

5 regional properties for O08581

Type Name Position InterPro Accession
conserved_site TPP-binding enzyme, conserved site 503 - 522 IPR000399
domain Thiamine pyrophosphate enzyme, TPP-binding 466 - 621 IPR011766
domain Thiamine pyrophosphate enzyme, central domain 273 - 402 IPR012000
domain Thiamine pyrophosphate enzyme, N-terminal TPP-binding domain 80 - 243 IPR012001
domain Acetolactate synthase large subunit, TPP binding domain 444 - 636 IPR039368

Functions

Description
EC Number
Subcellular Localization
  • Cell membrane ; Multi-pass membrane protein
  • Recycling endosome
  • Apical cell membrane
  • Cytoplasmic vesicle
  • Perikaryon
  • Cell projection, dendrite
  • Cell projection
  • Synaptic cell membrane
  • The heterodimer with KCNK2 is detected at the astrocyte cell membrane (PubMed:24496152)
  • Not detected at the astrocyte cell membrane when KCNK2 is absent (PubMed:24496152)
  • Detected on neuronal cell bodies, and to a lesser degree on neuronal cell projections (PubMed:12855359, PubMed:17079103)
  • Detected on hippocampus dentate gyrus granule cell bodies and to a lesser degree on proximal dendrites (PubMed:25406588)
  • Detected at the apical cell membrane in stria vascularis in the cochlea (By similarity)
  • Detected at the apical cell membrane of vestibular dark cells situated between the crista and the utricle in the inner ear (PubMed:12855359)
  • Detected at the apical cell membrane in stria vascularis in the cochlea (PubMed:12855359)
  • Detected at the apical cell membrane in kidney proximal tubule segment S1 and in subapical compartments in segments S1, S2 and S3 (PubMed:16025300)
  • Predominantly in cytoplasmic structures in kidney distal convoluted tubules and collecting ducts (PubMed:16025300)
  • Predominantly in cytoplasmic structures in hippocampus astrocytes; only a minor proportion of the protein is present at the cell membrane (PubMed:24368895)
PANTHER Family
PANTHER Subfamily
PANTHER Protein Class
PANTHER Pathway Category No pathway information available

14 GO annotations of cellular component

Name Definition
anchoring junction A cell junction that mechanically attaches a cell (and its cytoskeleton) to neighboring cells or to the extracellular matrix.
apical plasma membrane The region of the plasma membrane located at the apical end of the cell.
brush border membrane The portion of the plasma membrane surrounding the brush border.
cytoplasm The contents of a cell excluding the plasma membrane and nucleus, but including other subcellular structures.
dendrite A neuron projection that has a short, tapering, morphology. Dendrites receive and integrate signals from other neurons or from sensory stimuli, and conduct nerve impulses towards the axon or the cell body. In most neurons, the impulse is conveyed from dendrites to axon via the cell body, but in some types of unipolar neuron, the impulse does not travel via the cell body.
endosome A vacuole to which materials ingested by endocytosis are delivered.
integral component of membrane The component of a membrane consisting of the gene products and protein complexes having at least some part of their peptide sequence embedded in the hydrophobic region of the membrane.
integral component of plasma membrane The component of the plasma membrane consisting of the gene products and protein complexes having at least some part of their peptide sequence embedded in the hydrophobic region of the membrane.
intracellular membrane-bounded organelle Organized structure of distinctive morphology and function, bounded by a single or double lipid bilayer membrane and occurring within the cell. Includes the nucleus, mitochondria, plastids, vacuoles, and vesicles. Excludes the plasma membrane.
inward rectifier potassium channel complex A protein complex which is capable of inward rectifier potassium channel activity.
perikaryon The portion of the cell soma (neuronal cell body) that excludes the nucleus.
potassium channel complex An ion channel complex through which potassium ions pass.
recycling endosome An organelle consisting of a network of tubules that functions in targeting molecules, such as receptors transporters and lipids, to the plasma membrane.
synaptic membrane A specialized area of membrane on either the presynaptic or the postsynaptic side of a synapse, the junction between a nerve fiber of one neuron and another neuron or muscle fiber or glial cell.

5 GO annotations of molecular function

Name Definition
identical protein binding Binding to an identical protein or proteins.
inward rectifier potassium channel activity Enables the transmembrane transfer of a potassium ion by an inwardly-rectifying voltage-gated channel. An inwardly rectifying current-voltage relation is one where at any given driving force the inward flow of K+ ions exceeds the outward flow for the opposite driving force. The inward-rectification is due to a voltage-dependent block of the channel pore by a specific ligand or ligands, and as a result the macroscopic conductance depends on the difference between membrane voltage and the K+ equilibrium potential rather than on membrane voltage itself.
potassium channel activity Enables the facilitated diffusion of a potassium ion (by an energy-independent process) involving passage through a transmembrane aqueous pore or channel without evidence for a carrier-mediated mechanism.
potassium ion leak channel activity Enables the transport of a potassium ion across a membrane via a narrow pore channel that is open even in an unstimulated or 'resting' state.
sodium channel activity Enables the facilitated diffusion of a sodium ion (by an energy-independent process) involving passage through a transmembrane aqueous pore or channel without evidence for a carrier-mediated mechanism.

6 GO annotations of biological process

Name Definition
potassium ion transmembrane transport A process in which a potassium ion is transported from one side of a membrane to the other.
potassium ion transport The directed movement of potassium ions (K+) into, out of or within a cell, or between cells, by means of some agent such as a transporter or pore.
regulation of resting membrane potential Any process that modulates the establishment or extent of a resting potential, the electrical charge across the plasma membrane, with the interior of the cell negative with respect to the exterior. The resting potential is the membrane potential of a cell that is not stimulated to be depolarized or hyperpolarized.
response to nicotine Any process that results in a change in state or activity of a cell or an organism (in terms of movement, secretion, enzyme production, gene expression, etc.) as a result of a nicotine stimulus.
sodium ion transmembrane transport A process in which a sodium ion is transported from one side of a membrane to the other by means of some agent such as a transporter or pore.
stabilization of membrane potential The accomplishment of a non-fluctuating membrane potential, the electric potential existing across any membrane arising from charges in the membrane itself and from the charges present in the media on either side of the membrane.

5 homologous proteins in AiPD

UniProt AC Gene Name Protein Name Species Evidence Code
Q7Z418 KCNK18 Potassium channel subfamily K member 18 Homo sapiens (Human) PR
Q96T55 KCNK16 Potassium channel subfamily K member 16 Homo sapiens (Human) PR
Q6VV64 Kcnk18 Potassium channel subfamily K member 18 Mus musculus (Mouse) PR
Q850M0 TPKA Two pore potassium channel a Oryza sativa subsp japonica (Rice) PR
Q22271 unc-58 Uncoordinated protein 58 Caenorhabditis elegans PR
10 20 30 40 50 60
MLQSLAGSSC VRLVERHRSA WCFGFLVLGY LLYLVFGAVV FSSVELPYED LLRQELRKLK
70 80 90 100 110 120
RRFLEEHECL SEPQLEQFLG RVLEASNYGV SVLSNASGNW NWDFTSALFF ASTVLSTTGY
130 140 150 160 170 180
GHTVPLSDGG KAFCIIYSVI GIPFTLLFLT AVVQRVTVHV TRRPVLYFHI RWGFSKQVVA
190 200 210 220 230 240
IVHAVLLGFV TVSCFFFIPA AVFSVLEDDW NFLESFYFCF ISLSTIGLGD YVPGEGYNQK
250 260 270 280 290 300
FRELYKIGIT CYLLLGLIAM LVVLETFCEL HELKKFRKMF YVKKDKDEDL VHIMEHDQLS
310 320 330
FSSVTEQVAG LKEEQKQSEP FVASQSPPYE DGSADH