Q8VHF0

SS
Gene name
Mark3
Protein name
MAP/microtubule affinity-regulating kinase 3
Names
EC 2.7.11.1
Species
Mus musculus (Mouse)
KEGG Pathway
rno:170577
EC number
2.7.11.1: Protein-serine/threonine kinases
Protein Class

Descriptions

Nek9 is a NIMA-related kinase essential for proper mitotic spindle assembly. A human homolog Nek7 (Q8TDX7) is subject to autoinhibition by a tyrosine motif, Tyr97, like human Nek6 (Tyr108). This is because the Y97A mutation is more active than wild-type Nek7. A similar tyrosine motif is also found in mouse Nek9.

Autoinhibitory domains (AIDs)

697-797 (KA1 domain) SS

Target domain

56-307 (Protein kinase domain)

Relief mechanism

Others

Assay

Accessory elements

195-217 (Activation loop from InterPro)

Target domain

56-307 (Protein kinase domain)

Relief mechanism

Assay

References

Autoinhibited structure

Activated structure

1 structures for Q8VHF0

Entry ID Method Resolution Chain Position Source
AF-Q8VHF0-F1 Predicted AlphaFoldDB

No variants for Q8VHF0

Variant ID(s) Position Change Description Diseaes Association Provenance
No variants for Q8VHF0

No associated diseases with Q8VHF0

9 regional properties for Q8VHF0

Type Name Position InterPro Accession
repeat Regulator of chromosome condensation, RCC1 388 - 444 IPR000408-1
repeat Regulator of chromosome condensation, RCC1 444 - 498 IPR000408-2
repeat Regulator of chromosome condensation, RCC1 499 - 550 IPR000408-3
repeat Regulator of chromosome condensation, RCC1 551 - 615 IPR000408-4
repeat Regulator of chromosome condensation, RCC1 616 - 668 IPR000408-5
repeat Regulator of chromosome condensation, RCC1 669 - 726 IPR000408-6
domain Protein kinase domain 52 - 308 IPR000719
active_site Serine/threonine-protein kinase, active site 172 - 184 IPR008271
domain Serine/threonine-protein kinase Nek9, catalytic domain 51 - 296 IPR042767

Functions

Description
EC Number 2.7.11.1 Protein-serine/threonine kinases
Subcellular Localization
  • Cytoplasm
  • Nucleus
PANTHER Family
PANTHER Subfamily
PANTHER Protein Class
PANTHER Pathway Category No pathway information available

3 GO annotations of cellular component

Name Definition
cytoplasm The contents of a cell excluding the plasma membrane and nucleus, but including other subcellular structures.
dendrite A neuron projection that has a short, tapering, morphology. Dendrites receive and integrate signals from other neurons or from sensory stimuli, and conduct nerve impulses towards the axon or the cell body. In most neurons, the impulse is conveyed from dendrites to axon via the cell body, but in some types of unipolar neuron, the impulse does not travel via the cell body.
plasma membrane The membrane surrounding a cell that separates the cell from its external environment. It consists of a phospholipid bilayer and associated proteins.

4 GO annotations of molecular function

Name Definition
ATP binding Binding to ATP, adenosine 5'-triphosphate, a universally important coenzyme and enzyme regulator.
protein serine kinase activity Catalysis of the reactions
protein serine/threonine kinase activity Catalysis of the reactions
tau-protein kinase activity Catalysis of the reaction

8 GO annotations of biological process

Name Definition
intracellular signal transduction The process in which a signal is passed on to downstream components within the cell, which become activated themselves to further propagate the signal and finally trigger a change in the function or state of the cell.
microtubule cytoskeleton organization A process that is carried out at the cellular level which results in the assembly, arrangement of constituent parts, or disassembly of cytoskeletal structures comprising microtubules and their associated proteins.
negative regulation of hippo signaling Any process that stops, prevents, or reduces the frequency, rate or extent of hippo signaling.
negative regulation of protein localization to nucleus Any process that stops, prevents or reduces the frequency, rate or extent of protein localization to nucleus.
peptidyl-serine autophosphorylation The phosphorylation by a protein of one or more of its own serine amino acid residues, or a serine residue on an identical protein.
peptidyl-serine phosphorylation The phosphorylation of peptidyl-serine to form peptidyl-O-phospho-L-serine.
positive regulation of protein binding Any process that activates or increases the frequency, rate or extent of protein binding.
protein phosphorylation The process of introducing a phosphate group on to a protein.

18 homologous proteins in AiPD

UniProt AC Gene Name Protein Name Species Evidence Code
Q96L34 MARK4 MAP/microtubule affinity-regulating kinase 4 Homo sapiens (Human) SS
P57059 SIK1 Serine/threonine-protein kinase SIK1 Homo sapiens (Human) PR
Q7KZI7 MARK2 Serine/threonine-protein kinase MARK2 Homo sapiens (Human) SS
Q9P0L2 MARK1 Serine/threonine-protein kinase MARK1 Homo sapiens (Human) EV
P27448 MARK3 MAP/microtubule affinity-regulating kinase 3 Homo sapiens (Human) SS
Q8VHJ5 Mark1 Serine/threonine-protein kinase MARK1 Mus musculus (Mouse) SS
Q8C0N0 Gm4922 Sperm motility kinase Z Mus musculus (Mouse) PR
Q8C0X8 Sperm motility kinase X Mus musculus (Mouse) PR
Q8CIP4 Mark4 MAP/microtubule affinity-regulating kinase 4 Mus musculus (Mouse) SS
A0AUV4 Gm7168 Sperm motility kinase Y Mus musculus (Mouse) PR
Q05512 Mark2 Serine/threonine-protein kinase MARK2 Mus musculus (Mouse) SS
Q03141 Mark3 MAP/microtubule affinity-regulating kinase 3 Mus musculus (Mouse) SS
O08678 Mark1 Serine/threonine-protein kinase MARK1 Rattus norvegicus (Rat) SS
O08679 Mark2 Serine/threonine-protein kinase MARK2 Rattus norvegicus (Rat) SS
Q852Q1 OSK4 Serine/threonine protein kinase OSK4 Oryza sativa subsp. japonica (Rice) SS
Q852Q2 OSK1 Serine/threonine protein kinase OSK1 Oryza sativa subsp. japonica (Rice) SS
Q852Q0 OSK3 Serine/threonine protein kinase OSK3 Oryza sativa subsp. japonica (Rice) SS
Q9TW45 par-1 Serine/threonine-protein kinase par-1 Caenorhabditis elegans SS
10 20 30 40 50 60
MSVLGEYERH CDSINSDFGS ESGGGGDSGP GPSAVPGPRA GGGAAEQEEL HYIPIRVLGR
70 80 90 100 110 120
GAFGEATLYR RTEDDSLVVW KEVDLTRLSE KERRDALNEI VILALLQHDN IIAYYNHFMD
130 140 150 160 170 180
NTTLLIELEY CNGGNLYDKI LRQKDKLFEE EMVVWYLFQI VSAVSCIHKA GILHRDIKTL
190 200 210 220 230 240
NIFLTKANLI KLGDYGLAKK LNSEYSMAET LVGTPYYMSP ELCQGVKYNF KSDIWAVGCV
250 260 270 280 290 300
IFELLTLKRT FDATNPLNLC VKIVQGIRAM EVDSSQYSLE LIQLVHACLD QDPEQRPAAD
310 320 330 340 350 360
ALLDLPLLRT RRREMEEKVT LLNAPTKRPR SSTVTEAPIA VVTSRTSEVY VWGGGKSTPQ
370 380 390 400 410 420
KLDVIKSGCS ARQVCAGNTH FAVVTVEKEL YTWVNMQGGT KLHGQLGHGD KASYRQPKHV
430 440 450 460 470 480
EKLQGKAIHQ VSCGDDFTVC VTDEGQLYAF GSDYYGCMGV DKVSGPEVLE PMQLNFFLSN
490 500 510 520 530 540
PVEQVSCGDN HVVVLTRNKE VYSWGCGEYG RLGLDSEEDY YTPQRVDVPK ALIIVAVQCG
550 560 570 580 590 600
CDGTFLLTQS GKVLACGLNE FNKLGLNQCM SGIINHEAYH EVPYTTSFTL AKQLSFYKIR
610 620 630 640 650 660
TIAPGKTHTA AIDERGRLLT FGCNKCGQLG VGNYKKRLGI NLLGGPLGGK QVIRVSCGDE
670 680 690 700 710 720
FTIAATDDNH IFAWGNGGNG RLAMTPTERP HGSDICTSWP RPIFGSLHHV PDLSCRGWHT
730 740 750 760 770 780
ILIVEKVLNS KTIRSNSSGL SIGTVVQSSS PGGGIGGGGG GGGGGGGEEE DSQQESETPD
790 800 810 820 830 840
PSGGFRGTME ADRGMEGLIS PTEAVGNSCG ASSSCPGWLR KELENAEFIP MPDSPAPLSA
850 860 870 880 890 900
AFSQSEKDTL PYEELQGLKV ASEVPPEPQR AAGAWPPRLD PAVPCVGKAL TSAACACSAL
910 920 930 940 950 960
QVEVDRLQAL VLKCLEEQQK LQQENLQMFT QLQKLNKKLE GGQQVGMHSR GTQTAKEEME
970 980
MDPKPDLDSE SWCLLGTDSC RPSL