Q8VDF2

PR
Gene name
Uhrf1 (Np95)
Protein name
E3 ubiquitin-protein ligase UHRF1
Names
Nuclear protein 95, Nuclear zinc finger protein Np95, RING-type E3 ubiquitin transferase UHRF1, Ubiquitin-like PHD and RING finger domain-containing protein 1, mUhrf1, Ubiquitin-like-containing PHD and RING finger domains protein 1
Species
Mus musculus (Mouse)
KEGG Pathway
mmu:18140
EC number
2.3.2.27: Aminoacyltransferases
Protein Class

Descriptions

The autoinhibited protein was predicted that may have potential autoinhibitory elements via cis-regPred.

Autoinhibitory domains (AIDs)

47-199 (Predicted disordered autoinhibitory region) PR

Target domain

Relief mechanism

Assay

cis-regPred

Accessory elements

No accessory elements

References

Autoinhibited structure

Activated structure

15 structures for Q8VDF2

Entry ID Method Resolution Chain Position Source
2ZKD X-ray 160 A A/B 404-613 PDB
2ZKE X-ray 260 A A 404-613 PDB
2ZKF X-ray 255 A A 404-613 PDB
2ZKG X-ray 177 A A/B/C/D 404-613 PDB
2ZO0 X-ray 219 A B 419-628 PDB
2ZO1 X-ray 196 A B 419-628 PDB
2ZO2 X-ray 309 A B 419-628 PDB
3F8I X-ray 229 A A/B 419-628 PDB
3F8J X-ray 199 A B 417-628 PDB
3FDE X-ray 141 A A/B 419-628 PDB
6M2V X-ray 300 A A/B 417-628 PDB
6VEE NMR - A 122-305 PDB
6VFO NMR - A 303-380 PDB
7XGA NMR - A 304-372 PDB
AF-Q8VDF2-F1 Predicted AlphaFoldDB

39 variants for Q8VDF2

Variant ID(s) Position Change Description Diseaes Association Provenance
rs3389463265 99 V>L No EVA
rs3389467338 126 T>R No EVA
rs3389472784 183 K>E No EVA
rs3389439806 222 N>I No EVA
rs3389439807 226 D>N No EVA
rs3389467394 231 R>H No EVA
rs3407765303 248 A>V No EVA
rs3389488778 256 R>T No EVA
rs3389421063 273 E>D No EVA
rs228748449 281 K>N No EVA
rs3389432096 295 P>L No EVA
rs3389472394 315 N>H No EVA
rs3389473666 315 N>I No EVA
rs108896295 323 C>W No EVA
rs3389432046 327 G>D No EVA
rs3389476078 408 C>* No EVA
rs3389483786 427 P>S No EVA
rs3407736701 429 V>A No EVA
rs3389383715 431 V>M No EVA
rs3411050361 432 G>D No EVA
rs3389421066 452 A>P No EVA
rs3389451912 470 G>D No EVA
rs3389483828 472 E>G No EVA
rs3389472748 492 S>F No EVA
rs3389472329 492 S>P No EVA
rs3389472748 492 S>Y No EVA
rs3407469358 501 S>P No EVA
rs3389472338 514 A>P No EVA
rs3389421053 518 H>Y No EVA
rs3389472790 615 Y>F No EVA
rs3389476034 615 Y>H No EVA
rs257069315 672 E>V No EVA
rs3389483874 691 V>M No EVA
rs3389476011 699 P>T No EVA
rs3389467314 707 V>M No EVA
rs3389473643 725 T>S No EVA
rs3389467893 727 V>M No EVA
rs108835161 753 F>Y No EVA
rs3389473617 780 S>I No EVA

No associated diseases with Q8VDF2

9 regional properties for Q8VDF2

Type Name Position InterPro Accession
domain Ubiquitin-like domain 1 - 78 IPR000626
domain Zinc finger, RING-type 323 - 369 IPR001841-1
domain Zinc finger, RING-type 713 - 752 IPR001841-2
domain Zinc finger, PHD-type 322 - 369 IPR001965
domain SRA-YDG 419 - 590 IPR003105
conserved_site Zinc finger, RING-type, conserved site 728 - 737 IPR017907
domain Zinc finger, PHD-finger 304 - 371 IPR019787
domain UHRF1, tandem tudor domain 129 - 280 IPR021991
domain E3 ubiquitin-protein ligase UHRF1, ubiquitin-like domain 1 - 74 IPR047406

Functions

Description
EC Number 2.3.2.27 Aminoacyltransferases
Subcellular Localization
  • Nucleus
  • Associated, through the YDG domain (also called SRA domain), with replicating DNA from early to late S phase, including at replicating pericentric heterochromatin (PubMed:36056023)
  • Also localizes to euchromatic regions
  • In non-S-phase cells, homogenously distributed through the nucleus (PubMed:36056023)
PANTHER Family
PANTHER Subfamily
PANTHER Protein Class
PANTHER Pathway Category No pathway information available

9 GO annotations of cellular component

Name Definition
chromatin The ordered and organized complex of DNA, protein, and sometimes RNA, that forms the chromosome.
cytoplasmic vesicle A vesicle found in the cytoplasm of a cell.
euchromatin A dispersed and relatively uncompacted form of chromatin that is in a transcription-competent conformation.
heterochromatin A compact and highly condensed form of chromatin that is refractory to transcription.
nuclear matrix The dense fibrillar network lying on the inner side of the nuclear membrane.
nucleoplasm That part of the nuclear content other than the chromosomes or the nucleolus.
nucleus A membrane-bounded organelle of eukaryotic cells in which chromosomes are housed and replicated. In most cells, the nucleus contains all of the cell's chromosomes except the organellar chromosomes, and is the site of RNA synthesis and processing. In some species, or in specialized cell types, RNA metabolism or DNA replication may be absent.
plasma membrane The membrane surrounding a cell that separates the cell from its external environment. It consists of a phospholipid bilayer and associated proteins.
replication fork The Y-shaped region of a replicating DNA molecule, resulting from the separation of the DNA strands and in which the synthesis of new strands takes place. Also includes associated protein complexes.

9 GO annotations of molecular function

Name Definition
cis-regulatory region sequence-specific DNA binding Binding to a specific upstream regulatory DNA sequence (transcription factor recognition sequence or binding site) located in cis relative to the transcription start site (i.e., on the same strand of DNA) of a gene transcribed by some RNA polymerase. The proximal promoter is in cis with and relatively close to the core promoter.
hemi-methylated DNA-binding Binding to double-stranded hemi-methylated DNA at replication foci (one strand methylated, while the other strand is unmethylated). Methylation of cytosine or adenine in DNA is an important mechanism for establishing stable heritable epigenetic marks.
histone binding Binding to a histone, any of a group of water-soluble proteins found in association with the DNA of eukaryotic or archaeal chromosomes. They are involved in the condensation and coiling of chromosomes during cell division and have also been implicated in gene regulation and DNA replication. They may be chemically modified (methylated, acetlyated and others) to regulate gene transcription.
identical protein binding Binding to an identical protein or proteins.
methyl-CpG binding Binding to a methylated cytosine/guanine dinucleotide.
methylated histone binding Binding to a histone in which a residue has been modified by methylation.
ubiquitin protein ligase activity Catalysis of the transfer of ubiquitin to a substrate protein via the reaction X-ubiquitin + S -> X + S-ubiquitin, where X is either an E2 or E3 enzyme, the X-ubiquitin linkage is a thioester bond, and the S-ubiquitin linkage is an amide bond: an isopeptide bond between the C-terminal glycine of ubiquitin and the epsilon-amino group of lysine residues in the substrate or, in the linear extension of ubiquitin chains, a peptide bond the between the C-terminal glycine and N-terminal methionine of ubiquitin residues.
ubiquitin-protein transferase activity Catalysis of the transfer of ubiquitin from one protein to another via the reaction X-Ub + Y --> Y-Ub + X, where both X-Ub and Y-Ub are covalent linkages.
zinc ion binding Binding to a zinc ion (Zn).

12 GO annotations of biological process

Name Definition
cell cycle The progression of biochemical and morphological phases and events that occur in a cell during successive cell replication or nuclear replication events. Canonically, the cell cycle comprises the replication and segregation of genetic material followed by the division of the cell, but in endocycles or syncytial cells nuclear replication or nuclear division may not be followed by cell division.
chromatin organization The assembly or remodeling of chromatin composed of DNA complexed with histones, other associated proteins, and sometimes RNA.
DNA repair The process of restoring DNA after damage. Genomes are subject to damage by chemical and physical agents in the environment (e.g. UV and ionizing radiations, chemical mutagens, fungal and bacterial toxins, etc.) and by free radicals or alkylating agents endogenously generated in metabolism. DNA is also damaged because of errors during its replication. A variety of different DNA repair pathways have been reported that include direct reversal, base excision repair, nucleotide excision repair, photoreactivation, bypass, double-strand break repair pathway, and mismatch repair pathway.
histone monoubiquitination The modification of histones by addition of a single ubiquitin group.
histone ubiquitination The modification of histones by addition of ubiquitin groups.
maintenance of DNA methylation Any process involved in maintaining the methylation state of a nucleotide sequence.
negative regulation of transcription by RNA polymerase II Any process that stops, prevents, or reduces the frequency, rate or extent of transcription mediated by RNA polymerase II.
positive regulation of protein metabolic process Any process that activates or increases the frequency, rate or extent of the chemical reactions and pathways involving a protein.
protein autoubiquitination The ubiquitination by a protein of one or more of its own amino acid residues, or residues on an identical protein. Ubiquitination occurs on the lysine residue by formation of an isopeptide crosslink.
protein ubiquitination The process in which one or more ubiquitin groups are added to a protein.
regulation of epithelial cell proliferation Any process that modulates the frequency, rate or extent of epithelial cell proliferation.
ubiquitin-dependent protein catabolic process The chemical reactions and pathways resulting in the breakdown of a protein or peptide by hydrolysis of its peptide bonds, initiated by the covalent attachment of a ubiquitin group, or multiple ubiquitin groups, to the protein.

2 homologous proteins in AiPD

UniProt AC Gene Name Protein Name Species Evidence Code
A7E320 UHRF1 E3 ubiquitin-protein ligase UHRF1 Bos taurus (Bovine) PR
Q7TPK1 Uhrf1 E3 ubiquitin-protein ligase UHRF1 Rattus norvegicus (Rat) PR
10 20 30 40 50 60
MWIQVRTMDG KETHTVNSLS RLTKVQELRK KIEEVFHVEP QLQRLFYRGK QMEDGHTLFD
70 80 90 100 110 120
YDVRLNDTIQ LLVRQSLALP LSTKERDSEL SDSDSGYGVG HSESDKSSTH GEGAAEADDK
130 140 150 160 170 180
TVWEDTDLGL YKVNEYVDVR DNIFGAWFEA QVVQVQKRAL SEDEPCSSSA VKTSEDDIMY
190 200 210 220 230 240
HVKYDDYPEH GVDIVKAKNV RARARTVIPW ENLEVGQVVM ANYNVDYPRK RGFWYDVEIC
250 260 270 280 290 300
RKRQTRTARE LYGNIRLLND SQLNNCRIMF VDEVLMIELP KERRPLIASP SQPPPALRNT
310 320 330 340 350 360
GKSGPSCRFC KDDENKPCRK CACHVCGGRE APEKQLLCDE CDMAFHLYCL KPPLTSVPPE
370 380 390 400 410 420
PEWYCPSCRT DSSEVVQAGE KLKESKKKAK MASATSSSRR DWGKGMACVG RTTECTIVPA
430 440 450 460 470 480
NHFGPIPGVP VGTMWRFRVQ VSESGVHRPH VAGIHGRSND GAYSLVLAGG YEDDVDNGNY
490 500 510 520 530 540
FTYTGSGGRD LSGNKRTAGQ SSDQKLTNNN RALALNCHSP INEKGAEAED WRQGKPVRVV
550 560 570 580 590 600
RNMKGGKHSK YAPAEGNRYD GIYKVVKYWP ERGKSGFLVW RYLLRRDDTE PEPWTREGKD
610 620 630 640 650 660
RTRQLGLTMQ YPEGYLEALA NKEKSRKRPA KALEQGPSSS KTGKSKQKST GPTLSSPRAS
670 680 690 700 710 720
KKSKLEPYTL SEQQANLIKE DKGNAKLWDD VLTSLQDGPY QIFLSKVKEA FQCICCQELV
730 740 750 760 770 780
FRPVTTVCQH NVCKDCLDRS FRAQVFSCPA CRFELDHSSP TRVNQPLQTI LNQLFPGYGS
GR