Q12246

PR
Gene name
LCB4
Protein name
Sphingoid long chain base kinase 4
Names
LCB kinase 4, Sphinganine kinase 4
Species
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
KEGG Pathway
sce:YOR171C
EC number
2.7.1.91: Phosphotransferases with an alcohol group as acceptor
Protein Class

Descriptions

The autoinhibited protein was predicted that may have potential autoinhibitory elements via cis-regPred.

Autoinhibitory domains (AIDs)

77-246 (Predicted disordered autoinhibitory region) PR

Target domain

Relief mechanism

Assay

cis-regPred

Accessory elements

No accessory elements

References

Autoinhibited structure

Activated structure

1 structures for Q12246

Entry ID Method Resolution Chain Position Source
AF-Q12246-F1 Predicted AlphaFoldDB

18 variants for Q12246

Variant ID(s) Position Change Description Diseaes Association Provenance
s15-653879 3 V>M No SGRP
s15-653800 29 H>P No SGRP
s15-653776 37 S>Y No SGRP
s15-653770 39 S>F No SGRP
s15-653761 42 S>N No SGRP
s15-653692 65 L>R No SGRP
s15-653626 87 V>A No SGRP
s15-653527 120 S>N No SGRP
s15-653492 132 Q>K No SGRP
s15-653469 139 K>N No SGRP
s15-653432 152 D>N No SGRP
s15-653384 168 Q>E No SGRP
s15-652991 299 V>I No SGRP
s15-652953 311 D>E No SGRP
s15-652865 341 Y>H No SGRP
s15-652538 450 P>S No SGRP
s15-652486 467 S>N No SGRP
s15-652444 481 N>I No SGRP

No associated diseases with Q12246

1 regional properties for Q12246

Type Name Position InterPro Accession
domain Diacylglycerol kinase, catalytic domain 224 - 368 IPR001206

Functions

Description
EC Number 2.7.1.91 Phosphotransferases with an alcohol group as acceptor
Subcellular Localization
  • Cell membrane ; Peripheral membrane protein
  • Endoplasmic reticulum membrane ; Peripheral membrane protein
  • Late endosome membrane ; Peripheral membrane protein
  • Golgi apparatus membrane ; Peripheral membrane protein
  • LCB4 is synthesized in the cytosol, palmitoylated at the Golgi apparatus by AKR1, and then transported to the plasma membrane (PM) via the late Sec pathway
  • Most of the LCB4 is delivered further to the cortical ER (cER), which is closely adjacent to the PM
  • In stationary phase, G1 cell cycle arrest causes ER-localized LCB4 to be transported to the Golgi apparatus via the early Sec pathway, and finally to the vacuole via the MVB pathway, where it is degraded
PANTHER Family
PANTHER Subfamily
PANTHER Protein Class
PANTHER Pathway Category No pathway information available

11 GO annotations of cellular component

Name Definition
cell periphery The part of a cell encompassing the cell cortex, the plasma membrane, and any external encapsulating structures.
cortical endoplasmic reticulum A cortical network of highly dynamic tubules that are juxtaposed to the plasma membrane and undergo ring closure and tubule-branching movements.
cytoplasm The contents of a cell excluding the plasma membrane and nucleus, but including other subcellular structures.
endoplasmic reticulum The irregular network of unit membranes, visible only by electron microscopy, that occurs in the cytoplasm of many eukaryotic cells. The membranes form a complex meshwork of tubular channels, which are often expanded into slitlike cavities called cisternae. The ER takes two forms, rough (or granular), with ribosomes adhering to the outer surface, and smooth (with no ribosomes attached).
endoplasmic reticulum membrane The lipid bilayer surrounding the endoplasmic reticulum.
Golgi apparatus A membrane-bound cytoplasmic organelle of the endomembrane system that further processes the core oligosaccharides (e.g. N-glycans) added to proteins in the endoplasmic reticulum and packages them into membrane-bound vesicles. The Golgi apparatus operates at the intersection of the secretory, lysosomal, and endocytic pathways.
Golgi membrane The lipid bilayer surrounding any of the compartments of the Golgi apparatus.
intracellular membrane-bounded organelle Organized structure of distinctive morphology and function, bounded by a single or double lipid bilayer membrane and occurring within the cell. Includes the nucleus, mitochondria, plastids, vacuoles, and vesicles. Excludes the plasma membrane.
late endosome membrane The lipid bilayer surrounding a late endosome.
membrane A lipid bilayer along with all the proteins and protein complexes embedded in it an attached to it.
plasma membrane The membrane surrounding a cell that separates the cell from its external environment. It consists of a phospholipid bilayer and associated proteins.

4 GO annotations of molecular function

Name Definition
ATP binding Binding to ATP, adenosine 5'-triphosphate, a universally important coenzyme and enzyme regulator.
D-erythro-sphingosine kinase activity Catalysis of the reaction: sphingosine + ATP = sphingosine 1-phosphate + ADP.
lipid kinase activity Catalysis of the phosphorylation of a simple or complex lipid.
sphinganine kinase activity Catalysis of the reaction: ATP + sphinganine = ADP + sphinganine 1-phosphate.

4 GO annotations of biological process

Name Definition
calcium-mediated signaling Any intracellular signal transduction in which the signal is passed on within the cell via calcium ions.
phosphorylation The process of introducing a phosphate group into a molecule, usually with the formation of a phosphoric ester, a phosphoric anhydride or a phosphoric amide.
sphingolipid metabolic process The chemical reactions and pathways involving sphingolipids, any of a class of lipids containing the long-chain amine diol sphingosine or a closely related base (a sphingoid).
sphingosine biosynthetic process The chemical reactions and pathways resulting in the formation of sphingosine (sphing-4-enine), trans-D-erytho-2-amino-octadec-4-ene-1,3-diol, a long chain amino diol sphingoid base that occurs in most sphingolipids in animal tissues.

2 homologous proteins in AiPD

UniProt AC Gene Name Protein Name Species Evidence Code
Q06147 LCB5 Sphingoid long chain base kinase 5 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) PR
Q8L7L1 SPHK1 Sphingosine kinase 1 Arabidopsis thaliana (Mouse-ear cress) PR
10 20 30 40 50 60
MVVQKKLRAI LTDEGVLIKS QSHHMFNKHG QLRSGDSLSL LSCLSCLDDG TLSSDGGSFD
70 80 90 100 110 120
EDDSLELLPL NTTIPFNRIL NAKYVNVGQK GFNNGKISSN PFQTENLSSS SENDDVENHS
130 140 150 160 170 180
LSNDKAPVSE SQSFPKKDKW DTKTNTVKVS PDDSQDNSPS LGIKDNQQLI ELTFAVPKGH
190 200 210 220 230 240
DVIPQKLTLL IDHVSRKSRA NTGEENISSG TVEEILEKSY ENSKRNRSIL VIINPHGGKG
250 260 270 280 290 300
TAKNLFLTKA RPILVESGCK IEIAYTKYAR HAIDIAKDLD ISKYDTIACA SGDGIPYEVI
310 320 330 340 350 360
NGLYRRPDRV DAFNKLAVTQ LPCGSGNAMS ISCHWTNNPS YAALCLVKSI ETRIDLMCCS
370 380 390 400 410 420
QPSYMNEWPR LSFLSQTYGV IAESDINTEF IRWMGPVRFN LGVAFNIIQG KKYPCEVFVK
430 440 450 460 470 480
YAAKSKKELK VHFLENKDKN KGCLTFEPNP SPNSSPDLLS KNNINNSTKD ELSPNFLNED
490 500 510 520 530 540
NFKLKYPMTE PVPRDWEKMD SELTDNLTIF YTGKMPYIAK DTKFFPAALP ADGTIDLVIT
550 560 570 580 590 600
DARIPVTRMT PILLSLDKGS HVLEPEVIHS KILAYKIIPK VESGLFSVDG EKFPLEPLQV
610 620
EIMPMLCKTL LRNGRYIDTE FESM