AiPD: Autoinhibited Protein Database

Gene name	SIZ1 (ULL1, YDR409W)
Protein name	E3 SUMO-protein ligase SIZ1
Names	E3 SUMO-protein transferase SIZ2, SAP and Miz-finger domain-containing protein 1, Ubiquitin-like protein ligase 1
Species	Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
KEGG Pathway	sce:YDR409W
EC number
Protein Class

The autoinhibited protein was predicted that may have potential autoinhibitory elements via cis-regPred.

528-607 (Predicted disordered autoinhibitory region) PR

528-607 (Predicted disordered autoinhibitory region)

cis-regPred

No accessory elements

Entry ID	Method	Resolution	Chain	Position	Source
2RNN	NMR	-	A	1-111	PDB
3I2D	X-ray	260 A	A	112-465	PDB
5JNE	X-ray	285 A	A/E	167-449	PDB
AF-Q04195-F1	Predicted				AlphaFoldDB

Variant ID(s)	Position	Change	Diseaes Association	Provenance
s04-1289482	29	E>K	No	SGRP
s04-1289806	137	R>G	No	SGRP
s04-1289877	160	N>K	No	SGRP
s04-1289948	184	K>R	No	SGRP
s04-1290065	223	N>T	No	SGRP
s04-1290391	332	Q>E	No	SGRP
s04-1290499	368	M>L	No	SGRP
s04-1290739	448	D>N	No	SGRP
s04-1290860	488	D>G	No	SGRP
s04-1290878	494	G>E	No	SGRP
s04-1290928	511	N>D	No	SGRP
s04-1291001	535	N>S	No	SGRP
s04-1291007	537	N>I	No	SGRP
s04-1291015	540	D>N	No	SGRP
s04-1291028	544	S>N	No	SGRP
s04-1291063	556	H>N	No	SGRP
s04-1291072	559	G>S	No	SGRP
s04-1291076	560	S>N	No	SGRP
s04-1291100	568	N>S	No	SGRP
s04-1291105	570	T>A	No	SGRP
s04-1291150	585	L>F	No	SGRP
s04-1291157	587	A>V	No	SGRP
s04-1291169	591	S>N	No	SGRP
s04-1291199	601	I>T	No	SGRP
s04-1291427	677	V>A	No	SGRP
s04-1291475	693	A>V	No	SGRP
s04-1291529	711	T>I	No	SGRP
s04-1291531	712	V>I	No	SGRP
s04-1291634	746	R>H	No	SGRP
s04-1291646	750	M>T	No	SGRP
s04-1291714	773	P>S	No	SGRP
s04-1291780	795	L>V	No	SGRP
s04-1291861	822	V>L	No	SGRP
s04-1291879	828	S>A	No	SGRP
s04-1292020	875	Q>K	No	SGRP

Type	Name	Position	InterPro Accession
domain	SAP domain	34 - 68	IPR003034
domain	Zinc finger, MIZ-type	344 - 431	IPR004181
domain	PINIT domain	162 - 314	IPR023321
domain	E3 SUMO-protein ligase SIZ1/2, SP-RING finger	354 - 409	IPR031141

		Description
EC Number
Subcellular Localization	Cytoplasm Nucleus Bud neck	Present at the bud neck in early M-phase
PANTHER Family
PANTHER Subfamily
PANTHER Protein Class
PANTHER Pathway Category	No pathway information available

Name	Definition
cellular bud neck	The constriction between the mother cell and daughter cell (bud) in an organism that reproduces by budding.
chromatin	The ordered and organized complex of DNA, protein, and sometimes RNA, that forms the chromosome.
nucleus	A membrane-bounded organelle of eukaryotic cells in which chromosomes are housed and replicated. In most cells, the nucleus contains all of the cell's chromosomes except the organellar chromosomes, and is the site of RNA synthesis and processing. In some species, or in specialized cell types, RNA metabolism or DNA replication may be absent.
septin ring	A tight ring-shaped structure that forms in the division plane at the site of cytokinesis; composed of members of the conserved family of filament-forming proteins called septins as well as septin-associated proteins. This type of septin structure is observed at the bud neck of budding fungal cells, at the site of cell division in animal cells, at the junction between the mother cell and a pseudohyphal projection, and also within hyphae of filamentous fungi at sites where a septum will form.

Name	Definition
SUMO transferase activity	Catalysis of the transfer of SUMO from one protein to another via the reaction X-SUMO + Y --> Y-SUMO + X, where both X-SUMO and Y-SUMO are covalent linkages.
zinc ion binding	Binding to a zinc ion (Zn).

Name	Definition
chromosome segregation	The process in which genetic material, in the form of chromosomes, is organized into specific structures and then physically separated and apportioned to two or more sets. In eukaryotes, chromosome segregation begins with the condensation of chromosomes, includes chromosome separation, and ends when chromosomes have completed movement to the spindle poles.
DNA double-strand break attachment to nuclear envelope	A process in which the DNA double-strand breaks are attached to the inner surface of the nuclear envelope proximal to the spindle pole body, or iMTOCs.
negative regulation of protein ubiquitination	Any process that stops, prevents, or reduces the frequency, rate or extent of the addition of ubiquitin groups to a protein.
protein sumoylation	The process in which a SUMO protein (small ubiquitin-related modifier) is conjugated to a target protein via an isopeptide bond between the carboxy-terminus of SUMO with an epsilon-amino group of a lysine residue of the target protein.

UniProt AC	Gene Name	Protein Name	Species	Evidence Code
Q12216	NFI1	E3 SUMO-protein ligase SIZ2	Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)	PR
Q8N2W9	PIAS4	E3 SUMO-protein ligase PIAS4	Homo sapiens (Human)	PR
O75925	PIAS1	E3 SUMO-protein ligase PIAS1	Homo sapiens (Human)	PR