Q04067
Gene name |
TIF35 |
Protein name |
Eukaryotic translation initiation factor 3 subunit G |
Names |
eIF3g, Eukaryotic translation initiation factor 3 RNA-binding subunit, eIF-3 RNA-binding subunit, Translation initiation factor eIF3 p33 subunit, eIF3 p33 |
Species |
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
KEGG Pathway |
sce:YDR429C |
EC number |
|
Protein Class |
|
Descriptions
The autoinhibited protein was predicted that may have potential autoinhibitory elements via cis-regPred.
Autoinhibitory domains (AIDs)
Target domain |
|
Relief mechanism |
|
Assay |
cis-regPred |
Accessory elements
No accessory elements
Autoinhibited structure
Activated structure
11 structures for Q04067
| Entry ID | Method | Resolution | Chain | Position | Source |
|---|---|---|---|---|---|
| 3JAP | EM | 490 A | s | 45-96 | PDB |
| 4U1E | X-ray | 200 A | G | 1-135 | PDB |
| 6FYX | EM | 305 A | r | 1-274 | PDB |
| 6FYY | EM | 305 A | r | 1-274 | PDB |
| 6GSM | EM | 515 A | r | 48-96 | PDB |
| 6GSN | EM | 575 A | r | 48-96 | PDB |
| 6ZCE | EM | 530 A | r | 1-274 | PDB |
| 6ZU9 | EM | 620 A | r | 1-135 | PDB |
| 8CAH | EM | 300 A | r | 1-274 | PDB |
| 8CAS | EM | 330 A | r | 1-274 | PDB |
| AF-Q04067-F1 | Predicted | AlphaFoldDB |
4 variants for Q04067
| Variant ID(s) | Position | Change | Description | Diseaes Association | Provenance |
|---|---|---|---|---|---|
| s04-1324878 | 139 | N>S | No | SGRP | |
| s04-1324830 | 155 | G>D | No | SGRP | |
| s04-1324824 | 157 | A>V | No | SGRP | |
| s04-1324801 | 165 | V>I | No | SGRP |
No associated diseases with Q04067
5 GO annotations of cellular component
| Name | Definition |
|---|---|
| cytoplasmic stress granule | A dense aggregation in the cytosol composed of proteins and RNAs that appear when the cell is under stress. |
| eukaryotic 43S preinitiation complex | A protein complex composed of the 40S ribosomal subunit plus eIF1A, eIF3, and eIF2-GTP-bound methionyl-initiator methionine tRNA. |
| eukaryotic 48S preinitiation complex | A protein complex composed of the small ribosomal subunit, eIF3, eIF1A, methionyl-initiatior methionine and a capped mRNA. The complex is initially positioned at the 5'-end of the capped mRNA. |
| eukaryotic translation initiation factor 3 complex | A complex of several polypeptides that plays at least two important roles in protein synthesis: First, eIF3 binds to the 40S ribosome and facilitates loading of the Met-tRNA/eIF2.GTP ternary complex to form the 43S preinitiation complex. Subsequently, eIF3 apparently assists eIF4 in recruiting mRNAs to the 43S complex. The eIF3 complex contains five conserved core subunits, and may contain several additional proteins; the non-core subunits are thought to mediate association of the complex with specific sets of mRNAs. |
| multi-eIF complex | A multifactor complex composed of multiple translation initiation factors and the initiatior tRNAiMet, which is ready to bind to the small (40S) ribosome to form the 43S preinitiation complex. In S. cerevisiae, this complex is composed of eIF1, eIF2, eIF3, and eIF5. |
2 GO annotations of molecular function
| Name | Definition |
|---|---|
| RNA binding | Binding to an RNA molecule or a portion thereof. |
| translation initiation factor activity | Functions in the initiation of ribosome-mediated translation of mRNA into a polypeptide. |
5 GO annotations of biological process
| Name | Definition |
|---|---|
| formation of cytoplasmic translation initiation complex | Joining of the large subunit, with release of IF2/eIF2 and IF3/eIF3. This leaves the functional ribosome at the AUG, with the methionyl/formyl-methionyl-tRNA positioned at the P site. |
| positive regulation of ATP-dependent activity | Any process that activates or increases the rate of an ATP-dependent activity. |
| translation reinitiation | A gene-specific translational control mechanism where the small ribosomal subunit remains attached to the mRNA following termination of translation, then resumes scanning on the same mRNA molecule and initiates again at a downstream start site. Reinitiation depends on de novo recruitment of the ternary complex that is required to recognize the next AUG codon. |
| translational initiation | The process preceding formation of the peptide bond between the first two amino acids of a protein. This includes the formation of a complex of the ribosome, mRNA or circRNA, and an initiation complex that contains the first aminoacyl-tRNA. |
| translational termination | The process resulting in the release of a polypeptide chain from the ribosome, usually in response to a termination codon (UAA, UAG, or UGA in the universal genetic code). |
7 homologous proteins in AiPD
| UniProt AC | Gene Name | Protein Name | Species | Evidence Code |
|---|---|---|---|---|
| Q9VDM6 | eIF3g2 | Eukaryotic translation initiation factor 3 subunit G-2 | Drosophila melanogaster (Fruit fly) | PR |
| Q9W4X7 | eIF3g1 | Eukaryotic translation initiation factor 3 subunit G-1 | Drosophila melanogaster (Fruit fly) | PR |
| O75821 | EIF3G | Eukaryotic translation initiation factor 3 subunit G | Homo sapiens (Human) | PR |
| Q9Z1D1 | Eif3g | Eukaryotic translation initiation factor 3 subunit G | Mus musculus (Mouse) | PR |
| Q5RK09 | Eif3g | Eukaryotic translation initiation factor 3 subunit G | Rattus norvegicus (Rat) | PR |
| Q19706 | eif-3.G | Eukaryotic translation initiation factor 3 subunit G | Caenorhabditis elegans | PR |
| Q28CY2 | eif3g | Eukaryotic translation initiation factor 3 subunit G | Xenopus tropicalis (Western clawed frog) (Silurana tropicalis) | PR |
| 10 | 20 | 30 | 40 | 50 | 60 |
| MSEVAPEEII | ENADGSRSII | TYKIEDGVKY | KITQKVKEVK | VLEKVHKSVA | ERKNWHKYGS |
| 70 | 80 | 90 | 100 | 110 | 120 |
| EKGSPAGPSA | VTARLGEEVE | LRLSRNWKQA | EEERIQKEKA | SLTKTGLQCR | LCGNDHMTMN |
| 130 | 140 | 150 | 160 | 170 | 180 |
| CPFKTILSEL | SALEDPATNE | GGVEAASEEK | AGQVGGAGSI | PGQYVPPSRR | AGARDPSSDA |
| 190 | 200 | 210 | 220 | 230 | 240 |
| YRDSRERDDM | CTLKIMQVNE | NADENSLREE | LLFPFAPIPR | VSVVRNKETG | KSRGLAFVTF |
| 250 | 260 | 270 | |||
| SSEEVAEQAL | RFLDGRGYMN | LILRVEWSKP | KVKE |