AiPD: Autoinhibited Protein Database

P59998

Gene name	ARPC4 (ARC20)
Protein name	Actin-related protein 2/3 complex subunit 4
Names	Arp2/3 complex 20 kDa subunit, p20-ARC
Species	Homo sapiens (Human)
KEGG Pathway	hsa:10093
EC number
Protein Class

Descriptions

The autoinhibited protein was predicted that may have potential autoinhibitory elements via cis-regPred.

Autoinhibitory domains (AIDs)

1-77 (Predicted disordered autoinhibitory region) PR

Target domain
Relief mechanism
Assay	cis-regPred

AID

1-77 (Predicted disordered autoinhibitory region)

Target domain

Relief mechanism

Assay

cis-regPred

Accessory elements

No accessory elements

References

Yeon JH et al. (2016) "Systems-wide Identification of cis-Regulatory Elements in Proteins", Cell Systems, 2, 89-100

Autoinhibited structure

Activated structure

5 structures for P59998

Entry ID	Method	Resolution	Chain	Position	Source
6UHC	EM	390 A	F	1-168	PDB
6YW6	EM	420 A	F	1-168	PDB
6YW7	EM	450 A	F	1-168	PDB
8P94	EM	330 A	F	1-168	PDB
AF-P59998-F1	Predicted				AlphaFoldDB

53 variants for P59998

Variant ID(s)	Position	Change	Description	Diseaes Association	Provenance
VAR_087805	158	R>C	DEVLO [UniProt]	Yes	UniProt
rs1268878299 CA351752234	2	T>I		No	ClinGen gnomAD
CA70015275 rs75791061	2	T>P		No	ClinGen Ensembl
CA351752246 rs1214607898	3	A>G		No	ClinGen gnomAD
CA2243883 rs779915246	3	A>T	Variant assessed as Somatic; 0.0 impact. [NCI-TCGA]	No	ClinGen ExAC NCI-TCGA gnomAD
CA70015280 rs923139837	5	L>V		No	ClinGen TOPMed
CA2243884 rs751354662	6	R>H		No	ClinGen ExAC TOPMed gnomAD
CA2243885 rs755000138	8	Y>C		No	ClinGen ExAC gnomAD
rs1171012441 CA351752337	10	S>N		No	ClinGen TOPMed
rs892928908 CA70015318	13	R>Q		No	ClinGen TOPMed gnomAD
rs1479160580 CA351752464	19	A>V		No	ClinGen TOPMed
rs771008114 CA2243891	20	L>V		No	ClinGen ExAC gnomAD
TCGA novel	27	S>missing	Variant assessed as Somatic; impact. [NCI-TCGA]	No	NCI-TCGA
rs902548379 CA70015346	28	Q>R		No	ClinGen Ensembl
TCGA novel	32	R>Q	Variant assessed as Somatic; impact. [NCI-TCGA]	No	NCI-TCGA
CA2243893 rs759934093	36	P>L		No	ClinGen ExAC gnomAD
rs1421619572 CA351691005	43	S>N		No	ClinGen TOPMed
rs753791120 CA2243926	44	K>R		No	ClinGen ExAC TOPMed gnomAD
CA69966626 rs775314716	49	Q>K		No	ClinGen Ensembl
rs757385458 CA2243927	51	V>A		No	ClinGen ExAC gnomAD
CA2243929 rs746095536	57	E>V		No	ClinGen ExAC TOPMed gnomAD
CA2243930 rs758559780	60	K>Q		No	ClinGen ExAC gnomAD
rs1559728178 CA351691365	61	V>F		No	ClinGen Ensembl
rs1340218076 CA351691371	62	L>R		No	ClinGen gnomAD
CA2243933 rs769037263	67	I>V		No	ClinGen ExAC TOPMed gnomAD
CA351691585 rs1559728199	71	R>Q		No	ClinGen Ensembl
CA351691722 rs1246032683	75	A>G		No	ClinGen gnomAD
rs777058198 CA2243936	78	Q>K		No	ClinGen ExAC
rs200029713 CA2243959	89	K>R		No	ClinGen 1000Genomes ExAC gnomAD
CA69968399 rs763004611	90	F>L		No	ClinGen Ensembl
TCGA novel	91	M>K	Variant assessed as Somatic; impact. [NCI-TCGA]	No	NCI-TCGA
rs760372060 CA2243960	91	M>L		No	ClinGen ExAC gnomAD
rs1238802545 CA351692788	92	R>L		No	ClinGen gnomAD
CA351692949 rs1323252912	95	M>I		No	ClinGen TOPMed gnomAD
rs768342495 CA2243961	100	N>K		No	ClinGen ExAC gnomAD
rs1209415192 CA351693169	103	I>V		No	ClinGen gnomAD
CA351693212 rs1371262365	105	R>*	Variant assessed as Somatic; impact. [NCI-TCGA]	No	ClinGen NCI-TCGA TOPMed
rs752920180 CA2243992	112	Y>C		No	ClinGen ExAC gnomAD
CA351694323 rs1212732224	114	I>V		No	ClinGen gnomAD
TCGA novel	118	I>V	Variant assessed as Somatic; impact. [NCI-TCGA]	No	NCI-TCGA
rs778216794 CA2243994	120	N>S		No	ClinGen ExAC gnomAD
rs754463207 CA2243995	121	F>V		No	ClinGen ExAC gnomAD
CA351694443 rs1241458167	125	Q>E		No	ClinGen gnomAD
CA351694473 rs1475096857	127	Y>C		No	ClinGen gnomAD
CA69970605 rs939481687	131	L>V		No	ClinGen Ensembl
rs746495713 CA2243998	141	E>Q		No	ClinGen ExAC gnomAD
rs754403614 CA2243999	145	E>G		No	ClinGen ExAC gnomAD
CA2244000 rs780842588	146	I>M		No	ClinGen ExAC gnomAD
CA2244002 rs769577281	152	S>L		No	ClinGen ExAC gnomAD
CA351694888 rs1353082956	156	R>H		No	ClinGen TOPMed gnomAD
rs749275458 CA351694929	159	I>N		No	ClinGen ExAC TOPMed gnomAD
rs749275458 CA2244004	159	I>T		No	ClinGen ExAC TOPMed gnomAD
rs1370896588 CA784121877	168	F>L		No	ClinGen TOPMed

No associated diseases with P59998

No regional properties for P59998

Type	Name	Position	InterPro Accession
No domain, repeats, and functional sites for P59998

Functions

		Description
EC Number
Subcellular Localization	Cytoplasm, cytoskeleton Cell projection Nucleus
PANTHER Family
PANTHER Subfamily
PANTHER Protein Class
PANTHER Pathway Category	No pathway information available

6 GO annotations of cellular component

Name	Definition
Arp2/3 protein complex	A stable protein complex that contains two actin-related proteins, Arp2 and Arp3, and five novel proteins (ARPC1-5), and functions in the nucleation of branched actin filaments.
cell projection	A prolongation or process extending from a cell, e.g. a flagellum or axon.
cytosol	The part of the cytoplasm that does not contain organelles but which does contain other particulate matter, such as protein complexes.
extracellular exosome	A vesicle that is released into the extracellular region by fusion of the limiting endosomal membrane of a multivesicular body with the plasma membrane. Extracellular exosomes, also simply called exosomes, have a diameter of about 40-100 nm.
nucleus	A membrane-bounded organelle of eukaryotic cells in which chromosomes are housed and replicated. In most cells, the nucleus contains all of the cell's chromosomes except the organellar chromosomes, and is the site of RNA synthesis and processing. In some species, or in specialized cell types, RNA metabolism or DNA replication may be absent.
site of double-strand break	A region of a chromosome at which a DNA double-strand break has occurred. DNA damage signaling and repair proteins accumulate at the lesion to respond to the damage and repair the DNA to form a continuous DNA helix.

3 GO annotations of molecular function

Name	Definition
actin binding	Binding to monomeric or multimeric forms of actin, including actin filaments.
enzyme binding	Binding to an enzyme, a protein with catalytic activity.
structural constituent of cytoskeleton	The action of a molecule that contributes to the structural integrity of a cytoskeletal structure.

4 GO annotations of biological process

Name	Definition
actin filament polymerization	Assembly of actin filaments by the addition of actin monomers to a filament.
actin nucleation	The initial step in the formation of an actin filament, in which actin monomers combine to form a new filament. Nucleation is slow relative to the subsequent addition of more monomers to extend the filament.
Arp2/3 complex-mediated actin nucleation	The actin nucleation process in which actin monomers combine to form a new branch on the side of an existing actin filament; mediated by the Arp2/3 protein complex and its interaction with other proteins.
regulation of actin filament polymerization	Any process that modulates the frequency, rate or extent of the assembly of actin filaments by the addition of actin monomers to a filament.

3 homologous proteins in AiPD

UniProt AC	Gene Name	Protein Name	Species	Evidence Code
Q148J6	ARPC4	Actin-related protein 2/3 complex subunit 4	Bos taurus (Bovine)	PR
P59999	Arpc4	Actin-related protein 2/3 complex subunit 4	Mus musculus (Mouse)	PR
P58798	arx-6	Probable actin-related protein 2/3 complex subunit 4	Caenorhabditis elegans	PR

10	20	30	40	50	60
MTATLRPYLS	AVRATLQAAL	CLENFSSQVV	ERHNKPEVEV	RSSKELLLQP	VTISRNEKEK
70	80	90	100	110	120
VLIEGSINSV	RVSIAVKQAD	EIEKILCHKF	MRFMMMRAEN	FFILRRKPVE	GYDISFLITN
130	140	150	160
FHTEQMYKHK	LVDFVIHFME	EIDKEISEMK	LSVNARARIV	AEEFLKNF