P32558

PR
Gene name
SPT16 (CDC68, SSF1, YGL207W)
Protein name
FACT complex subunit SPT16
Names
Cell division control protein 68, Facilitates chromatin transcription complex subunit SPT16, Suppressor of Ty protein 16
Species
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
KEGG Pathway
sce:YGL207W
EC number
Protein Class

Descriptions

The autoinhibited protein was predicted that may have potential autoinhibitory elements via cis-regPred.

Autoinhibitory domains (AIDs)

419-560 (Predicted disordered autoinhibitory region) PR

Target domain

Relief mechanism

Assay

cis-regPred

Accessory elements

No accessory elements

References

Autoinhibited structure

Activated structure

8 structures for P32558

Entry ID Method Resolution Chain Position Source
3BIP X-ray 194 A A/B 1-465 PDB
3BIQ X-ray 173 A A 1-465 PDB
3BIT X-ray 190 A A/B 1-451 PDB
4IOY X-ray 194 A X 675-958 PDB
4WNN X-ray 180 A T 958-972 PDB
7NKY EM 320 A Q 1-1035 PDB
8XGC EM 370 A L 1-1035 PDB
AF-P32558-F1 Predicted AlphaFoldDB

11 variants for P32558

Variant ID(s) Position Change Description Diseaes Association Provenance
s07-99174 68 V>I No SGRP
s07-99262 97 K>R No SGRP
s07-99286 105 R>K No SGRP
s07-99307 112 L>H No SGRP
s07-99330 120 V>I No SGRP
s07-100020 350 E>K No SGRP
s07-100114 381 V>A No SGRP
s07-100366 465 V>A No SGRP
s07-100533 521 A>T No SGRP
s07-100672 567 Y>C No SGRP
s07-101891 973 E>D No SGRP

No associated diseases with P32558

5 regional properties for P32558

Type Name Position InterPro Accession
domain Peptidase M24 200 - 422 IPR000994
domain Histone chaperone RTT106/FACT complex subunit SPT16-like, middle domain 834 - 924 IPR013719
domain FACT complex subunit Spt16 domain 553 - 707 IPR013953
domain FACT complex subunit Spt16, N-terminal lobe domain 6 - 169 IPR029148
domain FACT complex subunit Spt16, peptidase M24-like domain 184 - 435 IPR033825

Functions

Description
EC Number
Subcellular Localization
  • Nucleus
  • Chromosome
  • Colocalizes with RNA polymerase II on chromatin (PubMed:10413469, PubMed:14585989, PubMed:14739930)
  • Recruited to actively transcribed loci (PubMed:14585989)
  • Associates with the coding region of HTA1 (PubMed:19683497)
PANTHER Family
PANTHER Subfamily
PANTHER Protein Class
PANTHER Pathway Category No pathway information available

3 GO annotations of cellular component

Name Definition
chromatin The ordered and organized complex of DNA, protein, and sometimes RNA, that forms the chromosome.
FACT complex A histone chaperone complex that facilitates nucleosome disassembly and reassembly upon DNA or RNA polymerase passage.
replication fork protection complex A protein complex conserved in eukaryotes and associated with the replication fork; the complex stabilizes stalled replication forks and is thought to be involved in coordinating leading- and lagging-strand synthesis and in replication checkpoint signaling.

No GO annotations of molecular function

Name Definition
No GO annotations for molecular function

10 GO annotations of biological process

Name Definition
DNA repair The process of restoring DNA after damage. Genomes are subject to damage by chemical and physical agents in the environment (e.g. UV and ionizing radiations, chemical mutagens, fungal and bacterial toxins, etc.) and by free radicals or alkylating agents endogenously generated in metabolism. DNA is also damaged because of errors during its replication. A variety of different DNA repair pathways have been reported that include direct reversal, base excision repair, nucleotide excision repair, photoreactivation, bypass, double-strand break repair pathway, and mismatch repair pathway.
DNA-templated DNA replication A DNA replication process that uses parental DNA as a template for the DNA-dependent DNA polymerases that synthesize the new strands.
nucleosome assembly The aggregation, arrangement and bonding together of a nucleosome, the beadlike structural units of eukaryotic chromatin composed of histones and DNA.
nucleosome organization A process that is carried out at the cellular level which results in the assembly, arrangement of constituent parts, or disassembly of one or more nucleosomes.
positive regulation of RNA polymerase II transcription preinitiation complex assembly Any process that activates or increases the frequency, rate or extent of RNA polymerase II transcriptional preinitiation complex assembly.
positive regulation of transcription elongation by RNA polymerase II Any process that activates or increases the frequency, rate or extent of transcription elongation, the extension of an RNA molecule after transcription initiation and promoter clearance by the addition of ribonucleotides, catalyzed by RNA polymerase II.
positive regulation of transcription initiation by RNA polymerase II Any process that increases the rate, frequency or extent of a process involved in starting transcription from an RNA polymerase II promoter.
regulation of chromatin organization Any process that modulates the frequency, rate or extent of chromatin organization.
regulation of sister chromatid cohesion Any process that modulates the frequency, rate or extent of sister chromatid cohesion.
transcription elongation by RNA polymerase II promoter The extension of an RNA molecule after transcription initiation and promoter clearance at an RNA polymerase II promoter by the addition of ribonucleotides catalyzed by RNA polymerase II.

4 homologous proteins in AiPD

UniProt AC Gene Name Protein Name Species Evidence Code
Q9Y5B9 SUPT16H FACT complex subunit SPT16 Homo sapiens (Human) PR
Q8H6B1 SPT16 FACT complex subunit SPT16 Zea mays (Maize) PR
Q7X923 SPT16 FACT complex subunit SPT16 Oryza sativa subsp japonica (Rice) PR
O82491 SPT16 FACT complex subunit SPT16 Arabidopsis thaliana (Mouse-ear cress) PR
10 20 30 40 50 60
MEELNIDFDV FKKRIELLYS KYNEFEGSPN SLLFVLGSSN AENPYQKTTI LHNWLLSYEF
70 80 90 100 110 120
PATLIALVPG KVIIITSSAK AKHLQKAIDL FKDPESKITL ELWQRNNKEP ELNKKLFDDV
130 140 150 160 170 180
IALINSAGKT VGIPEKDSYQ GKFMTEWNPV WEAAVKENEF NVIDISLGLS KVWEVKDVNE
190 200 210 220 230 240
QAFLSVSSKG SDKFMDLLSN EMVRAVDEEL KITNAKLSDK IENKIDDVKF LKQLSPDLSA
250 260 270 280 290 300
LCPPNYKFNF DLLDWTYSPI IQSGKKFDLR VSARSTNDQL YGNGCILASC GIRYNNYCSN
310 320 330 340 350 360
ITRTFLIDPS EEMANNYDFL LTLQKEIVTN ILKPGRTPKE VYESVIEYIE KTKPELVPNF
370 380 390 400 410 420
TKNIGSLIGL EFRDSNFILN VKNDYRKIQR GDCFNISFGF NNLKDSQSAN NYALQLADTV
430 440 450 460 470 480
QIPLDETEPP RFLTNYTKAK SQISFYFNNE EEDNNKKKSS PATKVPSKPD RNSKILRTKL
490 500 510 520 530 540
RGEARGGAED AQKEQIRKEN QKKLHEKLEK NGLLRFSAAD ANGPDSEPRQ YFKKYESYVR
550 560 570 580 590 600
DSQLPTNIRD LRIHVDWKSQ TIILPIYGRP VPFHINSYKN GSKNEEGEYT YLRLNFNSPG
610 620 630 640 650 660
SSGGISKKVE ELPYEESADN QFVRSITLRS KDGDRMSETF KQIADLKKEA TKREQERKAL
670 680 690 700 710 720
ADVVQQDKLI ENKTGRTKRL DQIFVRPNPD TKRVPSTVFI HENGIRFQSP LRTDSRIDIL
730 740 750 760 770 780
FSNIKNLIFQ SCKGELIVVI HIHLKNPILM GKKKIQDVQF YREASDMSVD ETGGGRRGQS
790 800 810 820 830 840
RFRRYGDEDE LEQEQEERRK RAALDKEFKY FADAIAEASN GLLTVENTFR DLGFQGVPNR
850 860 870 880 890 900
SAVFCMPTTD CLVQLIEPPF LVINLEEVEI CILERVQFGL KNFDMVFVYK DFNKPVTHIN
910 920 930 940 950 960
TVPIESLDFL KQWLTDMDIP YTVSTINLNW ATIMKSLQDD PYQFFLDGGW NFLATGSDDE
970 980 990 1000 1010 1020
ASDESEEEVS EYEASEDDVS DESAFSEDEE GSEVDDDISG DESEDYTGDE SEEGEDWDEL
1030
EKKAARADRG ANFRD