P30750

PR
Gene name
metN
Protein name
Methionine import ATP-binding protein MetN
Names
Species
Escherichia coli (strain K12)
KEGG Pathway
eco:b0199
EC number
7.4.2.11: Linked to the hydrolysis of a nucleoside triphosphate
Protein Class

Descriptions

The autoinhibited protein was predicted that may have potential autoinhibitory elements via cis-regPred.

Autoinhibitory domains (AIDs)

85-173 (Predicted disordered autoinhibitory region) PR

Target domain

Relief mechanism

Assay

cis-regPred

Accessory elements

No accessory elements

References

Autoinhibited structure

Activated structure

7 structures for P30750

Entry ID Method Resolution Chain Position Source
3DHW X-ray 370 A C/D/G/H 1-343 PDB
3DHX X-ray 210 A A/B 246-343 PDB
3TUI X-ray 290 A C/D/G/H 1-343 PDB
3TUJ X-ray 400 A C/D 1-343 PDB
3TUZ X-ray 340 A C/D/G/H 1-343 PDB
6CVL X-ray 295 A C/D 1-343 PDB
AF-P30750-F1 Predicted AlphaFoldDB

No variants for P30750

Variant ID(s) Position Change Description Diseaes Association Provenance
No variants for P30750

No associated diseases with P30750

5 regional properties for P30750

Type Name Position InterPro Accession
domain ABC transporter-like, ATP-binding domain 2 - 241 IPR003439
domain AAA+ ATPase domain 30 - 218 IPR003593
conserved_site ABC transporter-like, conserved site 141 - 155 IPR017871
domain NIL domain 265 - 340 IPR018449
domain Methionine import ATP-binding protein MetN, ATP-binding domain 1 - 233 IPR041701

Functions

Description
EC Number 7.4.2.11 Linked to the hydrolysis of a nucleoside triphosphate
Subcellular Localization
  • Cell inner membrane ; Peripheral membrane protein
PANTHER Family
PANTHER Subfamily
PANTHER Protein Class
PANTHER Pathway Category No pathway information available

4 GO annotations of cellular component

Name Definition
ATP-binding cassette (ABC) transporter complex, substrate-binding subunit-containing A complex for the transport of metabolites into the cell, consisting of 5 subunits: two ATP-binding subunits, two membrane spanning subunits, and one substrate-binding subunit. In organisms with two membranes, the substrate-binding protein moves freely in the periplasmic space and joins the other subunits only when bound with substrate. In organisms with only one membrane the substrate-binding protein is tethered to the cytoplasmic membrane and associated with the other subunits. Transport of the substrate across the membrane is driven by the hydrolysis of ATP.
Gram-negative-bacterium-type cell wall The peptidoglycan layer of the Gram-negative cell envelope. In Gram-negative cells the peptidoglycan is relatively thin (1-2nm) and is linked to the outer membrane by lipoproteins. In Gram-negative cells the peptidoglycan is too thin to retain the primary stain in the Gram staining procedure and therefore cells appear red after Gram stain.
membrane A lipid bilayer along with all the proteins and protein complexes embedded in it an attached to it.
methionine-importing ABC transporter complex An ATP-binding cassette (ABC) transporter complex that is capable of methionine-importing activity. An example is the bacterial MetNIQ methionine transporter, that consists of the dimeric ATPase subunit MetN located at the cytoplasmic side of the plasma membrane and the dimeric transmembrane subunit MetI. MetQ is regarded as the periplasmic methionine-binding chaperon subunit, and is capable of transporting methionine from the periplasm into the cytoplasm in an ATP-dependent manner.

5 GO annotations of molecular function

Name Definition
ABC-type D-methionine transporter activity Enables the transfer of a solute or solutes from one side of a membrane to the other according to the reaction: ATP + H2O + D-methionine(out/in) = ADP + phosphate + D-methionine(in/out).
ATP binding Binding to ATP, adenosine 5'-triphosphate, a universally important coenzyme and enzyme regulator.
ATP hydrolysis activity Catalysis of the reaction: ATP + H2O = ADP + H+ phosphate. ATP hydrolysis is used in some reactions as an energy source, for example to catalyze a reaction or drive transport against a concentration gradient.
ATPase-coupled transmembrane transporter activity Primary active transporter of a solute across a membrane, via the reaction: ATP + H2O = ADP + phosphate, to directly drive the transport of a substance across a membrane. The transport protein may be transiently phosphorylated (P-type transporters), or not (ABC-type transporters and other families of transporters). Primary active transport occurs up the solute's concentration gradient and is driven by a primary energy source.
L-methionine transmembrane transporter activity Enables the transfer of L-methionine from one side of a membrane to the other. L-methionine is 2-amino-4-(methylthio)butanoic acid.

3 GO annotations of biological process

Name Definition
D-methionine transport The directed movement of D-methionine into, out of, within, or between cells.
metabolic process The chemical reactions and pathways, including anabolism and catabolism, by which living organisms transform chemical substances. Metabolic processes typically transform small molecules, but also include macromolecular processes such as DNA repair and replication, and protein synthesis and degradation.
methionine import across plasma membrane The directed movement of methionine from outside of a cell, across the plasma membrane and into the cytosol.

3 homologous proteins in AiPD

UniProt AC Gene Name Protein Name Species Evidence Code
P07109 hisP Histidine transport ATP-binding protein HisP Escherichia coli (strain K12) PR
P37774 tcyN L-cystine transport system ATP-binding protein TcyN Escherichia coli (strain K12) PR
P10346 glnQ Glutamine transport ATP-binding protein GlnQ Escherichia coli (strain K12) PR
10 20 30 40 50 60
MIKLSNITKV FHQGTRTIQA LNNVSLHVPA GQIYGVIGAS GAGKSTLIRC VNLLERPTEG
70 80 90 100 110 120
SVLVDGQELT TLSESELTKA RRQIGMIFQH FNLLSSRTVF GNVALPLELD NTPKDEVKRR
130 140 150 160 170 180
VTELLSLVGL GDKHDSYPSN LSGGQKQRVA IARALASNPK VLLCDEATSA LDPATTRSIL
190 200 210 220 230 240
ELLKDINRRL GLTILLITHE MDVVKRICDC VAVISNGELI EQDTVSEVFS HPKTPLAQKF
250 260 270 280 290 300
IQSTLHLDIP EDYQERLQAE PFTDCVPMLR LEFTGQSVDA PLLSETARRF NVNNNIISAQ
310 320 330 340
MDYAGGVKFG IMLTEMHGTQ QDTQAAIAWL QEHHVKVEVL GYV