P22817

PR
Gene name
Ide (CG5517)
Protein name
Insulin-degrading enzyme
Names
Insulin protease, Insulinase, Insulysin
Species
Drosophila melanogaster (Fruit fly)
KEGG Pathway
dme:Dmel_CG5517
EC number
3.4.24.56: Metalloendopeptidases
Protein Class

Descriptions

The autoinhibited protein was predicted that may have potential autoinhibitory elements via cis-regPred.

Autoinhibitory domains (AIDs)

1-61 (Predicted disordered autoinhibitory region) PR

Target domain

Relief mechanism

Assay

cis-regPred

Accessory elements

No accessory elements

References

Autoinhibited structure

Activated structure

1 structures for P22817

Entry ID Method Resolution Chain Position Source
AF-P22817-F1 Predicted AlphaFoldDB

No variants for P22817

Variant ID(s) Position Change Description Diseaes Association Provenance
No variants for P22817

No associated diseases with P22817

5 regional properties for P22817

Type Name Position InterPro Accession
binding_site Peptidase M16, zinc-binding site 68 - 91 IPR001431
domain Peptidase M16, C-terminal 211 - 387 IPR007863-1
domain Peptidase M16, C-terminal 680 - 863 IPR007863-2
domain Peptidase M16, N-terminal 47 - 185 IPR011765
domain Peptidase M16, middle/third domain 395 - 676 IPR032632

Functions

Description
EC Number 3.4.24.56 Metalloendopeptidases
Subcellular Localization
PANTHER Family
PANTHER Subfamily
PANTHER Protein Class
PANTHER Pathway Category No pathway information available

5 GO annotations of cellular component

Name Definition
cytoplasm The contents of a cell excluding the plasma membrane and nucleus, but including other subcellular structures.
cytosol The part of the cytoplasm that does not contain organelles but which does contain other particulate matter, such as protein complexes.
mitochondrion A semiautonomous, self replicating organelle that occurs in varying numbers, shapes, and sizes in the cytoplasm of virtually all eukaryotic cells. It is notably the site of tissue respiration.
peroxisome A small organelle enclosed by a single membrane, and found in most eukaryotic cells. Contains peroxidases and other enzymes involved in a variety of metabolic processes including free radical detoxification, lipid catabolism and biosynthesis, and hydrogen peroxide metabolism.
plasma membrane The membrane surrounding a cell that separates the cell from its external environment. It consists of a phospholipid bilayer and associated proteins.

3 GO annotations of molecular function

Name Definition
metal ion binding Binding to a metal ion.
metalloendopeptidase activity Catalysis of the hydrolysis of internal, alpha-peptide bonds in a polypeptide chain by a mechanism in which water acts as a nucleophile, one or two metal ions hold the water molecule in place, and charged amino acid side chains are ligands for the metal ions.
metallopeptidase activity Catalysis of the hydrolysis of peptide bonds by a mechanism in which water acts as a nucleophile, one or two metal ions hold the water molecule in place, and charged amino acid side chains are ligands for the metal ions.

12 GO annotations of biological process

Name Definition
defense response to Gram-negative bacterium Reactions triggered in response to the presence of a Gram-negative bacterium that act to protect the cell or organism.
determination of adult lifespan The pathways that regulate the duration of the adult phase of the life-cycle of an animal.
insulin catabolic process The chemical reactions and pathways resulting in the breakdown of insulin.
negative regulation of developmental growth Any process that stops, prevents, or reduces the frequency, rate or extent of developmental growth.
negative regulation of growth Any process that stops, prevents or reduces the rate or extent of growth, the increase in size or mass of all or part of an organism.
negative regulation of insulin receptor signaling pathway Any process that stops, prevents, or reduces the frequency, rate or extent of insulin receptor signaling.
peptide catabolic process The chemical reactions and pathways resulting in the breakdown of peptides, compounds of 2 or more (but usually less than 100) amino acids where the alpha carboxyl group of one is bound to the alpha amino group of another.
positive regulation of innate immune response Any process that activates or increases the frequency, rate or extent of the innate immune response, the organism's first line of defense against infection.
proteolysis The hydrolysis of proteins into smaller polypeptides and/or amino acids by cleavage of their peptide bonds.
proteolysis involved in protein catabolic process The hydrolysis of a peptide bond or bonds within a protein as part of the chemical reactions and pathways resulting in the breakdown of a protein by individual cells.
regulation of oviposition Any process that modulates the frequency, rate or extent of the deposition of eggs, either fertilized or not, upon a surface or into a medium.
regulation of trehalose metabolic process Any process that modulates the frequency, rate or extent of trehalose metabolism, the chemical reactions and pathways involving trehalose, a disaccharide isomeric with sucrose and obtained from certain lichens and fungi.

2 homologous proteins in AiPD

UniProt AC Gene Name Protein Name Species Evidence Code
Q06010 STE23 A-factor-processing enzyme Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) PR
Q10040 C28F5.4 Putative zinc protease C28F5.4 Caenorhabditis elegans PR
10 20 30 40 50 60
MTIAESSQKS ATRKPDSMEP ILRLNNIEKS LQDTRDYRGL QLENGLKVLL ISDPNTDVSA
70 80 90 100 110 120
AALSVQVGHM SDPTNLPGLA HFCEHMLFLG TEKYPHENGY TTYLSQSGGS SNAATYPLMT
130 140 150 160 170 180
KYHFHVAPDK LDGALDRFAQ FFIAPLFTPS ATEREINAVN SEHEKNLPSD LWRIKQVNRH
190 200 210 220 230 240
LAKPDHAYSK FGSGNKTTLS EIPKSKNIDV RDELLKFHKQ WYSANIMCLA VIGKESLDEL
250 260 270 280 290 300
EGMVLEKFSE IENKNVKVPG WPRHPYAEER YGQKVKIVPI KDIRSLTISF TTDDLTQFYK
310 320 330 340 350 360
SGPDNYLTHL IGHEGKGSIL SELRRLGWCN DLMAGHQNTQ NGFGFFDIVV DLTQEGLEHV
370 380 390 400 410 420
DDIVKIVFQY LEMLRKEGPK KWIFDECVKL NEMRFRFKEK EQPENLVTHA VSSMQIFPLE
430 440 450 460 470 480
EVLIAPYLSN EWRPDLIKGL LDELVPSKSR IVIVSQSFEP DCDLAEPYYK TKYGITRVAK
490 500 510 520 530 540
DTVQSWENCE LNENLKLALP NSFIPTNFDI SDVPADAPKH PTIILDTPIL RVWHKQDNQF
550 560 570 580 590 600
NKPKACMTFD MSNPIAYLDP LNCNLNHMMV MLLKDQLNEY LYDAELASLK LSVMGKSCGI
610 620 630 640 650 660
DFTIRGFSDK QVVLLEKLLD HLFDFSIDEK RFDILKEEYV RSLKNFKAEQ PYQHSIYYLA
670 680 690 700 710 720
LLLTENAWAN MELLDAMELV TYDRVLNFAK EFFQRLHTEC FIFGNVTKQQ ATDIAGRVNT
730 740 750 760 770 780
RLEATNASKL PILARQMLKK REYKLLAGDS YLFEKENEFH KSSCAQLYLQ CGAQTDHTNI
790 800 810 820 830 840
MVNLVSQVLS EPCYDCLRTK EQLGYIVFSG VRKVNGANGI RIIVQSAKHP SYVEDRIENF
850 860 870 880 890 900
LQTYLQVIED MPLDEFERHK EALAVKKLEK PKTIFQQFSQ FYGEIAMQTY HFEREEAEVA
910 920 930 940 950 960
ILRKISKADF VDYFKKFIAK DGEERRVLSV HIVSQQTDEN ATSEAEPVEI TNMERHKPIS
970 980
DIVTFKSCKE LYPIALPFLD IKAKGARSKL