AiPD: Autoinhibited Protein Database

P20827

Gene name	EFNA1 (EPLG1, LERK1, TNFAIP4)
Protein name	Ephrin-A1
Names	EPH-related receptor tyrosine kinase ligand 1, LERK-1, Immediate early response protein B61, Tumor necrosis factor alpha-induced protein 4, TNF alpha-induced protein 4
Species	Homo sapiens (Human)
KEGG Pathway	hsa:1942
EC number
Protein Class

Descriptions

The autoinhibited protein was predicted that may have potential autoinhibitory elements via cis-regPred.

Autoinhibitory domains (AIDs)

118-192 (Predicted disordered autoinhibitory region) PR

Target domain
Relief mechanism
Assay	cis-regPred

AID

118-192 (Predicted disordered autoinhibitory region)

Target domain

Relief mechanism

Assay

cis-regPred

Accessory elements

No accessory elements

References

Yeon JH et al. (2016) "Systems-wide Identification of cis-Regulatory Elements in Proteins", Cell Systems, 2, 89-100

Autoinhibited structure

Activated structure

4 structures for P20827

Entry ID	Method	Resolution	Chain	Position	Source
3CZU	X-ray	265 A	B	17-171	PDB
3HEI	X-ray	200 A	B/D/F/H/J/L/N/P	18-147	PDB
3MBW	X-ray	281 A	B	17-171	PDB
AF-P20827-F1	Predicted				AlphaFoldDB

161 variants for P20827

Variant ID(s)	Position	Change	Description	Diseaes Association	Provenance
rs1424951930 CA342642401	3	F>Y		No	ClinGen gnomAD
CA1137966 rs779147208	4	L>F		No	ClinGen ExAC TOPMed gnomAD
rs746107000 CA1137967	4	L>H		No	ClinGen ExAC TOPMed gnomAD
rs372639058 CA1137969	7	P>S		No	ClinGen ESP ExAC TOPMed gnomAD
rs372639058 CA1137968	7	P>T		No	ClinGen ESP ExAC TOPMed gnomAD
CA342642491 rs535261907	8	L>F		No	ClinGen 1000Genomes ExAC gnomAD
rs535261907 CA1137970	8	L>I		No	ClinGen 1000Genomes ExAC gnomAD
CA342642494 rs535261907	8	L>V		No	ClinGen 1000Genomes ExAC gnomAD
rs1374857084 CA342642544	10	G>A		No	ClinGen gnomAD
rs1355150575 CA342642537	10	G>R		No	ClinGen TOPMed
TCGA novel	11	L>M	Variant assessed as Somatic; impact. [NCI-TCGA]	No	NCI-TCGA
TCGA novel	12	C>R	Variant assessed as Somatic; impact. [NCI-TCGA]	No	NCI-TCGA
rs777172181 CA1137973	14	S>N		No	ClinGen ExAC gnomAD
CA1137972 rs769122314	14	S>R		No	ClinGen ExAC gnomAD
CA342642663 rs1390785242	15	L>V		No	ClinGen gnomAD
CA1137974 rs747650541	16	A>S		No	ClinGen ExAC gnomAD
CA1137975 rs769221879	16	A>V		No	ClinGen ExAC gnomAD
rs1226025324 CA342642794	20	R>L		No	ClinGen gnomAD
CA342642815 rs1308299533	21	H>Y		No	ClinGen TOPMed gnomAD
CA342642884 rs200194706	24	F>L		No	ClinGen 1000Genomes TOPMed gnomAD
rs1324914487 CA342642915	25	W>*		No	ClinGen TOPMed
CA342642902 rs1256119888	25	W>*		No	ClinGen gnomAD
CA30855113 rs369005847	27	S>G		No	ClinGen ESP TOPMed
rs766190644 CA1137978	27	S>R		No	ClinGen ExAC gnomAD
rs774280888 CA1137979	30	P>R		No	ClinGen ExAC TOPMed gnomAD
CA1137980 rs79752142	31	K>R		No	ClinGen 1000Genomes ExAC gnomAD
CA1137981 rs79752142	31	K>T		No	ClinGen 1000Genomes ExAC gnomAD
rs144975407 CA1138009	33	R>Q		No	ClinGen ESP ExAC TOPMed gnomAD
rs145089843 CA1138008	33	R>W		No	ClinGen 1000Genomes ESP ExAC TOPMed gnomAD
rs750439512 CA342644534	34	N>I		No	ClinGen ExAC gnomAD
CA1138010 rs750439512	34	N>S		No	ClinGen ExAC gnomAD
rs1320581827 CA342644543	35	E>K		No	ClinGen gnomAD
CA1138011 rs763069837	36	D>N		No	ClinGen ExAC TOPMed gnomAD
rs755272713 CA1138014	39	I>V		No	ClinGen ExAC
rs138253207 CA1138016 CA1138018	40	H>Q		No	ClinGen ESP ExAC TOPMed gnomAD
rs781427172 CA1138015	40	H>R		No	ClinGen ExAC gnomAD
rs1488116519 CA342644771	43	L>P		No	ClinGen gnomAD
CA30857993 rs146875946	46	Y>C		No	ClinGen ESP TOPMed gnomAD
CA1138020 rs376157103	47	V>M		No	ClinGen ESP ExAC TOPMed gnomAD
CA342644870 rs1267793423	48	D>E		No	ClinGen TOPMed
rs374457600 CA1138021	49	I>V		No	ClinGen ESP ExAC TOPMed gnomAD
rs1358769832 CA342644930	51	C>Y		No	ClinGen TOPMed
rs1285652444 CA342644950	52	P>L		No	ClinGen gnomAD
TCGA novel	54	Y>H	Variant assessed as Somatic; impact. [NCI-TCGA]	No	NCI-TCGA
CA342645067 rs1571685314	57	H>P		No	ClinGen Ensembl
TCGA novel	57	H>R	Variant assessed as Somatic; impact. [NCI-TCGA]	No	NCI-TCGA
rs760560336 CA1138025	59	V>M		No	ClinGen ExAC gnomAD
CA1138026 rs768457240	60	A>V		No	ClinGen ExAC TOPMed gnomAD
CA30858066 rs560935260	61	D>G		No	ClinGen Ensembl
rs762981577 CA342645184	62	A>P		No	ClinGen ExAC TOPMed gnomAD
rs762981577 CA1138028	62	A>T	Variant assessed as Somatic; 0.0 impact. [NCI-TCGA]	No	ClinGen ExAC NCI-TCGA TOPMed gnomAD
CA342645378 rs1377712269	68	I>M		No	ClinGen gnomAD
rs942046385 CA30858074	72	V>A		No	ClinGen Ensembl
rs551006267 CA1138031	73	E>K		No	ClinGen 1000Genomes ExAC TOPMed gnomAD
rs551006267 CA1138030	73	E>Q		No	ClinGen 1000Genomes ExAC TOPMed gnomAD
CA1138033 rs780550861	75	E>V		No	ClinGen ExAC gnomAD
TCGA novel	76	E>*	Variant assessed as Somatic; impact. [NCI-TCGA]	No	NCI-TCGA
CA342645598 rs1571685393	77	Y>S		No	ClinGen Ensembl
CA1138034 rs752969888	79	L>R		No	ClinGen ExAC gnomAD
TCGA novel	79	L>V	Variant assessed as Somatic; impact. [NCI-TCGA]	No	NCI-TCGA
CA342645794 rs1473764086	83	Q>*		No	ClinGen gnomAD
CA342645800 rs1205239967	83	Q>P		No	ClinGen TOPMed gnomAD
rs1205239967 CA342645802	83	Q>R		No	ClinGen TOPMed gnomAD
TCGA novel	83	Q>missing	Variant assessed as Somatic; impact. [NCI-TCGA]	No	NCI-TCGA
CA1138038 rs754426787	89	R>C		No	ClinGen ExAC TOPMed gnomAD
CA1138039 rs569517236	89	R>H		No	ClinGen 1000Genomes ExAC TOPMed gnomAD
rs1360527838 CA342646007	90	W>*		No	ClinGen gnomAD
rs778578434 CA1138040	91	Q>H		No	ClinGen ExAC gnomAD
CA342646099 rs1357900845	93	N>K		No	ClinGen TOPMed gnomAD
CA1138041 rs745426151	93	N>S		No	ClinGen ExAC gnomAD
rs745426151 CA1138042	93	N>T		No	ClinGen ExAC gnomAD
rs868264737 CA30858173	94	R>L		No	ClinGen TOPMed gnomAD
rs868264737 CA30858148	94	R>Q		No	ClinGen TOPMed gnomAD
rs1450856797 CA342646108	94	R>W		No	ClinGen TOPMed gnomAD
rs779806956 CA1138043	95	P>S		No	ClinGen ExAC TOPMed gnomAD
CA342646193 rs1238160787	97	A>V		No	ClinGen TOPMed gnomAD
CA342646271 rs1325110039	100	G>S		No	ClinGen TOPMed
rs746733925 CA1138044	101	P>L		No	ClinGen ExAC TOPMed gnomAD
CA342646396 rs1444026141	104	L>V		No	ClinGen gnomAD
rs748101140 CA1138047	109	Q>P		No	ClinGen ExAC gnomAD
rs548758015 CA1138048 COSM2209912	110	R>C	Variant assessed as Somatic; 0.0 impact. large_intestine [NCI-TCGA, Cosmic]	No	ClinGen cosmic curated 1000Genomes ExAC NCI-TCGA TOPMed gnomAD
rs78742081 CA1138049	110	R>H		No	ClinGen 1000Genomes ESP ExAC TOPMed gnomAD
rs1380511979 CA342646668	116	L>P		No	ClinGen gnomAD
rs1170256544 CA342646672	117	G>S		No	ClinGen gnomAD
CA342646689 rs1426696305	118	K>E		No	ClinGen gnomAD
TCGA novel	120	F>L	Variant assessed as Somatic; impact. [NCI-TCGA]	No	NCI-TCGA
rs1400298011 CA342646779	122	E>G		No	ClinGen gnomAD
rs759698206 CA342646771	122	E>K		No	ClinGen ExAC gnomAD
rs759698206 CA1138051	122	E>Q		No	ClinGen ExAC gnomAD
rs373761359 CA1138052	124	H>Q		No	ClinGen ESP ExAC TOPMed gnomAD
CA342646816 rs1362549200	124	H>R		No	ClinGen Ensembl
CA1138053 rs775799339	126	Y>N		No	ClinGen ExAC gnomAD
TCGA novel	129	I>=	Variant assessed as Somatic; impact. [NCI-TCGA]	No	NCI-TCGA
TCGA novel	130	S>F	Variant assessed as Somatic; impact. [NCI-TCGA]	No	NCI-TCGA
rs773180386 CA1138069	131	K>E		No	ClinGen ExAC gnomAD
rs1571686981 CA342648145	132	P>S		No	ClinGen Ensembl
CA342648213 rs1258407591	134	H>Q		No	ClinGen TOPMed gnomAD
rs745882383 CA1138070	136	H>R		No	ClinGen ExAC
rs772220878 CA1138071	137	E>K		No	ClinGen ExAC gnomAD
rs775507556 CA1138072	137	E>V		No	ClinGen ExAC TOPMed gnomAD
rs1451875266 CA342648346 COSM896773	139	R>C	Variant assessed as Somatic; 0.0 impact. endometrium [NCI-TCGA, Cosmic]	No	ClinGen cosmic curated NCI-TCGA gnomAD
rs760965678 CA1138073	139	R>H		No	ClinGen ExAC TOPMed gnomAD
COSM1334553 CA1138074 rs760965678	139	R>L	Variant assessed as Somatic; 0.0 impact. large_intestine [NCI-TCGA, Cosmic]	No	ClinGen cosmic curated ExAC NCI-TCGA TOPMed gnomAD
CA1138076 rs143462709	142	R>K		No	ClinGen ESP ExAC TOPMed gnomAD
rs750972580 CA1138079	143	L>V		No	ClinGen ExAC gnomAD
CA30859398 rs11545256	145	V>A		No	ClinGen Ensembl
CA1138081 rs751092470	146	T>I		No	ClinGen ExAC TOPMed gnomAD
rs991598289 CA30859418	147	V>A		No	ClinGen TOPMed
CA342648540 rs1571687089	148	S>R		No	ClinGen Ensembl
TCGA novel	149	G>D	Variant assessed as Somatic; impact. [NCI-TCGA]	No	NCI-TCGA
CA342648565 rs1393115564	149	G>S		No	ClinGen gnomAD
CA342648595 rs1273315508	150	K>T		No	ClinGen gnomAD
CA30859613 rs201040880	153	H>L		No	ClinGen gnomAD
CA30859610 rs755349435	153	H>Y		No	ClinGen Ensembl
rs1476226765 CA342648813	154	S>G		No	ClinGen gnomAD
rs1332131897 CA342648871	156	Q>E		No	ClinGen TOPMed
CA342648899 rs1375907579	157	A>D		No	ClinGen TOPMed
CA342648935 rs1166042953	158	H>Q		No	ClinGen gnomAD
CA30859618 rs866318420	159	D>N		No	ClinGen Ensembl
rs4745 CA1138097 VAR_014791	159	D>V		No	ClinGen UniProt 1000Genomes ESP ExAC TOPMed dbSNP gnomAD
rs375381523 CA30859631	161	P>T		No	ClinGen Ensembl
rs1388514930 CA342649089	164	K>E		No	ClinGen TOPMed
CA342649099 rs1238889558	164	K>N		No	ClinGen gnomAD
rs773535406 CA342649094	164	K>R		No	ClinGen ExAC TOPMed gnomAD
rs773535406 CA1138099	164	K>T		No	ClinGen ExAC TOPMed gnomAD
CA342649144 rs1475793891	166	L>F		No	ClinGen TOPMed
rs1355475807 CA342649174	167	A>E		No	ClinGen TOPMed gnomAD
rs1355475807 CA342649177	167	A>G		No	ClinGen TOPMed gnomAD
rs1355475807 CA342649173	167	A>V	Variant assessed as Somatic; impact. [NCI-TCGA]	No	ClinGen NCI-TCGA TOPMed gnomAD
rs1234872058 CA342650650	172	E>D		No	ClinGen gnomAD
rs114708813 CA1138117	172	E>K		No	ClinGen 1000Genomes ESP ExAC TOPMed gnomAD
CA342650684 rs139969988	174	R>P		No	ClinGen 1000Genomes ESP ExAC TOPMed gnomAD
CA1138120 rs139969988	174	R>Q		No	ClinGen 1000Genomes ESP ExAC TOPMed gnomAD
CA1138119 rs770201963	174	R>W		No	ClinGen ExAC TOPMed gnomAD
rs771450050 CA1138122	175	V>A		No	ClinGen ExAC TOPMed gnomAD
rs1481713806 CA342650695	175	V>I		No	ClinGen gnomAD
CA30859924 rs917364523	177	H>R		No	ClinGen TOPMed gnomAD
rs1178396431 CA342650783	178	S>T		No	ClinGen gnomAD
rs17855563 CA30859936	180	G>A		No	ClinGen Ensembl
rs767014352 COSM3385227 CA1138125	180	G>S	pancreas [Cosmic]	No	ClinGen cosmic curated ExAC gnomAD
CA1138126 rs775062336	181	H>Q		No	ClinGen ExAC gnomAD
CA342650928 rs1442400747	182	S>R		No	ClinGen gnomAD
TCGA novel	184	A>S	Variant assessed as Somatic; impact. [NCI-TCGA]	No	NCI-TCGA
CA342651002 rs1292581354	185	P>L		No	ClinGen gnomAD
CA1138127 rs760306344	186	R>C		No	ClinGen ExAC TOPMed gnomAD
rs201402574 CA1138128	186	R>H		No	ClinGen ESP ExAC TOPMed gnomAD
rs201402574 CA1138129	186	R>P		No	ClinGen ESP ExAC TOPMed gnomAD
rs765183519 CA1138131	188	F>L		No	ClinGen ExAC gnomAD
rs750393153 CA1138132	190	L>F		No	ClinGen ExAC gnomAD
CA1138133 rs755051226	190	L>P		No	ClinGen ExAC TOPMed gnomAD
rs781409409 CA342651174	191	A>S		No	ClinGen ExAC TOPMed gnomAD
CA1138134 rs781409409	191	A>T		No	ClinGen ExAC TOPMed gnomAD
rs1205744829 CA342651190	191	A>V		No	ClinGen gnomAD
rs748308594 CA1138135	193	T>N		No	ClinGen ExAC gnomAD
rs959193434 CA30859976	194	V>A		No	ClinGen TOPMed gnomAD
COSM139319 rs1395238113 CA342651330	196	L>F	skin [Cosmic]	No	ClinGen cosmic curated TOPMed
TCGA novel	198	P>A	Variant assessed as Somatic; impact. [NCI-TCGA]	No	NCI-TCGA
rs777908694 CA1138137	199	L>F		No	ClinGen ExAC gnomAD
CA1138138 rs777908694	199	L>V		No	ClinGen ExAC gnomAD
CA30860013 rs1027606030	202	L>P		No	ClinGen gnomAD
rs746384002 CA1138141	205	P>L		No	ClinGen ExAC gnomAD

No associated diseases with P20827

3 regional properties for P20827

Type	Name	Position	InterPro Accession
domain	Ephrin receptor-binding domain	17 - 151	IPR001799
conserved_site	Ephrin, conserved site	103 - 130	IPR019765
domain	Ephrin-A ectodomain	19 - 148	IPR034252

Functions

		Description
EC Number
Subcellular Localization	Cell membrane ; Lipid-anchor, GPI-anchor ;
PANTHER Family
PANTHER Subfamily
PANTHER Protein Class
PANTHER Pathway Category	No pathway information available

3 GO annotations of cellular component

Name	Definition
anchored component of plasma membrane	The component of the plasma membrane consisting of the gene products that are tethered to the membrane only by a covalently attached anchor, such as a lipid group, that is embedded in the membrane. Gene products with peptide sequences that are embedded in the membrane are excluded from this grouping.
extracellular region	The space external to the outermost structure of a cell. For cells without external protective or external encapsulating structures this refers to space outside of the plasma membrane. This term covers the host cell environment outside an intracellular parasite.
plasma membrane	The membrane surrounding a cell that separates the cell from its external environment. It consists of a phospholipid bilayer and associated proteins.

2 GO annotations of molecular function

Name	Definition
ephrin receptor binding	Binding to an ephrin receptor.
signaling receptor binding	Binding to one or more specific sites on a receptor molecule, a macromolecule that undergoes combination with a hormone, neurotransmitter, drug or intracellular messenger to initiate a change in cell function.

28 GO annotations of biological process

Name	Definition
angiogenesis	Blood vessel formation when new vessels emerge from the proliferation of pre-existing blood vessels.
aortic valve morphogenesis	The process in which the structure of the aortic valve is generated and organized.
axon guidance	The chemotaxis process that directs the migration of an axon growth cone to a specific target site in response to a combination of attractive and repulsive cues.
cell migration	The controlled self-propelled movement of a cell from one site to a destination guided by molecular cues. Cell migration is a central process in the development and maintenance of multicellular organisms.
cell-cell signaling	Any process that mediates the transfer of information from one cell to another. This process includes signal transduction in the receiving cell and, where applicable, release of a ligand and any processes that actively facilitate its transport and presentation to the receiving cell. Examples include signaling via soluble ligands, via cell adhesion molecules and via gap junctions.
endocardial cushion to mesenchymal transition involved in heart valve formation	A transition where an endocardial cushion cell loses apical/basolateral polarity, severs intercellular adhesive junctions, degrades basement membrane components and becomes a migratory mesenchymal cell that will contribute to the formation of a cardiac valve.
ephrin receptor signaling pathway	The series of molecular signals initiated by ephrin binding to its receptor, and ending with the regulation of a downstream cellular process, e.g. transcription.
mitral valve morphogenesis	The process in which the structure of the mitral valve is generated and organized.
negative regulation of dendritic spine morphogenesis	Any process that decreases the rate, frequency, or extent of dendritic spine morphogenesis, the process in which the anatomical structures of a dendritic spine are generated and organized. A dendritic spine is a protrusion from a dendrite and a specialized subcellular compartment involved in synaptic transmission.
negative regulation of epithelial to mesenchymal transition	Any process that decreases the rate, frequency, or extent of epithelial to mesenchymal transition. Epithelial to mesenchymal transition where an epithelial cell loses apical/basolateral polarity, severs intercellular adhesive junctions, degrades basement membrane components and becomes a migratory mesenchymal cell.
negative regulation of MAPK cascade	Any process that stops, prevents, or reduces the frequency, rate or extent of signal transduction mediated by the MAPKKK cascade.
negative regulation of proteolysis involved in protein catabolic process	Any process that stops, prevents or reduces the frequency, rate or extent of proteolysis involved in protein catabolic process.
negative regulation of thymocyte apoptotic process	Any process that stops, prevents, or reduces the frequency, rate or extent of thymocyte death by apoptotic process.
negative regulation of transcription by RNA polymerase II	Any process that stops, prevents, or reduces the frequency, rate or extent of transcription mediated by RNA polymerase II.
notochord formation	The formation of the notochord from the chordamesoderm. The notochord is composed of large cells packed within a firm connective tissue sheath and is found in all chordates at the ventral surface of the neural tube. In vertebrates, the notochord contributes to the vertebral column.
positive regulation of amyloid-beta formation	Any process that activates or increases the frequency, rate or extent of amyloid-beta formation.
positive regulation of aspartic-type endopeptidase activity involved in amyloid precursor protein catabolic process	Any process that activates or increases the frequency, rate or extent of aspartic-type endopeptidase activity involved in amyloid precursor protein catabolic process.
positive regulation of MAPK cascade	Any process that activates or increases the frequency, rate or extent of signal transduction mediated by the MAPK cascade.
positive regulation of peptidyl-tyrosine phosphorylation	Any process that activates or increases the frequency, rate or extent of the phosphorylation of peptidyl-tyrosine.
positive regulation of protein phosphorylation	Any process that activates or increases the frequency, rate or extent of addition of phosphate groups to amino acids within a protein.
positive regulation of protein tyrosine kinase activity	Any process that increases the rate, frequency, or extent of protein tyrosine kinase activity.
protein stabilization	Any process involved in maintaining the structure and integrity of a protein and preventing it from degradation or aggregation.
regulation of angiogenesis	Any process that modulates the frequency, rate or extent of angiogenesis.
regulation of axonogenesis	Any process that modulates the frequency, rate or extent of axonogenesis, the generation of an axon, the long process of a neuron.
regulation of blood vessel endothelial cell migration	Any process that modulates the frequency, rate or extent of the migration of the endothelial cells of blood vessels.
regulation of cell adhesion mediated by integrin	Any process that modulates the frequency, rate, or extent of cell adhesion mediated by integrin.
regulation of peptidyl-tyrosine phosphorylation	Any process that modulates the frequency, rate or extent of the phosphorylation of peptidyl-tyrosine.
substrate adhesion-dependent cell spreading	The morphogenetic process that results in flattening of a cell as a consequence of its adhesion to a substrate.

2 homologous proteins in AiPD

UniProt AC	Gene Name	Protein Name	Species	Evidence Code
Q3ZC64	EFNA1	Ephrin-A1	Bos taurus (Bovine)	PR
Q06AS9	EFNA1	Ephrin-A1	Sus scrofa (Pig)	PR

10	20	30	40	50	60
MEFLWAPLLG	LCCSLAAADR	HTVFWNSSNP	KFRNEDYTIH	VQLNDYVDII	CPHYEDHSVA
70	80	90	100	110	120
DAAMEQYILY	LVEHEEYQLC	QPQSKDQVRW	QCNRPSAKHG	PEKLSEKFQR	FTPFTLGKEF
130	140	150	160	170	180
KEGHSYYYIS	KPIHQHEDRC	LRLKVTVSGK	ITHSPQAHDN	PQEKRLAADD	PEVRVLHSIG
190	200
HSAAPRLFPL	AWTVLLLPLL	LLQTP