P0AG24

PR
Gene name
spoT (b3650, JW3625)
Protein name
Bifunctional (p)ppGpp synthase/hydrolase SpoT
Names
Species
Escherichia coli (strain K12)
KEGG Pathway
eco:b3650
EC number
2.7.6.5: Diphosphotransferases
Protein Class

Descriptions

The autoinhibited protein was predicted that may have potential autoinhibitory elements via cis-regPred.

Autoinhibitory domains (AIDs)

515-584 (Predicted disordered autoinhibitory region) PR

Target domain

Relief mechanism

Assay

cis-regPred

Accessory elements

No accessory elements

References

Autoinhibited structure

Activated structure

1 structures for P0AG24

Entry ID Method Resolution Chain Position Source
AF-P0AG24-F1 Predicted AlphaFoldDB

No variants for P0AG24

Variant ID(s) Position Change Description Diseaes Association Provenance
No variants for P0AG24

No associated diseases with P0AG24

7 regional properties for P0AG24

Type Name Position InterPro Accession
domain ACT domain 622 - 702 IPR002912
domain HD/PDEase domain 41 - 166 IPR003607
domain TGS 386 - 447 IPR004095
domain HD domain 45 - 144 IPR006674
domain RelA/SpoT 214 - 345 IPR007685
domain RelA/SpoT, TGS domain 389 - 447 IPR033655
domain RelA/SpoT, AH and RIS domains 459 - 541 IPR045600

Functions

Description
EC Number 2.7.6.5 Diphosphotransferases
Subcellular Localization
  • Cytoplasm
  • Is associated with pre-50S ribosomal subunits in a salt-dependent manner
PANTHER Family
PANTHER Subfamily
PANTHER Protein Class
PANTHER Pathway Category No pathway information available

1 GO annotations of cellular component

Name Definition
cytosol The part of the cytoplasm that does not contain organelles but which does contain other particulate matter, such as protein complexes.

3 GO annotations of molecular function

Name Definition
GTP diphosphokinase activity Catalysis of the reaction: ATP + GTP = AMP + guanosine 3'-diphosphate 5'-triphosphate.
guanosine-3',5'-bis(diphosphate) 3'-diphosphatase activity Catalysis of the reaction: guanosine 3',5'-bis(diphosphate) + H2O = guanosine 5'-diphosphate + diphosphate.
kinase activity Catalysis of the transfer of a phosphate group, usually from ATP, to a substrate molecule.

5 GO annotations of biological process

Name Definition
guanosine tetraphosphate biosynthetic process The chemical reactions and pathways resulting in the formation of guanine tetraphosphate (5'-ppGpp-3'), a derivative of guanine riboside with four phosphates.
guanosine tetraphosphate metabolic process The chemical reactions and pathways involving guanine tetraphosphate (5'-ppGpp-3'), a derivative of guanine riboside with four phosphates.
nucleobase-containing small molecule interconversion The chemical reactions and pathways by which a nucleobase, nucleoside or nucleotide small molecule is synthesized from another nucleobase, nucleoside or nucleotide small molecule.
phosphorylation The process of introducing a phosphate group into a molecule, usually with the formation of a phosphoric ester, a phosphoric anhydride or a phosphoric amide.
response to starvation Any process that results in a change in state or activity of a cell or an organism (in terms of movement, secretion, enzyme production, gene expression, etc.) as a result of a starvation stimulus, deprivation of nourishment.

2 homologous proteins in AiPD

UniProt AC Gene Name Protein Name Species Evidence Code
Q67UU0 RSH3 Probable GTP diphosphokinase RSH3, chloroplastic Oryza sativa subsp japonica (Rice) PR
Q7XAP4 RSH2 Probable GTP diphosphokinase RSH2, chloroplastic Oryza sativa subsp japonica (Rice) PR
10 20 30 40 50 60
MYLFESLNQL IQTYLPEDQI KRLRQAYLVA RDAHEGQTRS SGEPYITHPV AVACILAEMK
70 80 90 100 110 120
LDYETLMAAL LHDVIEDTPA TYQDMEQLFG KSVAELVEGV SKLDKLKFRD KKEAQAENFR
130 140 150 160 170 180
KMIMAMVQDI RVILIKLADR THNMRTLGSL RPDKRRRIAR ETLEIYSPLA HRLGIHHIKT
190 200 210 220 230 240
ELEELGFEAL YPNRYRVIKE VVKAARGNRK EMIQKILSEI EGRLQEAGIP CRVSGREKHL
250 260 270 280 290 300
YSIYCKMVLK EQRFHSIMDI YAFRVIVNDS DTCYRVLGQM HSLYKPRPGR VKDYIAIPKA
310 320 330 340 350 360
NGYQSLHTSM IGPHGVPVEV QIRTEDMDQM AEMGVAAHWA YKEHGETSTT AQIRAQRWMQ
370 380 390 400 410 420
SLLELQQSAG SSFEFIESVK SDLFPDEIYV FTPEGRIVEL PAGATPVDFA YAVHTDIGHA
430 440 450 460 470 480
CVGARVDRQP YPLSQPLTSG QTVEIITAPG ARPNAAWLNF VVSSKARAKI RQLLKNLKRD
490 500 510 520 530 540
DSVSLGRRLL NHALGGSRKL NEIPQENIQR ELDRMKLATL DDLLAEIGLG NAMSVVVAKN
550 560 570 580 590 600
LQHGDASIPP ATQSHGHLPI KGADGVLITF AKCCRPIPGD PIIAHVSPGK GLVIHHESCR
610 620 630 640 650 660
NIRGYQKEPE KFMAVEWDKE TAQEFITEIK VEMFNHQGAL ANLTAAINTT TSNIQSLNTE
670 680 690 700
EKDGRVYSAF IRLTARDRVH LANIMRKIRV MPDVIKVTRN RN