P05055

PR
Gene name
pnp
Protein name
Polyribonucleotide nucleotidyltransferase
Names
Polynucleotide phosphorylase, PNPase
Species
Escherichia coli (strain K12)
KEGG Pathway
eco:b3164
EC number
2.7.7.8: Nucleotidyltransferases
Protein Class

Descriptions

The autoinhibited protein was predicted that may have potential autoinhibitory elements via cis-regPred.

Autoinhibitory domains (AIDs)

659-711 (Predicted disordered autoinhibitory region) PR

Target domain

Relief mechanism

Assay

cis-regPred

Accessory elements

No accessory elements

References

Autoinhibited structure

Activated structure

12 structures for P05055

Entry ID Method Resolution Chain Position Source
1SRO NMR - A 617-692 PDB
3CDI X-ray 260 A A 2-711 PDB
3CDJ X-ray 280 A A 2-547 PDB
3GCM X-ray 250 A A/B/C 1-549 PDB
3GLL X-ray 270 A A 1-549 PDB
3GME X-ray 240 A A 1-549 PDB
3H1C X-ray 357 A A/B/C/G/I/K/M/O/R/T/V/X 1-549 PDB
6QH2 NMR - A 545-711 PDB
7OGK EM 340 A A/B/C 1-711 PDB
7OGL EM 340 A A/B/C 1-711 PDB
7OGM EM 370 A L/N/O 1-711 PDB
AF-P05055-F1 Predicted AlphaFoldDB

No variants for P05055

Variant ID(s) Position Change Description Diseaes Association Provenance
No variants for P05055

No associated diseases with P05055

9 regional properties for P05055

Type Name Position InterPro Accession
domain Exoribonuclease, phosphorolytic domain 1 16 - 144 IPR001247-1
domain Exoribonuclease, phosphorolytic domain 1 324 - 456 IPR001247-2
domain S1 domain 619 - 690 IPR003029
domain K Homology domain 552 - 617 IPR004087
domain K Homology domain, type 1 556 - 614 IPR004088
domain Exoribonuclease, phosphorolytic domain 2 147 - 210 IPR015847-1
domain Exoribonuclease, phosphorolytic domain 2 460 - 527 IPR015847-2
domain Polyribonucleotide nucleotidyltransferase, RNA-binding domain 242 - 320 IPR015848
domain RNA-binding domain, S1 620 - 690 IPR022967

Functions

Description
EC Number 2.7.7.8 Nucleotidyltransferases
Subcellular Localization
  • Cytoplasm
  • Has also been isolated in association with the inner membrane
PANTHER Family
PANTHER Subfamily
PANTHER Protein Class
PANTHER Pathway Category No pathway information available

3 GO annotations of cellular component

Name Definition
bacterial degradosome The degradosome is a protein complex playing a key role in mRNA degradation and RNA processing. It includes a RNA helicase, a 3'-5' phosphate-dependent PNPase and a RNase E bound-enolase.
cytosol The part of the cytoplasm that does not contain organelles but which does contain other particulate matter, such as protein complexes.
membrane A lipid bilayer along with all the proteins and protein complexes embedded in it an attached to it.

6 GO annotations of molecular function

Name Definition
3'-5'-exoribonuclease activity Catalysis of the sequential cleavage of mononucleotides from a free 3' terminus of an RNA molecule.
cyclic-di-GMP binding Binding to cyclic-di-GMP, cyclic dimeric guanosine monophosphate.
identical protein binding Binding to an identical protein or proteins.
magnesium ion binding Binding to a magnesium (Mg) ion.
polyribonucleotide nucleotidyltransferase activity Catalysis of the reaction: RNA(n+1) + phosphate <=> RNA(n) + a nucleoside diphosphate.
RNA binding Binding to an RNA molecule or a portion thereof.

4 GO annotations of biological process

Name Definition
mRNA catabolic process The chemical reactions and pathways resulting in the breakdown of mRNA, messenger RNA, which is responsible for carrying the coded genetic 'message', transcribed from DNA, to sites of protein assembly at the ribosomes.
response to heat Any process that results in a change in state or activity of a cell or an organism (in terms of movement, secretion, enzyme production, gene expression, etc.) as a result of a heat stimulus, a temperature stimulus above the optimal temperature for that organism.
RNA catabolic process The chemical reactions and pathways resulting in the breakdown of RNA, ribonucleic acid, one of the two main type of nucleic acid, consisting of a long, unbranched macromolecule formed from ribonucleotides joined in 3',5'-phosphodiester linkage.
RNA processing Any process involved in the conversion of one or more primary RNA transcripts into one or more mature RNA molecules.

3 homologous proteins in AiPD

UniProt AC Gene Name Protein Name Species Evidence Code
Q8TCS8 PNPT1 Polyribonucleotide nucleotidyltransferase 1, mitochondrial Homo sapiens (Human) PR
Q8K1R3 Pnpt1 Polyribonucleotide nucleotidyltransferase 1, mitochondrial Mus musculus (Mouse) PR
Q6KAI0 PNP2 Polyribonucleotide nucleotidyltransferase 2, mitochondrial Oryza sativa subsp japonica (Rice) PR
10 20 30 40 50 60
MLNPIVRKFQ YGQHTVTLET GMMARQATAA VMVSMDDTAV FVTVVGQKKA KPGQDFFPLT
70 80 90 100 110 120
VNYQERTYAA GRIPGSFFRR EGRPSEGETL IARLIDRPIR PLFPEGFVNE VQVIATVVSV
130 140 150 160 170 180
NPQVNPDIVA MIGASAALSL SGIPFNGPIG AARVGYINDQ YVLNPTQDEL KESKLDLVVA
190 200 210 220 230 240
GTEAAVLMVE SEAQLLSEDQ MLGAVVFGHE QQQVVIQNIN ELVKEAGKPR WDWQPEPVNE
250 260 270 280 290 300
ALNARVAALA EARLSDAYRI TDKQERYAQV DVIKSETIAT LLAEDETLDE NELGEILHAI
310 320 330 340 350 360
EKNVVRSRVL AGEPRIDGRE KDMIRGLDVR TGVLPRTHGS ALFTRGETQA LVTATLGTAR
370 380 390 400 410 420
DAQVLDELMG ERTDTFLFHY NFPPYSVGET GMVGSPKRRE IGHGRLAKRG VLAVMPDMDK
430 440 450 460 470 480
FPYTVRVVSE ITESNGSSSM ASVCGASLAL MDAGVPIKAA VAGIAMGLVK EGDNYVVLSD
490 500 510 520 530 540
ILGDEDHLGD MDFKVAGSRD GISALQMDIK IEGITKEIMQ VALNQAKGAR LHILGVMEQA
550 560 570 580 590 600
INAPRGDISE FAPRIHTIKI NPDKIKDVIG KGGSVIRALT EETGTTIEIE DDGTVKIAAT
610 620 630 640 650 660
DGEKAKHAIR RIEEITAEIE VGRVYTGKVT RIVDFGAFVA IGGGKEGLVH ISQIADKRVE
670 680 690 700 710
KVTDYLQMGQ EVPVKVLEVD RQGRIRLSIK EATEQSQPAA APEAPAAEQG E