O89043

PR
Gene name
Pola2
Protein name
DNA polymerase alpha subunit B
Names
DNA polymerase alpha 70 kDa subunit, DNA polymerase subunit II
Species
Rattus norvegicus (Rat)
KEGG Pathway
EC number
Protein Class

Descriptions

The autoinhibited protein was predicted that may have potential autoinhibitory elements via cis-regPred.

Autoinhibitory domains (AIDs)

40-225 (Predicted disordered autoinhibitory region) PR

Target domain

Relief mechanism

Assay

cis-regPred

Accessory elements

No accessory elements

References

Autoinhibited structure

Activated structure

1 structures for O89043

Entry ID Method Resolution Chain Position Source
AF-O89043-F1 Predicted AlphaFoldDB

No variants for O89043

Variant ID(s) Position Change Description Diseaes Association Provenance
No variants for O89043

No associated diseases with O89043

2 regional properties for O89043

Type Name Position InterPro Accession
domain DNA polymerase alpha/delta/epsilon, subunit B 342 - 549 IPR007185
domain DNA polymerase alpha, subunit B, N-terminal 22 - 239 IPR013627

Functions

Description
EC Number
Subcellular Localization
  • Nucleus
PANTHER Family
PANTHER Subfamily
PANTHER Protein Class
PANTHER Pathway Category No pathway information available

2 GO annotations of cellular component

Name Definition
alpha DNA polymerase:primase complex A complex of four polypeptides, comprising large and small DNA polymerase alpha subunits and two primase subunits, which are capable of catalyzing the synthesis of an RNA primer on the lagging strand of replicating DNA and the subsequent synthesis of a smal stretch of DNA. The smaller of the two primase subunits alone can catalyze oligoribonucleotide synthesis.
nucleus A membrane-bounded organelle of eukaryotic cells in which chromosomes are housed and replicated. In most cells, the nucleus contains all of the cell's chromosomes except the organellar chromosomes, and is the site of RNA synthesis and processing. In some species, or in specialized cell types, RNA metabolism or DNA replication may be absent.

2 GO annotations of molecular function

Name Definition
DNA binding Any molecular function by which a gene product interacts selectively and non-covalently with DNA (deoxyribonucleic acid).
DNA-directed DNA polymerase activity Catalysis of the reaction: deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1); the synthesis of DNA from deoxyribonucleotide triphosphates in the presence of a DNA template and a 3'hydroxyl group.

4 GO annotations of biological process

Name Definition
DNA replication The cellular metabolic process in which a cell duplicates one or more molecules of DNA. DNA replication begins when specific sequences, known as origins of replication, are recognized and bound by initiation proteins, and ends when the original DNA molecule has been completely duplicated and the copies topologically separated. The unit of replication usually corresponds to the genome of the cell, an organelle, or a virus. The template for replication can either be an existing DNA molecule or RNA.
DNA replication initiation The process in which DNA-dependent DNA replication is started; this begins with the ATP dependent loading of an initiator complex onto the DNA, this is followed by DNA melting and helicase activity. In bacteria, the gene products that enable the helicase activity are loaded after the initial melting and in archaea and eukaryotes, the gene products that enable the helicase activity are inactive when they are loaded and subsequently activate.
DNA replication, synthesis of RNA primer The synthesis of a short RNA polymer, usually 4-15 nucleotides long, using one strand of unwound DNA as a template; the RNA then serves as a primer from which DNA polymerases extend synthesis.
protein import into nucleus The directed movement of a protein from the cytoplasm to the nucleus.

3 homologous proteins in AiPD

UniProt AC Gene Name Protein Name Species Evidence Code
P38121 POL12 DNA polymerase alpha subunit B Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) PR
Q14181 POLA2 DNA polymerase alpha subunit B Homo sapiens (Human) PR
P33611 Pola2 DNA polymerase alpha subunit B Mus musculus (Mouse) PR
10 20 30 40 50 60
MAVSTQQLAE ELQIFGLDCE DSLLEKLAEL CTLYRQTEER MVSELIAFCT SAGKSCLTGE
70 80 90 100 110 120
ILSSFEHEVL NKKLSKACHS ASKDNRHAGA RDIVSIQELI EAEEEEETLL SAYTTPSKGP
130 140 150 160 170 180
HKRVSSTPET PLTKRSISTR SPHQLLSPSS FSPSATPSQK YSSRTNRGEV VTTFGSAQGV
190 200 210 220 230 240
SWSGRGGSGS ISLKVMGYPE PLTSSYKTMF QQLPDIREVL TCKIEELGSE LKEHYKIEAF
250 260 270 280 290 300
TPLLVPAQEP VILLGQIGCD SNGRLNSKSV ILEGDREHSS GAQIPVDVSE LKDYSLFPGQ
310 320 330 340 350 360
VVIMEGFNTT GRRLTATKLY EGVPLPFYQP TEEEGDFEQT MVLVACGPYT TSDSITYDPL
370 380 390 400 410 420
LDLISTINHD RPDVCILFGP FLDAKHEQVE NCKLTSPFED IFKQCLRTVI EGTRSSGSHL
430 440 450 460 470 480
VFVPSLRDVH HEPVYPQPPF TFSELPREDK KRVQFVSEPC NLSINGVMFG LTSTDLLFHI
490 500 510 520 530 540
GAEEICSSSG TSDRFSRILK HILTQRSYYP LYPPHEDMAI DYENFYTYAQ LPVTPDVFIV
550 560 570 580 590
PSELRYFVKD IFGCVCMNPG RLTKGQVGGT FGRLYLRRQP KGTDSEGRQG LSVAAQVVRI