O23247

PR
Gene name
EMB1027
Protein name
Arginine--tRNA ligase, chloroplastic/mitochondrial
Names
Arginyl-tRNA synthetase, ArgRS, Protein EMBRYO DEFECTIVE 1027
Species
Arabidopsis thaliana (Mouse-ear cress)
KEGG Pathway
ath:AT4G26300
EC number
6.1.1.19: Ligases forming aminoacyl-tRNA and related compounds
Protein Class

Descriptions

The autoinhibited protein was predicted that may have potential autoinhibitory elements via cis-regPred.

Autoinhibitory domains (AIDs)

441-516 (Predicted disordered autoinhibitory region) PR

Target domain

Relief mechanism

Assay

cis-regPred

Accessory elements

No accessory elements

References

Autoinhibited structure

Activated structure

1 structures for O23247

Entry ID Method Resolution Chain Position Source
AF-O23247-F1 Predicted AlphaFoldDB

27 variants for O23247

Variant ID(s) Position Change Description Diseaes Association Provenance
ENSVATH02926198 2 F>S No 1000Genomes
ENSVATH14298144 4 F>L No 1000Genomes
ENSVATH02926197 7 D>Y No 1000Genomes
ENSVATH06778803 10 R>S No 1000Genomes
ENSVATH06778802 11 R>* No 1000Genomes
tmp_4_13313063_G_A 16 T>I No 1000Genomes
ENSVATH09498815 23 D>N No 1000Genomes
ENSVATH06778801 27 F>L No 1000Genomes
ENSVATH12239123 29 T>A No 1000Genomes
ENSVATH12239123 29 T>S No 1000Genomes
tmp_4_13312082_G_C 65 R>G No 1000Genomes
ENSVATH02926165 83 P>T No 1000Genomes
ENSVATH06778790 97 F>Y No 1000Genomes
tmp_4_13311380_C_A 211 A>S No 1000Genomes
ENSVATH02926154 220 E>D No 1000Genomes
ENSVATH02926150 249 D>E No 1000Genomes
ENSVATH02926149 250 S>T No 1000Genomes
ENSVATH12238969 268 H>Q No 1000Genomes
ENSVATH06778766 314 Q>R No 1000Genomes
ENSVATH02926140 403 Q>R No 1000Genomes
ENSVATH06778756 423 D>E No 1000Genomes
ENSVATH12238929 428 R>K No 1000Genomes
tmp_4_13309742_G_A 481 P>L No 1000Genomes
tmp_4_13308884_G_A 551 T>I No 1000Genomes
ENSVATH02926126 574 T>S No 1000Genomes
tmp_4_13308664_T_A 593 N>I No 1000Genomes
tmp_4_13308435_G_A 632 H>Y No 1000Genomes

No associated diseases with O23247

4 regional properties for O23247

Type Name Position InterPro Accession
conserved_site Aminoacyl-tRNA synthetase, class I, conserved site 190 - 201 IPR001412
domain Arginyl tRNA synthetase N-terminal domain 65 - 154 IPR005148
domain DALR anticodon binding 527 - 642 IPR008909
domain Arginyl-tRNA synthetase, catalytic core domain 163 - 513 IPR035684

Functions

Description
EC Number 6.1.1.19 Ligases forming aminoacyl-tRNA and related compounds
Subcellular Localization
  • Plastid, chloroplast
  • Mitochondrion
PANTHER Family
PANTHER Subfamily
PANTHER Protein Class
PANTHER Pathway Category No pathway information available

4 GO annotations of cellular component

Name Definition
chloroplast A chlorophyll-containing plastid with thylakoids organized into grana and frets, or stroma thylakoids, and embedded in a stroma.
chloroplast stroma The space enclosed by the double membrane of a chloroplast but excluding the thylakoid space. It contains DNA, ribosomes and some temporary products of photosynthesis.
cytosol The part of the cytoplasm that does not contain organelles but which does contain other particulate matter, such as protein complexes.
mitochondrion A semiautonomous, self replicating organelle that occurs in varying numbers, shapes, and sizes in the cytoplasm of virtually all eukaryotic cells. It is notably the site of tissue respiration.

2 GO annotations of molecular function

Name Definition
arginine-tRNA ligase activity Catalysis of the reaction: ATP + L-arginine + tRNA(Arg) = AMP + diphosphate + L-arginyl-tRNA(Arg).
ATP binding Binding to ATP, adenosine 5'-triphosphate, a universally important coenzyme and enzyme regulator.

2 GO annotations of biological process

Name Definition
arginyl-tRNA aminoacylation The process of coupling arginine to arginyl-tRNA, catalyzed by arginyl-tRNA synthetase. The arginyl-tRNA synthetase is a class-I synthetase. The activated amino acid is transferred to the 2'-OH group of an alanine accetping tRNA. The 2'-O-aminoacyl-tRNA will ultimately migrate to the 3' position via transesterification.
embryo development ending in seed dormancy The process whose specific outcome is the progression of the embryo over time, from zygote formation to the end of seed dormancy. An example of this process is found in Arabidopsis thaliana.

6 homologous proteins in AiPD

UniProt AC Gene Name Protein Name Species Evidence Code
Q05506 YDR341C Arginine--tRNA ligase, cytoplasmic Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) PR
Q0P5H7 RARS2 Probable arginine--tRNA ligase, mitochondrial Bos taurus (Bovine) PR
Q9VXN4 ArgRS Probable arginine--tRNA ligase, cytoplasmic Drosophila melanogaster (Fruit fly) PR
Q5T160 RARS2 Probable arginine--tRNA ligase, mitochondrial Homo sapiens (Human) PR
Q3U186 Rars2 Probable arginine--tRNA ligase, mitochondrial Mus musculus (Mouse) PR
Q9C713 At1g66530 Arginine--tRNA ligase, cytoplasmic Arabidopsis thaliana (Mouse-ear cress) PR
10 20 30 40 50 60
MFIFPKDENR RETLTTKLRF SADHLTFTTV TEKLRATAWR FAFSSRAKSV VAMAANEEFT
70 80 90 100 110 120
GNLKRQLAKL FDVSLKLTVP DEPSVEPLVA ASALGKFGDY QCNNAMGLWS IIKGKGTQFK
130 140 150 160 170 180
GPPAVGQALV KSLPTSEMVE SCSVAGPGFI NVVLSAKWMA KSIENMLIDG VDTWAPTLSV
190 200 210 220 230 240
KRAVVDFSSP NIAKEMHVGH LRSTIIGDTL ARMLEYSHVE VLRRNHVGDW GTQFGMLIEY
250 260 270 280 290 300
LFEKFPDTDS VTETAIGDLQ VFYKASKHKF DLDEAFKEKA QQAVVRLQGG DPVYRKAWAK
310 320 330 340 350 360
ICDISRTEFA KVYQRLRVEL EEKGESFYNP HIAKVIEELN SKGLVEESEG ARVIFLEGFD
370 380 390 400 410 420
IPLMVVKSDG GFNYASTDLT ALWYRLNEEK AEWIIYVTDV GQQQHFNMFF KAARKAGWLP
430 440 450 460 470 480
DNDKTYPRVN HVGFGLVLGE DGKRFRTRAT DVVRLVDLLD EAKTRSKLAL IERGKDKEWT
490 500 510 520 530 540
PEELDQTAEA VGYGAVKYAD LKNNRLTNYT FSFDQMLNDK GNTAVYLLYA HARICSIIRK
550 560 570 580 590 600
SGKDIDELKK TGKLALDHAD ERALGLHLLR FAETVEEACT NLLPSVLCEY LYNLSEHFTR
610 620 630 640
FYSNCQVNGS PEETSRLLLC EATAIVMRKC FHLLGITPVY KI