D3ZBE5
Gene name |
Nek7 |
Protein name |
Serine/threonine-protein kinase Nek7 |
Names |
Never in mitosis A-related kinase 7, NimA-related protein kinase 7 |
Species |
Mus musculus (Mouse) |
KEGG Pathway |
rno:360850 |
EC number |
2.3.2.26: Aminoacyltransferases |
Protein Class |
|
Descriptions
(Annotation based on sequence homology with Q9HAU4)
Ubiquitination of proteins is an abundant modification that controls numerous cellular processes. The C2-WW-HECT-domain E3 Smurf2 downregulates transforming growth factor-β (TGF-β) signaling by targeting itself, the adaptor protein Smad7, and TGF-β receptor kinases for degradation. The intramolecular interaction between C2 phospholipid binding domain and HECT domain inhibits the catalytic activity of the HECT domain by obstructing accessibility of the catalytic cysteine of the HECT domain and thus blocking Smurf2-Ub thioester formation. The autoinhibition is relieved by the binding of HECT-binding domain of Smad7.
Autoinhibitory domains (AIDs)
Target domain |
20-30 (N-terminal extension of kinase domain) |
Relief mechanism |
Partner binding |
Assay |
|
Accessory elements
178-201 (Activation loop from InterPro)
Target domain |
34-299 (Protein kinase domain) |
Relief mechanism |
|
Assay |
|
Autoinhibited structure
Activated structure
1 structures for D3ZBE5
| Entry ID | Method | Resolution | Chain | Position | Source |
|---|---|---|---|---|---|
| AF-D3ZBE5-F1 | Predicted | AlphaFoldDB |
No variants for D3ZBE5
| Variant ID(s) | Position | Change | Description | Diseaes Association | Provenance |
|---|---|---|---|---|---|
| No variants for D3ZBE5 | |||||
No associated diseases with D3ZBE5
5 regional properties for D3ZBE5
| Type | Name | Position | InterPro Accession |
|---|---|---|---|
| domain | Protein kinase domain | 12 - 264 | IPR000719 |
| domain | NAF domain | 294 - 351 | IPR004041 |
| active_site | Serine/threonine-protein kinase, active site | 130 - 142 | IPR008271 |
| binding_site | Protein kinase, ATP binding site | 18 - 41 | IPR017441 |
| domain | NAF/FISL domain | 291 - 315 | IPR018451 |
Functions
| Description | ||
|---|---|---|
| EC Number | 2.3.2.26 | Aminoacyltransferases |
| Subcellular Localization |
|
|
| PANTHER Family | ||
| PANTHER Subfamily | ||
| PANTHER Protein Class | ||
| PANTHER Pathway Category | No pathway information available | |
4 GO annotations of cellular component
| Name | Definition |
|---|---|
| cytoplasm | The contents of a cell excluding the plasma membrane and nucleus, but including other subcellular structures. |
| microtubule organizing center | An intracellular structure that can catalyze gamma-tubulin-dependent microtubule nucleation and that can anchor microtubules by interacting with their minus ends, plus ends or sides. |
| nucleus | A membrane-bounded organelle of eukaryotic cells in which chromosomes are housed and replicated. In most cells, the nucleus contains all of the cell's chromosomes except the organellar chromosomes, and is the site of RNA synthesis and processing. In some species, or in specialized cell types, RNA metabolism or DNA replication may be absent. |
| spindle pole | Either of the ends of a spindle, where spindle microtubules are organized; usually contains a microtubule organizing center and accessory molecules, spindle microtubules and astral microtubules. |
5 GO annotations of molecular function
| Name | Definition |
|---|---|
| ATP binding | Binding to ATP, adenosine 5'-triphosphate, a universally important coenzyme and enzyme regulator. |
| metal ion binding | Binding to a metal ion. |
| protein serine kinase activity | Catalysis of the reactions: ATP + protein serine = ADP + protein serine phosphate. |
| protein serine/threonine kinase activity | Catalysis of the reactions: ATP + protein serine = ADP + protein serine phosphate, and ATP + protein threonine = ADP + protein threonine phosphate. |
| protein serine/threonine/tyrosine kinase activity | Catalysis of the reactions: ATP + a protein serine = ADP + protein serine phosphate; ATP + a protein threonine = ADP + protein threonine phosphate; and ATP + a protein tyrosine = ADP + protein tyrosine phosphate. |
6 GO annotations of biological process
| Name | Definition |
|---|---|
| cellular response to potassium ion | Any process that results in a change in state or activity of a cell (in terms of movement, secretion, enzyme production, gene expression, etc.) as a result of a potassium ion stimulus. |
| positive regulation of NLRP3 inflammasome complex assembly | Any process that activates or increases the frequency, rate or extent of NLRP3 inflammasome complex assembly. |
| positive regulation of telomerase activity | Any process that activates or increases the frequency, rate or extent of telomerase activity, the catalysis of the reaction: deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1). |
| positive regulation of telomere capping | Any process that activates or increases the frequency, rate or extent of telomere capping. |
| positive regulation of telomere maintenance via telomerase | Any process that activates or increases the frequency, rate or extent of the addition of telomeric repeats by telomerase. |
| protein phosphorylation | The process of introducing a phosphate group on to a protein. |
9 homologous proteins in AiPD
| UniProt AC | Gene Name | Protein Name | Species | Evidence Code |
|---|---|---|---|---|
| Q9HC98 | NEK6 | Serine/threonine-protein kinase Nek6 | Homo sapiens (Human) | EV |
| Q8TDX7 | NEK7 | Serine/threonine-protein kinase Nek7 | Homo sapiens (Human) | EV |
| Q9ES70 | Nek6 | Serine/threonine-protein kinase Nek6 | Mus musculus (Mouse) | SS |
| Q9ES74 | Nek7 | Serine/threonine-protein kinase Nek7 | Mus musculus (Mouse) | SS |
| A2BD05 | NEK6 | Serine/threonine-protein kinase Nek6 | Sus scrofa (Pig) | SS |
| P59895 | Nek6 | Serine/threonine-protein kinase Nek6 | Rattus norvegicus (Rat) | SS |
| G5EFM9 | nekl-3 | Serine/threonine-protein kinase nekl-3 | Caenorhabditis elegans | PR |
| Q9LVL5 | WNK4 | Probable serine/threonine-protein kinase WNK4 | Arabidopsis thaliana (Mouse-ear cress) | PR |
| Q9STK6 | WNK3 | Probable serine/threonine-protein kinase WNK3 | Arabidopsis thaliana (Mouse-ear cress) | PR |
| 10 | 20 | 30 | 40 | 50 | 60 |
| MSNPGTRRNG | SSIKIRLTVL | CAKNLAKKDF | FRLPDPFAKI | VVDGSGQCHS | TDTVKNTLDP |
| 70 | 80 | 90 | 100 | 110 | 120 |
| KWNQHYDLYV | GKTDSITISV | WNHKKIHKKQ | GAGFLGCVRL | LSNAISRLKD | TGYQRLDLCK |
| 130 | 140 | 150 | 160 | 170 | 180 |
| LNPSDTDAVR | GQIVVSLQTR | DRIGGGGSVV | DCRGLLENEG | TVYEDSGPGR | PLSCLMEEPA |
| 190 | 200 | 210 | 220 | 230 | 240 |
| PYTDGTGAAA | GGGNCRFVES | PSQDQRLLVQ | RLRNPEVRGP | LQTPQNRPHG | HQSPELPEGY |
| 250 | 260 | 270 | 280 | 290 | 300 |
| EQRTTVQGQV | YFLHTQTGVS | TWHDPRIPRD | LNSVNCDELG | PLPPGWEVRS | TVSGRIYFVD |
| 310 | 320 | 330 | 340 | 350 | 360 |
| HNNRTTQFTD | PRLHHIMNHQ | CQLKEPSQPL | QLPSEGSVED | EELPAQRYER | DLVQKLKVLR |
| 370 | 380 | 390 | 400 | 410 | 420 |
| HELSLQQPQA | GHCRIEVSRE | EIFEESYRQI | MKMRPKDLKK | RLMVKFRGEE | GLDYGGVARE |
| 430 | 440 | 450 | 460 | 470 | 480 |
| WLYLLCHEML | NPYYGLFQYS | TDNIYTLQIN | PDSSINPDHL | SYFHFVGRIM | GLAVFHGHYI |
| 490 | 500 | 510 | 520 | 530 | 540 |
| NGGFTVPFYK | QLLGKPIQLS | DLESVDPELH | KSLVWILEND | ITPVLDHTFC | VEHNAFGRIL |
| 550 | 560 | 570 | 580 | 590 | 600 |
| QHELKPNGRN | VPVTEENKKE | YVRLYVNWRF | MRGIEAQFLA | LQKGFNELIP | QHLLKPFDQK |
| 610 | 620 | 630 | 640 | 650 | 660 |
| ELELIIGGLD | KIDLNDWKSN | TRLKHCVADS | NIVRWFWQAV | ETFDEERRAR | LLQFVTGSTR |
| 670 | 680 | 690 | 700 | 710 | 720 |
| VPLQGFKALQ | GSTGAAGPRL | FTIHLIDANT | DNLPKAHTCF | NRIDIPPYES | YEKLYEKLLT |
| 730 | |||||
| AVEETCGFAV | E |